MUC4 (NM_004532) Human Tagged ORF Clone

CAT#: RG216276

MUC4 (GFP-tagged) - Human mucin 4, cell surface associated (MUC4), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MUC4
• Synonyms: ASGP; HSA276359; MUC-4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216276 representing NM_004532
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGGGGCACGCTGGAGGAGGGTCCCCTGGGTGTCCCTGAGCTGCCTGTGTCTCTGCCTCCTTCCGC
  ATGTGGTCCCAGGAATGACAACACCGTCACTGAAGACAGACGGTGGGAGACGCACAGCCACATCACCACC
  CCCCACAACCTCCCAGACCATCATTTCCACCATTCCCAGCACTGCCATGCACACCCGCTCCACAGCTGCC
  CCCATCCCCATCCTGCCTGAGAGAGGAGTTTCCCTCTTCCCCTATGGGGCAGACGCCGGGGACCTGGAGT
  TCGTCAGGAGGACCGTGGACTTCACCTCCCCACTCTTCAAGCCGGCGACTGGCTTCCCCCTTGGCTCCTC
  TCTCCGTGATTCCCTCTACTTCACAGACAATGGCCAGATCATCTTCCCAGAGTCAGACTACCAGATTTTC
  TCCTACCCCAACCCACTCCCAACAGGCTTCACAGGCCGGGACCCTGTGGCCCTGGTGGCTCCGTTCTGGG
  ACGATGCTGACTTCTCCACTGGTCGGGGGACCACATTTTATCAGGAATACGAGACGTTCTATGGTGAACA
  CAGCCTGCTAGTCCAGCAGGCCGAGTCTTGGATTAGAAAGATCACAAACAACGGGGGCTACAAGGCCAGG
  TGGGCCCTAAAGGTCACGTGGGTCAATGCCCACGCCTATCCTGCCCAGTGGACCCTCGGGAGCAACACCT
  ACCAAGCCATCCTCTCCACGGACGGGAGCAGGTCCTATGCCCTGTTTCTCTACCAGAGCGGTGGGATGCA
  GTGGGACGTGGCCCAGCGCTCAGGCAAGCCGGTGCTCATGGGCTTCTCTAGTGGAGATGGCTATTTCGAA
  AACAGCCCACTGATGTCCCAGCCAGTGTGGGAGAGGTATCGCCCTGATAGATTCCTGAATTCCAACTCAG
  GCCTCCAAGGGCTGCAGTTCTACAGGCTACACCGGGAAGAAAGGCCCAACTACCGTCTCGAGTGCCTGCA
  GTGGCTGAAGAGCCAGCCTCGGTGGCCCAGCTGGGGCTGGAACCAGGTCTCCTGCCCTTGTTCCTGGCAG
  CAGGGACGACGGGACTTACGATTCCAACCCGTCAGCATAGGTCGCTGGGGCCTCGGCAGTAGGCAGCTGT
  GCAGCTTCACCTCTTGGCGAGGAGGCGTGTGCTGCAGCTACGGGCCCTGGGGAGAGTTTCGTGAAGGCTG
  GCACGTGCAGCGTCCTTGGCAGTTGGCCCAGGAACTGGAGCCACAGAGCTGGTGCTGCCGCTGGAATGAC
  AAGCCCTACCTCTGTGCCCTGTACCAGCAGAGGCGGCCCCACGTGGGCTGTGCTACATACAGGCCCCCAC
  AGCCCGCCTGGATGTTCGGGGACCCCCACATCACCACCTTGGATGGTGTCAGTTACACCTTCAATGGGCT
  GGGGGACTTCCTGCTGGTCGGGGCCCAAGACGGGAACTCCTCCTTCCTGCTTCAGGGCCGCACCGCCCAG
  ACTGGCTCAGCCCAGGCCACCAACTTCATCGCCTTTGCGGCTCAGTACCGCTCCAGCAGCCTGGGCCCCG
  TCACGGTCCAATGGCTCCTTGAGCCTCACGACGCAATCCGTGTCCTGCTGGATAACCAGACTGTGACATT
