LRPPRC (NM_133259) Human Tagged ORF Clone

CAT#: RG216747

LRPPRC (GFP-tagged) - Human leucine-rich PPR-motif containing (LRPPRC)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LRPPRC
• Synonyms: CLONE-23970; GP130; LRP130; LSFC
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216747 representing NM_133259
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCCTGCTGAGATCCGCGCGTTGGTTGCTGCGTGCCGGGGCGGCCCCGCGCCTCCCGCTCTCCC
  TGCGCCTCCTCCCTGGCGGCCCGGGCCGGCTGCATGCCGCCTCCTATCTGCCCGCCGCTCGCGCCGGGCC
  CGTGGCCGGAGGACTACTGAGCCCAGCCAGGCTGTATGCCATTGCTGCCAAAGAAAAAGATATTCAAGAG
  GAGTCCACTTTTTCTTCTAGGAAGATTTCCAATCAGTTTGATTGGGCTCTAATGAGACTAGATCTTTCTG
  TTCGAAGAACTGGCCGCATTCCAAAGAAGCTTCTACAAAAAGTTTTTAATGATACCTGCCGCTCAGGTGG
  CCTAGGTGGTAGTCATGCCTTGCTTCTACTACGTAGTTGTGGTTCTCTCTTGCCTGAACTAAAGCTTGAA
  GAGAGAACAGAATTTGCTCATAGGATATGGGACACACTTCAGAAATTAGGTGCTGTGTATGATGTGAGTC
  ACTATAATGCTTTACTTAAAGTCTATCTTCAAAATGAATATAAATTCTCACCAACTGATTTCCTGGCAAA
  AATGGAGGAAGCAAACATTCAACCAAATCGAGTGACATACCAGAGATTGATTGCTTCTTATTGTAATGTA
  GGAGATATTGAAGGTGCCAGCAAGATTCTTGGATTTATGAAAACTAAGGATCTCCCAGTTACAGAGGCAG
  TATTCAGTGCCCTTGTGACAGGGCATGCCAGAGCTGGTGATATGGAGAATGCAGAAAACATTCTCACAGT
  GATGAGAGATGCCGGAATTGAGCCTGGTCCAGACACATACCTCGCATTATTGAATGCATATGCTGAGAAG
  GGCGACATTGACCATGTTAAGCAGACTCTGGAGAAGGTGGAGAAGTCCGAGCTTCACCTTATGGACCGTG
  ATTTACTGCAAATTATTTTTAGCTTCAGTAAAGCTGGGTATCCTCAGTATGTCTCAGAAATTTTGGAAAA
  AGTTACATGTGAAAGAAGATATATTCCAGATGCAATGAACCTCATTTTACTTTTAGTCACTGAAAAATTG
  GAAGATGTAGCGTTGCAAATTTTACTAGCATGCCCCGTATCAAAGGAAGATGGCCCAAGTGTCTTTGGCA
  GTTTCTTTTTACAACACTGTGTGACTATGAATACGCCTGTGGAGAAGCTAACAGACTACTGTAAGAAGTT
  AAAGGAAGTCCAGATGCACTCCTTTCCTCTGCAGTTCACCCTCCATTGTGCTTTACTCGCCAATAAAACT
  GATTTGGCAAAAGCCTTAATGAAGGCTGTGAAGGAGGAAGGTTTTCCTATCAGACCTCACTATTTCTGGC
  CATTGCTAGTTGGACGTCGGAAGGAAAAAAATGTTCAAGGTATAATTGAAATCCTCAAAGGAATGCAAGA
  ATTGGGAGTACATCCTGATCAGGAAACATATACAGATTATGTGATTCCATGCTTTGATAGTGTAAACTCA
  GCACGAGCCATTTTGCAGGAAAATGGATGTCTGTCTGATAGTGATATGTTTTCTCAAGCTGGATTGAGAA
  GTGAAGCAGCAAATGGGAACTTAGACTTTGTATTATCATTTTTGAAATCAAATACATTGCCCATCTCGCT
  GCAGTCTATAAGAAGTAGCCTACTGCTAGGCTTCAGGAGGTCTATGAATATAAATCTTTGGAGCGAGATA
  ACAGAATTGTTGTACAAGGATGGACGTTATTGCCAGGAGCCTCGAGGACCGACGGAAGCTGTTGGCTATT
  TTCTTTATAACTTGATTGACAGCATGAGTGACTCAGAAGTACAGGCCAAGGAGGAGCATTTGAGACAATA
  CTTCCATCAGCTGGAGAAGATGAATGTAAAAATTCCTGAAAATATCTACAGAGGCATTCGTAATCTCCTG
