MGAM (NM_004668) Human Tagged ORF Clone

CAT#: RG216305

MGAM (GFP-tagged) - Human maltase-glucoamylase (alpha-glucosidase) (MGAM). Note: ORF is codon optimized

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MGAM
• Synonyms: MG; MGA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216305 ORF sequence, codon optimized.
  Due to the complexity of NM_004668, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAGAAAGAAGCTGAAAAAATTTACTACTTTGGAGATTGTGCTCAGTGTTCTTCTGCTTGTGTTGT
  TTATCATCAGTATTGTTCTAATTGTGCTTTTAGCCAAAGAGTCACTGAAATCAACAGCCCCAGATCCTGG
  GACAACTGGTACCCCAGATCCTGGGACAACTGGTACCCCAGATCCTGGAACAACTGGTACCACACATGCT
  AGGACAACGGGTCCCCCAGATCCTGGAACAACTGGTACCACTCCTGTTTCTGCTGAATGTCCAGTGGTAA
  ATGAATTGGAACGAATTAATTGCATCCCTGACCAGCCGCCAACAAAGGCCACATGTGACCAACGTGGCTG
  TTGCTGGAATCCCCAGGGAGCTGTAAGTGTTCCCTGGTGCTACTATTCCAAGAATCATAGCTACCATGTA
  GAGGGCAACCTTGTCAACACAAATGCAGGATTCACAGCCCGGTTGAAAAATCTGCCTTCTTCACCAGTGT
  TTGGCAGCAACGTCGATAACGTCCTCCTGACCGCTGAATATCAGACATCTAACAGATTTCATTTCAAACT
  CACCGACCAAACCAATAATCGCTTCGAGGTGCCCCACGAACACGTCCAATCATTTAGCGGCAATGCCGCA
  GCATCTCTGACTTACCAGGTGGAGATTTCCAGACAGCCTTTTAGTATCAAAGTGACCCGGCGCAGTAATA
  ACCGAGTTCTCTTCGATTCTTCCATTGGGCCTTTGTTGTTTGCAGATCAGTTCCTCCAACTGTCCACCCG
  CCTGCCTAGTACTAACGTGTATGGCCTCGGAGAGCACGTGCACCAGCAATATCGCCACGATATGAATTGG
  AAAACTTGGCCCATCTTCAACCGCGACACCACACCTAACGGAAATGGCACCAATCTGTATGGTGCACAAA
  CATTCTTCCTCTGTCTGGAAGATGCATCCGGGTTGTCATTTGGCGTATTTCTGATGAATTCTAACGCAAT
  GGAGGTCGTGCTCCAGCCGGCCCCAGCCATCACATACAGAACAATCGGAGGAATCCTGGATTTCTACGTG
  TTCCTCGGGAACACACCAGAGCAGGTCGTGCAAGAGTATTTGGAACTCATTGGTAGGCCTGCCCTGCCCT
  CTTACTGGGCTCTGGGTTTTCATCTTTCACGATACGAATATGGCACCCTGGACAACATGAGGGAGGTCGT
  CGAACGCAACCGAGCCGCCCAACTGCCGTACGACGTGCAGCACGCTGATATAGATTACATGGATGAACGG
  CGGGACTTTACATATGACTCTGTGGATTTCAAAGGCTTCCCAGAATTCGTGAACGAACTTCATAATAATG
  GCCAGAAACTGGTAATCATCGTGGACCCTGCTATATCCAACAACTCTTCCTCAAGTAAACCCTATGGCCC
  ATATGACAGGGGTTCAGATATGAAGATATGGGTGAATAGTTCAGATGGAGTGACTCCACTCATTGGGGAG
  GTCTGGCCTGGACAAACTGTGTTTCCTGATTATACCAATCCCAACTGTGCTGTTTGGTGGACAAAGGAAT
  TTGAGCTTTTTCACAATCAAGTAGAGTTTGATGGAATCTGGATTGATATGAATGAAGTCTCCAACTTTGT
  TGATGGTTCGGTCTCAGGATGTTCCACAAACAACCTAAATAATCCCCCATTCACTCCCAGAATCCTGGAT
  GGGTACCTGTTCTGCAAGACTCTCTGTATGGATGCAGTGCAGCACTGGGGCAAGCAGTATGACATTCACA