  TCAGCCTGACCATGAAGACGGCGGAGGCCAGGAGACGTTCAACGCCACCGGAGTCCTCCTGAGCCGCAAC
  GGCTCTGAGGTCTCGGCCAGCTTCGACGGCTGGGCCACCGTCTCGGTGATCGCGCTCTCCAACATCCTCC
  ACGCCTCCGCCAGCCTCCCGCCCGAGTACCAGAACCGCACGGAGGGGCTCCTGGGGGTCTGGAATAACAA
  TCCAGAGGACGACTTCAGGATGCCCAATGGCTCCACCATTCCCCCAGGGAGCCCTGAGGAGATGCTTTTC
  CACTTTGGAATGACCTGGCAGATCAACGGGACAGGCCTCCTTGGCAAGAGGAATGACCAGCTGCCTTCCA
  ACTTCACCCCTGTTTTCTACTCACAACTGCAAAAAAACAGCTCCTGGGCTGAACATTTGATCTCCAACTG
  TGACGGAGATAGCTCATGCATCTATGACACCCTGGCCCTGCGCAACGCAAGCATCGGACTTCACACGAGG
  GAAGTCAGTAAAAACTACGAGCAGGCGAACGCCACCCTCAATCAGTACCCGCCCTCCATCAATGGTGGTC
  GTGTGATTGAAGCCTACAAGGGGCAGACCACGCTGATTCAGTACACCAGCAATGCTGAGGATGCCAACTT
  CACGCTCAGAGACAGCTGCACCGACTTGGAGCTCTTTGAGAATGGGACGTTGCTGTGGACACCCAAGTCG
  CTGGAGCCATTCACTCTGGAGATTCTAGCAAGAAGTGCCAAGATTGGCTTGGCATCTGCACTCCAGCCCA
  GGACTGTGGTCTGCCATTGCAATGCAGAGAGCCAGTGTTTGTACAATCAGACCAGCAGGGTGGGCAACTC
  CTCCCTGGAGGTGGCTGGCTGCAAGTGTGACGGGGGCACCTTCGGCCGCTACTGCGAGGGCTCCGAGGAT
  GCCTGTGAGGAGCCGTGCTTCCCGAGTGTCCACTGCGTTCCTGGGAAGGGCTGCGAGGCCTGCCCTCCAA
  ACCTGACTGGGGATGGGCGGCACTGTGCGGCTCTGGGGAGCTCTTTCCTGTGTCAGAACCAGTCCTGCCC
  TGTGAATTACTGCTACAATCAAGGCCACTGCTACATCTCCCAGACTCTGGGCTGTCAGCCCATGTGCACC
  TGCCCCCCAGCCTTCACTGACAGCCGCTGCTTCCTGGCTGGGAACAACTTCAGTCCAACTGTCAACCTAG
  AACTTCCCTTAAGAGTCATCCAGCTCTTGCTCAGTGAAGAGGAAAATGCCTCCATGGCAGAGGTCAACGC
  CTCGGTGGCATACAGACTGGGGACCCTGGACATGCGGGCCTTTCTCCGCAACAGCCAAGTGGAACGAATC
  GATTCTGCAGCACCGGCCTCGGGAAGCCCCATCCAACACTGGATGGTCATCTCGGAGTTCCAGTACCGCC
  CTCGGGGCCCGGTCATTGACTTCCTGAACAACCAGCTGCTGGCCGCGGTGGTGGAGGCGTTCTTATACCA
  CGTTCCACGGAGGAGTGAGGAGCCCAGGAACGACGTGGTCTTCCAGCCCATCTCCGGGGAAGACGTGCGC
  GATGTGACAGCCCTGAACGTGAGCACGCTGAAGGCTTACTTCAGATGCGATGGCTACAAGGGCTACGACC
  TGGTCTACAGCCCCCAGAGCGGCTTCACCTGCGTGTCCCCGTGCAGTAGGGGCTACTGTGACCATGGAGG
  CCAGTGCCAGCACCTGCCCAGTGGGCCCCGCTGCAGCTGTGTGTCCTTCTCCATCTACACGGCCTGGGGC
  GAGCACTGTGAGCACCTGAGCATGAAACTCGACGCGTTCTTCGGCATCTTCTTTGGGGCCCTGGGCGGCC
  TCTTGCTGCTGGGGGTCGGGACGTTCGTGGTCCTGCGCTTCTGGGGTTGCTCCGGGGCCAGGTTCTCCTA
