CDC25A (NM_201567) Human Tagged ORF Clone

CAT#: RG214325

CDC25A (GFP-tagged) - Human cell division cycle 25 homolog A (S. pombe) (CDC25A), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CDC25A
• Synonyms: CDC25A2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214325 representing NM_201567
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACTGGGCCCGGAGCCCCCGCACCGCCGCCGCCTGCTCTTCGCCTGCAGCCCCCCTCCCGCGTCGC
  AGCCCGTCGTGAAGGCGCTATTTGGCGCTTCAGCCGCCGGGGGACTGTCGCCTGTCACCAACCTGACCGT
  CACTATGGACCAGCTGCAGGGTCTGGGCAGTGATTATGAGCAACCACTGGAGGTGAAGAACAACAGTAAT
  CTGCAGAGAATGGGCTCCTCCGAGTCAACAGATTCAGGTTTCTGTCTAGATTCTCCTGGGCCATTGGACA
  GTAAAGAAAACCTTGAAAATCCTATGAGAAGAATACATTCCCTACCTCAGAAGCTGTTGGGATGTAGTCC
  AGCTCTGAAGAGGAGCCATTCTGATTCTCTTGACCATGACATCTTTCAGCTCATCGACCCAGATGAGAAC
  AAGGAAAATCTTTCCTCAAATGAAAGAGATAGCAGTGAACCAGGGAATTTCATTCCTCTTTTTACACCCC
  AGTCACCTGTGACAGCCACTTTGTCTGATGAGGATGATGGCTTCGTGGACCTTCTCGATGGAGAGAATCT
  GAAGAATGAGGAGGAGACCCCCTCGTGCATGGCAAGCCTCTGGACAGCTCCTCTCGTCATGAGAACTACA
  AACCTTGACAACCGATGCAAGCTGTTTGACTCCCCTTCCCTGTGTAGCTCCAGCACTCGGTCAGTGTTGA
  AGAGACCAGAACGATCTCAAGAGGAGTCTCCACCTGGAAGTACAAAGAGGAGGAAGAGCATGTCTGGGGC
  CAGCCCCAAAGAGTCAACTAATCCAGAGAAGGCCCATGAGACTCTTCATCAGTCTTTATCCCTGGCATCT
  TCCCCCAAAGGAACCATTGAGAACATTTTGGACAATGACCCAAGGGACCTTATAGGAGACTTCTCCAAGG
  GTTATCTCTTTCATACAGTTGCTGGGAAACATCAGGATTTAAAATACATCTCTCCAGAAATTATGGCATC
  TGTTTTGAATGGCAAGTTTGCCAACCTCATTAAAGAGTTTGTTATCATCGACTGTCGATACCCATATGAA
  TACGAGGGAGGCCACATCAAGGGTGCAGTGAACTTGCACATGGAAGAAGAGGTTGAAGACTTCTTATTGA
  AGAAGCCCATTGTACCTACTGATGGCAAGCGTGTCATTGTTGTGTTTCACTGCGAGTTTTCTTCTGAGAG
  AGGTCCCCGCATGTGCCGGTATGTGAGAGAGAGAGATCGCCTGGGTAATGAATACCCCAAACTCCACTAC
  CCTGAGCTGTATGTCCTGAAGGGGGGATACAAGGAGTTCTTTATGAAATGCCAGTCTTACTGTGAGCCCC
  CTAGCTACCGGCCCATGCACCACGAGGACTTTAAAGAAGACCTGAAGAAGTTCCGCACCAAGAGCCGGAC
  CTGGGCAGGGGAGAAGAGCAAGAGGGAGATGTACAGTCGTCTGAAGAAGCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214325 representing NM_201567
  Red=Cloning site Green=Tags(s)
  
  MELGPEPPHRRRLLFACSPPPASQPVVKALFGASAAGGLSPVTNLTVTMDQLQGLGSDYEQPLEVKNNSN
  LQRMGSSESTDSGFCLDSPGPLDSKENLENPMRRIHSLPQKLLGCSPALKRSHSDSLDHDIFQLIDPDEN
  KENLSSNERDSSEPGNFIPLFTPQSPVTATLSDEDDGFVDLLDGENLKNEEETPSCMASLWTAPLVMRTT
  NLDNRCKLFDSPSLCSSSTRSVLKRPERSQEESPPGSTKRRKSMSGASPKESTNPEKAHETLHQSLSLAS
  SPKGTIENILDNDPRDLIGDFSKGYLFHTVAGKHQDLKYISPEIMASVLNGKFANLIKEFVIIDCRYPYE
  YEGGHIKGAVNLHMEEEVEDFLLKKPIVPTDGKRVIVVFHCEFSSERGPRMCRYVRERDRLGNEYPKLHY
  PELYVLKGGYKEFFMKCQSYCEPPSYRPMHHEDFKEDLKKFRTKSRTWAGEKSKREMYSRLKKL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_201567
• ORF Size: 1452 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_201567.1, NP_963861.1
• RefSeq Size: 3597
• RefSeq ORF: 1455
• Locus ID: 993
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Cell cycle, Progesterone-mediated oocyte maturation
• Gene Summary: CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]