SAP97 (DLG1) (NM_004087) Human Untagged Clone

CAT#: SC125478

DLG1 (untagged)-Human discs, large homolog 1 (Drosophila) (DLG1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DLG1
• Synonyms: DLGH1; hdlg; SAP-97; SAP97
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004087, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCGGTCCGGAAGCAAGATACCCAGAGAGCATTGCACCTTTTGGAGGAATATCGTTCAAAACTAAGCC
  AAACTGAAGACAGACAGCTCAGAAGTTCCATAGAACGGGTTATTAACATATTTCAGAGCAACCTCTTTCA
  GGCTTTAATAGATATTCAAGAATTTTATGAAGTGACCTTACTGGATAATCCAAAATGTATAGATCGTTCA
  AAGCCGTCTGAACCAATTCAACCTGTGAATACTTGGGAGATTTCCAGCCTTCCAAGCTCTACTGTGACTT
  CAGAGACACTGCCAAGCAGCCTTAGCCCTAGTGTAGAGAAATACAGGTATCAGGATGAAGATACACCTCC
  TCAAGAGCATATTTCCCCACAAATCACAAATGAAGTGATAGGTCCAGAATTGGTTCATGTCTCAGAGAAG
  AACTTATCAGAGATTGAGAATGTCCATGGATTTGTTTCTCATTCTCATATTTCACCAATAAAGCCAACAG
  AAGCTGTTCTTCCCTCTCCTCCCACTGTCCCTGTGATCCCTGTCCTGCCAGTCCCTGCTGAGAATACTGT
  CATCCTACCCACCATACCACAGGCAAATCCTCCCCCAGTACTGGTCAACACAGATAGCTTGGAAACACCA
  ACTTACGTTAATGGCACAGATGCAGATTATGAATATGAAGAAATCACACTTGAAAGGGGAAATTCAGGGC
  TTGGTTTCAGCATTGCAGGAGGTACGGACAACCCACACATTGGAGATGACTCAAGTATTTTCATTACCAA
  AATTATCACAGGGGGAGCAGCCGCCCAAGATGGAAGATTGCGGGTCAATGACTGTATATTACGAGTAAAT
  GAAGTAGATGTTCGTGATGTAACACATAGCAAAGCAGTTGAAGCGTTGAAAGAAGCAGGGTCTATTGTAC
  GCTTGTATGTAAAAAGAAGGAAACCAGTGTCAGAAAAAATAATGGAAATAAAGCTCATTAAAGGTCCTAA
  AGGTCTTGGGTTTAGCATTGCTGGAGGTGTTGGAAATCAGCATATTCCTGGGGATAATAGCATCTATGTA
  ACCAAAATAATTGAAGGAGGTGCAGCACATAAGGATGGCAAACTTCAGATTGGAGATAAACTTTTAGCAG
  TGAATAACGTATGTTTAGAAGAAGTTACTCATGAAGAAGCAGTAACTGCCTTAAAGAACACATCTGATTT
  TGTTTATTTGAAAGTGGCAAAACCCACAAGTATGTATATGAATGATGGCTATGCACCACCTGATATCACC
  AACTCTTCTTCTCAGCCTGTTGATAACCATGTTAGCCCATCTTCCTTCTTGGGCCAGACACCAGCATCTC
  CAGCCAGATACTCCCCAGTTTCTAAAGCAGTACTTGGAGATGATGAAATTACAAGGGAACCTAGAAAAGT
  TGTTCTTCATCGTGGCTCAACGGGCCTTGGTTTCAACATTGTAGGAGGAGAAGATGGAGAAGGAATATTT
  ATTTCCTTTATCTTAGCCGGAGGACCTGCTGATCTAAGTGGAGAGCTCAGAAAAGGAGATCGTATTATAT
  CGGTAAACAGTGTTGACCTCAGAGCTGCTAGTCATGAGCAGGCAGCAGCTGCATTGAAAAATGCTGGCCA
  GGCTGTCACAATTGTTGCACAATATCGACCTGAAGAATACAGTCGTTTTGAAGCTAAAATACATGATTTA
  CGGGAGCAGATGATGAATAGTAGTATTAGTTCAGGGTCAGGTTCTCTTCGAACTAGCCAGAAGCGATCCC
  TCTATGTCAGAGCCCTTTTTGATTATGACAAGACTAAAGACAGTGGGCTTCCCAGTCAGGGACTGAACTT
  CAAATTTGGAGATATCCTCCATGTTATTAATGCTTCTGATGATGAATGGTGGCAAGCCAGGCAGGTTACA
  CCAGATGGTGAGAGCGATGAGGTCGGAGTGATTCCCAGTAAACGCAGAGTTGAGAAGAAAGAACGAGCCC
  GATTAAAAACAGTGAAATTCAATTCTAAAACGAGAGATAAAGGGCAGTCATTCAATGACAAGCGTAAAAA
  GAACCTCTTTTCCCGAAAATTCCCCTTCTACAAGAACAAGGACCAGAGTGAGCAGGAAACAAGTGATGCT
  GACCAGCATGTAACTTCTAATGCCAGCGATAGTGAAAGTAGTTACCGTGGTCAAGAAGAATACGTCTTAT
  CTTATGAACCAGTGAATCAACAAGAAGTTAATTATACTCGACCAGTGATCATATTGGGACCTATGAAAGA
  CAGGATAAATGATGACTTGATCTCAGAATTTCCTGACAAATTTGGATCCTGTGTTCCTCATACAACTAGA
  CCAAAACGAGATTATGAGGTAGATGGAAGAGATTATCATTTTGTGACTTCAAGAGAGCAGATGGAAAAAG
  ATATCCAGGAACATAAATTCATTGAAGCTGGCCAGTATAACAATCATCTATATGGAACAAGTGTTCAGTC
  TGTACGAGAAGTAGCAGAAAAGGGCAAACACTGTATCCTTGATGTGTCTGGAAATGCCATAAAGAGATTA
  CAGATTGCACAGCTTTACCCTATCTCCATTTTTATTAAACCCAAATCCATGGAAAATATCATGGAAATGA
  ATAAGCGTCTAACAGAAGAACAAGCCAGAAAAACATTTGAGAGAGCCATGAAACTGGAACAGGAGTTTAC
  TGAACATTTCACAGCTATTGTACAGGGGGATACGCTGGAAGACATTTACAACCAAGTGAAACAGATCATA
  GAAGAACAATCTGGTTCTTACATCTGGGTTCCGGCAAAAGAAAAGCTATGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_004087
• Insert Size: 3000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004087.2, NP_004078.2
• RefSeq Size: 5034 bp
• RefSeq ORF: 2781 bp
• Locus ID: 1739
• Cytogenetics: 3q29
• Domains: SH3, PDZ, L27, Guanylate_kin, GuKc
• Protein Families: Druggable Genome
• Protein Pathways: T cell receptor signaling pathway
• Gene Summary: 'This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. A multitude of transcript variants deriving from alternative splicing and the use of multiple alternate promoter have been observed, including some splice variants that may be specific to brain and other tissues. An upstream uORF may regulate translation at some splice variants of this gene. [provided by RefSeq, Sep 2018]'
  Transcript Variant: This variant (2) contains an alternate in-frame coding exon compared to variant 1, resulting in a longer isoform (2) compared to isoform 1.