PDE5A (NM_001083) Human Untagged Clone

CAT#: SC123995

PDE5A (untagged)-Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDE5A
• Synonyms: CGB-PDE; CN5A; PDE5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC123995 sequence for NM_001083 edited (data generated by NextGen Sequencing)
  ATGGAGCGGGCCGGCCCCAGCTTCGGGCAGCAGCGACAGCAGCAGCAGCCCCAGCAGCAG
  AAGCAGCAGCAGAGGGATCAGGACTCGGTCGAAGCATGGCTGGACGATCACTGGGACTTT
  ACCTTCTCATACTTTGTTAGAAAAGCCACCAGAGAAATGGTCAATGCATGGTTTGCTGAG
  AGAGTTCACACCATCCCTGTGTGCAAGGAAGGTATCAGAGGCCACACCGAATCTTGCTCT
  TGTCCCTTGCAGCAGAGTCCTCGTGCAGATAACAGTGTCCCTGGAACACCAACCAGGAAA
  ATCTCTGCCTCTGAATTTGACCGGCCTCTTAGACCCATTGTTGTCAAGGATTCTGAGGGA
  ACTGTGAGCTTCCTCTCTGACTCAGAAAAGAAGGAACAGATGCCTCTAACCCCTCCAAGG
  TTTGATCATGATGAAGGGGACCAGTGCTCAAGACTCTTGGAATTAGTGAAGGATATTTCT
  AGTCATTTGGATGTCACAGCCTTATGTCACAAAATTTTCTTGCATATCCATGGACTGATA
  TCTGCTGACCGCTATTCCCTGTTCCTTGTCTGTGAAGACAGCTCCAATGACAAGTTTCTT
  ATCAGCCGCCTCTTTGATGTTGCTGAAGGTTCAACACTGGAAGAAGTTTCAAATAACTGT
  ATCCGCTTAGAATGGAACAAAGGCATTGTGGGACATGTGGCAGCGCTTGGTGAGCCCTTG
  AACATCAAAGATGCATATGAGGATCCTCGGTTCAATGCAGAAGTTGACCAAATTACAGGC
  TACAAGACACAAAGCATTCTTTGTATGCCAATTAAGAATCATAGGGAAGAGGTTGTTGGT
  GTAGCCCAGGCCATCAACAAGAAATCAGGAAACGGTGGGACATTTACTGAAAAAGATGAA
  AAGGACTTTGCTGCTTATTTGGCATTTTGTGGTATTGTTCTTCATAATGCTCAGCTCTAT
  GAGACTTCACTGCTGGAGAACAAGAGAAATCAGGTGCTGCTTGACCTTGCTAGTTTAATT
  TTTGAAGAACAACAATCATTAGAAGTAATTTTGAAGAAAATAGCTGCCACTATTATCTCT
  TTCATGCAAGTGCAGAAATGCACCATTTTCATAGTGGATGAAGATTGCTCCGATTCTTTT
  TCTAGTGTGTTTCACATGGAGTGTGAGGAATTAGAAAAATCATCTGATACATTAACAAGG
  GAACATGATGCAAACAAAATCAATTACATGTATGCTCAGTAYGTCAAAAATACTATGGAA
  CCACTTAATATCCCAGATGTCAGTAAGGATAAAAGATTTCCCTGGACAACTGAAAATACA
  GGAAATGTAAACCAGCAGTGCATTAGAAGTTTGCTTTGTACACCTATAAAAAATGGAAAG
  AAGAATAAAGTTATAGGGGTTTGCCAACTTGTTAATAAGATGGAGGAGAATACTGGCAAG
  GTTAAGCCTTTCAACCGAAATGACGAACAGTTTCTGGAAGCTTTTGTCATCTTTTGTGGC
  TTGGGGATCCAGAACACGCAGATGTATGAAGCAGTGGAGAGAGCCATGGCCAAGCAAATG
  GTCACATTGGAGGTTCTGTCGTATCATGCTTCAGCAGCAGAGGAAGAAACAAGAGAGCTA
  CAGTCGTTAGCGGCTGCTGTGGTGCCATCTGCCCAGACCCTTAAAATTACTGACTTTAGC
  TTCAGTGACTTTGAGCTGTCTGATCTGGAAACAGCACTGTGTACAATTCGGATGTTTACT
  GACCTCAACCTTGTGCAGAACTTCCAGATGAAACATGAGGTTCTTTGCAGATGGATTTTA
  AGTGTTAAGAAGAATTATCGGAAGAATGTTGCCTATCATAATTGGAGACATGCCTTTAAT
  ACAGCTCAGTGCATGTTTGCTGCTCTAAAAGCAGGCAAAATTCAGAACAAGCTGACTGAC
  CTGGAGATACTTGCATTGCTGATTGCTGCACTAAGCCACGATTTGGATCACCGTGGTGTG
  AATAACTCTTACATACAGCGAAGTGAACATCCACTTGCCCAGCTTTACTGCCATTCAATC
  ATGGAACACCATCATTTTGACCAGTGCCTGATGATTCTTAATAGTCCAGGCAATCAGATT
  CTCAGTGGCCTCTCCATTGAAGAATATAAGACCACGTTGAAAATAATCAAGCAAGCTATT
  TTAGCTACAGACCTAGCACTGTACATTAAGAGGCGAGGAGAATTTTTTGAACTTATAAGA
  AAAAATCAATTCAATTTGGAAGATCCTCATCAAAAGGAGTTGTTTTTGGCAATGCTGATG
  ACAGCTTGTGATCTTTCTGCAATTACAAAACCCTGGCCTATTCAACAACGGATAGCAGAA
  CTTGTAGCAACTGAATTTTTTGATCAAGGAGACAGAGAGAGAAAAGAACTCAACATAGAA
  CCCACTGATCTAATGAACAGGGAGAAGAAAAACAAAATCCCAAGTATGCAAGTTGGGTTC
  ATAGATGCCATCTGCTTGCAACTGTATGAGGCCCTGACCCACGTGTCAGAGGACTGTTTC
  CCTTTGCTAGATGGCTGCAGAAAGAACAGGCAGAAATGGCAGGCCCTTGCAGAACAGCAG
  GAGAAGATGCTGATTAATGGGGAAAGCGGCCAGGCCAAGCGGAACTGA
  
