GPR115 (ADGRF4) (NM_153838) Human Tagged ORF Clone

CAT#: RG214357

GPR115 (GFP-tagged) - Human G protein-coupled receptor 115 (GPR115)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ADGRF4
• Synonyms: GPR115; PGR18
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214357 representing NM_153838
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAATGAAGTCCCAGGCAACCATGATTTGCTGCTTAGTGTTCTTTCTGTCCACAGAATGTTCCCACT
  ATAGATCCAAGATTCACCTAAAAGCTGGAGATAAACTTCAAAGCCCTGAAGGGAAACCCAAGACTGGAAG
  GATCCAAGAGAAATGCGAAGGACCTTGTATTTCTTCTTCCAACTGCAGCCAGCCCTGTGCTAAGGACTTT
  CATGGAGAAATAGGATTTACATGTAATCAAAAAAAGTGGCAAAAATCAGCTGAAACATGTACAAGCCTTT
  CTGTGGAAAAACTCTTTAAGGACTCAACTGGTGCATCTCGCCTTTCTGTAGCAGCACCATCTATACCTCT
  GCATATTCTAGACTTTCGAGCTCCAGAGACCATTGAGAGTGTAGCTCAAGGAATCCGTAAGAACTGCCCC
  TTTGATTATGCCTGCATCACTGACATGGTGAAATCATCAGAAACAACATCTGGAAATATTGCATTTATAG
  TGGAGTTATTAAAAAATATTTCTACAGACTTGTCTGATAATGTTACTCGAGAGAAAATGAAGAGCTATAG
  TGAAGTGGCCAACCACATCCTCGACACAGCAGCCATTTCAAACTGGGCTTTCATTCCCAACAAAAATGCC
  AGCTCGGATTTGTTGCAGTCAGTGAATTTGTTTGCCAGACAACTCCACATCCACAATAATTCTGAGAACA
  TTGTGAATGAACTCTTCATTCAGACAAAAGGGTTTCACATCAACCATAATACCTCAGAGAAAAGCCTCAA
  TTTCTCCATGAGCATGAACAATACCACAGAAGATATCTTAGGAATGGTACAGATTCCCAGGCAAGAGCTA
  AGGAAGCTGTGGCCAAATGCATCCCAAGCCATTAGCATAGCTTTCCCAACCTTGGGGGCTATCCTGAGAG
  AAGCCCACTTGCAAAATGTGAGTCTTCCCAGACAGGTAAATGGTCTGGTGCTATCAGTGGTTTTACCAGA
  AAGGTTGCAAGAAATCATACTCACCTTCGAAAAGATCAATAAAACCCGCAATGCCAGAGCCCAGTGTGTT
  GGCTGGCACTCCAAGAAAAGGAGATGGGATGAGAAAGCGTGCCAAATGATGTTGGATATCAGGAACGAAG
  TGAAATGCCGCTGTAACTACACCAGTGTGGTGATGTCTTTTTCCATTCTCATGTCCTCCAAATCGATGAC
  CGACAAAGTTCTGGACTACATCACCTGCATTGGGCTCAGCGTCTCAATCCTAAGCTTGGTTCTTTGCCTG
  ATCATTGAAGCCACAGTGTGGTCCCGGGTGGTTGTGACGGAGATATCATACATGCGTCACGTGTGCATCG
  TGAATATAGCAGTGTCCCTTCTGACTGCCAATGTGTGGTTTATCATAGGCTCTCACTTTAACATTAAGGC
  CCAGGACTACAACATGTGTGTTGCAGTGACATTTTTCAGCCACTTTTTCTACCTCTCTCTGTTTTTCTGG
  ATGCTCTTCAAAGCATTGCTCATCATTTATGGAATATTGGTCATTTTCCGTAGGATGATGAAGTCCCGAA
  TGATGGTCATTGGCTTTGCCATTGGCTATGGGTGCCCATTGATCATTGCTGTCACTACAGTTGCTATCAC
  AGAGCCAGAGAACGGCTACATGAGACCTGAGGCCTGTTGGCTTAACTGGGACAATACCAAAGCCCTTTTA
  GCATTTGCCATCCCGGCGTTCGTCATTGTGGCTGTAAATCTGATTGTGGTTTTGGTTGTTGCTGTCAACA
  CTCAGAGGCCCTCTATTGGCAGTTCCAAGTCTCAGGATGTGGTCATAATTATGAGGATCAGCAAAAATGT
  TGCCATCCTCACTCCACTGCTGGGACTGACCTGGGGTTTTGGAATAGCCACTCTCATAGAAGGCACTTCC
  TTGACGTTCCATATAATTTTTGCCTTGCTCAATGCTTTCCAGGGTTTTTTCATCCTGCTGTTTGGAACCA
  TTATGGATCACAAGATAAGAGATGCTTTGAGGATGAGGATGTCTTCACTGAAGGGGAAATCGAGGGCAGC
  TGAGAATGCATCACTAGGCCCAACCAATGGATCTAAATTAATGAATCGTCAAGGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214357 representing NM_153838
  Red=Cloning site Green=Tags(s)
  
  MKMKSQATMICCLVFFLSTECSHYRSKIHLKAGDKLQSPEGKPKTGRIQEKCEGPCISSSNCSQPCAKDF
  HGEIGFTCNQKKWQKSAETCTSLSVEKLFKDSTGASRLSVAAPSIPLHILDFRAPETIESVAQGIRKNCP
  FDYACITDMVKSSETTSGNIAFIVELLKNISTDLSDNVTREKMKSYSEVANHILDTAAISNWAFIPNKNA
  SSDLLQSVNLFARQLHIHNNSENIVNELFIQTKGFHINHNTSEKSLNFSMSMNNTTEDILGMVQIPRQEL
  RKLWPNASQAISIAFPTLGAILREAHLQNVSLPRQVNGLVLSVVLPERLQEIILTFEKINKTRNARAQCV
  GWHSKKRRWDEKACQMMLDIRNEVKCRCNYTSVVMSFSILMSSKSMTDKVLDYITCIGLSVSILSLVLCL
  IIEATVWSRVVVTEISYMRHVCIVNIAVSLLTANVWFIIGSHFNIKAQDYNMCVAVTFFSHFFYLSLFFW
  MLFKALLIIYGILVIFRRMMKSRMMVIGFAIGYGCPLIIAVTTVAITEPENGYMRPEACWLNWDNTKALL
  AFAIPAFVIVAVNLIVVLVVAVNTQRPSIGSSKSQDVVIIMRISKNVAILTPLLGLTWGFGIATLIEGTS
  LTFHIIFALLNAFQGFFILLFGTIMDHKIRDALRMRMSSLKGKSRAAENASLGPTNGSKLMNRQG
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153838
• ORF Size: 2085 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_153838.2, NP_722580.2
• RefSeq Size: 2574
• RefSeq ORF: 2088
• Locus ID: 221393
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: Sequence analysis of this gene suggests that it is encodes a member of the superfamily of G protein-couple receptors. G protein-coupled receptors typically contain seven hydrophobic transmembrane domains, interact with guanine nucleotide binding regulatory proteins, and detect molecules outside the cell and act to transduce these signals into intracellular responses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2016]