  GAAAGCTACCATGTTCCTGAATTGATTAAGGATGCTCACTTGTTGGTTGAGAGTAAGAATTTAGACTTTC
  AAAAAACTGTGCAACTTACATCATCTGAATTGGAGTCCACACTTGAAACACTAAAAGCTGAAAATCAACC
  TATAAGAGATGTCCTAAAGCAACTCATATTAGTGCTTTGTTCAGAAGAGAATATGCAAAAAGCCCTTGAA
  TTGAAAGCAAAATATGAATCCGACATGGTTACTGGTGGCTATGCAGCTTTAATAAATTTATGCTGTCGAC
  ATGATAAAGTAGAAGATGCCTTGAACTTGAAAGAAGAATTTGACCGCTTAGATTCATCTGCTGTCCTTGA
  CACCGGCAAGTATGTAGGCCTTGTAAGAGTATTGGCAAAGCATGGCAAGCTCCAAGATGCTATTAACATT
  CTGAAGGAGATGAAAGAGAAGGATGTTCTTATCAAAGATACAACAGCCTTGTCCTTTTTCCACATGCTAA
  ATGGCGCAGCTTTAAGAGGTGAAATTGAAACAGTAAAACAGTTGCATGAAGCCATCGTGACTCTAGGGTT
  AGCAGAACCATCCACCAACATAAGTTTCCCATTGGTCACTGTACACTTGGAAAAGGGCGACCTATCTACT
  GCTCTTGAGGTCGCCATTGACTGCTATGAAAAGTATAAAGTATTACCAAGGATTCATGATGTCTTGTGTA
  AACTGGTAGAGAAAGGCGAGACTGATCTAATTCAGAAAGCAATGGACTTTGTGAGCCAAGAACAAGGTGA
  AATGGTGATGCTCTATGATCTCTTCTTTGCCTTCCTACAAACAGGAAATTACAAAGAGGCCAAGAAGATC
  ATTGAGACTCCAGGGATTAGAGCTCGATCTGCAAGGCTTCAGTGGTTTTGTGACAGATGTGTTGCAAATA
  ATCAGGTTGAAACTCTGGAAAAATTAGTGGAGCTGACACAGAAGCTATTTGAATGTGATAGAGACCAGAT
  GTACTACAATCTGCTAAAACTGTATAAAATAAACGGTGACTGGCAAAGAGCTGATGCAGTCTGGAATAAA
  ATCCAAGAAGAAAATGTTATTCCTCGTGAAAAGACATTAAGATTATTAGCAGAAATCCTTAGAGAGGGTA
  ACCAGGAAGTTCCGTTTGACGTACCTGAGTTGTGGTATGAAGATGAAAAACATTCCCTGAATTCTTCGTC
  AGCCTCAACCACAGAACCTGATTTCCAGAAAGATATATTGATTGCCTGCCGATTGAACCAAAAAAAAGGG
  GCATATGATATTTTCCTGAATGCAAAAGAGCAAAACATTGTGTTTAATGCTGAAACCTACAGCAATCTCA
  TTAAATTACTGATGTCAGAAGATTATTTTACACAAGCAATGGAAGTGAAAGCATTCGCGGAGACCCACAT
  CAAGGGCTTCACACTGAACGATGCTGCCAACAGCCGCCTCATCATAACGCAAGTTAGGCGGGATTATTTG
  AAAGAGGCTGTGACAACACTGAAAACAGTATTGGATCAGCAGCAGACCCCTTCTAGGTTAGCAGTGACCC
  GTGTCATCCAGGCATTGGCCATGAAGGGTGATGTTGAAAACATAGAAGTAGTTCAGAAGATGTTAAATGG
  ACTCGAAGACTCCATTGGACTTTCAAAAATGGTTTTCATCAATAACATTGCTTTGGCTCAAATAAAGAAT
  AATAACATAGATGCCGCAATAGAAAACATTGAAAATATGCTTACTTCAGAGAATAAAGTCATTGAACCCC
  AATACTTCGGCTTGGCATACTTATTCAGAAAAGTAATAGAGGAGCAGTTGGAACCAGCAGTTGAAAAGAT
  AAGCATCATGGCGGAGAGATTGGCCAATCAGTTTGCAATTTATAAACCTGTCACTGATTTTTTCCTTCAA
  CTTGTGGATGCAGGCAAGGTGGATGATGCCAGAGCTCTCCTACAGAGATGTGGTGCAATTGCTGAACAAA
  CCCCGATTTTGTTGTTGTTCCTCCTTAGGAATTCTAGGAAACAAGGAAAGGCATCAACTGTGAAATCTGT
  GTTAGAATTGATTCCTGAATTAAATGAAAAGGAAGAAGCATACAATTCCCTCATGAAAAGCTATGTCTCA
  GAGAAAGATGTCACATCTGCTAAAGCACTGTATGAACATTTGACTGCAAAGAATACAAAATTGGATGATC
  TGTTTCTAAAGCGTTACGCATCTTTGCTGAAGTATGCTGGAGAGCCTGTCCCTTTCATTGAACCCCCTGA
