GLT28D1 (ALG13) (NM_001099922) Human Tagged ORF Clone

CAT#: RG215034

ALG13 (GFP-tagged) - Human asparagine-linked glycosylation 13 homolog (S. cerevisiae) (ALG13), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ALG13
• Synonyms: CDG1S; CXorf45; EIEE36; GLT28D1; MDS031; TDRD13; YGL047W
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215034 representing NM_001099922
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTGCGTGTTTGTTACCGTAGGGACCACCAGCTTTGACGACCTCATTGCGTGTGTGTCGGCGCCCG
  ACAGTCTGCAAAAAATCGAGAGCCTTGGTTACAACCGACTTATCCTGCAAATTGGTAGAGGAACGGTGGT
  ACCTGAACCCTTCAGTACTGAGTCGTTTACTCTGGATGTTTACAGGTACAAGGATTCCTTGAAAGAAGAC
  ATTCAGAAAGCAGATCTTGTTATTAGTCACGCAGGTGCAGGAAGCTGTTTGGAGACTCTGGAAAAAGGAA
  AGCCACTCGTAGTGGTTATAAACGAAAAGTTGATGAACAATCATCAGCTGGAACTGGCAAAGCAGCTACA
  CAAAGAGGGTCATCTCTTCTATTGTACCTGCAGGGTCCTGACTTGTCCTGGGCAAGCCAAGTCCATTGCT
  TCTGCTCCTGGGAAGTGCCAAGATTCTGCAGCGCTGACTTCAACTGCCTTTTCAGGCCTAGACTTTGGGC
  TGCTTTCCGGATACCTGCATAAGCAAGCCCTTGTTACTGCTACCCATCCTACCTGCACCCTGCTTTTTCC
  CTCTTGCCACGCTTTTTTTCCTCTCCCTCTTACCCCCACCCTGTACAAAATGCATAAAGGATGGAAAAAC
  TACTGCAGCCAGAAGTCTTTGAATGAGGCATCAATGGATGAATATTTAGGCAGCTTAGGGCTGTTTCGAA
  AGCTGACTGCCAAGGATGCCTCTTGCCTCTTTCGGGCCATTTCGGAGCAGTTGTTTTGCAGCCAGGTCCA
  TCATTTGGAAATCAGGAAGGCTTGTGTCTCATATATGAGGGAAAATCAACAAACTTTTGAGTCTTATGTG
  GAGGGATCTTTTGAGAAATACCTGGAACGGTTGGGAGATCCCAAGGAAAGTGCTGGCCAGCTGGAAATAA
  GAGCTCTTTCTCTAATTTATAATCGGGATTTCATTCTTTATCGCTTTCCTGGAAAACCTCCAACTTATGT
  CACAGATAATGGCTATGAAGACAAGATTCTACTCTGCTACTCAAGTAGTGGTCACTATGATTCTGTGTAC
  TCAAAACAATTTCAGTCAAGTGCAGCTGTTTGTCAGGCTGTATTGTACGAAATTCTCTATAAAGATGTGT
  TTGTTGTGGATGAAGAAGAGTTGAAGACTGCGATTAAATTGTTTCGAAGTGGTTCTAAGAAGAACAGAAA
  TAATGCTGTAACTGGAAGCGAGGATGCCCATACTGATTACAAGAGTTCAAATCAGAATAGGATGGAAGAG
  TGGGGTGCCTGCTACAATGCTGAAAATATACCAGAGGGCTACAATAAAGGAACAGAAGAAACAAAGTCTC
  CAGAAAATCCATCAAAGATGCCCTTCCCCTATAAGGTGCTCAAAGCCCTGGATCCAGAAATCTATCGTAA
  TGTAGAATTTGATGTTTGGTTGGACAGCAGAAAAGAACTTCAAAAATCTGATTACATGGAGTATGCTGGG
  AGACAGTACTATTTGGGAGACAAGTGTCAGGTTTGCTTGGAATCAGAAGGAAGATATTATAATGCTCATA
  TCCAGGAAGTTGGAAATGAGAACAATTCAGTAACAGTCTTCATTGAAGAATTGGCGGAAAAGCATGTTGT
  TCCACTGGCTAACTTAAAACCAGTTACCCAAGTGATGTCTGTTCCTGCCTGGAATGCTATGCCCAGTCGG
  AAAGGAAGAGGTTACCAGAAAATGCCTGGGGGTTATGTCCCGGAAATAGTTATATCAGAGATGGACATAA
  AGCAACAGAAGAAGATGTTCAAGAAAATTCGAGGGAAAGAAGTTTACATGACTATGGCTTACGGCAAGGG
  AGACCCCCTCCTCCCACCCAGGCTGCAGCACAGTATGCATTATGGGCACGATCCTCCAATGCACTACTCA
  CAGACAGCTGGCAATGTTATGTCTAATGAACATTTTCATCCTCAGCATCCATCTCCGAGACAAGGTCGGG
  GATATGGGATGCCCAGGAATTCATCTCGGTTTATAAACAGGCACAACATGCCGGGCCCTAAAGTTGATTT
  TTACCCAGGCCCAGGTAAAAGGTGCTGCCAGAGCTATGATAACTTCTCTTATAGATCTCGTTCATTTAGA
  CGTAGTCACCGCCAGATGAGTTGTGTGAATAAGGAGTCCCAGTATGGATTTACCCCAGGGAATGGACAGA
  TGCCCAGGGGCTTGGAAGAAACTATTACTTTTTATGAAGTTGAAGAAGGGGATGAGACTGCTTATCCAAC
  TTTACCTAATCATGGAGGTCCCTCTACAATGGTTCCTGCTACTTCAGGATACTGTGTTGGAAGGCGGGGA
  CATAGCTCAGGCAAACAGACTTTGAATTTAGAGGAGGGCAATGGCCAGAGTGAAAATGGGCGATATCATG
  AAGAATATCTTTATCGTGCAGAGCCAGACTATGAAACTTCAGGTGTTTATAGCACAACTGCATCTACAGC
  AAACTTGTCTCTTCAGGACAGAAAGTCATGTTCTATGTCTCCTCAGGACACAGTTACCTCATACAACTAC
  CCCCAGAAGATGATGGGAAATATTGCAGCAGTTGCAGCTTCCTGTGCCAATAATGTTCCAGCTCCAGTCT
  TATCTAACGGTGCAGCGGCTAATCAAGCTATTAGTACCACTTCAGTTTCCTCACAGAATGCTATACAGCC
  TCTCTTTGTATCTCCACCTACACACGGCAGGCCAGTGATTGCCTCACCATCCTATCCATGCCATTCTGCT
  ATTCCTCATGCTGGTGCCTCTCTACCACCACCACCACCACCACCACCACCACCACCACCACCACCACCTC
  CTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTGCTCTTGATGTGGGAGAGACTTCAAACTTACAACC
  ACCACCACCACTACCACCTCCACCTTATTCCTGTGATCCAAGCGGCAGTGATTTGCCTCAAGATACAAAA
  GTTTTGCAGTACTATTTCAATCTAGGATTGCAGTGCTATTACCACAGCTACTGGCACTCCATGGTCTATG
  TGCCACAGATGCAGCAGCAGCTTCATGTAGAGAATTATCCAGTCTATACTGAGCCACCTCTGGTAGATCA
  AACCGTTCCTCAATGCTACAGTGAGGTGAGGAGAGAAGATGGCATACAGGCGGAAGCATCAGCAAATGAT
  ACTTTTCCGAATGCTGATTCTTCATCTGTCCCTCATGGAGCAGTCTATTATCCAGTAATGTCAGATCCCT
  ATGGGCAGCCACCTTTGCCAGGTTTTGACTCCTGCCTTCCGGTTGTGCCAGATTATTCCTGTGTTCCCCC
  CTGGCATCCAGTTGGTACAGCATATGGTGGTTCTTCTCAAATTCATGGTGCTATAAATCCTGGGCCAATT
  GGCTGTATTGCTCCATCTCCCCCAGCTTCTCATTATGTACCTCAGGGTATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215034 representing NM_001099922
  Red=Cloning site Green=Tags(s)
  
