KCNH1 (NM_002238) Human Tagged ORF Clone

CAT#: RG215104

KCNH1 (GFP-tagged) - Human potassium voltage-gated channel, subfamily H (eag-related), member 1 (KCNH1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KCNH1
• Synonyms: EAG; EAG1; h-eag; hEAG1; Kv10.1; TMBTS; ZLS1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215104 representing NM_002238
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCATGGCTGGGGGCAGGAGGGGACTAGTGGCCCCTCAAAACACGTTTCTGGAGAATATTGTTCGGC
  GGTCCAATGATACTAATTTTGTGTTGGGGAATGCTCAGATAGTGGACTGGCCTATTGTGTACAGCAATGA
  TGGATTTTGCAAGCTGTCTGGCTATCACAGGGCAGAAGTGATGCAAAAAAGCAGCACCTGCAGTTTTATG
  TATGGGGAGCTGACTGATAAAGACACGATTGAAAAAGTGCGGCAAACATTTGAGAACTATGAGATGAATT
  CCTTTGAAATTCTGATGTACAAGAAGAACAGGACACCTGTGTGGTTCTTTGTGAAAATTGCTCCAATTCG
  AAACGAACAGGATAAAGTGGTTTTATTTCTTTGCACTTTCAGTGACATAACAGCTTTCAAACAGCCAATT
  GAGGATGATTCATGTAAAGGCTGGGGGAAGTTTGCTCGGCTGACAAGAGCACTGACAAGCAGCAGGGGTG
  TCCTGCAGCAGCTGGCTCCAAGCGTGCAAAAAGGCGAGAATGTCCACAAGCACTCCCGCCTGGCAGAGGT
  CCTACAGCTGGGCTCAGACATCCTTCCCCAGTACAAGCAAGAGGCACCAAAGACTCCCCCTCACATCATC
  TTACATTATTGTGTTTTTAAGACCACGTGGGATTGGATCATCTTGATCTTGACCTTCTATACAGCCATCT
  TGGTCCCTTATAATGTCTCCTTCAAAACCAGGCAGAATAATGTGGCCTGGCTGGTTGTTGATAGCATCGT
  GGATGTTATCTTTTTGGTGGACATTGTGCTCAATTTTCATACCACCTTTGTTGGACCAGCAGGGGAGGTG
  ATTTCTGACCCCAAACTTATCCGCATGAACTACCTGAAGACGTGGTTTGTGATTGACCTTCTGTCCTGTT
  TGCCATATGATGTCATCAACGCTTTTGAGAACGTGGATGAGGGCATCAGCAGCCTGTTCAGCTCTCTAAA
  AGTTGTCCGGCTGCTCCGTCTTGGGCGAGTGGCCCGTAAGCTGGACCACTACATTGAATATGGAGCTGCT
  GTGCTGGTCCTGCTGGTGTGTGTGTTTGGGCTGGCTGCACACTGGATGGCCTGCATCTGGTACAGCATTG
  GGGACTATGAGATCTTTGACGAGGACACCAAGACAATCCGCAACAACAGCTGGCTGTACCAACTAGCGAT
  GGACATTGGCACCCCTTACCAGTTTAATGGGTCTGGCTCAGGGAAGTGGGAAGGTGGTCCCAGCAAGAAT
  TCTGTCTACATCTCCTCGTTGTATTTCACAATGACCAGCCTCACCAGTGTGGGCTTTGGGAACATCGCCC
  CATCCACAGACATTGAGAAGATCTTTGCAGTGGCCATCATGATGATTGGCTCACTTCTCTATGCCACCAT
  CTTCGGGAATGTGACGACTATTTTCCAACAGATGTATGCCAACACCAACAGATACCATGAGATGCTCAAC
  AGTGTTCGGGACTTCCTGAAGCTCTACCAGGTGCCAAAAGGATTGAGTGAGCGAGTAATGGATTATATTG
  TGTCCACTTGGTCCATGTCCAGAGGCATTGACACAGAGAAGGTCCTGCAGATCTGCCCCAAGGACATGAG
  AGCCGACATCTGCGTGCACCTGAACCGCAAGGTGTTCAAGGAGCACCCGGCCTTCCGGCTGGCCAGTGAT
  GGCTGCCTCCGGGCACTGGCCATGGAGTTCCAGACGGTGCACTGTGCCCCAGGGGACCTCATCTACCATG
  CAGGAGAGAGCGTTGACAGCCTCTGCTTTGTGGTTTCTGGCTCCCTGGAGGTGATCCAAGATGATGAGGT
  GGTGGCCATTCTAGGAAAAGGAGACGTGTTTGGAGATGTGTTCTGGAAGGAAGCCACCCTTGCCCAGTCC
  TGTGCCAATGTTAGGGCCTTGACCTACTGTGATCTGCATGTGATCAAGCGGGATGCCCTGCAGAAAGTGC
  TGGAATTCTACACGGCCTTCTCCCATTCCTTCTCCCGGAACCTGATTCTGACGTACAACTTGAGGAAGAG
  GATTGTGTTCCGGAAGATCAGCGACGTGAAACGTGAAGAGGAAGAACGCATGAAACGAAAGAATGAGGCC
  CCCCTGATCTTGCCCCCGGACCACCCTGTCCGGCGCCTCTTCCAGAGATTCCGACAGCAGAAAGAGGCCA
  GGCTGGCAGCTGAGAGAGGGGGCCGGGACCTGGATGACCTAGATGTGGAGAAGGGCAATGTCCTTACAGA
  GCATGCCTCCGCCAACCACAGCCTCGTGAAGGCCAGCGTGGTCACCGTGCGTGAGAGTCCTGCCACGCCC
  GTATCCTTCCAGGCAGCCTCCACCTCCGGGGTGCCAGACCACGCAAAGCTACAGGCGCCAGGGTCCGAGT
  GCCTGGGCCCCAAGGGGGGCGGGGGCGATTGTGCCAAGCGCAAAAGCTGGGCCCGCTTCAAAGATGCTTG
  CGGGAAGAGTGAGGACTGGAACAAGGTGTCCAAGGCTGAGTCGATGGAGACACTTCCCGAGAGGACAAAA
  GCGTCAGGCGAGGCCACACTGAAGAAGACAGACTCGTGTGACAGTGGCATCACCAAGAGCGACTTGCGCC
  TGGACAACGTGGGTGAGGCCAGGAGTCCCCAGGATCGGAGTCCCATCCTGGCAGAGGTCAAGCATTCGTT
  CTACCCCATCCCTGAGCAGACGCTGCAGGCCACAGTCCTGGAGGTGAGGCACGAGCTGAAGGAGGACATC
  AAGGCCTTAAACGCCAAAATGACCAATATTGAGAAACAGCTCTCTGAGATACTCAGGATATTAACTTCCA
  GAAGATCCTCTCAGTCTCCTCAGGAGTTGTTTGAAATATCGAGGCCACAGTCCCCAGAATCAGAGAGAGA
  CATTTTTGGAGCCAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215104 representing NM_002238
  Red=Cloning site Green=Tags(s)
  