  ATCTGTATGGCTACTCCATGGCGGTCGCCACAGCAGAAGCTGCCAAGACTGTGTTCCCTAATAAGAGAAG
  CTTCATTCTGACCCGTTCTACCTTTGCGGGCTCTGGCAAGTTTGCAGCACATTGGTTAGGAGACAACACT
  GCCACCTGGGATGACCTGAGATGGTCCATCCCTGGCGTGCTTGAGTTCAACCTTTTTGGCATCCCAATGG
  TGGGTCCTGACATATGTGGCTTTGCTTTGGACACCCCTGAGGAGCTCTGTAGGCGGTGGATGCAGTTGGG
  TGCATTTTATCCGTTTTCTAGAAATCACAATGGCCAAGGCTACAAGGACCAGGATCCTGCCTCCTTTGGA
  GCTGACTCCCTGCTGTTGAATTCCTCCAGGCACTACCTTAACATCCGCTATACTCTATTGCCCTACCTAT
  ACACCCTCTTCTTCCGTGCTCACAGCCGAGGGGACACGGTGGCCAGGCCCCTTTTGCATGAGTTCTACGA
  GGACAACAGCACTTGGGATGTGCACCAACAGTTCTTATGGGGGCCCGGCCTCCTCATCACTCCAGTTCTG
  GATGAAGGTGCAGAGAAAGTGATGGCATATGTGCCTGATGCTGTCTGGTATGACTACGAGACTGGGAGCC
  AAGTGAGATGGAGGAAGCAAAAAGTCGAGATGGAACTTCCTGGAGACAAAATTGGACTTCACCTTCGAGG
  AGGCTACATCTTCCCCACACAGCAGCCAAATACAACCACTCTGGCCAGTCGAAAGAACCCTCTTGGTCTT
  ATCATTGCCCTAGATGAGAACAAAGAAGCAAAAGGAGAACTTTTCTGGGATAATGGGGAAACGAAGGATA
  CTGTGGCCAATAAAGTGTATCTTTTATGTGAGTTTTCTGTCACTCAAAACCGCTTGGAGGTGAATATTTC
  ACAATCAACCTACAAGGACCCCAATAATTTAGCATTTAATGAGATTAAAATTCTTGGGACGGAGGAACCT
  AGCAATGTTACAGTGAAACACAATGGTGTCCCAAGTCAGACTTCTCCTACAGTCACTTATGATTCTAACC
  TGAAGGTTGCCATTATCACAGATATTGATCTTCTCCTGGGAGAAGCATACACAGTGGAATGGAGCATAAA
  GATAAGGGATGAAGAAAAAATAGACTGTTACCCTGATGAGAATGGTGCTTCTGCCGAAAACTGCACTGCC
  CGTGGCTGTATCTGGGAGGCATCCAATTCTTCTGGAGTCCCTTTTTGCTATTTTGTCAACGACCTATACT
  CTGTCAGTGATGTTCAGTATAATTCCCATGGGGCCACAGCTGACATCTCCTTAAAGTCTTCCGTTTATGC
  CAATGCCTTCCCCTCCACACCCGTGAACCCCCTTCGCCTGGATGTCACTTACCATAAGAATGAAATGCTG
  CAGTTCAAGATTTATGATCCCAACAAGAATCGGTATGAAGTTCCAGTCCCTCTGAACATACCCAGCATGC
  CATCCAGCACCCCTGAGGGTCAACTCTATGATGTGCTCATTAAGAAGAATCCATTTGGGATTGAAATTCG
  CCGGAAGAGTACAGGCACTATAATTTGGGACTCTCAGCTCCTTGGCTTTACCTTCAGTGACATGTTTATC
  CGCATCTCCACCCGCCTTCCCTCCAAGTACCTCTATGGCTTTGGGGAAACTGAGCACAGGTCCTATAGGA
  GAGACTTGGAGTGGCACACTTGGGGGATGTTCTCCCGAGACCAGCCCCCAGGGTACAAGAAGAATTCCTA
  TGGTGTCCACCCCTACTACATGGGGCTGGAGGAGGACGGCAGTGCCCATGGAGTGCTCCTGCTGAACAGC
  AATGCCATGGATGTGACGTTCCAGCCCCTGCCTGCCTTGACATACCGCACCACAGGGGGAGTTCTGGACT
  TTTATGTGTTCTTGGGGCCGACTCCAGAGCTTGTCACCCAGCAGTACACTGAGTTGATTGGCCGGCCTGT
  GATGGTACCTTACTGGTCTTTGGGGTTCCAGCTGTGTCGCTATGGCTACCAGAATGACTCTGAGATCGCC
  AGCTTGTATGATGAGATGGTGGCTGCCCAGATCCCTTATGATGTGCAGTACTCAGACATCGACTACATGG
  AGCGGCAGCTGGACTTCACCCTCAGCCCCAAGTTTGCTGGGTTTCCAGCTCTGATCAATCGCATGAAGGC
