OR5A1 (NM_001004728) Human Tagged ORF Clone

CAT#: RG215187

OR5A1 (GFP-tagged) - Human olfactory receptor, family 5, subfamily A, member 1 (OR5A1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: OR5A1
• Synonyms: OR5A1P; OR11-249; OST181
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215187 representing NM_001004728
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCATAACCAAAGCCTGGAACAGCTCATCAGTGACCATGTTCATCCTCCTGGGATTCACAGACCATC
  CAGAACTCCAGGCCCTCCTCTTTGTGACCTTCCTGGGCATCTATCTTACCACCCTGGCCTGGAACCTGGC
  CCTCATTTTTCTGATCAGAGGTGACACCCATCTGCACACACCCATGTACTTCTTCCTAAGCAACTTATCT
  TTCATTGACATCTGCTACTCTTCTGCTGTGGCTCCCAATATGCTCACTGACTTCTTCTGGGAGCAGAAGA
  CCATATCATTTGTGGGCTGTGCTGCTCAGTTTTTTTTCTTTGTCGGCATGGGTCTGTCTGAGTGCCTCCT
  CCTGACTGCTATGGCATACGACCGATATGCAGCCATCTCCAGCCCCCTTCTCTACCCCACTATCATGACC
  CAGGGCCTCTGTACACGCATGGTGGTTGGGGCATATGTTGGTGGCTTCCTGAGCTCCCTGATCCAGGCCA
  GCTCCATATTTAGGCTTCACTTTTGCGGACCCAACATCATCAACCACTTCTTCTGCGACCTCCCACCAGT
  CCTGGCTCTGTCTTGCTCTGACACCTTCCTCAGTCAAGTGGTGAATTTCCTCGTGGTGGTCACTGTCGGA
  GGAACATCGTTCCTCCAACTCCTTATCTCCTATGGTTACATAGTGTCTGCGGTCCTGAAGATCCCTTCAG
  CAGAGGGCCGATGGAAAGCCTGCAACACGTGTGCCTCGCATCTGATGGTGGTGACTCTGCTGTTTGGGAC
  AGCCCTTTTCGTGTACTTGCGACCCAGCTCCAGCTACTTGCTAGGCAGGGACAAGGTGGTGTCTGTTTTC
  TATTCATTGGTGATCCCCATGCTGAACCCTCTCATTTACAGTTTGAGGAACAAAGAGATCAAGGATGCCC
  TGTGGAAGGTGTTGGAAAGGAAGAAAGTGTTTTCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215187 representing NM_001004728
  Red=Cloning site Green=Tags(s)
  
  MSITKAWNSSSVTMFILLGFTDHPELQALLFVTFLGIYLTTLAWNLALIFLIRGDTHLHTPMYFFLSNLS
  FIDICYSSAVAPNMLTDFFWEQKTISFVGCAAQFFFFVGMGLSECLLLTAMAYDRYAAISSPLLYPTIMT
  QGLCTRMVVGAYVGGFLSSLIQASSIFRLHFCGPNIINHFFCDLPPVLALSCSDTFLSQVVNFLVVVTVG
  GTSFLQLLISYGYIVSAVLKIPSAEGRWKACNTCASHLMVVTLLFGTALFVYLRPSSSYLLGRDKVVSVF
  YSLVIPMLNPLIYSLRNKEIKDALWKVLERKKVFS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004728
• ORF Size: 945 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004728.1, NP_001004728.1
• RefSeq Size: 948 bp
• RefSeq ORF: 948 bp
• Locus ID: 219982
• Cytogenetics: 11q12.1
• Protein Families: Transmembrane
• Protein Pathways: Olfactory transduction
• Gene Summary: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]