  AAGCTTTGAATTTTATGCACAGCAGCTAAGAAAATTGAGGGAAAACTCTTCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216747 representing NM_133259
  Red=Cloning site Green=Tags(s)
  
  MAALLRSARWLLRAGAAPRLPLSLRLLPGGPGRLHAASYLPAARAGPVAGGLLSPARLYAIAAKEKDIQE
  ESTFSSRKISNQFDWALMRLDLSVRRTGRIPKKLLQKVFNDTCRSGGLGGSHALLLLRSCGSLLPELKLE
  ERTEFAHRIWDTLQKLGAVYDVSHYNALLKVYLQNEYKFSPTDFLAKMEEANIQPNRVTYQRLIASYCNV
  GDIEGASKILGFMKTKDLPVTEAVFSALVTGHARAGDMENAENILTVMRDAGIEPGPDTYLALLNAYAEK
  GDIDHVKQTLEKVEKSELHLMDRDLLQIIFSFSKAGYPQYVSEILEKVTCERRYIPDAMNLILLLVTEKL
  EDVALQILLACPVSKEDGPSVFGSFFLQHCVTMNTPVEKLTDYCKKLKEVQMHSFPLQFTLHCALLANKT
  DLAKALMKAVKEEGFPIRPHYFWPLLVGRRKEKNVQGIIEILKGMQELGVHPDQETYTDYVIPCFDSVNS
  ARAILQENGCLSDSDMFSQAGLRSEAANGNLDFVLSFLKSNTLPISLQSIRSSLLLGFRRSMNINLWSEI
  TELLYKDGRYCQEPRGPTEAVGYFLYNLIDSMSDSEVQAKEEHLRQYFHQLEKMNVKIPENIYRGIRNLL
  ESYHVPELIKDAHLLVESKNLDFQKTVQLTSSELESTLETLKAENQPIRDVLKQLILVLCSEENMQKALE
  LKAKYESDMVTGGYAALINLCCRHDKVEDALNLKEEFDRLDSSAVLDTGKYVGLVRVLAKHGKLQDAINI
  LKEMKEKDVLIKDTTALSFFHMLNGAALRGEIETVKQLHEAIVTLGLAEPSTNISFPLVTVHLEKGDLST
  ALEVAIDCYEKYKVLPRIHDVLCKLVEKGETDLIQKAMDFVSQEQGEMVMLYDLFFAFLQTGNYKEAKKI
  IETPGIRARSARLQWFCDRCVANNQVETLEKLVELTQKLFECDRDQMYYNLLKLYKINGDWQRADAVWNK
  IQEENVIPREKTLRLLAEILREGNQEVPFDVPELWYEDEKHSLNSSSASTTEPDFQKDILIACRLNQKKG
  AYDIFLNAKEQNIVFNAETYSNLIKLLMSEDYFTQAMEVKAFAETHIKGFTLNDAANSRLIITQVRRDYL
  KEAVTTLKTVLDQQQTPSRLAVTRVIQALAMKGDVENIEVVQKMLNGLEDSIGLSKMVFINNIALAQIKN
  NNIDAAIENIENMLTSENKVIEPQYFGLAYLFRKVIEEQLEPAVEKISIMAERLANQFAIYKPVTDFFLQ
  LVDAGKVDDARALLQRCGAIAEQTPILLLFLLRNSRKQGKASTVKSVLELIPELNEKEEAYNSLMKSYVS
  EKDVTSAKALYEHLTAKNTKLDDLFLKRYASLLKYAGEPVPFIEPPESFEFYAQQLRKLRENSS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133259
• ORF Size: 4182 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_133259.2, NP_573566.2
• RefSeq Size: 5095
• RefSeq ORF: 4185
• Locus ID: 10128
• Domains: PPR
• Gene Summary: This gene encodes a leucine-rich protein that has multiple pentatricopeptide repeats (PPR). The precise role of this protein is unknown but studies suggest it may play a role in cytoskeletal organization, vesicular transport, or in transcriptional regulation of both nuclear and mitochondrial genes. The protein localizes primarily to mitochondria and is predicted to have an N-terminal mitochondrial targeting sequence. Mutations in this gene are associated with the French-Canadian type of Leigh syndrome. [provided by RefSeq, Mar 2012]