SAP1 (PTPRH) (NM_002842) Human Tagged ORF Clone

CAT#: RG215011

PTPRH (GFP-tagged) - Human protein tyrosine phosphatase, receptor type, H (PTPRH), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PTPRH
• Synonyms: R-PTP-H; SAP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215011 representing NM_002842
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGGGCTGGCGGGGGCCTCGGGGTCTGGGGGAACCTGGTGCTGCTGGGCCTGTGCAGCTGGACAG
  GGGCCAGGGCGCCTGCCCCCAACCCAGGGAGGAACCTGACAGTGGAGACTCAGACCACCAGCTCCATCTC
  CCTGAGCTGGGAGGTCCCCGATGGCCTAGACTCACAGAACTCCAACTACTGGGTTCAGTGTACTGGAGAC
  GGCGGCACAACAGAGACTCGAAACACAACAGCCACCAACGTCACCGTGGATGGCCTTGGACCCGGGTCAT
  TGTATACGTGTTCTGTGTGGGTGGAGAAAGACGGAGTAAATAGCTCTGTGGGGACTGTCACTACTGCCAC
  AGCTCCCAACCCAGTGAGGAACCTGAGAGTGGAGGCTCAGACCAACAGCTCCATCGCCCTGACCTGGGAG
  GTCCCCGACGGCCCAGACCCACAGAACTCCACCTACGGGGTTGAGTACACTGGAGATGGTGGCAGAGCAG
  GGACTCGAAGCACAGCACACACTAACATCACCGTGGATGGACTTGAACCCGGGTGTTTGTATGCGTTTTC
  CATGTGGGTGGGAAAGAATGGAATCAACAGCTCCCGGGAGACTCGAAATGCCACCACAGCTCACAACCCA
  GTGAGGAACCTGAGAGTGGAGGCTCAGACCACCAGCTCCATCTCCCTGAGCTGGGAGGTCCCCGATGGCA
  CAGACCCACAGAACTCGACCTACTGCGTTCAGTGCACTGGAGATGGTGGCAGAACAGAGACTCGAAACAC
  AACAGACACCAGAGTCACCGTGGATGGCCTTGGACCCGGGTCATTGTATACGTGTTCTGTGTGGGTGGAG
  AAAGACGGAGTAAATAGCTCTGTGGAGATTGTCACTAGTGCCACAGCTCCCAACCCAGTGAGAAACCTGA
  CAGTGGAGGCTCAGACCAACAGCTCCATCGCCCTGACCTGGGAGGTCCCCGATGGCCCAGACCCACAGAA
  CTCCACCTACGGGGTTGAGTACACTGGAGATGGTGGCAGAGCAGGGACTCGAAGCACAGCACACACCAAC
  ATCACCGTGGATAGACTTGAACCCGGGTGTTTGTATGTGTTTTCCGTGTGGGTGGGGAAGAATGGAATCA
  ACAGCTCCCGGGAGACTCGAAATGCCACCACAGCCCCCAACCCAGTGAGAAACCTCCATATGGAGACTCA
  GACCAACAGCTCCATCGCCCTATGCTGGGAAGTCCCCGATGGCCCATACCCTCAGGACTACACCTACTGG
  GTAGAGTACACTGGAGACGGTGGTGGCACAGAGACCCGAAACACAACAAATACCAGTGTGACAGCTGAGA
  GACTTGAGCCCGGAACCTTGTACACATTCTCTGTATGGGCAGAAAAAAATGGAGCACGTGGCTCCAGGCA
  GAATGTCAGCATCTCCACAGTCCCCAACGCAGTGACAAGCCTCAGCAAGCAGGACTGGACCAACAGCACC
  ATTGCTTTGCGCTGGACAGCTCCCCAGGGCCCAGGCCAGTCTTCCTACAGCTACTGGGTCTCATGGGTCA
  GGGAAGGCATGACTGACCCCAGGACCCAAAGCACCTCAGGTACTGACATCACCCTAAAGGAACTGGAAGC
  TGGCAGCCTGTACCACCTCACCGTCTGGGCCGAGAGGAATGAGGTCAGAGGCTATAACAGCACCCTCACT
  GCAGCCACTGCTCCCAATGAGGTCACAGATCTCCAGAATGAAACTCAGACTAAGAACTCAGTCATGCTGT
  GGTGGAAGGCCCCTGGAGACCCCCACTCTCAGTTGTACGTATACTGGGTCCAGTGGGCCAGCAAGGGACA
  TCCCCGGAGGGGGCAAGATCCCCAAGCGAATTGGGTCAACCAGACCAGCAGGACCAATGAGACGTGGTAC
  AAAGTGGAGGCCCTGGAACCCGGGACGTTGTACAATTTCACCGTGTGGGCAGAGAGGAATGACGTAGCCA
  GTTCCACGCAGAGCCTCTGTGCGTCCACATACCCAGACACAGTCACCATCACTTCCTGTGTCAGCACCTC
  AGCGGGCTATGGAGTCAACTTGATCTGGTCCTGCCCCCAGGGAGGCTACGAGGCCTTTGAGTTGGAGGTG
  GGAGGACAGCGGGGCTCCCAGGACAGATCTTCATGTGGGGAGGCTGTGTCTGTGTTGGGTCTCGGGCCGG
  CTCGGTCCTACCCAGCCACCATCACGACCATCTGGGACGGAATGAAGGTCGTGTCTCACTCTGTGGTCTG
  CCACACCGAGAGTGCAGGGGTCATTGCCGGAGCCTTTGTGGGCATCCTCCTGTTTCTCATCCTCGTGGGC
  CTGCTGATTTTCTTCCTGAAGAGGAGGAATAAGAAGAAGCAGCAGAAACCAGAACTCAGGGATCTGGTCT
  TTAGCTCCCCAGGGGACATCCCAGCTGAAGACTTCGCTGACCACGTCAGGAAGAATGAGAGGGACAGCAA
  CTGTGGTTTTGCAGACGAGTACCAGCAACTCTCCCTGGTGGGCCACAGCCAGTCTCAGATGGTGGCTTCG
  GCTTCAGAGAACAACGCCAAGAACCGCTACAGAAATGTGCTGCCCTATGACTGGTCCCGGGTGCCCCTGA
  AGCCCATCCATGAGGAGCCAGGCTCTGACTACATCAATGCCAGCTTCATGCCCGGTCTCTGGAGCCCCCA
  GGAGTTCATTGCAACCCAGGGTCCCCTGCCACAGACAGTGGGTGACTTCTGGCGCCTGGTGTGGGAACAG
  CAGAGCCACACCCTGGTCATGCTGACCAACTGCATGGAGGCCGGCCGGGTGAAGTGTGAGCATTACTGGC
  CTCTGGACTCGCAGCCCTGCACCCATGGGCACCTGCGGGTAACCCTGGTAGGTGAGGAAGTGATGGAGAA
  CTGGACGGTGCGGGAACTGCTGCTCCTCCAGGTGGAGGAGCAGAAGACACTGTCTGTGCGCCAATTCCAC
  TACCAGGCCTGGCCGGATCACGGCGTTCCCTCCTCCCCAGACACCTTGCTGGCTTTCTGGAGGATGCTTC
  GGCAGTGGCTGGATCAGACCATGGAGGGAGGCCCACCCATTGTGCACTGCAGTGCTGGCGTGGGTCGCAC
  AGGAACCCTCATTGCCCTGGACGTCCTGCTCCGGCAGCTGCAGTCCGAGGGTCTCCTTGGGCCCTTCAGC
  TTTGTAAGGAAGATGAGAGAGAGTCGGCCGTTGATGGTGCAGACTGAGGCTCAGTACGTATTCCTGCATC
  AGTGCATCCTGCGGTTCCTCCAACAGTCAGCCCAGGCCCCAGCCGAGAAGGAAGTCCCGTATGAGGATGT
  CGAAAACCTCATCTACGAGAACGTGGCCGCCATCCAGGCCCACAAGTTGGAGGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215011 representing NM_002842
  Red=Cloning site Green=Tags(s)
  
