MCM10 (NM_182751) Human Tagged ORF Clone

CAT#: RG215642

MCM10 (GFP-tagged) - Human minichromosome maintenance complex component 10 (MCM10), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MCM10
• Synonyms: CNA43; DNA43; PRO2249
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215642 representing NM_182751
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGAGGAGGAAGACAATCTGTCTCTGCTGACCGCACTGCTGGAAGAAAATGAGTCAGCCTTGGATT
  GTAATTCAGAAGAAAATAACTTCTTGACGCGGGAAAATGGCGAGCCCGACGCATTTGATGAGCTCTTTGA
  TGCCGACGGCGACGGTGAATCTTATACAGAAGAGGCTGATGATGGAGAAACAGGAGAGACAAGAGACGAA
  AAGGAAAATCTGGCCACTCTCTTTGGAGATATGGAGGACTTAACAGATGAAGAAGAAGTTCCCGCATCAC
  AGTCAACTGAAAATAGGGTCCTCCCTGCTCCTGCCCCCAGGCGAGAGAAAACGAATGAAGAGTTGCAAGA
  GGAATTAAGGAATTTGCAAGAGCAAATGAAGGCCTTACAAGAGCAGCTAAAAGTAACAACAATTAAACAG
  ACAGCAAGCCCAGCCCGTCTGCAAAAATCCCCT:::GAGAAGTCTCCCCGGCCACCTCTTAAGGAGAGGA
  GAGTTCAGAGAATTCAGGAGTCAACATGCTTTTCTGCGGAGCTTGATGTCCCTGCGCTACCAAGAACCAA
  GAGGGTGGCTCGAACACCAAAGGCTTCACCTCCAGATCCCAAAAGCTCATCTTCAAGGATGACAAGTGCA
  CCCTCCCAACCCCTACAGACGATTTCTCGGAACAAACCTAGTGGGATAACTAGAGGTCAAATTGTGGGGA
  CCCCAGGAAGTTCTGGGGAAACGACTCAACCCATCTGTGTGGAAGCCTTCTCTGGTCTGCGGCTCAGGCG
  GCCTCGAGTATCCTCCACAGAAATGAACAAGAAAATGACCGGCCGAAAACTGATCAGACTGTCTCAGATC
  AAGGAAAAGATGGCCAGAGAGAAGCTGGAAGAAATAGATTGGGTGACATTTGGGGTTATATTGAAGAAGG
  TTACGCCACAGAGTGTGAATAGTGGAAAAACCTTCAGCATATGGAAACTGAATGATCTTCGTGACCTGAC
  ACAATGTGTGTCCTTGTTCTTATTTGGAGAAGTTCACAAAGCGCTCTGGAAGACGGAGCAGGGGACTGTC
  GTAGGGATCCTCAATGCCAACCCCATGAAGCCCAAGGATGGTTCAGAGGAGGTGTGTTTATCTATCGATC
  ATCCTCAGAAGGTCTTAATTATGGGTGAAGCTCTTGACCTGGGAACCTGTAAAGCCAAGAAGAAGAATGG
  AGAGCCGTGCACGCAGACTGTGAATTTGCGTGACTGTGAGTACTGTCAGTACCATGTCCAGGCTCAGTAC
  AAGAAGCTCAGCGCAAAGCGTGCGGATCTGCAGTCCACCTTCTCTGGAGGACGAATTCCAAAGAAGTTTG
  CCCGCAGAGGCACCAGCCTCAAAGAACGGCTGTGCCAAGATGGCTTTTACTACGGAGGGGTTTCTTCTGC
  CTCGTATGCAGCTTCAATTGCAGCAGCTGTGGCTCCTAAGAAGAAGATTCAAACCACTCTGAGTAATCTG
  GTTGTTAAGGGCACAAACTTGATCATCCAGGAAACACGGCAAAAACTCGGAATACCCCAGAAGAGCCTGT
  CTTGCTCTGAGGAGTTCAAGGAACTGATGGACCTGCCGACGTGTGGAGCCAGGAACTTAAAACAACATTT
  AGCCAAAGCCACAGCTTCAGGGATTATGGGGAGCCCAAAACCAGCCATCAAGTCCATCTCGGCCTCAGCA
  CTCTTGAAGCAACAGAAGCAGCGGATGTTGGAGATGAGGAGAAGGAAATCAGAAGAAATACAGAAGCGAT
  TTCTGCAGAGCTCAAGTGAAGTTGAGAGCCCAGCTGTGCCATCTTCATCAAGACAGCCCCCTGCTCAGCC
  TCCACGGACAGGATCCGAGTTCCCCAGGCTGGAGGGAGCCCCGGCCACAATGACGCCCAAGCTGGGGCGA
  GGTGTCTTGGAAGGAGATGATGTTCTCTTTTATGATGAGTCACCACCACCAAGACCAAAACTGAGTGCTT
  TAGCAGAAGCCAAAAAGTTAGCTGCTATCACCAAATTAAGGGCAAAAGGCCAGGTTCTTACAAAAACAAA
  CCCAAACAGCATTAAGAAGAAACAAAAGGACCCTCAGGACATCCTGGAGGTGAAGGAACGTGTAGAAAAA
  AACACCATGTTTTCTTCTCAAGCTGAGGATGAATTGGAGCCTGCCAGGAAAAAAAGGAGAGAACAACTTG
  CCTATCTGGAATCTGAGGAATTTCAGAAAATCCTAAAAGCAAAATCAAAACACACAGGCATCCTGAAAGA
  GGCCGAGGCTGAGATGCAGGAGCGCTACTTTGAGCCACTGGTGAAAAAAGAACAAATGGAAGAAAAGATG
  AGAAACATCAGAGAAGTGAAGTGCCGTGTCGTGACATGCAAGACGTGCGCCTATACCCACTTCAAGCTGC
  TGGAGACCTGCGTCAGTGAGCAGCATGAATACCACTGGCATGATGGTGTGAAGAGGTTTTTCAAATGTCC
  CTGTGGAAACAGAAGCATCTCCTTGGACAGACTCCCGAACAAGCACTGCAGTAACTGTGGCCTCTACAAA
  TGGGAACGGGACGGAATGCTAAAGGAAAAGACTGGTCCAAAGATAGGAGGAGAAACTCTGTTACCAAGAG
  GAGAAGAACATGCTAAATTTCTGAACAGCCTTAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215642 representing NM_182751
  Red=Cloning site Green=Tags(s)
  
