PIK3CB (NM_006219) Human Untagged Clone

CAT#: SC116228

PIK3CB (untagged)-Human phosphoinositide-3-kinase, catalytic, beta polypeptide (PIK3CB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PIK3CB
• Synonyms: P110BETA; PI3K; PI3KBETA; PIK3C1
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006219 edited
  GAATTCGGCACGAGGGGGCTGCTGACTGCGCACTTGGAATAGTAGCAGGCGGCGGCGGCG
  GAACGCCAGGCAGGGCCCCGTGGTTATGAATGTGCTTCAGTTTCATAATGCCTCCTGCTA
  TGGCAGACATCCTTGACATCTGGGCGGTGGATTCACAGATAGCATCTGATGGCTCCATAC
  CTGTGGATTTCCTTTTGCCCACTGGGATTTATATCCAGTTGGAGGTACCTCGGGAAGCTA
  CCATTTCTTATATTAAGCAGATGTTATGGAAGCAAGTTCACAATTACCCAATGTTCAACC
  TCCTTATGGATATTGACTCCTATATGTTTGCATGTGTGAATCAGACTGCTGTATATGAGG
  AGCTTGAAGATGAAACACGAAGACTCTGTGATGTCAGACCTTTTCTTCCAGTTCTCAAAT
  TAGTGACAAGAAGTTGTGACCCAGGGGAAAAATTAGACTCAAAAATTGGAGTCCTTATAG
  GAAAAGGTCTGCATGAATTTGATTCCTTGAAGGATCCTGAAGTAAATGAATTTCGAAGAA
  AAATGCGCAAATTCAGCGAGGAAAAAATCCTGTCACTTGTGGGATTGTCTTGGATGGACT
  GGCTAAAACAAACATATCCACCAGAGCATGAACCATCCATCCCTGAAAACTTAGAAGATA
  AACTTTATGGGGGAAAGCTCATCGTAGCTGTTCATTTTGAAAACTGCCAGGACGTGTTTA
  GCTTTCAAGTGTCTCCTAATATGAATCCTATCAAAGTAAATGAATTGGCAATCCAAAAAC
  GTTTGACTATTCATGGGAAGGAAGATGAAGTTAGCCCCTATGATTATGTGTTGCAAGTCA
  GCGGGAGAGTAGAATATGTTTTTGGTGATCATCCACTAATTCAGTTCCAGTATATCCGGA
  ACTGTGTGATGAACAGAGCCCTGCCCCATTTTATACTTGTGGAATGCTGCAAGATCAAGA
  AAATGTATGAACAAGAAATGATTGCCATAGAGGCTGCCATAAATCGAAATTCATCTAATC
  TTCCTCTTCCATTACCACCAAAGAAAACACGAATTATTTCTCATGTTTGGGAAAATAACA
  ACCCTTTCCAAATTGTCTTGGTTAAGGGAAATAAACTTAACACAGAGGAAACTGTAAAAG
  TTCATGTCAGGGCTGGTCTTTTTCATGGTACTGAGCTCCTGTGTAAAACCATCGTAAGCT
  CAGAGGTATCAGGGAAAAATGATCATATTTGGAATGAACCACTGGAATTTGATATTAATA
  TTTGTGACTTACCAAGAATGGCTCGATTATGTTTTGCTGTTTATGCAGTTTTGGATAAAG
  TAAAAACGAAGAAATCAACGAAAACTATTAATCCCTCTAAATATCAGACCATCAGGAAAG
  CTGGAAAAGTGCATTATCCTGTAGCGTGGGTAAATACGATGGTTTTTGACTTTAAAGGAC
  AATTGAGAACTGGAGACATAATATTACACAGCTGGTCTTCATTTCCTGATGAACTCGAAG
  AAATGTTGAATCCAATGGGAACTGTTCAAACAAATCCATATACTGAAAATGCAACAGCTT
  TGCATGTTAAATTTCCAGAGAATAAAAAACAACCTTATTATTACCCTCCCTTCGATAAGA
  TTATTGAAAAGGCAGCTGAGATTGCAAGCAGTGATAGTGCTAATGTGTCAAGTCGAGGTG
  GAAAAAAGTTTCTTCCTGTATTGAAAGAAATCTTGGACAGGGATCCCTTGTCTCAACTGT
  GTGAAAATGAAATGGATCTTATTTGGACTTTGCGACAAGACTGCCGAGAGATTTTCCCAC
  AATCACTGCCAAAATTACTGCTGTCAATCAAGTGGAATAAACTTGAGGATGTTGCTCAGC
  TTCAGGCGCTGCTTCAGATTTGGCCTAAACTGCCCCCCCGGGAGGCCCTAGAGCTTCTGG
  ATTTCAACTATCCAGACCAGTACGTTCGAGAATATGCTGTAGGCTGCCTGCGACAGATGA
  GTGATGAAGAACTTTCTCAATATCTTTTACAACTGGTGCAAGTGTTAAAATATGAGCCTT
