Leucyl cystinyl aminopeptidase (LNPEP) (NM_175920) Human Tagged ORF Clone

CAT#: RG216948

LNPEP (GFP-tagged) - Human leucyl/cystinyl aminopeptidase (LNPEP), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LNPEP
• Synonyms: CAP; IRAP; P-LAP; PLAP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216948 representing NM_175920
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTGAAAACAGCATGTTTGAGGAAGAACCAGATGTGGTGGATTTAGCCAAAGAGCCTTGTTTACATC
  CTCTAGAGCCTGATGAGGTGGAATATGAGCCCCGGGGTTCCCGACTGCTGGTGCGGGGTCTTGGTGAGCA
  TGAGATGGAGGAGGATGAAGAGGATTATGAGTCATCAGCAAAGCTGCTGGGCATGTCCTTCATGAATAGA
  AGCTCAGGCCTTCGGAACAGTGCAACTGGTTACAGGCAGAGCCCAGATGGGGCTTGTTCAGTACCCTCTG
  CAAGGACCATGGTGGTCTGTGCTTTTGTCATCGTGGTTGCTGTTTCTGTAATCATGGTGATTTACTTACT
  GCCCAGATGTACCTTTACCAAAGAAGGCTGCCATAAAAAAAACCAGTCAATTGGACTAATTCAGCCATTT
  GCAACAAATGGGAAATTGTTTCCATGGGCACAGATCAGGCTTCCCACTGCCGTTGTGCCACTACGCTATG
  AACTCAGCCTACACCCGAACCTAACCTCGATGACATTCAGGGGTTCTGTGACAATTTCAGTTCAGGCTCT
  TCAGGTCACATGGAATATCATTCTTCATAGCACAGGTCATAATATTTCAAGAGTGACCTTTATGTCAGCA
  GTTTCAAGCCAAGAAAAACAAGCTGAGATCCTGGAATATGCATATCATGGACAGATCGCCATTGTTGCCC
  CCGAAGCCCTTCTAGCAGGGCACAATTATACGTTGAAGATAGAGTACTCGGCAAATATATCTAGTTCTTA
  TTATGGGTTTTATGGCTTCTCCTACACAGATGAAAGTAATGAGAAAAAGTACTTTGCAGCAACTCAGTTT
  GAACCCCTGGCAGCAAGATCTGCTTTTCCTTGTTTTGATGAACCAGCATTTAAAGCCACTTTTATCATCA
  AGATCATAAGGGATGAGCAATACACCGCTTTATCAAATATGCCTAAGAAGTCATCAGTCGTTCTAGATGA
  TGGACTTGTTCAGGATGAGTTTTCTGAGAGTGTGAAGATGAGCACTTACTTGGTTGCTTTCATTGTGGGA
  GAGATGAAGAACCTGAGTCAGGACGTAAATGGAACCCTGGTTTCTATATATGCTGTACCAGAAAAGATTG
  GTCAAGTTCATTATGCCTTGGAAACAACTGTGAAGCTTCTTGAGTTTTTTCAAAACTACTTTGAAATTCA
  GTACCCACTTAAGAAATTGGATTTGGTGGCTATTCCTGACTTTGAAGCAGGAGCAATGGAAAATTGGGGT
  TTGCTCACCTTCCGAGAGGAGACACTTCTGTATGACAGTAACACTTCTTCAATGGCGGATAGAAAGCTGG
  TGACTAAAATCATTGCTCATGAGCTGGCCCACCAGTGGTTTGGCAATCTGGTAACAATGAAGTGGTGGAA
  TGACCTATGGCTAAATGAAGGTTTTGCCACTTTCATGGAGTATTTCTCTTTGGAAAAAATATTCAAAGAG
  CTTTCTAGTTATGAAGATTTCTTAGATGCTCGATTTAAAACCATGAAGAAAGATTCCTTAAATTCATCTC
  ATCCAATATCATCATCTGTTCAGTCTTCAGAACAAATTGAAGAAATGTTTGATTCTCTTTCCTATTTTAA
  GGGATCTTCTCTCTTGTTGATGTTGAAAACTTACCTTAGTGAAGATGTGTTTCAACATGCTGTTGTCCTT
  TACCTGCATAATCACAGCTATGCATCTATTCAAAGTGATGATCTGTGGGATAGTTTTAATGAGGTCACAA
  ACCAAACACTAGATGTAAAGAGAATGATGAAAACCTGGACCCTGCAGAAAGGATTTCCTTTAGTGACTGT
  TCAAAAGAAAGGAAAGGAACTTTTTATACAACAAGAGAGATTCTTTTTAAATATGAAGCCTGAAATTCAG
  CCTTCAGATACAAGCTACCTGTGGCATATTCCACTATCCTATGTCACTGAAGGAAGAAATTATTCAAAAT
  ATCAATCGGTATCATTACTGGATAAGAAATCAGGTGTCATCAATCTTACAGAAGAAGTGCTGTGGGTCAA
  AGTGAATATAAACATGAATGGTTATTATATTGTACACTATGCAGATGATGATTGGGAAGCACTAATCCAT
  CAGTTGAAAATAAATCCTTATGTTCTGAGTGACAAAGACCGAGCCAACCTTATCAACAACATCTTTGAAC
  TTGCAGGCCTAGGCAAGGTACCTCTCAAGAGGGCCTTTGATTTGATTAATTATCTTGGAAATGAGAACCA
  TACTGCACCCATCACCGAAGCCCTGTTTCAGACAGACCTCATCTATAACCTCCTTGAAAAACTGGGATAC
  ATGGATCTGGCCTCAAGACTGGTGACTAGGGTATTTAAATTACTTCAAAACCAAATTCAACAACAAACTT
  GGACTGATGAGGGCACTCCATCTATGCGAGAGCTTCGGTCAGCCCTGCTAGAGTTTGCTTGCACCCACAA
  CCTGGGGAACTGCTCTACTACTGCCATGAAACTGTTTGATGACTGGATGGCATCCAATGGAACTCAAAGC
  CTACCTACTGATGTCATGACAACTGTGTTCAAAGTTGGAGCAAAAACTGACAAAGGCTGGTCATTCCTTT
  TGGGCAAATACATTTCTATAGGCTCTGAAGCAGAGAAGAACAAAATACTAGAAGCACTTGCCAGCTCAGA
  GGATGTGCGGAAGCTTTACTGGTTAATGAAAAGTAGCCTGAATGGAGATAACTTCCGAACACAGAAGCTG
  TCTTTTATCATTAGAACAGTGGGTCGACATTTTCCTGGACACTTACTGGCATGGGATTTTGTCAAAGAGA
  ACTGGAATAAGCTTGTACAGAAGTTCCCTCTGGGGTCCTATACCATACAAAATATTGTTGCTGGATCAAC
  TTACCTGTTTTCAACAAAGACACATTTATCTGAGGTTCAGGCATTCTTTGAAAATCAGTCAGAGGCAACC
  TTCCGGCTTCGTTGTGTCCAGGAGGCTTTGGAAGTCATTCAGTTGAATATCCAGTGGATGGAGAAGAACC
  TCAAAAGTCTCACATGGTGGCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216948 representing NM_175920
  Red=Cloning site Green=Tags(s)
  