  MKCVFVTVGTTSFDDLIACVSAPDSLQKIESLGYNRLILQIGRGTVVPEPFSTESFTLDVYRYKDSLKED
  IQKADLVISHAGAGSCLETLEKGKPLVVVINEKLMNNHQLELAKQLHKEGHLFYCTCRVLTCPGQAKSIA
  SAPGKCQDSAALTSTAFSGLDFGLLSGYLHKQALVTATHPTCTLLFPSCHAFFPLPLTPTLYKMHKGWKN
  YCSQKSLNEASMDEYLGSLGLFRKLTAKDASCLFRAISEQLFCSQVHHLEIRKACVSYMRENQQTFESYV
  EGSFEKYLERLGDPKESAGQLEIRALSLIYNRDFILYRFPGKPPTYVTDNGYEDKILLCYSSSGHYDSVY
  SKQFQSSAAVCQAVLYEILYKDVFVVDEEELKTAIKLFRSGSKKNRNNAVTGSEDAHTDYKSSNQNRMEE
  WGACYNAENIPEGYNKGTEETKSPENPSKMPFPYKVLKALDPEIYRNVEFDVWLDSRKELQKSDYMEYAG
  RQYYLGDKCQVCLESEGRYYNAHIQEVGNENNSVTVFIEELAEKHVVPLANLKPVTQVMSVPAWNAMPSR
  KGRGYQKMPGGYVPEIVISEMDIKQQKKMFKKIRGKEVYMTMAYGKGDPLLPPRLQHSMHYGHDPPMHYS
  QTAGNVMSNEHFHPQHPSPRQGRGYGMPRNSSRFINRHNMPGPKVDFYPGPGKRCCQSYDNFSYRSRSFR
  RSHRQMSCVNKESQYGFTPGNGQMPRGLEETITFYEVEEGDETAYPTLPNHGGPSTMVPATSGYCVGRRG
  HSSGKQTLNLEEGNGQSENGRYHEEYLYRAEPDYETSGVYSTTASTANLSLQDRKSCSMSPQDTVTSYNY
  PQKMMGNIAAVAASCANNVPAPVLSNGAAANQAISTTSVSSQNAIQPLFVSPPTHGRPVIASPSYPCHSA
  IPHAGASLPPPPPPPPPPPPPPPPPPPPPPPPPPPALDVGETSNLQPPPPLPPPPYSCDPSGSDLPQDTK
  VLQYYFNLGLQCYYHSYWHSMVYVPQMQQQLHVENYPVYTEPPLVDQTVPQCYSEVRREDGIQAEASAND
  TFPNADSSSVPHGAVYYPVMSDPYGQPPLPGFDSCLPVVPDYSCVPPWHPVGTAYGGSSQIHGAINPGPI
  GCIAPSPPASHYVPQGM
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001099922
• ORF Size: 3411 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001099922.2, NP_001093392.1
• RefSeq Size: 4179
• RefSeq ORF: 3414
• Locus ID: 79868
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: The protein encoded by this gene is a subunit of a bipartite UDP-N-acetylglucosamine transferase. It heterodimerizes with asparagine-linked glycosylation 14 homolog to form a functional UDP-GlcNAc glycosyltransferase that catalyzes the second sugar addition of the highly conserved oligosaccharide precursor in endoplasmic reticulum N-linked glycosylation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009]