CDC2L1 (CDK11B) (NM_033492) Human Tagged ORF Clone

CAT#: RG215881

CDK11B (GFP-tagged) - Human cyclin-dependent kinase 11B (CDK11B), transcript variant 8. Note: ORF is codon optimized

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CDK11B
• Synonyms: CDC2L1; CDK11; CDK11-p46; CDK11-p58; CDK11-p110; CLK-1; p58; p58CDC2L1; p58CLK-1; PK58
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215881 ORF sequence, codon optimized.
  Due to the complexity of NM_033492, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGATGAAAAAGATTCATGGAAGGTGAAGACCCTCGATGAGATCCTTCAGGAAAAGAAACGCCGCA
  AAGAGCAAGAAGAGAAAGCGGAGATCAAGCGACTGAAAAACTCTGATGATCGGGATTCCAAGAGGGACTC
  CCTGGAGGAGGGAGAGTTGCGGGACCACTGTATGGAAATAACAATCAGGAACAGCCCATATCGACGGGAA
  GACAGCATGGAAGACAGGGGCGAAGAAGACGATTCCCTCGCCATCAAGCCTCCCCAGCAAATGTCCAGAA
  AGGAGAAAGTGCACCATAGGAAGGATGAGAAGCGGAAGGAAAAAAAACACGCCCGAGTCAAAGAAAAAGA
  GAGAGAGCACGAGAGAAGAAAACGGCACCGCGAGGAGCAGGATAAAGCGCGGCGAGAATGGGAAAGACAG
  AAAAGAAGAGAGATGGCGCGGGAACATTCAAGAAGGGAAAGGGGTAATGACGGGGTTTGCTTGTTCCGCG
  ATCGGCTCGAACAGCTCGAAAGAAAGAGGGAAAGAGAAAGAAAGATGAGAGAACAGCAGAAGGAACAGAG
  AGAACAAAAAGAGAGAGAGAGGCGCGCCGAGGAGAGGAGGAAGGAGAGGGAAGCCCGCAGAGAAGTCAGC
  GCCCACCATAGAACTATGAGAGAGGACTACAGCGACAAAGTCAAGGCATCACATTGGAGCCGCAGCCCTC
  CACGACCACCTAGAGAAAGGTTCGAGTTGGGCGATGGAAGAAAACCAGTTAAAGAGGAAAAAATGGAGGA
  GAGAGATCTGCTTAGCGATCTCCAGGACATCAGTGATAGCGAACGAAAAACTAGCTCCGCTGAGTCATCA
  TCTGCAGAGAGCGGTTCAGGATCCGAGGAGGAGGAGGAAGAAGAAGAGGAGGAAGAGGAGGAGGGGAGCA
  CGAGCGAGGAGTCCGAGGAGGAAGAGGAAGAGGAGGAGGAGGAAGAAGAAGAGACAGGGAGTAACTCCGA
  GGAGGCCTCTGAGCAGTCAGCCGAGGAAGTCTCAGAAGAGGAGATGTCCGAAGACGAGGAGCGGGAGAAT
  GAGAACCATTTGCTGGTGGTCCCAGAATCACGCTTTGATAGGGATTCTGGAGAGTCCGAGGAAGCTGAAG
  AAGAAGTGGGGGAAGGTACACCACAGTCATCCGCACTGACCGAGGGCGACTATGTGCCGGATTCCCCAGC
  CCTGAGCCCAATTGAGCTGAAGCAGGAACTGCCCAAATATCTGCCCGCCCTGCAGGGGTGCCGGTCAGTT
  GAGGAATTCCAGTGCCTCAACAGAATTGAGGAAGGGACGTACGGTGTGGTCTACCGAGCTAAAGACAAAA
  AGACTGACGAAATCGTCGCTCTGAAGCGGCTGAAGATGGAAAAAGAGAAAGAGGGGTTCCCTATCACGTC
  CCTGAGGGAGATCAATACAATTTTGAAGGCCCAGCACCCGAACATTGTAACAGTGCGGGAGATCGTGGTG
  GGAAGTAATATGGATAAGATTTATATCGTCATGAACTACGTTGAACACGATCTGAAGAGCTTGATGGAAA
  CTATGAAGCAACCATTTCTGCCAGGAGAGGTGAAGACCCTGATGATACAACTGTTGAGAGGGGTGAAGCA
  CCTGCACGATAACTGGATCCTGCACAGAGATCTGAAAACCTCCAACCTCCTGCTTTCACATGCTGGTATC
  CTGAAAGTTGGGGATTTCGGACTGGCAAGGGAGTACGGATCACCTCTCAAGGCTTATACCCCGGTGGTGG
  TGACCCTCTGGTACCGGGCACCAGAACTGCTGTTGGGAGCTAAAGAGTATTCAACGGCCGTGGATATGTG
  GTCCGTCGGATGCATCTTCGGCGAACTCCTCACACAAAAACCCCTGTTCCCCGGGAAAAGTGAGATTGAT
  CAGATCAACAAAGTCTTCAAAGACCTGGGGACCCCCAGCGAAAAGATCTGGCCGGGGTATAGCGAACTGC
  CTGCTGTTAAAAAAATGACCTTCTCCGAGCATCCATACAATAACCTCCGGAAGAGGTTTGGGGCCCTGCT
  GAGTGATCAGGGCTTCGACCTGATGAATAAATTTCTTACTTACTTTCCCGGACGCCGCATTAGTGCCGAA
  GACGGCCTCAAGCACGAATACTTTCGCGAAACTCCACTGCCCATCGACCCTTCCATGTTTCCGACCTGGC
  CAGCAAAAAGCGAACAGCAAAGGGTGAAGAGAGGCACTTCTCCGCGCCCGCCGGAGGGGGGTCTTGGCTA
  CAGCCAACTGGGCGACGATGATCTGAAGGAGACAGGCTTCCATTTGACTACTACAAACCAGGGCGCTAGC
  GCTGCTGGACCAGGGTTCAGCCTCAAGTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215881 representing NM_033492
  Red=Cloning site Green=Tags(s)
  
