PPP2R2C (NM_181876) Human Untagged Clone

CAT#: SC127988

PPP2R2C (untagged)-Human protein phosphatase 2, regulatory subunit B, gamma (PPP2R2C), transcript variant 2


  "NM_181876" in other vectors (4)

Reconstitution Protocol

USD 750.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PPP2R2C
Synonyms B55-GAMMA; B55gamma; IMYPNO; IMYPNO1; PR52; PR55G
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_181876, the custom clone sequence may differ by one or more nucleotides


ATGGGGCTGTCTTTTTTCTCCAAACACTTGCCCATCCAGGAAGGCCAGCCCTGGGCATTGAAGACTCCAG
CTGACATCATCTCTACCGTTGAGTTCAACCACACGGGAGAGCTGCTGGCCACAGGTGACAAGGGCGGCCG
GGTCGTCATCTTCCAGCGGGAACCAGAGAGTAAAAATGCGCCCCACAGCCAGGGCGAATACGACGTGTAC
AGCACTTTCCAGAGCCACGAGCCGGAGTTTGACTATCTCAAGAGCCTGGAGATAGAGGAGAAGATCAACA
AGATCAAGTGGCTCCCACAGCAGAACGCCGCCCACTCACTCCTGTCCACCAACGATAAAACTATCAAATT
ATGGAAGATTACCGAACGAGATAAAAGGCCCGAAGGATACAACCTGAAGGATGAAGAGGGGAAACTTAAG
GACCTGTCCACGGTGACGTCACTGCAGGTGCCAGTGCTGAAGCCCATGGATCTGATGGTGGAGGTGAGCC
CTCGGAGGATCTTTGCCAATGGCCACACCTACCACATCAACTCCATCTCCGTCAACAGTGACTGCGAGAC
CTACATGTCGGCGGATGACCTGCGCATCAACCTCTGGCACCTGGCCATCACCGACAGGAGCTTCAACATC
GTGGACATCAAGCCGGCCAACATGGAGGACCTTACGGAGGTGATCACAGCATCTGAGTTCCATCCGCACC
ACTGCAACCTCTTCGTCTACAGCAGCAGCAAGGGCTCCCTGCGGCTCTGCGACATGCGGGCAGCTGCCCT
GTGTGACAAGCATTCCAAGCTCTTTGAAGAGCCTGAGGACCCCAGTAACCGCTCATTCTTCTCGGAAATC
ATCTCCTCCGTGTCCGACGTGAAGTTCAGCCACAGCGGCCGCTACATGCTCACCCGGGACTACCTTACAG
TCAAGGTCTGGGACCTGAACATGGAGGCAAGACCCATAGAGACCTACCAGGTCCATGACTACCTTCGGAG
CAAGCTCTGTTCCCTGTACGAGAACGACTGCATTTTCGACAAGTTTGAATGTGCCTGGAACGGGAGCGAC
AGCGTCATCATGACCGGGGCCTACAACAACTTCTTCCGCATGTTCGATCGGAACACCAAGCGGGACGTGA
CCCTGGAGGCCTCGAGGGAAAGCAGCAAGCCCCGGGCTGTGCTCAAGCCACGGCGCGTGTGCGTGGGGGG
CAAGCGCCGGCGTGATGACATCAGTGTGGACAGCTTGGACTTCACCAAGAAGATCCTGCACACGGCCTGG
CACCCGGCTGAGAACATCATTGCCATCGCCGCCACCAACAACCTGTACATCTTCCAGGACAAGGTAAACT
CTGACATGCACTAG


