PDE5A (NM_001083) Human Tagged ORF Clone
CAT#: RG214535
- TrueORF®
PDE5A (GFP-tagged) - Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 1
"NM_001083" in other vectors (6)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | PDE5A |
Synonyms | CGB-PDE; CN5A; PDE5 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG214535 representing NM_001083
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGGGCCGGCCCCAGCTTCGGGCAGCAGCGACAGCAGCAGCAGCCCCAGCAGCAGAAGCAGCAGC AGAGGGATCAGGACTCGGTCGAAGCATGGCTGGACGATCACTGGGACTTTACCTTCTCATACTTTGTTAG AAAAGCCACCAGAGAAATGGTCAATGCATGGTTTGCTGAGAGAGTTCACACCATCCCTGTGTGCAAGGAA GGTATCAGAGGCCACACCGAATCTTGCTCTTGTCCCTTGCAGCAGAGTCCTCGTGCAGATAACAGTGCCC CTGGAACACCAACCAGGAAAATCTCTGCCTCTGAATTTGACCGGCCTCTTAGACCCATTGTTGTCAAGGA TTCTGAGGGAACTGTGAGCTTCCTCTCTGACTCAGAAAAGAAGGAACAGATGCCTCTAACCCCTCCAAGG TTTGATCATGATGAAGGGGACCAGTGCTCAAGACTCTTGGAATTAGTGAAGGATATTTCTAGTCATTTGG ATGTCACAGCCTTATGTCACAAAATTTTCTTGCATATCCATGGACTGATATCTGCTGACCGCTATTCCCT GTTCCTTGTCTGTGAAGACAGCTCCAATGACAAGTTTCTTATCAGCCGCCTCTTTGATGTTGCTGAAGGT TCAACACTGGAAGAAGTTTCAAATAACTGTATCCGCTTAGAATGGAACAAAGGCATTGTGGGACATGTGG CAGCGCTTGGTGAGCCCTTGAACATCAAAGATGCATATGAGGATCCTCGGTTCAATGCAGAAGTTGACCA AATTACAGGCTACAAGACACAAAGCATTCTTTGTATGCCAATTAAGAATCATAGGGAAGAGGTTGTTGGT GTAGCCCAGGCCATCAACAAGAAATCAGGAAACGGTGGGACATTTACTGAAAAAGATGAAAAGGACTTTG CTGCTTATTTGGCATTTTGTGGTATTGTTCTTCATAATGCTCAGCTCTATGAGACTTCACTGCTGGAGAA CAAGAGAAATCAGGTGCTGCTTGACCTTGCTAGTTTAATTTTTGAAGAACAACAATCATTAGAAGTAATT TTGAAGAAAATAGCTGCCACTATTATCTCTTTCATGCAAGTGCAGAAATGCACCATTTTCATAGTGGATG AAGATTGCTCCGATTCTTTTTCTAGTGTGTTTCACATGGAGTGTGAGGAATTAGAAAAATCATCTGATAC ATTAACAAGGGAACATGATGCAAACAAAATCAATTACATGTATGCTCAGTATGTCAAAAATACTATGGAA CCACTTAATATCCCAGATGTCAGTAAGGATAAAAGATTTCCCTGGACAACTGAAAATACAGGAAATGTAA ACCAGCAGTGCATTAGAAGTTTGCTTTGTACACCTATAAAAAATGGAAAGAAGAATAAAGTTATAGGGGT TTGCCAACTTGTTAATAAGATGGAGGAGAATACTGGCAAGGTTAAGCCTTTCAACCGAAATGACGAACAG TTTCTGGAAGCTTTTGTCATCTTTTGTGGCTTGGGGATCCAGAACACGCAGATGTATGAAGCAGTGGAGA GAGCCATGGCCAAGCAAATGGTCACATTGGAGGTTCTGTCGTATCATGCTTCAGCAGCAGAGGAAGAAAC AAGAGAGCTACAGTCGTTAGCGGCTGCTGTGGTGCCATCTGCCCAGACCCTTAAAATTACTGACTTTAGC TTCAGTGACTTTGAGCTGTCTGATCTGGAAACAGCACTGTGTACAATTCGGATGTTTACTGACCTCAACC TTGTGCAGAACTTCCAGATGAAACATGAGGTTCTTTGCAGATGGATTTTAAGTGTTAAGAAGAATTATCG GAAGAATGTTGCCTATCATAATTGGAGACATGCCTTTAATACAGCTCAGTGCATGTTTGCTGCTCTAAAA GCAGGCAAAATTCAGAACAAGCTGACTGACCTGGAGATACTTGCATTGCTGATTGCTGCACTAAGCCACG ATTTGGATCACCGTGGTGTGAATAACTCTTACATACAGCGAAGTGAACATCCACTTGCCCAGCTTTACTG CCATTCAATCATGGAACACCATCATTTTGACCAGTGCCTGATGATTCTTAATAGTCCAGGCAATCAGATT CTCAGTGGCCTCTCCATTGAAGAATATAAGACCACGTTGAAAATAATCAAGCAAGCTATTTTAGCTACAG ACCTAGCACTGTACATTAAGAGGCGAGGAGAATTTTTTGAACTTATAAGAAAAAATCAATTCAATTTGGA