  TTCTTGATTGTGCCCTCTCTAGATTCCTATTAGAAAGAGCACTTGGTAATCGGAGGATAG
  GGCAGTTTCTATTTTGGCATCTTAGGTCAGAAGTGCACATTCCTGCTGTCTCAGTACAAT
  TTGGTGTCATCCTTGAAGCATACTGCCGGGGAAGTGTGGGGCACATGAAAGTGCTTTCTA
  AGCAGGTTGAAGCACTCAATAAGTTAAAAACTTTAAATAGTTTAATCAAACTGAATGCCG
  TGAAGTTAAACAGAGCCAAAGGGAAGGAGGCCATGCATACCTGTTTAAAACAGAGTGCTT
  ACCGGGAAGCCCTCTCTGACCTGCAGTCACCCCTGAACCCATGTGTTATCCTCTCAGAAC
  TCTATGTTGAAAAGTGCAAATACATGGATTCCAAAATGAAGCCTTTGTGGCTGGTATACA
  ATAACAAGGTATTTGGTGAGGATTCAGTTGGAGTGATTTTTAAAAATGGTGATGATTTAC
  GACAGGATATGTTGACACTCCAAATGTTGCGCTTGATGGATTTACTCTGGAAAGAAGCTG
  GTTTGGATCTTCGGATGTTGCCTTATGGCTGTTTAGCAACAGGAGATCGCTCTGGCCTCA
  TTGAAGTTGTGAGCACCTCTGAAACAATTGCTGACATTCAGCTGAACAGTAGCAATGTGG
  CTGCTGCAGCAGCCTTCAACAAAGATGCCCTTCTGAACTGGCTTAAAGAATACAACTCTG
  GGGATGACCTGGACCGAGCCATTGAGGAATTTACACTGTCCTGTGCTGGCTACTGTGTAG
  CTTCTTATGTCCTTGGGATTGGTGACAGACATAGTGACAACATCATGGTCAAAAAAACTG
  GCCAGCTCTTCCACATTGACTTTGGACATATTCTTGGAAATTTCAAATCTAAGTTTGGCA
  TTAAAAGGGAGCGAGTGCCTTTTATTCTTACCTATGATTTCATCCATGTCATTCAACAAG
  GAAAAACAGGAAATACAGAAAAGTTTGGCCGGTTCCGCCAGTGTTGTGAGGATGCATATC
  TGATTTTACGACGGCATGGGAATCTCTTCATCACTCTCTTTGCGCTGATGTTGACTGCAG
  GGCTTCCTGAACTCACATCAGTCAAAGATATACAGTATCTTAAGGACTCTCTTGCATTAG
  GGAAGAGTGAAGAAGAAGCACTCAAACAGTTTAAGCAAAAATTTGATGAGGCGCTCAGGG
  AAAGCTGGACTACTAAAGTGAACTGGATGGCCCACACAGTTCGGAAAGACTACAGATCTT
  AACGATCAGCCTTCGCTCCTAATGTATTTGTTGGTTTCATTTCATTTTCATTTTGCACTT
  GCACTAAATTGAACATGACCCTGTTAGAGATGTTATAAAGGGAATGAAATCCTGGAACTC
  AGAGTTAAATTAAGAACAAGGCATCCCACAGAACCTAATCTGAACAATCCCCGATGATTC
  CCTCTGCTTTTTGAATGCTTCCAAGACTTATCATGAAAACTGTCAATGGATAATCATTTC
  CTGCTGACTTTGCACGCCAAGGAATGCTACTAGGGATTGTTTCCGTTTTTGTTTGTTTTT
  TCTAATATTTGGTACTTCCCAGAATGGTGTAAATACTTCTTTTCAATGTTGTGACCAAGT
  ATTGTCACTCAGCCAACAACTTTTCCACACCTGGGGGGTTGGTGGCTGTTCTTACTGTCC
  AAATGAAGCTAAAAAGAAAGGCATCTTTCTTCCCTTTTAAAATTGTGTAAACTGCAAATT
  ATAATATAATTTGAATTTATGATTATTTTCCAGAAGAAATCTTGTAAACCTGTGGATACT
  CATTAATTCTTTTGTTAATATTTATTTCCATGATAGCATCATTCCAGCCAGACTTGCTGA
  AAATCTACTGGTGAGGCAAATATAATATATATAAATATGCTACATATATATTTATAAAAT
  TTCTAGTGGGAGTTCTATATAAATGTTTCTTTGGTATTCTTCAGCCTGTGATTTAAAGTT
  TTACAAAAAGCAGAGCTTTTTCCTAAGTTACTTTTCAGTTAGGTAACTGTGTGATCCAGT
  TCTTCCAGCTGCTTCTATAATGAGGCACATATTAATACAGTTTTTATATGGTATCTATGA
  AAGAGTTCACTTCATAGAGAATAATACTTGAGCAAATGTATCCAAGAAAGCAAGCAAATG
  AAAAGAAACCTATTTATGGAATAAACTCCAGATCTGAAATTCAGTATTTTAGAAAAATGC
  CAGCTCTTCTTACTGTATTTATTAAAACTTGTAATAATGTGATTTTTTTCAAGGATATTA
  GTTCAAATTGAAATGGTTTCACGCCACACGGAAATCTTTAAGTTATTTGTTGAGGTACCA