  Clone variation with respect to NM_001083.3
  278 c=>t;1242 t=>y
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001083 unedited
  ACGAAGACTATAGGGCGGCCGCGAATTCGGCACGAGGCAGGACGAAGGGGGAGGGTCTCG
  AGGCCGAGTCCTGTTCTTCTGAGGGACGGACCCCAGCTGGGGTGGAAAAGCAGTACCAGA
  GAGCCTCCGAGGCGCGCGGTGCCAACCATGGAGCGGGCCGGCCCCAGCTTCGGGCAGCAG
  CGACAGCAGCAGCAGCCCCAGCAGCAGAAGCAGCAGCAGAGGGATCAGGACTCGGTCGAA
  GCATGGCTGGACGATCACTGGGACTTTACCTTCTCATACTTTGTTAGAAAAGCCACCAGA
  GAAATGGTCAATGCATGGTTTGCTGAGAGAGTTCACACCATCCCTGTGTGCAAGGAAGGT
  ATCAGAGGCCACACCGAATCTTGCTCTTGTCCCTTGCAGCAGAGTCCTCGTGCAGATAAC
  AGTGTCCCTGGAACACCAACCAGGAAAATCTCTGCCTCTGAATTTGACCGGCCTCTTAGA
  CCCATTGTTGTCAAGGATTCTGAGGGAACTGTGAGCTTCCTCTCTGACTCAGAAAAGAAG
  GAACAGATGCCTCTAACCCCTCCAAGGTTTGATCATGATGAAGGGGACCAGTGCTCAAGA
  CTCTTGGAATTAGTGAAGGATATTTCTAGTCATTTGGATGTCACAGCCTTATGTCACAAA
  ATTTTCTTGCATATCCATGGACTGATATCTGCTGACCGCTATTCCCTGGTTCTTGTCTGT
  GAAACAGCTCCATGACAGTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001083 unedited
  TCCGAGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGG
  AAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTACCTGAACAATCTTTTTATTTTTACAAAGCCTG
  GGAAAATGGGGAAACCCCTGGCCGGGGAAAAATTTGCCCAATGGAAAACCCCCTTGACCA
  TTTCATGACTTTTTACAGGAATCGGAGGGAAGTGTTCCTTTAAAGGGATTTTGTCATGAA
  AAAGTCATCTGAAATGACTCTCGTGATGGGGGAAAGGAAAGTAATGAAATCAACCTTCAT
  GGTTATTTAAAATGCCTTAAAAGCTAAGCCTAATATACATAGTAACCCCTTTTCCTAGTA
  TAAAAATAGTGCCAGAAACTATTTTTGTTCAAATTTTTTGAAAAGGTTTAAGAATCCCAA
  TTGTTTTTAACCGTGGCTAGTGATGCCTCATAAGGTTTTCTTAAACTGGAATATAAATTT
  ATATTTTCAACTATTTACATTGCTTTCAACAACCTTCATTAAAACATGTTTACACAGGCT
  TTTATAAGTATATGGAATGGAAAGGGGCTTTATACTTTTTCTTCCTCTCTGTGTTAACAG
  AGGGGCCAAACCTGCCCATTTACAGTCCAAAAACTGGTATTTTACTGGTTGAATGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001083
• ORF Size: 2628 bp
• Insert Size: 8000
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_001083.3, NP_001074.2
• RefSeq Size: 7005
• RefSeq ORF: 2628
• Locus ID: 8654
• Domains: PDEase, GAF, HDc
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• Gene Summary: This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1, also known as PDE5A1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.