SAP97 (DLG1) (NM_004087) Human Tagged ORF Clone

CAT#: RG215258

DLG1 (GFP-tagged) - Human discs, large homolog 1 (Drosophila) (DLG1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DLG1
• Synonyms: DLGH1; hdlg; SAP-97; SAP97
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215258 representing NM_004087
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGTCCGGAAGCAAGATACCCAGAGAGCATTGCACCTTTTGGAGGAATATCGTTCAAAACTAAGCC
  AAACTGAAGACAGACAGCTCAGAAGTTCCATAGAACGGGTTATTAACATATTTCAGAGCAACCTCTTTCA
  GGCTTTAATAGATATTCAAGAATTTTATGAAGTGACCTTACTGGATAATCCAAAATGTATAGATCGTTCA
  AAGCCGTCTGAACCAATTCAACCTGTGAATACTTGGGAGATTTCCAGCCTTCCAAGCTCTACTGTGACTT
  CAGAGACACTGCCAAGCAGCCTTAGCCCTAGTGTAGAGAAATACAGGTATCAGGATGAAGATACACCTCC
  TCAAGAGCATATTTCCCCACAAATCACAAATGAAGTGATAGGTCCAGAATTGGTTCATGTCTCAGAGAAG
  AACTTATCAGAGATTGAGAATGTCCATGGATTTGTTTCTCATTCTCATATTTCACCAATAAAGGCAAATC
  CTCCCCCAGTACTGGTCAACACAGATAGCTTGGAAACACCAACTTACGTTAATGGCACAGATGCAGATTA
  TGAATATGAAGAAATCACACTTGAAAGGGGAAATTCAGGGCTTGGTTTCAGCATTGCAGGAGGTACGGAC
  AACCCACACATTGGAGATGACTCAAGTATTTTCATTACCAAAATTATCACAGGGGGAGCAGCCGCCCAAG
  ATGGAAGATTGCGGGTCAATGACTGTATATTACGAGTAAATGAAGTAGATGTTCGTGATGTAACACATAG
  CAAAGCAGTTGAAGCGTTGAAAGAAGCAGGGTCTATTGTACGCTTGTATGTAAAAAGAAGGAAACCAGTG
  TCAGAAAAAATAATGGAAATAAAGCTCATTAAAGGTCCTAAAGGTCTTGGGTTTAGCATTGCTGGAGGTG
  TTGGAAATCAGCATATTCCTGGGGATAATAGCATCTATGTAACCAAAATAATTGAAGGAGGTGCAGCACA
  TAAGGATGGCAAACTTCAGATTGGAGATAAACTTTTAGCAGTGAATAACGTATGTTTAGAAGAAGTTACT
  CATGAAGAAGCAGTAACTGCCTTAAAGAACACATCTGATTTTGTTTATTTGAAAGTGGCAAAACCCACAA
  GTATGTATATGAATGATGGCTATGCACCACCTGATATCACCAACTCTTCTTCTCAGCCTGTTGATAACCA
  TGTTAGCCCATCTTCCTTCTTGGGCCAGACACCAGCATCTCCAGCCAGATACTCCCCAGTTTCTAAAGCA
  GTACTTGGAGATGATGAAATTACAAGGGAACCTAGAAAAGTTGTTCTTCATCGTGGCTCAACGGGCCTTG
  GTTTCAACATTGTAGGAGGAGAAGATGGAGAAGGAATATTTATTTCCTTTATCTTAGCCGGAGGACCTGC
  TGATCTAAGTGGAGAGCTCAGAAAAGGAGATCGTATTATATCGGTAAACAGTGTTGACCTCAGAGCTGCT
  AGTCATGAGCAGGCAGCAGCTGCATTGAAAAATGCTGGCCAGGCTGTCACAATTGTTGCACAATATCGAC
  CTGAAGAATACAGTCGTTTTGAAGCTAAAATACATGATTTACGGGAGCAGATGATGAATAGTAGTATTAG
  TTCAGGGTCAGGTTCTCTTCGAACTAGCCAGAAGCGATCCCTCTATGTCAGAGCCCTTTTTGATTATGAC
  AAGACTAAAGACAGTGGGCTTCCCAGTCAGGGACTGAACTTCAAATTTGGAGATATCCTCCATGTTATTA
  ATGCTTCTGATGATGAATGGTGGCAAGCCAGGCAGGTTACACCAGATGGTGAGAGCGATGAGGTCGGAGT
  GATTCCCAGTAAACGCAGAGTTGAGAAGAAAGAACGAGCCCGATTAAAAACAGTGAAATTCAATTCTAAA
  ACGAGAGATAAAGGGTCATTCAATGACAAGCGTAAAAAGAACCTCTTTTCCCGAAAATTCCCCTTCTACA
  AGAACAAGGACCAGAGTGAGCAGGAAACAAGTGATGCTGACCAGCATGTAACTTCTAATGCCAGCGATAG
  TGAAAGTAGTTACCGTGGTCAAGAAGAATACGTCTTATCTTATGAACCAGTGAATCAACAAGAAGTTAAT
  TATACTCGACCAGTGATCATATTGGGACCTATGAAAGACAGGATAAATGATGACTTGATCTCAGAATTTC
  CTGACAAATTTGGATCCTGTGTTCCTCATACAACTAGACCAAAACGAGATTATGAGGTAGATGGAAGAGA
  TTATCATTTTGTGACTTCAAGAGAGCAGATGGAAAAAGATATCCAGGAACATAAATTCATTGAAGCTGGC
  CAGTATAACAATCATCTATATGGAACAAGTGTTCAGTCTGTACGAGAAGTAGCAGAAAAGGGCAAACACT
  GTATCCTTGATGTGTCTGGAAATGCCATAAAGAGATTACAGATTGCACAGCTTTACCCTATCTCCATTTT
  TATTAAACCCAAATCCATGGAAAATATCATGGAAATGAATAAGCGTCTAACAGAAGAACAAGCCAGAAAA
  ACATTTGAGAGAGCCATGAAACTGGAACAGGAGTTTACTGAACATTTCACAGCTATTGTACAGGGGGATA
  CGCTGGAAGACATTTACAACCAAGTGAAACAGATCATAGAAGAACAATCTGGTTCTTACATCTGGGTTCC
  GGCAAAAGAAAAGCTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215258 representing NM_004087
  Red=Cloning site Green=Tags(s)
  
