Estrogen Related Receptor beta (ESRRB) (NM_004452) Human Tagged ORF Clone

CAT#: RG215995

ESRRB (GFP-tagged) - Human estrogen-related receptor beta (ESRRB)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ESRRB
• Synonyms: DFNB35; ERR2; ERRb; ESRL2; NR3B2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215995 representing NM_004452
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTCGGACGACAGGCACCTGGGCTCCAGCTGCGGCTCCTTCATCAAGACTGAGCCGTCCAGCCCGT
  CCTCGGGCATCGATGCCCTCAGCCACCACAGCCCCAGTGGCTCGTCCGACGCCAGCGGCGGCTTTGGCCT
  GGCCCTGGGCACCCACGCCAACGGTCTGGACTCGCCACCCATGTTTGCAGGCGCCGGGCTGGGAGGCACC
  CCATGCCGCAAGAGCTACGAGGACTGTGCCAGCGGCATCATGGAGGACTCGGCCATCAAGTGCGAGTACA
  TGCTCAACGCCATCCCCAAGCGCCTGTGCCTCGTGTGCGGGGACATTGCCTCTGGCTACCACTACGGCGT
  GGCCTCCTGCGAGGCTTGCAAGGCCTTCTTCAAGAGGACTATCCAAGGGAACATTGAGTACAGCTGCCCG
  GCCACCAACGAGTGCGAGATCACCAAACGGAGGCGCAAGTCCTGCCAGGCCTGCCGCTTCATGAAATGCC
  TCAAAGTGGGGATGCTGAAGGAAGGTGTGCGCCTTGATCGAGTGCGTGGAGGCCGTCAGAAATACAAGCG
  ACGGCTGGACTCAGAGAGCAGCCCATACCTGAGCTTACAAATTTCTCCACCTGCTAAAAAGCCATTGACC
  AAGATTGTCTCATACCTACTGGTGGCTGAGCCGGACAAGCTCTATGCCATGCCTCCCCCTGGTATGCCTG
  AGGGGGACATCAAGGCCCTGACCACTCTCTGTGACCTGGCAGACCGAGAGCTTGTGGTCATCATTGGCTG
  GGCCAAGCACATCCCAGGCTTCTCAAGCCTCTCCCTGGGGGACCAGATGAGCCTGCTGCAGAGTGCCTGG
  ATGGAAATCCTCATCCTGGGCATCGTGTACCGCTCGCTGCCCTACGACGACAAGCTGGTGTACGCTGAGG
  ACTACATCATGGATGAGGAGCACTCCCGCCTCGCGGGGCTGCTGGAGCTCTACCGGGCCATCCTGCAGCT
  GGTACGCAGGTACAAGAAGCTCAAGGTGGAGAAGGAGGAGTTTGTGACGCTCAAGGCCCTGGCCCTCGCC
  AACTCCGATTCCATGTACATCGAGGATCTAGAGGCTGTCCAGAAGCTGCAGGACCTGCTGCACGAGGCAC
  TGCAGGACTACGAGCTGAGCCAGCGCCATGAGGAGCCCTGGAGGACGGGCAAGCTGCTGCTGACACTGCC
  GCTGCTGCGGCAGACGGCCGCCAAGGCCGTGCAGCACTTCTATAGCGTCAAACTGCAGGGCAAAGTGCCC
  ATGCACAAACTCTTCCTGGAGATGCTGGAGGCCAAGGCCTGGGCCAGGGCTGACTCCCTTCAGGAGTGGA
  GGCCACTGGAGCAAGTGCCCTCTCCCCTCCACCGAGCCACCAAGAGGCAGCATGTGCATTTCCTAACTCC
  CTTGCCCCCTCCCCCATCTGTGGCCTGGGTGGGCACTGCTCAGGCTGGATACCACCTGGAGGTTTTCCTT
  CCGCAGAGGGCAGGTTGGCCAAGAGCAGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215995 representing NM_004452
  Red=Cloning site Green=Tags(s)
  
  MSSDDRHLGSSCGSFIKTEPSSPSSGIDALSHHSPSGSSDASGGFGLALGTHANGLDSPPMFAGAGLGGT
  PCRKSYEDCASGIMEDSAIKCEYMLNAIPKRLCLVCGDIASGYHYGVASCEACKAFFKRTIQGNIEYSCP
  ATNECEITKRRRKSCQACRFMKCLKVGMLKEGVRLDRVRGGRQKYKRRLDSESSPYLSLQISPPAKKPLT
  KIVSYLLVAEPDKLYAMPPPGMPEGDIKALTTLCDLADRELVVIIGWAKHIPGFSSLSLGDQMSLLQSAW
  MEILILGIVYRSLPYDDKLVYAEDYIMDEEHSRLAGLLELYRAILQLVRRYKKLKVEKEEFVTLKALALA
  NSDSMYIEDLEAVQKLQDLLHEALQDYELSQRHEEPWRTGKLLLTLPLLRQTAAKAVQHFYSVKLQGKVP
  MHKLFLEMLEAKAWARADSLQEWRPLEQVPSPLHRATKRQHVHFLTPLPPPPSVAWVGTAQAGYHLEVFL
  PQRAGWPRAA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004452
• ORF Size: 1500 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004452.2, NP_004443.2
• RefSeq Size: 2193 bp
• RefSeq ORF: 1527 bp
• Locus ID: 2103
• Cytogenetics: 14q24.3
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Gene Summary: 'This gene encodes a protein with similarity to the estrogen receptor. Its function is unknown; however, a similar protein in mouse plays an essential role in placental development. [provided by RefSeq, Jul 2008]'