SRGAP3 (NM_014850) Human Tagged ORF Clone

CAT#: RG214288

SRGAP3 (GFP-tagged) - Human SLIT-ROBO Rho GTPase activating protein 3 (SRGAP3), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SRGAP3
• Synonyms: ARHGAP14; MEGAP; SRGAP2; WRP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214288 representing NM_014850
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCATCTCAAACTAAGTTCAAGAAAGACAAAGAGATCATTGCTGAATATGAAGCCCAAATAAAAGAGA
  TCCGCACGCAGCTGGTGGAGCAGTTCAAATGTCTGGAGCAGCAATCAGAGTCGCGACTGCAGCTGCTTCA
  AGACCTCCAGGAGTTTTTCCGCCGGAAAGCTGAGATTGAGCTCGAGTACTCCCGCAGCCTGGAGAAGCTG
  GCTGAGCGCTTCTCCTCCAAAATCCGCAGCTCCCGGGAGCACCAGTTCAAGAAGGACCAGTACCTCCTCT
  CGCCTGTGAACTGTTGGTATCTGGTTCTGCATCAGACCCGGCGGGAGAGCCGAGACCATGCCACCCTCAA
  TGACATCTTCATGAACAATGTCATCGTCCGCCTCTCCCAGATCAGTGAGGATGTCATCAGACTCTTCAAA
  AAGAGCAAGGAGATTGGCCTGCAGATGCACGAGGAGCTCCTGAAGGTGACCAATGAGCTCTACACAGTCA
  TGAAAACCTACCACATGTACCATGCAGAGAGCATCAGTGCGGAAAGCAAGCTGAAGGAGGCTGAGAAGCA
  GGAGGAGAAGCAGTTCAATAAGTCAGGAGACCTCAGCATGAACCTGCTCCGGCACGAGGACCGGCCCCAG
  CGCCGCAGCTCTGTGAAGAAGATTGAGAAGATGAAGGAGAAGAGGCAGGCCAAGTACTCTGAGAACAAGC
  TGAAATGCACAAAGGCCCGGAATGACTACTTGCTCAATCTGGCAGCCACCAACGCAGCTATAAGCAAATA
  CTACATCCATGATGTCTCTGATCTGATCGATTGCTGTGATTTGGGCTTCCATGCCAGCCTGGCCCGCACC
  TTCCGGACCTATCTCTCAGCTGAATACAACCTGGAGACCTCTCGCCACGAAGGGCTGGATGTCATTGAGA
  ATGCAGTGGACAACCTGGATTCCCGAAGTGACAAGCACACAGTCATGGACATGTGCAATCAAGTCTTCTG
  CCCTCCACTCAAGTTCGAGTTCCAGCCCCACATGGGGGATGAGGTCTGCCAGGTCAGCGCTCAGCAGCCC
  GTCCAGACAGAACTGCTCATGCGTTATCACCAGCTGCAGTCCAGACTGGCCACCCTCAAGATAGAGAATG
  AGGAGGTTAGGAAAACCCTGGATGCCACCATGCAGACATTACAGGACATGCTGACTGTGGAGGACTTTGA
  TGTCTCCGATGCCTTCCAACACAGTCGATCGACAGAGTCCGTCAAGTCGGCTGCCTCTGAGACCTACATG
  AGCAAGATCAACATTGCCAAGAGGAGAGCCAACCAGCAGGAAACAGAAATGTTTTATTTTACAAAATTTA
  AAGAGTATGTGAATGGCAGTAACCTCATCACCAAGCTGCAGGCCAAGCACGATTTACTCAAGCAGACCCT
  GGGCGAAGGGGAAAGAGCAGAATGCGGCACCACCAGGCCCCCCTGTCTTCCCCCTAAACCACAGAAAATG
  AGGAGACCTAGGCCTCTCTCAGTGTATAGCCATAAACTCTTTAACGGCAGTATGGAAGCATTCATTAAGG
  ATTCAGGACAAGCTATACCGCTTGTAGTCGAGAGCTGCATCCGTTACATCAATTTATATGGACTCCAGCA
  GCAGGGGATCTTCAGAGTGCCAGGATCTCAGGTGGAAGTCAATGACATCAAAAATTCCTTTGAGAGAGGT
  GAAGACCCCCTTGTGGACGATCAAAATGAACGAGATATCAATTCAGTCGCTGGTGTTTTAAAACTGTATT
  TCCGAGGACTGGAAAACCCACTCTTTCCTAAGGAAAGGTTTCAAGATTTGATATCTACTATTAAACTGGA
  GAACCCAGCCGAGAGGGTGCACCAGATCCAACAAATCCTCGTCACCCTTCCCCGCGTGGTCATTGTGGTC
  ATGAGATACCTCTTCGCTTTCCTCAACCACCTCTCCCAGTATAGCGACGAGAACATGATGGATCCCTACA
  ACCTGGCCATCTGCTTCGGGCCTACCCTCATGCACATCCCTGATGGGCAGGACCCTGTGTCCTGCCAGGC
  ACACATCAATGAAGTCATCAAAACCATCATCATCCATCATGAAGCCATCTTCCCCAGCCCCCGGGAGCTA
  GAGGGACCTGTGTATGAAAAATGCATGGCTGGAGGGGAAGAATATTGTGACAGCCCACACAGCGAGCCAG
  GGGCCATCGATGAAGTTGACCATGACAATGGCACTGAGCCTCATACCAGCGATGAAGAAGTGGAGCAGAT
  CGAGGCTATTGCCAAGTTTGACTACATGGGGCGGTCCCCGCGTGAGCTATCCTTCAAGAAGGGGGCCTCG
  CTGCTCCTGTACCACCGCGCCTCGGAGGACTGGTGGGAGGGCCGGCACAACGGCGTGGATGGACTCATCC
  CCCATCAGTACATAGTTGTACAGGACATGGATGATGCCTTCTCCGACAGCCTGAGCCAGAAGGCTGACAG
  CGAGGCCAGCAGTGGGCCATTGCTGGATGACAAGGCCTCTTCCAAAAACGACCTCCAGTCCCCCACGGAG
  CACATCTCGGATTACGGCTTTGGGGGGGTGATGGGCCGAGTGCGGTTACGATCTGATGGAGCAGCCATCC
  CCAGACGCCGAAGCGGGGGCGACACACACAGCCCGCCCCGGGGCCTGGGCCCCAGCATAGACACACCACC
  CCGGGCTGCTGCCTGCCCCAGCAGCCCCCACAAAATCCCCCTCACCCGGGGGAGGATCGAGAGCCCTGAG
  AAGCGGAGGATGGCGACGTTCGGGAGCGCTGGCAGCATCAACTACCCTGACAAGAAGGCGCTCTCCGAAG
  GGCACTCGATGAGGTCGACCTGCGGTTCCACCAGGCACAGCAGCCTAGGGGACCACAAGTCCCTGGAGGC
  CGAGGCCCTGGCAGAAGACATCGAGAAGACCATGAGCACGGCTCTGCACGAGTTGCGGGAACTCGAGAGG
  CAGAACACGGTCAAGCAGGCGCCAGATGTGGTGCTGGACACCCTGGAGCCCCTGAAGAACCCGCCAGGCC
  CCGTCAGCTCGGAGCCCGCCAGTCCCCTTCACACCATCGTCATCCGCGACCCCGATGCCGCCATGCGCCG
  CAGCAGCAGCTCCTCCACCGAGATGATGACCACCTTCAAGCCAGCCCTGTCCGCCCGCCTGGCTGGCGCC
  CAGCTCCGCCCGCCCCCCATGCGGCCCGTGCGGCCGGTGGTCCAGCACCGGTCCAGCAGCAGCAGCAGCT
  CGGGCGTGGGCAGCCCGGCCGTGACGCCCACCGAGAAGATGTTCCCCAACAGCTCAGCGGACAAGTCGGG
  CACCATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214288 representing NM_014850
  Red=Cloning site Green=Tags(s)
  