  TGATGGGATGCGGGTCATCCTCATTCTGGATCCAGCCATTTCTGGCAATGAGACACAGCCTTATCCTGCC
  TTCACTCGGGGCGTGGAGGATGACGTCTTCATCAAATACCCAAATGATGGAGACATTGTCTGGGGAAAGG
  TCTGGCCTGATTTTCCTGATGTTGTTGTGAATGGGTCTCTAGACTGGGACAGCCAAGTGGAGCTATATCG
  AGCTTATGTGGCCTTCCCAGACTTTTTCCGTAATTCAACTGCCAAGTGGTGGAAGAGGGAAATAGAAGAA
  CTATACAACAATCCACAGAATCCAGAGAGGAGCTTGAAGTTTGATGGCATGTGGATTGATATGAATGAAC
  CATCAAGCTTCGTGAATGGGGCAGTTTCTCCAGGCTGCAGGGACGCCTCTCTGAACCACCCTCCCTACAT
  GCCACATTTGGAGTCCAGGGACAGGGGCCTGAGCAGCAAGACCCTTTGTATGGAGAGTCAGCAGATCCTC
  CCAGACGGCTCCCTGGTGCAGCACTACAACGTGCACAACCTGTATGGGTGGTCCCAGACCAGACCCACAT
  ACGAAGCCGTGCAGGAGGTGACGGGACAGCGAGGGGTCGTCATCACCCGCTCCACATTTCCCTCTTCTGG
  CCGCTGGGCAGGACATTGGCTGGGAGACAACACGGCCGCATGGGATCAGCTGAAGAAGTCTATCATTGGC
  ATGATGGAGTTCAGCCTCTTCGGCATATCCTATACGGGAGCAGATATCTGTGGGTTCTTTCAAGATGCTG
  AATATGAGATGTGTGTTCGCTGGATGCAGCTGGGGGCCTTTTACCCCTTCTCAAGAAACCACAACACCAT
  TGGGACCAGGAGACAAGACCCTGTGTCCTGGGATGTTGCTTTTGTGAATATTTCCAGAACTGTCCTGCAG
  ACCAGATACACCCTGTTGCCATATCTGTATACCTTGATGCATAAGGCCCACACGGAGGGCGTCACTGTTG
  TGCGGCCTCTGCTCCATGAGTTTGTGTCAGACCAGGTGACATGGGACATAGACAGTCAGTTCCTGCTGGG
  CCCAGCCTTCCTGGTCAGCCCTGTCCTGGAGCGTAATGCCAGAAATGTCACTGCATATTTCCCTAGAGCC
  CGCTGGTATGATTACTACACGGGTGTGGATATTAATGCAAGAGGAGAGTGGAAGACCTTGCCAGCCCCTC
  TTGACCACATTAATCTTCATGTCCGTGGGGGCTACATCCTGCCCTGGCAAGAGCCTGCACTGAACACCCA
  CTTAAGCCGCCAGAAATTCATGGGCTTCAAAATTGCCTTGGATGATGAAGGAACTGCTGGGGGCTGGCTC
  TTCTGGGATGATGGGCAAAGCATTGATACCTATGGGAAAGGACTCTATTACTTGGCCAGCTTTTCTGCCA
  GCCAGAATACGATGCAAAGCCATATAATTTTCAACAATTACATCACTGGTACAAATCCTTTGAAACTGGG
  CTACATTGAAATCTGGGGAGTGGGCAGTGTCCCCGTTACCAGTGTCAGCATCTCTGTGAGTGGCATGGTC
  ATAACACCCTCCTTCAACAATGACCCCACGACACAGGTATTAAGCATCGATGTGACTGACAGAAACATCA
  GCCTACATAATTTTACTTCATTGACGTGGATAAGCACTCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216305 representing NM_004668
  Red=Cloning site Green=Tags(s)
  
  MARKKLKKFTTLEIVLSVLLLVLFIISIVLIVLLAKESLKSTAPDPGTTGTPDPGTTGTPDPGTTGTTHA
  RTTGPPDPGTTGTTPVSAECPVVNELERINCIPDQPPTKATCDQRGCCWNPQGAVSVPWCYYSKNHSYHV
  EGNLVNTNAGFTARLKNLPSSPVFGSNVDNVLLTAEYQTSNRFHFKLTDQTNNRFEVPHEHVQSFSGNAA
  ASLTYQVEISRQPFSIKVTRRSNNRVLFDSSIGPLLFADQFLQLSTRLPSTNVYGLGEHVHQQYRHDMNW
  KTWPIFNRDTTPNGNGTNLYGAQTFFLCLEDASGLSFGVFLMNSNAMEVVLQPAPAITYRTIGGILDFYV
  FLGNTPEQVVQEYLELIGRPALPSYWALGFHLSRYEYGTLDNMREVVERNRAAQLPYDVQHADIDYMDER