  TTTCCTGAACTCAGCTGAGGCCTTGCCT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216276 representing NM_004532
  Red=Cloning site Green=Tags(s)
  
  MKGARWRRVPWVSLSCLCLCLLPHVVPGMTTPSLKTDGGRRTATSPPPTTSQTIISTIPSTAMHTRSTAA
  PIPILPERGVSLFPYGADAGDLEFVRRTVDFTSPLFKPATGFPLGSSLRDSLYFTDNGQIIFPESDYQIF
  SYPNPLPTGFTGRDPVALVAPFWDDADFSTGRGTTFYQEYETFYGEHSLLVQQAESWIRKITNNGGYKAR
  WALKVTWVNAHAYPAQWTLGSNTYQAILSTDGSRSYALFLYQSGGMQWDVAQRSGKPVLMGFSSGDGYFE
  NSPLMSQPVWERYRPDRFLNSNSGLQGLQFYRLHREERPNYRLECLQWLKSQPRWPSWGWNQVSCPCSWQ
  QGRRDLRFQPVSIGRWGLGSRQLCSFTSWRGGVCCSYGPWGEFREGWHVQRPWQLAQELEPQSWCCRWND
  KPYLCALYQQRRPHVGCATYRPPQPAWMFGDPHITTLDGVSYTFNGLGDFLLVGAQDGNSSFLLQGRTAQ
  TGSAQATNFIAFAAQYRSSSLGPVTVQWLLEPHDAIRVLLDNQTVTFQPDHEDGGGQETFNATGVLLSRN
  GSEVSASFDGWATVSVIALSNILHASASLPPEYQNRTEGLLGVWNNNPEDDFRMPNGSTIPPGSPEEMLF
  HFGMTWQINGTGLLGKRNDQLPSNFTPVFYSQLQKNSSWAEHLISNCDGDSSCIYDTLALRNASIGLHTR
  EVSKNYEQANATLNQYPPSINGGRVIEAYKGQTTLIQYTSNAEDANFTLRDSCTDLELFENGTLLWTPKS
  LEPFTLEILARSAKIGLASALQPRTVVCHCNAESQCLYNQTSRVGNSSLEVAGCKCDGGTFGRYCEGSED
  ACEEPCFPSVHCVPGKGCEACPPNLTGDGRHCAALGSSFLCQNQSCPVNYCYNQGHCYISQTLGCQPMCT
  CPPAFTDSRCFLAGNNFSPTVNLELPLRVIQLLLSEEENASMAEVNASVAYRLGTLDMRAFLRNSQVERI
  DSAAPASGSPIQHWMVISEFQYRPRGPVIDFLNNQLLAAVVEAFLYHVPRRSEEPRNDVVFQPISGEDVR
  DVTALNVSTLKAYFRCDGYKGYDLVYSPQSGFTCVSPCSRGYCDHGGQCQHLPSGPRCSCVSFSIYTAWG
  EHCEHLSMKLDAFFGIFFGALGGLLLLGVGTFVVLRFWGCSGARFSYFLNSAEALP
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004532
• ORF Size: 3528 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_004532.2, NP_004523.2
• RefSeq Size: 4411
• RefSeq ORF: 3531
• Locus ID: 4585
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface, although secreted isoforms may exist. At least two dozen transcript variants of this gene have been found, although for many of them the full-length transcript has not been determined or they are found only in tumor tissues. This gene contains a region in the coding sequence which has a variable number (>100) of 48 nt tandem repeats. [provided by RefSeq, Jul 2008]