>OriGene 5' read for NM_181876 unedited
ATTCGGCACGAGGGACGCCCGTCGCGGTCGCAGCCTGCGGAGCCGCCCATGGCCCAGCAG
CCGGCTGACCGCGCCCCCGCCGCGCCCGCCGCGCCCGCCGCCGACCAGGGCTCCTGGTGC
CTGTGCCAGCTCAATGGGGCCGCCGACCTCGAAGTTTCCGAAGCTGACATCATCTCTACC
GTTGAGTTCAACCACACGGGAGAGCTGCTGGCCACAGGTGACAAGGGCGGCCGGGTCGTC
ATCTTCCAGCGGGAACCAGAGAGTAAAAATGCGCCCCACAGCCAGGGCGAATACGACGTG
TACAGCACTTTCCAGAGCCACGAGCCGGAGTTTGACTATCTCAAGAGCCTGGAGATAGAG
GAGAAGATCAACAAGATCAAGTGGCTCCCACAGCAGAACGCCGCCCACTCACTCCTGTCC
ACCAACGATAAAACTATCAAATTATGGGAAGATTACCGAACGAGATAAAAAGGCCCGAAG
GATACAACCTGAAGGATGAAGAGGGGAAACTTAAGGACCCTGTCCACGGTGACGTCACTG
GAGGTGCCAGTGCTGAACCCCATGGATCTGATGGGGGAAGTGAGCCCTCGGAGAACCTTG
CCAATGGCCCACCTACCCATTAACTCATTTCCGGAACAGGGACTGGAAAACTACTTTTGG
GGGAGACCTGGCGTTAACCTTTGGCCCTGGCCTTACCAGAGGACTTTAAATTGGGACATT
AACCGGCAAAATGGGGGCCTTCGGGGGTACCAGAATT
>OriGene 3' read for NM_181876 unedited
ACTGGGGCAGGGGCTCACAGGGCATGCCACCCGGGCACTGTTCAGGAAACAGCTATGACC
GCGGCCGCAATCTAGAGTTTTTTTTTTTTTTTTTGATGTAACATTTGTTATTTTATTGGA
AAAAGCTGGTATTAACATATTTATAGTTTTATTCAACAATTGGGTAATTTGTGAGACACC
ATATAAAAAAAGAATGCACCTATGAGTTACAGAGGCCAAACTGATCAGGGCTGACAACTT
GACCACCATGTNTCCCACACCACCAGGCCGCCCACAAANNCGGAGAGAAGCTGCGGCCCA
AAAGAGAANNAAANNNAAAAAAGCGAAAANCAGCCACCCAACCAAAAAAAGNCCCCCACA
CGCGCCATTTTTAAAAANTNANAAAAAATTTTCTACAAAAGAACAACGACCTAATTNCCG
NGCAACCCCGAACAAAAGAACAAAAAACAGCATAACTAAACCAGCAACCTTCCAACGTAA
AATACGCATACGTCTGAAATGGACAATCTTTCCATGAGAAGGCCCGACCCCCAATAGAAA
AGAAGCAAGAAAAAGCTCCACCGCAAATAACACCACACACACTCCCCACTCTAAAACAAA
AACCAAATACAAAACCCATACACATAAAAATGACCACAAATCCAAATCACATACCAAATA
ACCCGCACAGCAGTTTATATGGAGCTATCGCCTACAAAGCAAAGACGCCCACGTAAACAT
AAAACAAAAAGATCCCAAAAGAGAAAACAAAAGTAAAACAAAAAAGGACTAACACGCCAA
AAAAGACACACCACAAGAAAGCAAAATGTAATACAACAGCAAAAAAAAACAAAA
Restriction Sites NotI-NotI     
ACCN NM_181876
Insert Size 4100 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_181876.1, NP_870991.1
RefSeq Size 4087 bp
RefSeq ORF 1344 bp
Locus ID 5522
Cytogenetics 4p16.1
Protein Families Druggable Genome, Phosphatase
Protein Pathways Tight junction
Gene Summary 'The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a gamma isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (2) contains an alternate 5' terminal exon, and it thus differs in the 5' UTR and 5' coding region, compared to variant 1. The encoded isoform (b) has a distinct N-terminus and is the same length as isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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