  RDFTYDSVDFKGFPEFVNELHNNGQKLVIIVDPAISNNSSSSKPYGPYDRGSDMKIWVNSSDGVTPLIGE
  VWPGQTVFPDYTNPNCAVWWTKEFELFHNQVEFDGIWIDMNEVSNFVDGSVSGCSTNNLNNPPFTPRILD
  GYLFCKTLCMDAVQHWGKQYDIHNLYGYSMAVATAEAAKTVFPNKRSFILTRSTFAGSGKFAAHWLGDNT
  ATWDDLRWSIPGVLEFNLFGIPMVGPDICGFALDTPEELCRRWMQLGAFYPFSRNHNGQGYKDQDPASFG
  ADSLLLNSSRHYLNIRYTLLPYLYTLFFRAHSRGDTVARPLLHEFYEDNSTWDVHQQFLWGPGLLITPVL
  DEGAEKVMAYVPDAVWYDYETGSQVRWRKQKVEMELPGDKIGLHLRGGYIFPTQQPNTTTLASRKNPLGL
  IIALDENKEAKGELFWDNGETKDTVANKVYLLCEFSVTQNRLEVNISQSTYKDPNNLAFNEIKILGTEEP
  SNVTVKHNGVPSQTSPTVTYDSNLKVAIITDIDLLLGEAYTVEWSIKIRDEEKIDCYPDENGASAENCTA
  RGCIWEASNSSGVPFCYFVNDLYSVSDVQYNSHGATADISLKSSVYANAFPSTPVNPLRLDVTYHKNEML
  QFKIYDPNKNRYEVPVPLNIPSMPSSTPEGQLYDVLIKKNPFGIEIRRKSTGTIIWDSQLLGFTFSDMFI
  RISTRLPSKYLYGFGETEHRSYRRDLEWHTWGMFSRDQPPGYKKNSYGVHPYYMGLEEDGSAHGVLLLNS
  NAMDVTFQPLPALTYRTTGGVLDFYVFLGPTPELVTQQYTELIGRPVMVPYWSLGFQLCRYGYQNDSEIA
  SLYDEMVAAQIPYDVQYSDIDYMERQLDFTLSPKFAGFPALINRMKADGMRVILILDPAISGNETQPYPA
  FTRGVEDDVFIKYPNDGDIVWGKVWPDFPDVVVNGSLDWDSQVELYRAYVAFPDFFRNSTAKWWKREIEE
  LYNNPQNPERSLKFDGMWIDMNEPSSFVNGAVSPGCRDASLNHPPYMPHLESRDRGLSSKTLCMESQQIL
  PDGSLVQHYNVHNLYGWSQTRPTYEAVQEVTGQRGVVITRSTFPSSGRWAGHWLGDNTAAWDQLKKSIIG
  MMEFSLFGISYTGADICGFFQDAEYEMCVRWMQLGAFYPFSRNHNTIGTRRQDPVSWDVAFVNISRTVLQ
  TRYTLLPYLYTLMHKAHTEGVTVVRPLLHEFVSDQVTWDIDSQFLLGPAFLVSPVLERNARNVTAYFPRA
  RWYDYYTGVDINARGEWKTLPAPLDHINLHVRGGYILPWQEPALNTHLSRQKFMGFKIALDDEGTAGGWL
  FWDDGQSIDTYGKGLYYLASFSASQNTMQSHIIFNNYITGTNPLKLGYIEIWGVGSVPVTSVSISVSGMV
  ITPSFNNDPTTQVLSIDVTDRNISLHNFTSLTWISTL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004668
• ORF Size: 5571 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_004668.2, NP_004659.2
• RefSeq Size: 6513
• RefSeq ORF: 5574
• Locus ID: 8972
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Galactose metabolism, Metabolic pathways, Starch and sucrose metabolism
• Gene Summary: This gene encodes maltase-glucoamylase, which is a brush border membrane enzyme that plays a role in the final steps of digestion of starch. The protein has two catalytic sites identical to those of sucrase-isomaltase, but the proteins are only 59% homologous. Both are members of glycosyl hydrolase family 31, which has a variety of substrate specificities. [provided by RefSeq, Jul 2008]