ITGB1BP2 (NM_012278) Human Tagged ORF Clone

CAT#: RG215481

ITGB1BP2 (GFP-tagged) - Human integrin beta 1 binding protein (melusin) 2 (ITGB1BP2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ITGB1BP2
• Synonyms: CHORDC3; ITGB1BP; MELUSIN; MSTP015
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215481 representing NM_012278
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCTACTCTGTCGTAACAAAGGCTGTGGGCAGCACTTTGACCCTAATACCAACCTTCCTGATTCCT
  GTTGCCATCACCCTGGGGTCCCAATCTTCCATGATGCACTTAAGGGTTGGTCCTGCTGCCGAAAGCGAAC
  TGTAGATTTCTCTGAGTTCTTAAACATCAAGGGCTGTACTATGGGACCACACTGTGCTGAGAAGCTTCCT
  GAGGCCCCTCAACCTGAAGGCCCTGCTACAAGCAGTTCACTTCAGGAGCAAAAACCTCTGAATGTGATTC
  CAAAGTCAGCAGAGACCTTGCGCCGGGAGAGGCCCAAGTCAGAGTTGCCTCTGAAGCTGCTGCCGCTAAA
  TATATCCCAAGCCCTGGAAATGGCATTGGAACAGAAGGAATTAGACCAGGAACCTGGGGCAGGACTTGAC
  AGTCTGATCCGGACTGGTTCCAGCTGCCAGAACCCAGGATGTGATGCTGTTTACCAAGGCCCTGAGAGTG
  ATGCTACTCCATGTACCTACCACCCAGGAGCACCCCGATTCCATGAGGGGATGAAGTCTTGGAGCTGTTG
  TGGCATCCAGACCCTGGATTTTGGGGCATTTTTGGCACAACCAGGGTGCAGAGTCGGTAGACATGACTGG
  GGGAAGCAGCTCCCAGCATCTTGCCGCCATGATTGGCACCAGACAGATTCCTTAGTAGTGGTGACTGTAT
  ATGGCCAGATTCCACTTCCTGCGTTTAACTGGGTGAAGGCCAGTCAAACTGAGCTTCATGTCCACATTGT
  CTTTGATGGTAACCGTGTGTTCCAAGCACAGATGAAGCTCTGGGGGGTCATAAACGTGGAGCAGAGCTCT
  GTCTTCTTGATGCCATCTCGGGTTGAAATCTCCCTGGTCAAGGCTGACCCAGGATCCTGGGCCCAGCTGG
  AGCACCCTGATGCACTAGCTAAGAAGGCTAGGGCAGGGGTTGTGTTAGAGATGGATGAGGAAGAATCTGA
  CGATTCAGATGATGATCTGAGCTGGACAGAGGAGGAGGAAGAGGAGGAAGCAATGGGGGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215481 representing NM_012278
  Red=Cloning site Green=Tags(s)
  
  MSLLCRNKGCGQHFDPNTNLPDSCCHHPGVPIFHDALKGWSCCRKRTVDFSEFLNIKGCTMGPHCAEKLP
  EAPQPEGPATSSSLQEQKPLNVIPKSAETLRRERPKSELPLKLLPLNISQALEMALEQKELDQEPGAGLD
  SLIRTGSSCQNPGCDAVYQGPESDATPCTYHPGAPRFHEGMKSWSCCGIQTLDFGAFLAQPGCRVGRHDW
  GKQLPASCRHDWHQTDSLVVVTVYGQIPLPAFNWVKASQTELHVHIVFDGNRVFQAQMKLWGVINVEQSS
  VFLMPSRVEISLVKADPGSWAQLEHPDALAKKARAGVVLEMDEEESDDSDDDLSWTEEEEEEEAMGE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012278
• ORF Size: 1041 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012278.1, NP_036410.1
• RefSeq Size: 1235
• RefSeq ORF: 1044
• Locus ID: 26548
• Gene Summary: This gene encodes a protein with two cysteine and histidine-rich (CHORD) domains, PXXP motifs, YXXI/P motifs, putative SH2 and SH3 domain binding motifs, and an acidic region at the C-terminus that can bind calcium. Two hybrid analysis showed that this protein interacts with the cytoplasmic domain of the beta 1 integrin subunit and is thought to act as a chaperone protein. Studies in the mouse ortholog of this gene indicate that absence of this gene in mouse results in failed cardiac hypertrophy in response to mechanical stress. Alternative splicing results in multiple transcript variants encoding different isoforms, including an isoform that lacks several domains, including one of the CHORD domains. [provided by RefSeq, May 2017]