MCM10 (NM_182751) Human Untagged Clone

CAT#: SC309663

MCM10 (untagged)-Human minichromosome maintenance complex component 10 (MCM10), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MCM10
• Synonyms: CNA43; DNA43; PRO2249
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_182751 edited
  ATGGATGAGGAGGAAGACAATCTGTCTCTGCTGACCGCACTGCTGGAAGAAAATGAGTCA
  GCCTTGGATTGTAATTCAGAAGAAAATAACTTCTTGACGCGGGAAAATGGCGAGCCCGAC
  GCATTTGATGAGCTCTTTGATGCCGACGGCGACGGTGAATCTTATACAGAAGAGGCTGAT
  GATGGAGAAACAGGAGAGACAAGAGACGAAAAGGAAAATCTGGCCACTCTCTTTGGAGAT
  ATGGAGGACTTAACAGATGAAGAAGAAGTTCCCGCATCACAGTCAACTGAAAATAGGGTC
  CTCCCTGCTCCTGCCCCCAGGCGAGAGAAAACGAATGAAGAGTTGCAAGAGGAATTAAGG
  AATTTGCAAGAGCAAATGAAGGCCTTACAAGAGCAGCTAAAAGTAACAACAATTAAACAG
  ACAGCAAGCCCAGCCCGTCTGCAAAAATCCCCT:::GAGAAGTCTCCCCGGCCACCTCTT
  AAGGAGAGGAGAGTTCAGAGAATTCAGGAGTCAACATGCTTTTCTGCGGAGCTTGATGTC
  CCTGCGCTACCAAGAACCAAGAGGGTGGCTCGAACACCAAAGGCTTCACCTCCAGATCCC
  AAAAGCTCATCTTCAAGGATGACAAGTGCACCCTCCCAACCCCTACAGACGATTTCTCGG
  AACAAACCTAGTGGGATAACTAGAGGTCAAATTGTGGGGACCCCAGGAAGTTCTGGGGAA
  ACGACTCAACCCATCTGTGTGGAAGCCTTCTCTGGTCTGCGGCTCAGGCGGCCTCGAGTA
  TCCTCCACAGAAATGAACAAGAAAATGACCGGCCGAAAACTGATCAGACTGTCTCAGATC
  AAGGAAAAGATGGCCAGAGAGAAGCTGGAAGAAATAGATTGGGTGACATTTGGGGTTATA
  TTGAAGAAGGTTACGCCACAGAGTGTGAATAGTGGAAAAACCTTCAGCATATGGAAACTG
  AATGATCTTCGTGACCTGACACAATGTGTGTCCTTGTTCTTATTTGGAGAAGTTCACAAA
  GCGCTCTGGAAGACGGAGCAGGGGACTGTCGTAGGGATCCTCAATGCCAACCCCATGAAG
  CCCAAGGATGGTTCAGAGGAGGTGTGTTTATCTATCGATCATCCTCAGAAGGTCTTAATT
  ATGGGTGAAGCTCTTGACCTGGGAACCTGTAAAGCCAAGAAGAAGAATGGAGAGCCGTGC
  ACGCAGACTGTGAATTTGCGTGACTGTGAGTACTGTCAGTACCATGTCCAGGCTCAGTAC
  AAGAAGCTCAGCGCAAAGCGTGCGGATCTGCAGTCCACCTTCTCTGGAGGACGAATTCCA
  AAGAAGTTTGCCCGCAGAGGCACCAGCCTCAAAGAACGGCTGTGCCAAGATGGCTTTTAC
  TACGGAGGGGTTTCTTCTGCCTCGTATGCAGCTTCAATTGCAGCAGCTGTGGCTCCTAAG
  AAGAAGATTCAAACCACTCTGAGTAATCTGGTTGTTAAGGGCACAAACTTGATCATCCAG
  GAAACACGGCAAAAACTCGGAATACCCCAGAAGAGCCTGTCTTGCTCTGAGGAGTTCAAG
  GAACTGATGGACCTGCCGACGTGTGGAGCCAGGAACTTAAAACAACATTTAGCCAAAGCC
  ACAGCTTCAGGGATTATGGGGAGCCCAAAACCAGCCATCAAGTCCATCTCGGCCTCAGCA
  CTCTTGAAGCAACAGAAGCAGCGGATGTTGGAGATGAGGAGAAGGAAATCAGAAGAAATA
  CAGAAGCGATTTCTGCAGAGCTCAAGTGAAGTTGAGAGCCCAGCTGTGCCATCTTCATCA
  AGACAGCCCCCTGCTCAGCCTCCACGGACAGGATCCGAGTTCCCCAGGCTGGAGGGAGCC
  CCGGCCACAATGACGCCCAAGCTGGGGCGAGGTGTCTTGGAAGGAGATGATGTTCTCTTT
  TATGATGAGTCACCACCACCAAGACCAAAACTGAGTGCTTTAGCAGAAGCCAAAAAGTTA
  GCTGCTATCACCAAATTAAGGGCAAAAGGCCAGGTTCTTACAAAAACAAACCCAAACAGC
  ATTAAGAAGAAACAAAAGGACCCTCAGGACATCCTGGAGGTGAAGGAACGTGTAGAAAAA
  AACACCATGTTTTCTTCTCAAGCTGAGGATGAATTGGAGCCTGCCAGGAAAAAAAGGAGA
  GAACAACTTGCCTATCTGGAATCTGAGGAATTTCAGAAAATCCTAAAAGCAAAATCAAAA
  CACACAGGCATCCTGAAAGAGGCCGAGGCTGAGATGCAGGAGCGCTACTTTGAGCCACTG
  GTGAAAAAAGAACAAATGGAAGAAAAGATGAGAAACATCAGAGAAGTGAAGTGCCGTGTC
  GTGACATGCAAGACGTGCGCCTATACCCACTTCAAGCTGCTGGAGACCTGCGTCAGTGAG
  CAGCATGAATACCACTGGCATGATGGTGTGAAGAGGTTTTTCAAATGTCCCTGTGGAAAC
  AGAAGCATCTCCTTGGACAGACTCCCGAACAAGCACTGCAGTAACTGTGGCCTCTACAAA
