PPP2R2C (NM_181876) Human Tagged ORF Clone

CAT#: RG214086

PPP2R2C (GFP-tagged) - Human protein phosphatase 2, regulatory subunit B, gamma (PPP2R2C), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PPP2R2C
• Synonyms: B55-GAMMA; B55gamma; IMYPNO; IMYPNO1; PR52; PR55G
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214086 representing NM_181876
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCTGTCTTTTTTCTCCAAACACTTGCCCATCCAGGAAGGCCAGCCCTGGGCATTGAAGACTCCAG
  CTGACATCATCTCTACCGTTGAGTTCAACCACACGGGAGAGCTGCTGGCCACAGGTGACAAGGGCGGCCG
  GGTCGTCATCTTCCAGCGGGAACCAGAGAGTAAAAATGCGCCCCACAGCCAGGGCGAATACGACGTGTAC
  AGCACTTTCCAGAGCCACGAGCCGGAGTTTGACTATCTCAAGAGCCTGGAGATAGAGGAGAAGATCAACA
  AGATCAAGTGGCTCCCACAGCAGAACGCCGCCCACTCACTCCTGTCCACCAACGATAAAACTATCAAATT
  ATGGAAGATTACCGAACGAGATAAAAGGCCCGAAGGATACAACCTGAAGGATGAAGAGGGGAAACTTAAG
  GACCTGTCCACGGTGACGTCACTGCAGGTGCCAGTGCTGAAGCCCATGGATCTGATGGTGGAGGTGAGCC
  CTCGGAGGATCTTTGCCAATGGCCACACCTACCACATCAACTCCATCTCCGTCAACAGTGACTGCGAGAC
  CTACATGTCGGCGGATGACCTGCGCATCAACCTCTGGCACCTGGCCATCACCGACAGGAGCTTCAACATC
  GTGGACATCAAGCCGGCCAACATGGAGGACCTTACGGAGGTGATCACAGCATCTGAGTTCCATCCGCACC
  ACTGCAACCTCTTCGTCTACAGCAGCAGCAAGGGCTCCCTGCGGCTCTGCGACATGCGGGCAGCTGCCCT
  GTGTGACAAGCATTCCAAGCTCTTTGAAGAGCCTGAGGACCCCAGTAACCGCTCATTCTTCTCGGAAATC
  ATCTCCTCCGTGTCCGACGTGAAGTTCAGCCACAGCGGCCGCTACATGCTCACCCGGGACTACCTTACAG
  TCAAGGTCTGGGACCTGAACATGGAGGCAAGACCCATAGAGACCTACCAGGTCCATGACTACCTTCGGAG
  CAAGCTCTGTTCCCTGTACGAGAACGACTGCATTTTCGACAAGTTTGAATGTGCCTGGAACGGGAGCGAC
  AGCGTCATCATGACCGGGGCCTACAACAACTTCTTCCGCATGTTCGATCGGAACACCAAGCGGGACGTGA
  CCCTGGAGGCCTCGAGGGAAAGCAGCAAGCCCCGGGCTGTGCTCAAGCCACGGCGCGTGTGCGTGGGGGG
  CAAGCGCCGGCGTGATGACATCAGTGTGGACAGCTTGGACTTCACCAAGAAGATCCTGCACACGGCCTGG
  CACCCGGCTGAGAACATCATTGCCATCGCCGCCACCAACAACCTGTACATCTTCCAGGACAAGGTAAACT
  CTGACATGCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214086 representing NM_181876
  Red=Cloning site Green=Tags(s)
  
  MGLSFFSKHLPIQEGQPWALKTPADIISTVEFNHTGELLATGDKGGRVVIFQREPESKNAPHSQGEYDVY
  STFQSHEPEFDYLKSLEIEEKINKIKWLPQQNAAHSLLSTNDKTIKLWKITERDKRPEGYNLKDEEGKLK
  DLSTVTSLQVPVLKPMDLMVEVSPRRIFANGHTYHINSISVNSDCETYMSADDLRINLWHLAITDRSFNI
  VDIKPANMEDLTEVITASEFHPHHCNLFVYSSSKGSLRLCDMRAAALCDKHSKLFEEPEDPSNRSFFSEI
  ISSVSDVKFSHSGRYMLTRDYLTVKVWDLNMEARPIETYQVHDYLRSKLCSLYENDCIFDKFECAWNGSD
  SVIMTGAYNNFFRMFDRNTKRDVTLEASRESSKPRAVLKPRRVCVGGKRRRDDISVDSLDFTKKILHTAW
  HPAENIIAIAATNNLYIFQDKVNSDMH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181876
• ORF Size: 1341 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181876.1, NP_870991.1
• RefSeq Size: 4087 bp
• RefSeq ORF: 1344 bp
• Locus ID: 5522
• Cytogenetics: 4p16.1
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Tight junction
• Gene Summary: 'The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a gamma isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]'