  TATATTTAGGGTGCTAGGGGCAAGTAATGTTAATATGTGCAATAGGAACTACTGGTTTGA
  ATGTGTAAATGGGTGATCTCTCTGAGTCCTGGCAACATCCAGCAAAACTACTGCTTATTC
  TCCAAAGAATATTGGGAGCTCTCAATCCTCGGTGATATGGGAAAGAGAACTGAGTATTTG
  CCCTATGACTGAGCTTTCTATAGGAATTTTATTAAAGAATGTTTAATTTTGTTGTCCTTC
  TTAATGTTCTCAGTCAAATAAATGAGTGAGCTGXXXXXXXXXXXXXXXXXXXXXXXXXXX
  XXXXXXXXXXXXXXXXXXXXXXAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006219 unedited
  GCACGAGGGGGCTGCTGACTGCGCACTTGGAATAGTAGCAGGCGGCGGCGGCGGAACGCC
  AGGCAGGGCCCCGTGGTTATGAATGTGCTTCAGTTTCATAATGCCTCCTGCTATGGCAGA
  CATCCTTGACATCTGGGCGGTGGATTCACAGATAGCATCTGATGGCTCCATACCTGTGGA
  TTTCCTTTTGCCCACTGGGATTTATATCCAGTTGGAGGTACCTCGGGAAGCTACCATTTC
  TTATATTAAGCAGATGTTATGGAAGCAAGTTCACAATTACCCAATGTTCAACCTCCTTAT
  GGATATTGACTCCTATATGTTTGCATGTGTGAATCAGACTGCTGTATATGAGGAGCTTGA
  AGATGAAACACGAAGACTCTGTGATGTCAGACCTTTTCTTCCAGTTCTCAAATTAGTGAC
  AAGAAGTTGTGACCCAGGGGAAAAATTAGACTCAAAAATTGGAGTCCTTATAGGAAAAGG
  TCTGCATGAATTTGATTCCTTGAAGGATCCTGAAGTAAATGAATTTCGAAGAANAATGCG
  CAAATTCAGCGAGGAAAAAATCCTGTCACTTGTGGGATTGTCTTGGATGGACTGGCTAAA
  ACAAACATATCCACC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006219
• Insert Size: 5000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006219.1, NP_006210.1
• RefSeq Size: 3213 bp
• RefSeq ORF: 3213 bp
• Locus ID: 5291
• Cytogenetics: 3q22.3
• Domains: PI3K_rbd, PI3_PI4_kinase, PI3Ka, PI3K_C2, PI3K_p85B
• Protein Families: Druggable Genome
• Protein Pathways: Acute myeloid leukemia, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Glioma, Inositol phosphate metabolism, Insulin signaling pathway, Jak-STAT signaling pathway, Leukocyte transendothelial migration, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Phosphatidylinositol signaling system, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus, VEGF signaling pathway
• Gene Summary: 'This gene encodes an isoform of the catalytic subunit of phosphoinositide 3-kinase (PI3K). These kinases are important in signaling pathways involving receptors on the outer membrane of eukaryotic cells and are named for their catalytic subunit. The encoded protein is the catalytic subunit for PI3Kbeta (PI3KB). PI3KB has been shown to be part of the activation pathway in neutrophils which have bound immune complexes at sites of injury or infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]'
  Transcript Variant: This variant (1) represent the longer transcript and encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.