PIK3CB (NM_006219) Human Tagged ORF Clone

CAT#: RG215433

PIK3CB (GFP-tagged) - Human phosphoinositide-3-kinase, catalytic, beta polypeptide (PIK3CB)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PIK3CB
• Synonyms: P110BETA; PI3K; PI3KBETA; PIK3C1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215433 representing NM_006219
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCTTCAGTTTCATAATGCCTCCTGCTATGGCAGACATCCTTGACATCTGGGCGGTGGATTCACAGA
  TAGCATCTGATGGCTCCATACCTGTGGATTTCCTTTTGCCCACTGGGATTTATATCCAGTTGGAGGTACC
  TCGGGAAGCTACCATTTCTTATATTAAGCAGATGTTATGGAAGCAAGTTCACAATTACCCAATGTTCAAC
  CTCCTTATGGATATTGACTCCTATATGTTTGCATGTGTGAATCAGACTGCTGTATATGAGGAGCTTGAAG
  ATGAAACACGAAGACTCTGTGATGTCAGACCTTTTCTTCCAGTTCTCAAATTAGTGACAAGAAGTTGTGA
  CCCAGGGGAAAAATTAGACTCAAAAATTGGAGTCCTTATAGGAAAAGGTCTGCATGAATTTGATTCCTTG
  AAGGATCCTGAAGTAAATGAATTTCGAAGAAAAATGCGCAAATTCAGCGAGGAAAAAATCCTGTCACTTG
  TGGGATTGTCTTGGATGGACTGGCTAAAACAAACATATCCACCAGAGCATGAACCATCCATCCCTGAAAA
  CTTAGAAGATAAACTTTATGGGGGAAAGCTCATCGTAGCTGTTCATTTTGAAAACTGCCAGGACGTGTTT
  AGCTTTCAAGTGTCTCCTAATATGAATCCTATCAAAGTAAATGAATTGGCAATCCAAAAACGTTTGACTA
  TTCATGGGAAGGAAGATGAAGTTAGCCCCTATGATTATGTGTTGCAAGTCAGCGGGAGAGTAGAATATGT
  TTTTGGTGATCATCCACTAATTCAGTTCCAGTATATCCGGAACTGTGTGATGAACAGAGCCCTGCCCCAT
  TTTATACTTGTGGAATGCTGCAAGATCAAGAAAATGTATGAACAAGAAATGATTGCCATAGAGGCTGCCA
  TAAATCGAAATTCATCTAATCTTCCTCTTCCATTACCACCAAAGAAAACACGAATTATTTCTCATGTTTG
  GGAAAATAACAACCCTTTCCAAATTGTCTTGGTTAAGGGAAATAAACTTAACACAGAGGAAACTGTAAAA
  GTTCATGTCAGGGCTGGTCTTTTTCATGGTACTGAGCTCCTGTGTAAAACCATCGTAAGCTCAGAGGTAT
  CAGGGAAAAATGATCATATTTGGAATGAACCACTGGAATTTGATATTAATATTTGTGACTTACCAAGAAT
  GGCTCGATTATGTTTTGCTGTTTATGCAGTTTTGGATAAAGTAAAAACGAAGAAATCAACGAAAACTATT
  AATCCCTCTAAATATCAGACCATCAGGAAAGCTGGAAAAGTGCATTATCCTGTAGCGTGGGTAAATACGA
  TGGTTTTTGACTTTAAAGGACAATTGAGAACTGGAGACATAATATTACACAGCTGGTCTTCATTTCCTGA
  TGAACTCGAAGAAATGTTGAATCCAATGGGAACTGTTCAAACAAATCCATATACTGAAAATGCAACAGCT
  TTGCATGTTAAATTTCCAGAGAATAAAAAACAACCTTATTATTACCCTCCCTTCGATAAGATTATTGAAA
  AGGCAGCTGAGATTGCAAGCAGTGATAGTGCTAATGTGTCAAGTCGAGGTGGAAAAAAGTTTCTTCCTGT
  ATTGAAAGAAATCTTGGACAGGGATCCCTTGTCTCAACTGTGTGAAAATGAAATGGATCTTATTTGGACT
  TTGCGACAAGACTGCCGAGAGATTTTCCCACAATCACTGCCAAAATTACTGCTGTCAATCAAGTGGAATA
  AACTTGAGGATGTTGCTCAGCTTCAGGCGCTGCTTCAGATTTGGCCTAAACTGCCCCCCCGGGAGGCCCT
  AGAGCTTCTGGATTTCAACTATCCAGACCAGTACGTTCGAGAATATGCTGTAGGCTGCCTGCGACAGATG
  AGTGATGAAGAACTTTCTCAATATCTTTTACAACTGGTGCAAGTGTTAAAATATGAGCCTTTTCTTGATT
  GTGCCCTCTCTAGATTCCTATTAGAAAGAGCACTTGGTAATCGGAGGATAGGGCAGTTTCTATTTTGGCA
  TCTTAGGTCAGAAGTGCACATTCCTGCTGTCTCAGTACAATTTGGTGTCATCCTTGAAGCATACTGCCGG
  GGAAGTGTGGGGCACATGAAAGTGCTTTCTAAGCAGGTTGAAGCACTCAATAAGTTAAAAACTTTAAATA
  GTTTAATCAAACTGAATGCCGTGAAGTTAAACAGAGCCAAAGGGAAGGAGGCCATGCATACCTGTTTAAA
  ACAGAGTGCTTACCGGGAAGCCCTCTCTGACCTGCAGTCACCCCTGAACCCATGTGTTATCCTCTCAGAA
  CTCTATGTTGAAAAGTGCAAATACATGGATTCCAAAATGAAGCCTTTGTGGCTGGTATACAATAACAAGG
  TATTTGGTGAGGATTCAGTTGGAGTGATTTTTAAAAATGGTGATGATTTACGACAGGATATGTTGACACT
  CCAAATGTTGCGCTTGATGGATTTACTCTGGAAAGAAGCTGGTTTGGATCTTCGGATGTTGCCTTATGGC
  TGTTTAGCAACAGGAGATCGCTCTGGCCTCATTGAAGTTGTGAGCACCTCTGAAACAATTGCTGACATTC
  AGCTGAACAGTAGCAATGTGGCTGCTGCAGCAGCCTTCAACAAAGATGCCCTTCTGAACTGGCTTAAAGA
  ATACAACTCTGGGGATGACCTGGACCGAGCCATTGAGGAATTTACACTGTCCTGTGCTGGCTACTGTGTA
  GCTTCTTATGTCCTTGGGATTGGTGACAGACATAGTGACAACATCATGGTCAAAAAAACTGGCCAGCTCT
  TCCACATTGACTTTGGACATATTCTTGGAAATTTCAAATCTAAGTTTGGCATTAAAAGGGAGCGAGTGCC
  TTTTATTCTTACCTATGATTTCATCCATGTCATTCAACAAGGAAAAACAGGAAATACAGAAAAGTTTGGC
  CGGTTCCGCCAGTGTTGTGAGGATGCATATCTGATTTTACGACGGCATGGGAATCTCTTCATCACTCTCT
  TTGCGCTGATGTTGACTGCAGGGCTTCCTGAACTCACATCAGTCAAAGATATACAGTATCTTAAGGACTC
  TCTTGCATTAGGGAAGAGTGAAGAAGAAGCACTCAAACAGTTTAAGCAAAAATTTGATGAGGCGCTCAGG
  GAAAGCTGGACTACTAAAGTGAACTGGATGGCCCACACAGTTCGGAAAGACTACAGATCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215433 representing NM_006219
  Red=Cloning site Green=Tags(s)
  