AGATCCTCATCAAAAGGAGTTGTTTTTGGCAATGCTGATGACAGCTTGTGATCTTTCTGCAATTACAAAA CCCTGGCCTATTCAACAACGGATAGCAGAACTTGTAGCAACTGAATTTTTTGATCAAGGAGACAGAGAGA GAAAAGAACTCAACATAGAACCCACTGATCTAATGAACAGGGAGAAGAAAAACAAAATCCCAAGTATGCA AGTTGGGTTCATAGATGCCATCTGCTTGCAACTGTATGAGGCCCTGACCCACGTGTCAGAGGACTGTTTC CCTTTGCTAGATGGCTGCAGAAAGAACAGGCAGAAATGGCAGGCCCTTGCAGAACAGCAGGAGAAGATGC TGATTAATGGGGAAAGCGGCCAGGCCAAGCGGAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214535 representing NM_001083
Red=Cloning site Green=Tags(s) MERAGPSFGQQRQQQQPQQQKQQQRDQDSVEAWLDDHWDFTFSYFVRKATREMVNAWFAERVHTIPVCKE GIRGHTESCSCPLQQSPRADNSAPGTPTRKISASEFDRPLRPIVVKDSEGTVSFLSDSEKKEQMPLTPPR FDHDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCEDSSNDKFLISRLFDVAEG STLEEVSNNCIRLEWNKGIVGHVAALGEPLNIKDAYEDPRFNAEVDQITGYKTQSILCMPIKNHREEVVG VAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVI LKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELEKSSDTLTREHDANKINYMYAQYVKNTME PLNIPDVSKDKRFPWTTENTGNVNQQCIRSLLCTPIKNGKKNKVIGVCQLVNKMEENTGKVKPFNRNDEQ FLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETRELQSLAAAVVPSAQTLKITDFS FSDFELSDLETALCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALK AGKIQNKLTDLEILALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQI LSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKNQFNLEDPHQKELFLAMLMTACDLSAITK PWPIQQRIAELVATEFFDQGDRERKELNIEPTDLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCF PLLDGCRKNRQKWQALAEQQEKMLINGESGQAKRN TRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001083 |
ORF Size | 2625 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Reference Data | |
RefSeq | NM_001083.3, NP_001074.2 |
RefSeq Size | 7005 |
RefSeq ORF | 2628 |
Locus ID | 8654 |
Domains | PDEase, GAF, HDc |
Protein Families | Druggable Genome |
Protein Pathways | Progesterone-mediated oocyte maturation, Purine metabolism |
Gene Summary | This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC123995 | PDE5A (untagged)-Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 1 |
USD 760.00 |
|
RC214535 | PDE5A (Myc-DDK-tagged)-Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 1 |
USD 420.00 |
|
RC214535L1 | Lenti-ORF clone of PDE5A (Myc-DDK-tagged)-Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 1 |
USD 620.00 |
|
RC214535L2 | Lenti-ORF clone of PDE5A (mGFP-tagged)-Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 1 |
USD 768.00 |
|
RC214535L3 | Lenti-ORF clone of PDE5A (Myc-DDK-tagged)-Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 1 |
USD 620.00 |
|
RC214535L4 | Lenti-ORF clone of PDE5A (mGFP-tagged)-Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 1 |
USD 768.00 |
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