  MSSQTKFKKDKEIIAEYEAQIKEIRTQLVEQFKCLEQQSESRLQLLQDLQEFFRRKAEIELEYSRSLEKL
  AERFSSKIRSSREHQFKKDQYLLSPVNCWYLVLHQTRRESRDHATLNDIFMNNVIVRLSQISEDVIRLFK
  KSKEIGLQMHEELLKVTNELYTVMKTYHMYHAESISAESKLKEAEKQEEKQFNKSGDLSMNLLRHEDRPQ
  RRSSVKKIEKMKEKRQAKYSENKLKCTKARNDYLLNLAATNAAISKYYIHDVSDLIDCCDLGFHASLART
  FRTYLSAEYNLETSRHEGLDVIENAVDNLDSRSDKHTVMDMCNQVFCPPLKFEFQPHMGDEVCQVSAQQP
  VQTELLMRYHQLQSRLATLKIENEEVRKTLDATMQTLQDMLTVEDFDVSDAFQHSRSTESVKSAASETYM
  SKINIAKRRANQQETEMFYFTKFKEYVNGSNLITKLQAKHDLLKQTLGEGERAECGTTRPPCLPPKPQKM
  RRPRPLSVYSHKLFNGSMEAFIKDSGQAIPLVVESCIRYINLYGLQQQGIFRVPGSQVEVNDIKNSFERG
  EDPLVDDQNERDINSVAGVLKLYFRGLENPLFPKERFQDLISTIKLENPAERVHQIQQILVTLPRVVIVV
  MRYLFAFLNHLSQYSDENMMDPYNLAICFGPTLMHIPDGQDPVSCQAHINEVIKTIIIHHEAIFPSPREL
  EGPVYEKCMAGGEEYCDSPHSEPGAIDEVDHDNGTEPHTSDEEVEQIEAIAKFDYMGRSPRELSFKKGAS
  LLLYHRASEDWWEGRHNGVDGLIPHQYIVVQDMDDAFSDSLSQKADSEASSGPLLDDKASSKNDLQSPTE
  HISDYGFGGVMGRVRLRSDGAAIPRRRSGGDTHSPPRGLGPSIDTPPRAAACPSSPHKIPLTRGRIESPE
  KRRMATFGSAGSINYPDKKALSEGHSMRSTCGSTRHSSLGDHKSLEAEALAEDIEKTMSTALHELRELER
  QNTVKQAPDVVLDTLEPLKNPPGPVSSEPASPLHTIVIRDPDAAMRRSSSSSTEMMTTFKPALSARLAGA
  QLRPPPMRPVRPVVQHRSSSSSSSGVGSPAVTPTEKMFPNSSADKSGTM
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014850
• ORF Size: 3297 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_014850.2, NP_055665.1
• RefSeq Size: 8901
• RefSeq ORF: 3300
• Locus ID: 9901
• Domains: RhoGAP, FCH, SH3
• Protein Pathways: Axon guidance