AKR7A2 (NM_003689) Human Tagged ORF Clone

CAT#: RG214673

AKR7A2 (GFP-tagged) - Human aldo-keto reductase family 7, member A2 (aflatoxin aldehyde reductase) (AKR7A2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AKR7A2
• Synonyms: AFAR; AFAR1; AFB1-AR1; AKR7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214673 representing NM_003689
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAGTGCCGCGTCTCGCGTAGTCTCCCGCGCCGCCGTCCACTGCGCGCTTCGCTCTCCGCCGCCCG
  AGGCCCGCGCGCTCGCCATGTCCCGGCCACCGCCACCGCGGGTCGCCTCGGTGCTGGGCACCATGGAGAT
  GGGGCGCCGCATGGACGCGCCCGCCAGCGCCGCGGCCGTGCGCGCCTTTCTGGAGCGCGGCCACACCGAA
  CTGGACACGGCCTTCATGTACAGCGACGGCCAGTCCGAGACCATCCTGGGCGGCCTGGGGCTCGGGCTGG
  GCGGTGGCGACTGCAGAGTGAAAATTGCCACCAAGGCCAACCCTTGGGATGGAAAATCACTAAAGCCTGA
  CAGTGTCCGGTCCCAGCTGGAGACGTCATTGAAGAGGCTGCAGTGTCCCCAAGTGGACCTCTTCTACCTA
  CACGCACCTGACCACGGCACCCCGGTGGAAGAGACGCTGCATGCCTGCCAGCGGCTGCACCAGGAGGGCA
  AGTTCGTGGAGCTTGGCCTCTCCAACTATGCTAGCTGGGAAGTGGCCGAGATCTGTACCCTCTGCAAGAG
  CAATGGCTGGATCCTGCCCACTGTGTACCAGGGCATGTACAACGCCACCACCCGGCAGGTGGAAACGGAG
  CTCTTCCCCTGCCTCAGGCACTTTGGACTGAGGTTCTATGCCTACAACCCTCTGGCTGGGGGCCTGCTGA
  CTGGCAAGTACAAGTATGAGGACAAGGACGGGAAACAGCCTGTGGGCCGCTTCTTTGGGAATAGCTGGGC
  TGAGACCTACAGGAATCGCTTCTGGAAGGAGCACCACTTCGAGGCCATTGCGTTGGTGGAGAAGGCCCTG
  CAGGCCGCATATGGCGCCAGCGCCCCCAGTGTGACCTCGGCTGCCCTCCGGTGGATGTACCACCACTCAC
  AGCTGCAGGGTGCCCACGGGGACGCGGTCATCCTGGGCATGTCCAGCCTGGAGCAGCTGGAGCAGAACTT
  GGCAGCAACAGAGGAAGGGCCCCTGGAGCCGGCTGTCGTGGATGCCTTTAATCAAGCCTGGCATTTGGTT
  GCTCACGAATGTCCCAACTACTTCCGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214673 representing NM_003689
  Red=Cloning site Green=Tags(s)
  
  MLSAASRVVSRAAVHCALRSPPPEARALAMSRPPPPRVASVLGTMEMGRRMDAPASAAAVRAFLERGHTE
  LDTAFMYSDGQSETILGGLGLGLGGGDCRVKIATKANPWDGKSLKPDSVRSQLETSLKRLQCPQVDLFYL
  HAPDHGTPVEETLHACQRLHQEGKFVELGLSNYASWEVAEICTLCKSNGWILPTVYQGMYNATTRQVETE
  LFPCLRHFGLRFYAYNPLAGGLLTGKYKYEDKDGKQPVGRFFGNSWAETYRNRFWKEHHFEAIALVEKAL
  QAAYGASAPSVTSAALRWMYHHSQLQGAHGDAVILGMSSLEQLEQNLAATEEGPLEPAVVDAFNQAWHLV
  AHECPNYFR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003689
• ORF Size: 1077 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_003689.2, NP_003680.2
• RefSeq Size: 1377
• RefSeq ORF: 1080
• Locus ID: 8574
• Domains: aldo_ket_red
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene belongs to the aldo/keto reductase (AKR) superfamily and AKR7 family, which are involved in the detoxification of aldehydes and ketones. The AKR7 family consists of 3 genes that are present in a cluster on the p arm of chromosome 1. This protein, thought to be localized in the golgi, catalyzes the NADPH-dependent reduction of succinic semialdehyde to the endogenous neuromodulator, gamma-hydroxybutyrate. It may also function as a detoxication enzyme in the reduction of aflatoxin B1 and 2-carboxybenzaldehyde. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]