  MPVRKQDTQRALHLLEEYRSKLSQTEDRQLRSSIERVINIFQSNLFQALIDIQEFYEVTLLDNPKCIDRS
  KPSEPIQPVNTWEISSLPSSTVTSETLPSSLSPSVEKYRYQDEDTPPQEHISPQITNEVIGPELVHVSEK
  NLSEIENVHGFVSHSHISPIKANPPPVLVNTDSLETPTYVNGTDADYEYEEITLERGNSGLGFSIAGGTD
  NPHIGDDSSIFITKIITGGAAAQDGRLRVNDCILRVNEVDVRDVTHSKAVEALKEAGSIVRLYVKRRKPV
  SEKIMEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGKLQIGDKLLAVNNVCLEEVT
  HEEAVTALKNTSDFVYLKVAKPTSMYMNDGYAPPDITNSSSQPVDNHVSPSSFLGQTPASPARYSPVSKA
  VLGDDEITREPRKVVLHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDRIISVNSVDLRAA
  SHEQAAAALKNAGQAVTIVAQYRPEEYSRFEAKIHDLREQMMNSSISSGSGSLRTSQKRSLYVRALFDYD
  KTKDSGLPSQGLNFKFGDILHVINASDDEWWQARQVTPDGESDEVGVIPSKRRVEKKERARLKTVKFNSK
  TRDKGSFNDKRKKNLFSRKFPFYKNKDQSEQETSDADQHVTSNASDSESSYRGQEEYVLSYEPVNQQEVN
  YTRPVIILGPMKDRINDDLISEFPDKFGSCVPHTTRPKRDYEVDGRDYHFVTSREQMEKDIQEHKFIEAG
  QYNNHLYGTSVQSVREVAEKGKHCILDVSGNAIKRLQIAQLYPISIFIKPKSMENIMEMNKRLTEEQARK
  TFERAMKLEQEFTEHFTAIVQGDTLEDIYNQVKQIIEEQSGSYIWVPAKEKL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004087
• ORF Size: 2778 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004087.1, NP_004078.1
• RefSeq Size: 3046 bp
• RefSeq ORF: 2781 bp
• Locus ID: 1739
• Cytogenetics: 3q29
• Domains: SH3, PDZ, L27, Guanylate_kin, GuKc
• Protein Families: Druggable Genome
• Protein Pathways: T cell receptor signaling pathway
• Gene Summary: 'This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. A multitude of transcript variants deriving from alternative splicing and the use of multiple alternate promoter have been observed, including some splice variants that may be specific to brain and other tissues. An upstream uORF may regulate translation at some splice variants of this gene. [provided by RefSeq, Sep 2018]'