  MTMAGGRRGLVAPQNTFLENIVRRSNDTNFVLGNAQIVDWPIVYSNDGFCKLSGYHRAEVMQKSSTCSFM
  YGELTDKDTIEKVRQTFENYEMNSFEILMYKKNRTPVWFFVKIAPIRNEQDKVVLFLCTFSDITAFKQPI
  EDDSCKGWGKFARLTRALTSSRGVLQQLAPSVQKGENVHKHSRLAEVLQLGSDILPQYKQEAPKTPPHII
  LHYCVFKTTWDWIILILTFYTAILVPYNVSFKTRQNNVAWLVVDSIVDVIFLVDIVLNFHTTFVGPAGEV
  ISDPKLIRMNYLKTWFVIDLLSCLPYDVINAFENVDEGISSLFSSLKVVRLLRLGRVARKLDHYIEYGAA
  VLVLLVCVFGLAAHWMACIWYSIGDYEIFDEDTKTIRNNSWLYQLAMDIGTPYQFNGSGSGKWEGGPSKN
  SVYISSLYFTMTSLTSVGFGNIAPSTDIEKIFAVAIMMIGSLLYATIFGNVTTIFQQMYANTNRYHEMLN
  SVRDFLKLYQVPKGLSERVMDYIVSTWSMSRGIDTEKVLQICPKDMRADICVHLNRKVFKEHPAFRLASD
  GCLRALAMEFQTVHCAPGDLIYHAGESVDSLCFVVSGSLEVIQDDEVVAILGKGDVFGDVFWKEATLAQS
  CANVRALTYCDLHVIKRDALQKVLEFYTAFSHSFSRNLILTYNLRKRIVFRKISDVKREEEERMKRKNEA
  PLILPPDHPVRRLFQRFRQQKEARLAAERGGRDLDDLDVEKGNVLTEHASANHSLVKASVVTVRESPATP
  VSFQAASTSGVPDHAKLQAPGSECLGPKGGGGDCAKRKSWARFKDACGKSEDWNKVSKAESMETLPERTK
  ASGEATLKKTDSCDSGITKSDLRLDNVGEARSPQDRSPILAEVKHSFYPIPEQTLQATVLEVRHELKEDI
  KALNAKMTNIEKQLSEILRILTSRRSSQSPQELFEISRPQSPESERDIFGAS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002238
• ORF Size: 2886 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002238.2, NP_002229.1
• RefSeq Size: 3127 bp
• RefSeq ORF: 2889 bp
• Locus ID: 3756
• Cytogenetics: 1q32.2
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: 'Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a member of the potassium channel, voltage-gated, subfamily H. This member is a pore-forming (alpha) subunit of a voltage-gated non-inactivating delayed rectifier potassium channel. It is activated at the onset of myoblast differentiation. The gene is highly expressed in brain and in myoblasts. Overexpression of the gene may confer a growth advantage to cancer cells and favor tumor cell proliferation. Alternative splicing of this gene results in two transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]'