  MDEEEDNLSLLTALLEENESALDCNSEENNFLTRENGEPDAFDELFDADGDGESYTEEADDGETGETRDE
  KENLATLFGDMEDLTDEEEVPASQSTENRVLPAPAPRREKTNEELQEELRNLQEQMKALQEQLKVTTIKQ
  TASPARLQKSPXEKSPRPPLKERRVQRIQESTCFSAELDVPALPRTKRVARTPKASPPDPKSSSSRMTSA
  PSQPLQTISRNKPSGITRGQIVGTPGSSGETTQPICVEAFSGLRLRRPRVSSTEMNKKMTGRKLIRLSQI
  KEKMAREKLEEIDWVTFGVILKKVTPQSVNSGKTFSIWKLNDLRDLTQCVSLFLFGEVHKALWKTEQGTV
  VGILNANPMKPKDGSEEVCLSIDHPQKVLIMGEALDLGTCKAKKKNGEPCTQTVNLRDCEYCQYHVQAQY
  KKLSAKRADLQSTFSGGRIPKKFARRGTSLKERLCQDGFYYGGVSSASYAASIAAAVAPKKKIQTTLSNL
  VVKGTNLIIQETRQKLGIPQKSLSCSEEFKELMDLPTCGARNLKQHLAKATASGIMGSPKPAIKSISASA
  LLKQQKQRMLEMRRRKSEEIQKRFLQSSSEVESPAVPSSSRQPPAQPPRTGSEFPRLEGAPATMTPKLGR
  GVLEGDDVLFYDESPPPRPKLSALAEAKKLAAITKLRAKGQVLTKTNPNSIKKKQKDPQDILEVKERVEK
  NTMFSSQAEDELEPARKKRREQLAYLESEEFQKILKAKSKHTGILKEAEAEMQERYFEPLVKKEQMEEKM
  RNIREVKCRVVTCKTCAYTHFKLLETCVSEQHEYHWHDGVKRFFKCPCGNRSISLDRLPNKHCSNCGLYK
  WERDGMLKEKTGPKIGGETLLPRGEEHAKFLNSLK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182751
• ORF Size: 2625 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_182751.1, NP_877428.1
• RefSeq Size: 4535
• RefSeq ORF: 2628
• Locus ID: 55388
• Protein Families: Stem cell - Pluripotency
• Gene Summary: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]