  TGGGAACGGGACGGAATGCTAAAGGAAAAGACTGGTCCAAAGATAGGAGGAGAAACTCTG
  TTACCAAGAGGAGAAGAACATGCTAAATTTCTGAACAGCCTTAAATAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_182751 unedited
  GGTATAGATGCTACACGACATACATCTAGGGCGGCCGCGACATCGGCACGAGGGGCGATC
  CCGGCATCGGCCAAGATTCTACATCGCTCATCTGGGCATCTGAGCCTCCTTCGAAGCTTC
  CTGTCACAACTGTCCTCTTGACAGCATGGATGAGGAGGAAGACAATCTGTCTCTGCTGAC
  CGCACTGCTGGAAGAAAATGAGTCAGCCTTGGATTGTAATTCAGAAGAAAATAACTTCTT
  GACGCGGGAAAATGGCGAGCCCGACGCATTTGATGAGCTCTTTGATGCCGACGGCGACGG
  TGAATCTTATACAGAAGAGGCTGATGATGGAGAAACAGGAGAGACAAGAGACGAAAAGGA
  AAATCTGGCCACTCTCTTTGGAGATATGGAGGACTTAACAGATGAAGAAGAAGTTCCCGC
  ATCACAGTCAACTGAAAATAGGGTCCTCCCTGCTCCTGCCCCCAGGCGAGAGAAAACGAA
  TGAAGAGTTGCAAGAGGAATTAAGGAATTTGCAAGAGCAAATGAAGGCCTTACAAGAGCA
  GCTAAAAGTAACAACAATTAAACAGACAGCAAGCCCAGCCCGTCTGCAAAAATCCCCTGA
  GAAGTCTCCCCGGCCACCTCTTAAGGAGAGGAGAGTTCAGAGAATTCAGGAGTCAACATG
  CTTTTCTGCGGAGCTTGATGTCCCTGCGCTACCAAGAACCAAGAGGGTGGCTCGAACACC
  AAAGGCTTCACCTCCAGATCCCAAAAGCTCATCTTCAAAGATGACAAGTGCACCCTCCCA
  ACCCCTACAGACGATTTCTCGGACAAACCTAGTGGGATAACTAGAGGTCAAATTGTGGGG
  ACCCCAGGAAGTTCTGGGGAAACGACTCAACCCATCTGTGTGGAGCCTTCTCTGGTCTGC
  GCTCAGCGCCTCGAGTATCCTCCACAGAATGAACAGAATGACCGGCCGAAAACTGATCAG
  ACTGTTCCTCAG
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_182751 unedited
  CGACGACCAGACAAATGACGCCCAAGCTGGGGCGAGGTGTCTTGGAAGGAGATGATGTTC
  TCTTTTATGATGAGTCACCACCACCAAGACCAAAACTGAGTGCTTTAGCAGAAGCCAAAA
  AGTTAGCTGCTATCACCAAATTAAGGGCAAAAGGCCAGGTTCTTACAAAAACAAACCCAA
  ACAGCATTAAGAAGAAACAAAAGGACCCTCAGGACATCCTGGAGGTGAAGGAACGTGTAG
  AAAAAAACACCATGTTTTCTTCTCAAGCTGAGGATGAATTGGAGCCTGCCAGGAAAAAAA
  GGAGAGAACAACTTGCCTATCTGGAATCTGAGGAATTTCAGAAAATCCTAAAAGCAAAAT
  CAAAACACACAGGCATCCTGAAAGAGGCCGAGGCTGAGATGCAGGAGCGCTACTTTGAGC
  CACTGGTGAAAAAAGAACAAATGGAAGAAAAGATGAGAAACATCAGAGAAGTGAAGTGCC
  GTGTCGTGACATGCAAGACGTGCGCCTATACCCACTTCAAGCTGCTGGAGACCTGCGTCA
  GTGAGCAGCATGAATACCACTGGCATGATGGTGTGAAGAGGTTTTTCAAATGTCCCTGTG
  GAAACAGAAGCATCTCCTTGGACAGACTCCCGAACAAGCACTGCAGTAACTGTGGCCTCT
  ACAAATGGGAACGGGACGGAATGCTAAAGGAAAAGACTGGTCCAAAGATAGGAGGAGAAA
  CTCTGTTACCAAGAGGAGAGACATGCTAAATTTCTGACAGCCTTAAATAACCCGAACTTC
  AGACATTTTCCCACAGACTTCCTGGCCTCCTGTGACTCTGGAAAGCAAAGATTGCTGTGT
  ATTGTCCATTGATCCTGATTGACGCGTCAAAACAAATGCTTGTTAAGCCCATAAGCTTTG
  CCTGCTACTTTCTGCCATTGGGTTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_182751
• ORF Size: 2628 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to differ from the protein associated to this reference by a single amino acid.

Reference Data

• RefSeq: NM_182751.1, NP_877428.1
• RefSeq Size: 4535
• RefSeq ORF: 2628
• Locus ID: 55388
• Protein Families: Stem cell - Pluripotency
• Gene Summary: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) uses a different splice site, compared to variant 2, that results in a longer isoform (1). Though this variant is supported by EST data, the existence of its encoded isoform has not been experimentally verified.