  MGDEKDSWKVKTLDEILQEKKRRKEQEEKAEIKRLKNSDDRDSKRDSLEEGELRDHCMEITIRNSPYRRE
  DSMEDRGEEDDSLAIKPPQQMSRKEKVHHRKDEKRKEKKHARVKEKEREHERRKRHREEQDKARREWERQ
  KRREMAREHSRRERGNDGVCLFRDRLEQLERKRERERKMREQQKEQREQKERERRAEERRKEREARREVS
  AHHRTMREDYSDKVKASHWSRSPPRPPRERFELGDGRKPVKEEKMEERDLLSDLQDISDSERKTSSAESS
  SAESGSGSEEEEEEEEEEEEEGSTSEESEEEEEEEEEEEEETGSNSEEASEQSAEEVSEEEMSEDEEREN
  ENHLLVVPESRFDRDSGESEEAEEEVGEGTPQSSALTEGDYVPDSPALSPIELKQELPKYLPALQGCRSV
  EEFQCLNRIEEGTYGVVYRAKDKKTDEIVALKRLKMEKEKEGFPITSLREINTILKAQHPNIVTVREIVV
  GSNMDKIYIVMNYVEHDLKSLMETMKQPFLPGEVKTLMIQLLRGVKHLHDNWILHRDLKTSNLLLSHAGI
  LKVGDFGLAREYGSPLKAYTPVVVTLWYRAPELLLGAKEYSTAVDMWSVGCIFGELLTQKPLFPGKSEID
  QINKVFKDLGTPSEKIWPGYSELPAVKKMTFSEHPYNNLRKRFGALLSDQGFDLMNKFLTYFPGRRISAE
  DGLKHEYFRETPLPIDPSMFPTWPAKSEQQRVKRGTSPRPPEGGLGYSQLGDDDLKETGFHLTTTNQGAS
  AAGPGFSLKF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033492
• ORF Size: 2340 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033492.1, NP_277027.1
• RefSeq Size: 2480 bp
• RefSeq ORF: 2342 bp
• Locus ID: 984
• Cytogenetics: 1p36.33
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'This gene encodes a member of the serine/threonine protein kinase family. Members of this kinase family are known to be essential for eukaryotic cell cycle control. Due to a segmental duplication, this gene shares very high sequence identity with a neighboring gene. These two genes are frequently deleted or altered in neuroblastoma. The protein kinase encoded by this gene can be cleaved by caspases and may play a role in cell apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]'