  MCFSFIMPPAMADILDIWAVDSQIASDGSIPVDFLLPTGIYIQLEVPREATISYIKQMLWKQVHNYPMFN
  LLMDIDSYMFACVNQTAVYEELEDETRRLCDVRPFLPVLKLVTRSCDPGEKLDSKIGVLIGKGLHEFDSL
  KDPEVNEFRRKMRKFSEEKILSLVGLSWMDWLKQTYPPEHEPSIPENLEDKLYGGKLIVAVHFENCQDVF
  SFQVSPNMNPIKVNELAIQKRLTIHGKEDEVSPYDYVLQVSGRVEYVFGDHPLIQFQYIRNCVMNRALPH
  FILVECCKIKKMYEQEMIAIEAAINRNSSNLPLPLPPKKTRIISHVWENNNPFQIVLVKGNKLNTEETVK
  VHVRAGLFHGTELLCKTIVSSEVSGKNDHIWNEPLEFDINICDLPRMARLCFAVYAVLDKVKTKKSTKTI
  NPSKYQTIRKAGKVHYPVAWVNTMVFDFKGQLRTGDIILHSWSSFPDELEEMLNPMGTVQTNPYTENATA
  LHVKFPENKKQPYYYPPFDKIIEKAAEIASSDSANVSSRGGKKFLPVLKEILDRDPLSQLCENEMDLIWT
  LRQDCREIFPQSLPKLLLSIKWNKLEDVAQLQALLQIWPKLPPREALELLDFNYPDQYVREYAVGCLRQM
  SDEELSQYLLQLVQVLKYEPFLDCALSRFLLERALGNRRIGQFLFWHLRSEVHIPAVSVQFGVILEAYCR
  GSVGHMKVLSKQVEALNKLKTLNSLIKLNAVKLNRAKGKEAMHTCLKQSAYREALSDLQSPLNPCVILSE
  LYVEKCKYMDSKMKPLWLVYNNKVFGEDSVGVIFKNGDDLRQDMLTLQMLRLMDLLWKEAGLDLRMLPYG
  CLATGDRSGLIEVVSTSETIADIQLNSSNVAAAAAFNKDALLNWLKEYNSGDDLDRAIEEFTLSCAGYCV
  ASYVLGIGDRHSDNIMVKKTGQLFHIDFGHILGNFKSKFGIKRERVPFILTYDFIHVIQQGKTGNTEKFG
  RFRQCCEDAYLILRRHGNLFITLFALMLTAGLPELTSVKDIQYLKDSLALGKSEEEALKQFKQKFDEALR
  ESWTTKVNWMAHTVRKDYRS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006219
• ORF Size: 3210 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006219.1, NP_006210.1
• RefSeq Size: 3213 bp
• RefSeq ORF: 3213 bp
• Locus ID: 5291
• Cytogenetics: 3q22.3
• Domains: PI3K_rbd, PI3_PI4_kinase, PI3Ka, PI3K_C2, PI3K_p85B
• Protein Families: Druggable Genome
• Protein Pathways: Acute myeloid leukemia, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Glioma, Inositol phosphate metabolism, Insulin signaling pathway, Jak-STAT signaling pathway, Leukocyte transendothelial migration, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Phosphatidylinositol signaling system, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus, VEGF signaling pathway
• Gene Summary: 'This gene encodes an isoform of the catalytic subunit of phosphoinositide 3-kinase (PI3K). These kinases are important in signaling pathways involving receptors on the outer membrane of eukaryotic cells and are named for their catalytic subunit. The encoded protein is the catalytic subunit for PI3Kbeta (PI3KB). PI3KB has been shown to be part of the activation pathway in neutrophils which have bound immune complexes at sites of injury or infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]'