Neurokinin A Receptor (TACR2) (NM_001057) Human Tagged ORF Clone

CAT#: RG214272

TACR2 (GFP-tagged) - Human tachykinin receptor 2 (TACR2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TACR2
• Synonyms: NK2R; NKNAR; SKR; TAC2R
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214272 representing NM_001057
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGACCTGTGACATTGTGACTGAAGCCAATATCTCATCTGGCCCTGAGAGCAACACCACGGGCATCA
  CAGCCTTCTCCATGCCCAGCTGGCAACTGGCACTGTGGGCCACAGCCTACCTGGCCCTGGTGCTGGTGGC
  CGTGACGGGTAATGCCATCGTCATCTGGATCATCCTGGCCCATCGGAGGATGCGCACAGTCACCAACTAC
  TTCATCGTCAATCTGGCGCTGGCTGACCTCTGCATGGCTGCCTTCAATGCCGCCTTCAACTTTGTCTATG
  CCAGCCACAACATCTGGTACTTTGGCCGTGCCTTCTGCTACTTCCAGAACCTCTTCCCCATCACAGCCAT
  GTTTGTCAGCATCTACTCCATGACCGCCATTGCTGCCGACAGGTACATGGCCATCGTCCACCCCTTCCAG
  CCTCGGCTTTCAGCTCCCAGCACCAAGGCGGTTATTGCTGGCATCTGGCTGGTGGCTCTCGCCCTGGCCT
  CCCCTCAGTGCTTCTACTCCACCGTCACCATGGACCAGGGTGCCACCAAGTGCGTGGTGGCCTGGCCCGA
  AGACAGCGGGGGCAAGACGCTCCTCCTGTACCACCTCGTGGTGATCGCCCTCATCTACTTCCTGCCGCTC
  GCGGTGATGTTTGTAGCCTACAGCGTCATCGGCCTCACGCTCTGGAGGCGCGCAGTGCCCGAACATCAGG
  CGCACGGTGCCAACCTGCGCCACCTGCAGGCCAAGAAGAAGTTTGTGAAGACCATGGTGCTGGTGGTGCT
  GACGTTTGCCATCTGCTGGCTGCCCTACCACCTCTACTTCATCCTGGGCAGCTTCCAGGAGGACATCTAC
  TGCCACAAGTTCATCCAGCAAGTCTACCTGGCACTCTTCTGGTTGGCCATGAGCTCTACCATGTACAATC
  CCATCATCTACTGCTGTCTCAACCACAGGTTTCGCTCTGGATTCCGGCTTGCCTTCCGCTGCTGCCCATG
  GGTCACACCCACCAAGGAAGATAAGCTCGAGCTGACTCCCACGACCTCCCTCTCCACGAGAGTCAACAGG
  TGTCACACTAAGGAGACTTTGTTCATGGCTGGGGACACAGCCCCCTCCGAGGCTACCAGTGGGGAGGCGG
  GGCGTCCCCAGGATGGATCAGGGCTATGGTTTGGGTATGGTTTGCTTGCCCCCACCAAAACTCATGTTGA
  AATT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214272 representing NM_001057
  Red=Cloning site Green=Tags(s)
  
  MGTCDIVTEANISSGPESNTTGITAFSMPSWQLALWATAYLALVLVAVTGNAIVIWIILAHRRMRTVTNY
  FIVNLALADLCMAAFNAAFNFVYASHNIWYFGRAFCYFQNLFPITAMFVSIYSMTAIAADRYMAIVHPFQ
  PRLSAPSTKAVIAGIWLVALALASPQCFYSTVTMDQGATKCVVAWPEDSGGKTLLLYHLVVIALIYFLPL
  AVMFVAYSVIGLTLWRRAVPEHQAHGANLRHLQAKKKFVKTMVLVVLTFAICWLPYHLYFILGSFQEDIY
  CHKFIQQVYLALFWLAMSSTMYNPIIYCCLNHRFRSGFRLAFRCCPWVTPTKEDKLELTPTTSLSTRVNR
  CHTKETLFMAGDTAPSEATSGEAGRPQDGSGLWFGYGLLAPTKTHVEI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001057
• ORF Size: 1194 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001057.1, NP_001048.1
• RefSeq Size: 1197 bp
• RefSeq ORF: 1197 bp
• Locus ID: 6865
• Cytogenetics: 10q22.1
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction
• Gene Summary: 'This gene belongs to a family of genes that function as receptors for tachykinins. Receptor affinities are specified by variations in the 5'-end of the sequence. The receptors belonging to this family are characterized by interactions with G proteins and 7 hydrophobic transmembrane regions. This gene encodes the receptor for the tachykinin neuropeptide substance K, also referred to as neurokinin A. [provided by RefSeq, Jul 2008]'