  MIENSMFEEEPDVVDLAKEPCLHPLEPDEVEYEPRGSRLLVRGLGEHEMEEDEEDYESSAKLLGMSFMNR
  SSGLRNSATGYRQSPDGACSVPSARTMVVCAFVIVVAVSVIMVIYLLPRCTFTKEGCHKKNQSIGLIQPF
  ATNGKLFPWAQIRLPTAVVPLRYELSLHPNLTSMTFRGSVTISVQALQVTWNIILHSTGHNISRVTFMSA
  VSSQEKQAEILEYAYHGQIAIVAPEALLAGHNYTLKIEYSANISSSYYGFYGFSYTDESNEKKYFAATQF
  EPLAARSAFPCFDEPAFKATFIIKIIRDEQYTALSNMPKKSSVVLDDGLVQDEFSESVKMSTYLVAFIVG
  EMKNLSQDVNGTLVSIYAVPEKIGQVHYALETTVKLLEFFQNYFEIQYPLKKLDLVAIPDFEAGAMENWG
  LLTFREETLLYDSNTSSMADRKLVTKIIAHELAHQWFGNLVTMKWWNDLWLNEGFATFMEYFSLEKIFKE
  LSSYEDFLDARFKTMKKDSLNSSHPISSSVQSSEQIEEMFDSLSYFKGSSLLLMLKTYLSEDVFQHAVVL
  YLHNHSYASIQSDDLWDSFNEVTNQTLDVKRMMKTWTLQKGFPLVTVQKKGKELFIQQERFFLNMKPEIQ
  PSDTSYLWHIPLSYVTEGRNYSKYQSVSLLDKKSGVINLTEEVLWVKVNINMNGYYIVHYADDDWEALIH
  QLKINPYVLSDKDRANLINNIFELAGLGKVPLKRAFDLINYLGNENHTAPITEALFQTDLIYNLLEKLGY
  MDLASRLVTRVFKLLQNQIQQQTWTDEGTPSMRELRSALLEFACTHNLGNCSTTAMKLFDDWMASNGTQS
  LPTDVMTTVFKVGAKTDKGWSFLLGKYISIGSEAEKNKILEALASSEDVRKLYWLMKSSLNGDNFRTQKL
  SFIIRTVGRHFPGHLLAWDFVKENWNKLVQKFPLGSYTIQNIVAGSTYLFSTKTHLSEVQAFFENQSEAT
  FRLRCVQEALEVIQLNIQWMEKNLKSLTWWL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175920
• ORF Size: 3033 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175920.3, NP_787116.2
• RefSeq Size: 4262 bp
• RefSeq ORF: 3036 bp
• Locus ID: 4012
• Cytogenetics: 5q15
• Protein Families: Druggable Genome, Protease, Secreted Protein, Transmembrane
• Protein Pathways: Renin-angiotensin system
• Gene Summary: 'This gene encodes a zinc-dependent aminopeptidase that cleaves vasopressin, oxytocin, lys-bradykinin, met-enkephalin, dynorphin A and other peptide hormones. The protein can be secreted in maternal serum, reside in intracellular vesicles with the insulin-responsive glucose transporter GLUT4, or form a type II integral membrane glycoprotein. The protein catalyzes the final step in the conversion of angiotensinogen to angiotensin IV (AT4) and is also a receptor for AT4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]'