PACE4 (PCSK6) (NM_138323) Human Tagged ORF Clone

CAT#: RG214067

PCSK6 (GFP-tagged) - Human proprotein convertase subtilisin/kexin type 6 (PCSK6), transcript variant 5. Note: ORF is codon optimized

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PCSK6
• Synonyms: PACE4; SPC4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214067 ORF sequence, codon optimized.
  Due to the complexity of NM_138323, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCACCTCGGGCTCCCCCTGCACCAGGCCCCCGACCACCACCCCGAGCTGCTGCTGCAACTGACACCG
  CCGCCGGTGCCGGAGGTGCCGGCGGAGCAGGAGGTGCTGGAGGACCAGGGTTCAGGCCTCTTGCCCCCCG
  CCCTTGGCGATGGTTGCTGTTGCTCGCCCTGCCTGCCGCGTGTTCAGCGCCGCCTCCAAGGCCAGTTTAC
  ACGAACCATTGGGCAGTGCAGGTACTGGGCGGACCTGCAGAGGCAGATCGCGTTGCCGCTGCCCACGGGT
  ACCTCAATCTGGGGCAGATTGGCAACCTCGAGGACTACTACCACTTCTACCACAGCAAAACGTTCAAACG
  GAGCACACTGTCTAGCAGAGGACCCCATACGTTTCTGAGAATGGATCCACAAGTAAAATGGCTGCAGCAG
  CAAGAGGTGAAGCGCAGAGTCAAAAGACAGGTCAGAAGCGACCCTCAGGCTCTGTATTTCAACGACCCTA
  TTTGGAGCAACATGTGGTATCTTCATTGCGGGGATAAGAATTCTCGGTGCAGGTCAGAGATGAATGTGCA
  AGCCGCGTGGAAAAGGGGCTATACTGGAAAAAACGTGGTGGTCACAATCCTTGACGACGGCATTGAGAGA
  AATCACCCGGACCTGGCACCCAATTACGATTCTTATGCAAGCTATGACGTGAACGGCAATGACTATGACC
  CAAGCCCTAGGTACGATGCAAGTAACGAGAACAAGCACGGCACACGGTGCGCTGGCGAGGTCGCTGCTAG
  CGCGAATAATAGCTACTGCATTGTGGGCATCGCCTACAACGCCAAGATCGGTGGAATCCGCATGCTCGAT
  GGCGACGTGACCGACGTGGTGGAAGCCAAGTCCCTCGGCATACGGCCTAACTACATTGACATTTACTCCG
  CATCATGGGGACCGGACGACGATGGTAAGACTGTAGATGGGCCCGGACGATTGGCTAAACAGGCTTTCGA
  GTACGGGATCAAAAAGGGCCGCCAGGGACTGGGCAGCATCTTTGTGTGGGCTAGCGGAAACGGTGGCAGA
  GAGGGAGATTATTGTAGCTGCGACGGGTATACCAACTCTATATATACTATTTCCGTCAGCTCAGCTACTG
  AAAATGGCTACAAGCCTTGGTATCTGGAGGAGTGTGCAAGCACGCTGGCCACCACATACTCATCCGGGGC
  ATTCTACGAACGCAAAATCGTCACAACTGATCTGAGACAACGGTGTACTGACGGCCATACTGGCACCTCT
  GTGAGCGCCCCGATGGTAGCCGGGATCATCGCCCTGGCGCTGGAGGCCAATAGTCAGCTGACCTGGCGCG
  ATGTGCAGCACCTGCTGGTGAAAACTTCTCGCCCAGCCCACTTGAAGGCATCCGACTGGAAAGTGAATGG
  CGCCGGGCACAAAGTGAGCCACTTTTACGGTTTCGGCCTGGTTGATGCTGAGGCATTGGTAGTCGAGGCA
  AAGAAGTGGACCGCCGTGCCCAGTCAGCACATGTGCGTGGCAGCATCCGACAAGAGGCCTCGAAGCATTC
  CCCTGGTGCAGGTGCTGCGCACCACAGCACTGACTTCCGCATGTGCTGAGCATAGCGATCAGAGAGTCGT
  GTATCTTGAACATGTTGTCGTGAGAACGTCAATCTCTCATCCTCGCAGGGGCGACTTGCAGATCTACCTC
  GTGAGTCCGTCTGGGACCAAGTCCCAGCTGCTCGCAAAGCGGCTTCTTGATCTGTCTAACGAGGGCTTCA
  CTAATTGGGAGTTCATGACCGTGCATTGCTGGGGCGAGAAGGCGGAGGGCCAGTGGACGCTCGAGATTCA
  GGACCTGCCCAGCCAGGTCAGAAATCCCGAAAAGCAGGGCAATCTGGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214067 representing NM_138323
  Red=Cloning site Green=Tags(s)
  
  MPPRAPPAPGPRPPPRAAAATDTAAGAGGAGGAGGAGGPGFRPLAPRPWRWLLLLALPAACSAPPPRPVY
  TNHWAVQVLGGPAEADRVAAAHGYLNLGQIGNLEDYYHFYHSKTFKRSTLSSRGPHTFLRMDPQVKWLQQ
  QEVKRRVKRQVRSDPQALYFNDPIWSNMWYLHCGDKNSRCRSEMNVQAAWKRGYTGKNVVVTILDDGIER
  NHPDLAPNYDSYASYDVNGNDYDPSPRYDASNENKHGTRCAGEVAASANNSYCIVGIAYNAKIGGIRMLD
  GDVTDVVEAKSLGIRPNYIDIYSASWGPDDDGKTVDGPGRLAKQAFEYGIKKGRQGLGSIFVWASGNGGR
  EGDYCSCDGYTNSIYTISVSSATENGYKPWYLEECASTLATTYSSGAFYERKIVTTDLRQRCTDGHTGTS
  VSAPMVAGIIALALEANSQLTWRDVQHLLVKTSRPAHLKASDWKVNGAGHKVSHFYGFGLVDAEALVVEA
  KKWTAVPSQHMCVAASDKRPRSIPLVQVLRTTALTSACAEHSDQRVVYLEHVVVRTSISHPRRGDLQIYL
  VSPSGTKSQLLAKRLLDLSNEGFTNWEFMTVHCWGEKAEGQWTLEIQDLPSQVRNPEKQGNLD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138323
• ORF Size: 1869 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_138323.1, NP_612196.1
• RefSeq Size: 3208
• RefSeq ORF: 1872
• Locus ID: 5046
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Gene Summary: This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to the trans-Golgi network where a second autocatalytic event takes place and the catalytic activity is acquired. The encoded protease is constitutively secreted into the extracellular matrix and expressed in many tissues, including neuroendocrine, liver, gut, and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. Some of its substrates include transforming growth factor beta related proteins, proalbumin, and von Willebrand factor. This gene is thought to play a role in tumor progression and left-right patterning. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Feb 2014]