ADSSL1 (NM_199165) Human Tagged ORF Clone

CAT#: RG215329

ADSSL1 (GFP-tagged) - Human adenylosuccinate synthase like 1 (ADSSL1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ADSSL1
• Synonyms: Adss1; MPD5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215329 representing NM_199165
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGGGAGGAGCTGTGGGGTGGCAACCCAGAGGCAAGGAGGTGGGCAGAGGCCCACGAACCTGGCCC
  TGACCCTCAGCTCGTCCCCAGCTCACAGCACAGCCCTCCCCTGGCTGCCTCCAAGGAGTCTCCAGTTACT
  GAGTGGCCACTCCGTGCCAGCTCAGCCCACCCCTCACCTTCCCAGTGCCTGTGGAGGCCCAACTAGGGTG
  ACGCTGGGAGAGGAGAGGGCTTGGAGGAGCCACGGGTCAAATGCAGGAGGCCACACCTGCCTGCCCAGGA
  GGACTGCAGGAGCCGGATCCTTAACACCTGGAGGGGAGCGGGGGGGCAACAACGCCGGCCACACGGTGGT
  GGTGGATGGGAAAGAGTACGACTTCCACCTGCTGCCCAGCGGCATCATCAACACCAAGGCCGTGTCCTTC
  ATTGGCAACGGGGTGGTCATCCACTTGCCAGGCTTGTTTGAGGAAGCAGAGAAGAATGAAAAGAAAGGCC
  TGAAGGACTGGGAGAAGAGGCTCATCATCTCTGACAGAGCCCACCTTGTGTTTGATTTTCACCAGGCTGT
  CGACGGACTTCAGGAAGTGCAGCGCCAGGCACAAGAGGGGAAGAATATAGGCACCACCAAGAAGGGAATC
  GGACCAACCTACTCTTCCAAAGCTGCCCGGACAGGCCTCCGCATCTGCGACCTCCTGTCAGATTTTGATG
  AGTTTTCCTCCAGATTCAAGAACCTGGCCCACCAGCACCAGTCGATGTTCCCCACCCTGGAAATAGACAT
  TGAAGGCCAACTCAAAAGGCTCAAGGGCTTTGCTGAGCGGATCAGACCCATGGTCCGAGATGGTGTTTAC
  TTTATGTATGAGGCACTCCACGGCCCCCCCAAGAAGATCCTGGTGGAGGGTGCCAACGCCGCCCTCCTCG
  ACATTGACTTCGGGACCTACCCCTTTGTGACTTCATCCAACTGCACCGTGGGCGGTGTGTGCACGGGCCT
  GGGCATCCCCCCGCAGAACATAGGTGACGTGTATGGCGTGGTGAAAGCCTATACCACACGTGTGGGCATC
  GGGGCCTTCCCCACCGAGCAGATCAACGAGATTGGAGGCCTGCTGCAGACCCGCGGCCACGAGTGGGGAG
  TGACCACAGGCAGGAAGAGGCGCTGCGGCTGGCTCGACCTGATGATTCTAAGATATGCTCACATGGTCAA
  CGGATTCACTGCGCTGGCCCTGACGAAGCTGGACATCCTGGACGTACTGGGTGAGGTTAAAGTCGGTGTC
  TCATACAAGCTGAACGGGAAAAGGATTCCCTATTTCCCAGCTAACCAGGAGATGCTTCAGAAGGTCGAAG
  TTGAGTATGAAACGCTGCCTGGGTGGAAAGCAGACACCACAGGCGCCAGGAGGTGGGAGGACCTGCCCCC
  ACAGGCCCAGAACTACATCCGCTTTGTGGAGAATCACGTGGGAGTCGCAGTCAAATGGGTTGGTGTTGGC
  AAGTCAAGAGAGTCGATGATCCAGCTGTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215329 representing NM_199165
  Red=Cloning site Green=Tags(s)
  
  MVGRSCGVATQRQGGGQRPTNLALTLSSSPAHSTALPWLPPRSLQLLSGHSVPAQPTPHLPSACGGPTRV
  TLGEERAWRSHGSNAGGHTCLPRRTAGAGSLTPGGERGGNNAGHTVVVDGKEYDFHLLPSGIINTKAVSF
  IGNGVVIHLPGLFEEAEKNEKKGLKDWEKRLIISDRAHLVFDFHQAVDGLQEVQRQAQEGKNIGTTKKGI
  GPTYSSKAARTGLRICDLLSDFDEFSSRFKNLAHQHQSMFPTLEIDIEGQLKRLKGFAERIRPMVRDGVY
  FMYEALHGPPKKILVEGANAALLDIDFGTYPFVTSSNCTVGGVCTGLGIPPQNIGDVYGVVKAYTTRVGI
  GAFPTEQINEIGGLLQTRGHEWGVTTGRKRRCGWLDLMILRYAHMVNGFTALALTKLDILDVLGEVKVGV
  SYKLNGKRIPYFPANQEMLQKVEVEYETLPGWKADTTGARRWEDLPPQAQNYIRFVENHVGVAVKWVGVG
  KSRESMIQLF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_199165
• ORF Size: 1500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_199165.1, NP_954634.1
• RefSeq Size: 1826
• RefSeq ORF: 1503
• Locus ID: 122622
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Metabolic pathways, Purine metabolism
• Gene Summary: This gene encodes a member of the adenylosuccinate synthase family of proteins. The encoded muscle-specific enzyme plays a role in the purine nucleotide cycle by catalyzing the first step in the conversion of inosine monophosphate (IMP) to adenosine monophosphate (AMP). Mutations in this gene may cause adolescent onset distal myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]