ZNF121 (NM_001008727) Human Tagged ORF Clone

CAT#: RG216123

ZNF121 (GFP-tagged) - Human zinc finger protein 121 (ZNF121)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZNF121
• Synonyms: D19S204; ZHC32; ZNF20
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216123 representing NM_001008727
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAAATCCACAATGGAGGGGAACTCTGTGACTTTATGGAAAATGGAGAAATCTTCAGTGAACACT
  CATGCCTTAATGCACACATGGGAACTGAAAATACAGGGGACACTTATGACTGTGATGAGTATGGAGAAAA
  CTTTCCCATGTTACACAACAGTGCCCCTGCTGGAGAGACACTTTCTGTGTTGAATCAGTGCAGAAAAGCC
  TTCAGCCTGCCACCAAATGTTCACCAGAGAACGTGGATAGGAGACAAATCCTTTGAATACAGTGACTGTG
  AGGAAGCCTTTGTTGATCAGTCACATCTTCAGGCAAATAGGATAACTCACAATGGAGAAACACTCTATGA
  ACAGAAGCAATGTGGGAGAGCTTTTACTTACTCCACAAGCCATGCTGTGTCTGTTAAAATGCATACTGTA
  GAAAAACCCTACGAATGTAAGGAATGTGGAAAATTCTTTAGATATTCTTCATATCTTAATAGTCACATGC
  GAACCCATACTGGGGAGAAACCCTATGAATGTAAGGAATGTGGGAAATGCTTCACTGTTTCTTCACACCT
  AGTTGAACATGTAAGAATTCATACTGGAGAGAAACCTTATCAATGTAAGGAATGTGGAAGAGCCTTCGCT
  GGGCGCTCAGGCCTTACTAAACATGTACGAATACACACTGGAGAGAAGCCCTATGAATGTAACGAATGTG
  GGAAAGCCTACAATAGGTTTTATCTACTAACTGAACATTTTAAAACTCACACAGAGGAGAAGCCCTTTGA
  ATGTAAGGTATGTGGAAAATCCTTCAGAAGCTCTTCATGCCTTAAGAATCACTTTAGAATTCACACTGGA
  ATAAAACCCTATAAATGTAAGGAGTGTGGGAAAGCATTCACTGTTTCCTCAAGCTTACATAATCATGTAA
  AAATCCATACTGGAGAGAAGCCCTATGAATGTAAGGACTGTGGGAAAGCCTTTGCTACATCTTCACAACT
  CATTGAACATATAAGAACTCACACTGGAGAGAAACCGTATATATGTAAGGAATGTGGGAAAACCTTCCGT
  GCTTCTTCACATCTACAGAAACATGTTAGAATTCACACTGGAGAGAAACCCTATATATGTAACGAATGTG
  GGAAAGCCTACAATAGATTTTATTTACTTACTAAACATTTAAAAACACAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216123 representing NM_001008727
  Red=Cloning site Green=Tags(s)
  
  MAEIHNGGELCDFMENGEIFSEHSCLNAHMGTENTGDTYDCDEYGENFPMLHNSAPAGETLSVLNQCRKA
  FSLPPNVHQRTWIGDKSFEYSDCEEAFVDQSHLQANRITHNGETLYEQKQCGRAFTYSTSHAVSVKMHTV
  EKPYECKECGKFFRYSSYLNSHMRTHTGEKPYECKECGKCFTVSSHLVEHVRIHTGEKPYQCKECGRAFA
  GRSGLTKHVRIHTGEKPYECNECGKAYNRFYLLTEHFKTHTEEKPFECKVCGKSFRSSSCLKNHFRIHTG
  IKPYKCKECGKAFTVSSSLHNHVKIHTGEKPYECKDCGKAFATSSQLIEHIRTHTGEKPYICKECGKTFR
  ASSHLQKHVRIHTGEKPYICNECGKAYNRFYLLTKHLKTH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001008727
• ORF Size: 1170 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008727.1, NP_001008727.1
• RefSeq Size: 1735 bp
• RefSeq ORF: 1173 bp
• Locus ID: 7675
• Cytogenetics: 19p13.2
• Gene Summary: ''