Shugoshin (SGO1) (NM_001012409) Human Tagged ORF Clone

CAT#: RG214977

SGOL1 (GFP-tagged) - Human shugoshin-like 1 (S. pombe) (SGOL1), transcript variant A1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SGO1
• Synonyms: CAID; NY-BR-85; SGO; SGOL1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214977 representing NM_001012409
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAGGAAAGATGCCTGAAAAAGTCCTTTCAAGATAGTCTTGAAGACATAAAGAAGCGAATGAAAG
  AGAAAAGGAATAAAAACTTGGCAGAGATTGGCAAACGCAGGTCTTTTATAGCTGCACCATGCCAAATAAT
  CACCAACACTTCTACACTGCTGAAAAATTACCAAGACAACAACAAAATGTTAGTTTTAGCTTTGGAAAAT
  GAAAAATCCAAAGTGAAAGAAGCCCAAGATATCATCCTACAGCTGAGAAAAGAATGTTACTATCTCACAT
  GTCAGCTATATGCATTGAAAGGAAAACTTACATCACAACAAACAGTAGAACCTGCTCAGAACCAGGAAAT
  ATGTTCCTCTGGAATGGACCCCAATAGTGATGACAGCTCCAGAAATTTATTTGTGAAGGATTTACCGCAA
  ATTCCTCTTGAAGAAACTGAACTTCCAGGACAAGGAGAATCATTTCAAATAGAAGATCAGATACCTACTA
  TTCCTCAAGACACACTGGGAGTTGATTTTGATTCAGGTGAAGCTAAGTCTACTGATAATGTCTTACCTAG
  AACTGTATCTGTTCGTAGCAGTTTAAAGAAACATTGTAACAGTATATGTCAGTTTGATAGCTTGGATGAT
  TTTGAAACCAGTCATTTGGCAGGGAAGTCTTTTGAATTCGAAAGAGTTGGATTTTTAGACCCACTAGTAA
  ACATGCACATACCTGAAAATGTACAACACAATGCTTGTCAATGGAGCAAGGACCAAGTTAACTTATCACC
  AAAGCTGATTCAGCCAGGAACGTTTACTAAAACAAAAGAAGACATTTTAGAATCTAAATCTGAACAAACT
  AAAAGTAAGCAAAGAGATACACAAGAAAGAAAAAGAGAAGAGAAAAGAAAAGCTAACAGGAGAAAATCAA
  AACGTATGTCAAAATATAAAGAGAATAAAAGCGAAAATAAAAAAACTGTTCCCCAAAAAAAAATGCACAA
  ATCTGTCAGTTCCAATGATGCTTACAATTTTAATTTGGAAGAGGGTGTTCATCTTACTCCTTTCCGACAA
  AAAGTGAGCAATGACTCTAATAGAGAAGAAAACAACGAGTCTGAAGTGAGCCTCTGTGAATCAAGTGGTT
  CAGGAGATGATTCCGATGACCTCTATTTGCCCACTTGCAAGTACATTCAGAATCCCACGAGCAATTCAGA
  TAGACCAGTCACCAGGCCTCTAGCTAAAAGAGCACTGAAATACACAGATGAAAAAGAGACGGAGGGTTCT
  AAGCCAACAAAAACTCCTACCACTACACCACCTGAAACTCAGCAGTCACCTCATCTTAGCCTGAAGGATA
  TCACCAATGTCTCCTTGTATCCTGTTGTGAAAATCAGAAGACTTTCTCTTTCTCCAAAAAAGAATAAAGC
  AAGCCCAGCAGTGGCTCTGCCTAAACGTAGGTGCACAGCCAGCGTGAACTATAAGGAGCCCACCCTCGCT
  TCGAAACTGAGAAGAGGGGACCCTTTTACAGATTTGTGTTTTTTGAATTCTCCTATTTTCAAGCAGAAAA
  AGGATTTGAGACGTTCTAAAAAAAGTATGAAACAAATACAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214977 representing NM_001012409
  Red=Cloning site Green=Tags(s)
  
  MAKERCLKKSFQDSLEDIKKRMKEKRNKNLAEIGKRRSFIAAPCQIITNTSTLLKNYQDNNKMLVLALEN
  EKSKVKEAQDIILQLRKECYYLTCQLYALKGKLTSQQTVEPAQNQEICSSGMDPNSDDSSRNLFVKDLPQ
  IPLEETELPGQGESFQIEDQIPTIPQDTLGVDFDSGEAKSTDNVLPRTVSVRSSLKKHCNSICQFDSLDD
  FETSHLAGKSFEFERVGFLDPLVNMHIPENVQHNACQWSKDQVNLSPKLIQPGTFTKTKEDILESKSEQT
  KSKQRDTQERKREEKRKANRRKSKRMSKYKENKSENKKTVPQKKMHKSVSSNDAYNFNLEEGVHLTPFRQ
  KVSNDSNREENNESEVSLCESSGSGDDSDDLYLPTCKYIQNPTSNSDRPVTRPLAKRALKYTDEKETEGS
  KPTKTPTTTPPETQQSPHLSLKDITNVSLYPVVKIRRLSLSPKKNKASPAVALPKRRCTASVNYKEPTLA
  SKLRRGDPFTDLCFLNSPIFKQKKDLRRSKKSMKQIQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012409
• ORF Size: 1581 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012409.1, NP_001012409.1
• RefSeq Size: 1956 bp
• RefSeq ORF: 1584 bp
• Locus ID: 151648
• Cytogenetics: 3p24.3
• Protein Pathways: Oocyte meiosis
• Gene Summary: The protein encoded by this gene is a member of the shugoshin family of proteins. This protein is thought to protect centromeric cohesin from cleavage during mitotic prophase by preventing phosphorylation of a cohesin subunit. Reduced expression of this gene leads to the premature loss of centromeric cohesion, mis-segregation of sister chromatids, and mitotic arrest. Evidence suggests that this protein also protects a small subset of cohesin found along the length of the chromosome arms during mitotic prophase. An isoform lacking exon 6 has been shown to play a role in the cohesion of centrioles (PMID: 16582621 and PMID:18331714). Mutations in this gene have been associated with Chronic Atrial and Intestinal Dysrhythmia (CAID) syndrome, characterized by the co-occurrence of Sick Sinus Syndrome (SSS) and Chronic Intestinal Pseudo-obstruction (CIPO) within the first four decades of life (PMID:25282101). Fibroblast cells from CAID patients exhibited both increased cell proliferation and higher rates of senescence. Pseudogenes of this gene have been found on chromosomes 1 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]