GHRH (NM_021081) Human Tagged ORF Clone
CAT#: RG216358
- TrueORF®
GHRH (GFP-tagged) - Human growth hormone releasing hormone (GHRH), transcript variant 1
"NM_021081" in other vectors (4)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | GHRH |
Synonyms | GHRF; GRF; INN |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG216358 representing NM_021081
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCACTCTGGGTGTTCTTCTTTGTGATCCTCACCCTCAGCAACAGCTCCCACTGCTCCCCACCTCCCC CTTTGACCCTCAGGATGCGGCGGTATGCAGATGCCATCTTCACCAACAGCTACCGGAAGGTGCTGGGCCA GCTGTCCGCCCGCAAGCTGCTCCAGGACATCATGAGCAGGCAGCAGGGAGAGAGCAACCAAGAGCGAGGA GCAAGGGCACGGCTTGGTCGTCAGGTAGACAGCATGTGGGCAGAACAAAAGCAAATGGAATTGGAGAGCA TCCTGGTGGCCCTGCTGCAGAAGCACAGCAGGAACTCCCAGGGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG216358 representing NM_021081
Red=Cloning site Green=Tags(s) MPLWVFFFVILTLSNSSHCSPPPPLTLRMRRYADAIFTNSYRKVLGQLSARKLLQDIMSRQQGESNQERG ARARLGRQVDSMWAEQKQMELESILVALLQKHSRNSQG TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_021081 |
ORF Size | 324 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021081.3, NP_066567.1 |
RefSeq Size | 454 bp |
RefSeq ORF | 327 bp |
Locus ID | 2691 |
Cytogenetics | 20q11.23 |
Protein Families | Druggable Genome, Secreted Protein |
Gene Summary | 'This gene encodes a member of the glucagon family of proteins. The encoded preproprotein is produced in the hypothalamus and cleaved to generate the mature factor, known as somatoliberin, which acts to stimulate growth hormone release from the pituitary gland. Variant receptors for somatoliberin have been found in several types of tumors, and antagonists of these receptors can inhibit the growth of the tumors. Defects in this gene are a cause of dwarfism, while hypersecretion of the encoded protein is a cause of gigantism. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC304905 | GHRH (untagged)-Human growth hormone releasing hormone (GHRH), transcript variant 1 |
USD 420.00 |
|
RC216358 | GHRH (Myc-DDK-tagged)-Human growth hormone releasing hormone (GHRH), transcript variant 1 |
USD 98.00 |
|
RC216358L3 | Lenti ORF clone of Human growth hormone releasing hormone (GHRH), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC216358L4 | Lenti ORF clone of Human growth hormone releasing hormone (GHRH), transcript variant 1, mGFP tagged |
USD 620.00 |
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