  MAGAGGGLGVWGNLVLLGLCSWTGARAPAPNPGRNLTVETQTTSSISLSWEVPDGLDSQNSNYWVQCTGD
  GGTTETRNTTATNVTVDGLGPGSLYTCSVWVEKDGVNSSVGTVTTATAPNPVRNLRVEAQTNSSIALTWE
  VPDGPDPQNSTYGVEYTGDGGRAGTRSTAHTNITVDGLEPGCLYAFSMWVGKNGINSSRETRNATTAHNP
  VRNLRVEAQTTSSISLSWEVPDGTDPQNSTYCVQCTGDGGRTETRNTTDTRVTVDGLGPGSLYTCSVWVE
  KDGVNSSVEIVTSATAPNPVRNLTVEAQTNSSIALTWEVPDGPDPQNSTYGVEYTGDGGRAGTRSTAHTN
  ITVDRLEPGCLYVFSVWVGKNGINSSRETRNATTAPNPVRNLHMETQTNSSIALCWEVPDGPYPQDYTYW
  VEYTGDGGGTETRNTTNTSVTAERLEPGTLYTFSVWAEKNGARGSRQNVSISTVPNAVTSLSKQDWTNST
  IALRWTAPQGPGQSSYSYWVSWVREGMTDPRTQSTSGTDITLKELEAGSLYHLTVWAERNEVRGYNSTLT
  AATAPNEVTDLQNETQTKNSVMLWWKAPGDPHSQLYVYWVQWASKGHPRRGQDPQANWVNQTSRTNETWY
  KVEALEPGTLYNFTVWAERNDVASSTQSLCASTYPDTVTITSCVSTSAGYGVNLIWSCPQGGYEAFELEV
  GGQRGSQDRSSCGEAVSVLGLGPARSYPATITTIWDGMKVVSHSVVCHTESAGVIAGAFVGILLFLILVG
  LLIFFLKRRNKKKQQKPELRDLVFSSPGDIPAEDFADHVRKNERDSNCGFADEYQQLSLVGHSQSQMVAS
  ASENNAKNRYRNVLPYDWSRVPLKPIHEEPGSDYINASFMPGLWSPQEFIATQGPLPQTVGDFWRLVWEQ
  QSHTLVMLTNCMEAGRVKCEHYWPLDSQPCTHGHLRVTLVGEEVMENWTVRELLLLQVEEQKTLSVRQFH
  YQAWPDHGVPSSPDTLLAFWRMLRQWLDQTMEGGPPIVHCSAGVGRTGTLIALDVLLRQLQSEGLLGPFS
  FVRKMRESRPLMVQTEAQYVFLHQCILRFLQQSAQAPAEKEVPYEDVENLIYENVAAIQAHKLEV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002842
• ORF Size: 3345 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002842.2, NP_002833.2
• RefSeq Size: 3895 bp
• RefSeq ORF: 3348 bp
• Locus ID: 5794
• Cytogenetics: 19q13.42
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: 'The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. The extracellular region contains eight fibronectin type III-like repeats and multiple N-glycosylation sites. The gene was shown to be expressed primarily in brain and liver, and at a lower level in heart and stomach. It was also found to be expressed in several cancer cell lines, but not in the corresponding normal tissues. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2009]'