HIF3 alpha (HIF3A) (NM_022462) Human Tagged ORF Clone

CAT#: RG215953

HIF3A (GFP-tagged) - Human hypoxia inducible factor 3, alpha subunit (HIF3A), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HIF3A
• Synonyms: bHLHe17; HIF-3A; HIF3-alpha-1; IPAS; MOP7; PASD7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215953 representing NM_022462
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCCTCACCATCAGCTACCTGCGCATGCACCGCCTCTGCGCCGCAGGGGAGTGGAACCAGGTGGGAG
  CAGGGGGAGAACCACTGGATGCCTGCTACCTGAAGGCCCTGGAGGGCTTCGTCATGGTGCTCACCGCCGA
  GGGAGACATGGCTTACCTGTCGGAGAATGTCAGCAAACACCTGGGCCTCAGTCAGCTGGAGCTCATTGGA
  CACAGCATCTTTGATTTCATCCACCCCTGTGACCAAGAGGAGCTTCAGGACGCCCTGACCCCCCAGCAGA
  CCCTGTCCAGGAGGAAGGTGGAGGCCCCCACGGAGCGGTGCTTCTCCTTGCGCATGAAGAGTACACTCAC
  CAGCCGCGGGCGCACCCTCAACCTCAAGGCGGCCACCTGGAAGGTGCTGAACTGCTCTGGACATATGAGG
  GCCTACAAGCCACCTGCGCAGACTTCTCCAGCTGGGAGCCCTGACTCAGAGCCCCCGCTGCAGTGCCTGG
  TGCTCATCTGCGAAGCCATCCCCCACCCAGGCAGCCTGGAGCCCCCACTGGGCCGAGGGGCCTTCCTCAG
  CCGCCACAGCCTGGACATGAAGTTCACCTACTGTGACGACAGGATTGCAGAAGTGGCTGGCTATAGTCCC
  GATGACCTGATCGGCTGTTCCGCCTACGAGTACATCCACGCGCTGGACTCCGATGCGGTCAGCAAGAGCA
  TCCACACCTTGCTGAGCAAGGGCCAGGCAGTAACAGGGCAGTATCGCTTCCTGGCCCGGAGTGGTGGCTA
  CCTGTGGACCCAGACCCAGGCCACAGTGGTGTCAGGGGGACGGGGCCCCCAGTCGGAGAGTATCGTCTGT
  GTCCATTTTTTAATCAGCCAGGTGGAAGAGACCGGAGTGGTGCTGTCCCTGGAGCAAACGGAGCAACACT
  CTCGCAGACCCATTCAGCGGGGCGCCCCCTCTCAGAAGGACACCCCTAACCCTGGGGACAGCCTTGACAC
  CCCTGGCCCCCGGATCCTTGCCTTCCTGCACCCGCCTTCCCTGAGCGAGGCTGCCCTGGCCGCTGACCCC
  CGCCGTTTCTGCAGCCCTGACCTCCGTCGCCTCCTGGGACCCATCCTGGATGGGGCTTCAGTAGCAGCCA
  CTCCCAGCACCCCGCTGGCCACACGGCACCCCCAAAGTCCTCTTTCGGCTGATCTCCCAGATGAACTACC
  TGTGGGCACCGAGAATGTGCACAGACTCTTCACCTCCGGGAAAGACACTGAGGCAGTGGAGACAGATTTA
  GATATAGCTCAGGATGCTGATGCTCTGGATTTGGAGATGCTGGCCCCCTACATCTCCATGGATGATGACT
  TCCAGCTCAACGCCAGCGAGCAGCTACCCAGGGCCTACCACAGACCTCTGGGGGCTGTCCCCCGGCCCCG
  TGCTCGGAGCTTCCATGGCCTGTCACCTCCAGCCCTTGAGCCCTCCCTGCTACCCCGCTGGGGGAGTGAC
  CCCCGGCTGAGCTGCTCCAGCCCTTCCAGAGGGGACCCCTCAGCATCCTCTCCCATGGCTGGGGCTCGGA
  AGAGGACCCTGGCCCAGAGCTCAGAGGACGAGGACGAGGGAGTGGAGCTGCTGGGAGTGAGACCTCCCAA
  AAGGTCCCCCAGCCCAGAACACGAAAACTTTCTGCTCTTTCCTCTCAGCCTGAGTTTCCTTCTGACAGGA
  GGACCAGCCCCAGGGAGCCTGCAGGACCCCACTGAACTTACCCAATTCCTTCTTTCAGTCTTAAGTTTTC
  CCATTCTAGACCCCTACCCTCTAGGCTGTGCTGCTCCTGGACTTCATGCCTCTCCATTCTCATTGCCTAC
  AATCTCTGTGCCCCAGAACCCCCTCCACTTCCCACCCCAGCCCTCCAGACATGCACTTACCTTGACTTTA
  CCCCACATGTTTGGGGCACCTGGGGCTCCCTCACCCCTTGGGTGGTTTGCAATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215953 representing NM_022462
  Red=Cloning site Green=Tags(s)
  
  MRLTISYLRMHRLCAAGEWNQVGAGGEPLDACYLKALEGFVMVLTAEGDMAYLSENVSKHLGLSQLELIG
  HSIFDFIHPCDQEELQDALTPQQTLSRRKVEAPTERCFSLRMKSTLTSRGRTLNLKAATWKVLNCSGHMR
  AYKPPAQTSPAGSPDSEPPLQCLVLICEAIPHPGSLEPPLGRGAFLSRHSLDMKFTYCDDRIAEVAGYSP
  DDLIGCSAYEYIHALDSDAVSKSIHTLLSKGQAVTGQYRFLARSGGYLWTQTQATVVSGGRGPQSESIVC
  VHFLISQVEETGVVLSLEQTEQHSRRPIQRGAPSQKDTPNPGDSLDTPGPRILAFLHPPSLSEAALAADP
  RRFCSPDLRRLLGPILDGASVAATPSTPLATRHPQSPLSADLPDELPVGTENVHRLFTSGKDTEAVETDL
  DIAQDADALDLEMLAPYISMDDDFQLNASEQLPRAYHRPLGAVPRPRARSFHGLSPPALEPSLLPRWGSD
  PRLSCSSPSRGDPSASSPMAGARKRTLAQSSEDEDEGVELLGVRPPKRSPSPEHENFLLFPLSLSFLLTG
  GPAPGSLQDPTELTQFLLSVLSFPILDPYPLGCAAPGLHASPFSLPTISVPQNPLHFPPQPSRHALTLTL
  PHMFGAPGAPSPLGWFAI
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022462
• ORF Size: 1944 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022462.2, NP_071907.2
• RefSeq Size: 2626
• RefSeq ORF: 1803
• Locus ID: 64344
• Domains: PAS
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]