BRD8 (NM_006696) Human Tagged ORF Clone

CAT#: RG217051

BRD8 (GFP-tagged) - Human bromodomain containing 8 (BRD8), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: BRD8
• Synonyms: p120; SMAP; SMAP2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217051 representing NM_006696
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACGGGAACGGGCAAACACAAGCTGCTAAGCACTGGCCCCACAGAGCCATGGTCCATCCGAGAGA
  AGCTATGTTTAGCATCTTCTGTCATGAGAAGTGGCGATCAAAATTGGGTATCAGTTAGCAGAGCAATCAA
  GCCCTTTGCAGAACCTGGCCGCCCTCCAGACTGGTTCTCTCAAAAACATTGTGCTTCCCAGTACTCGGAG
  CTTTTAGAGACCACTGAGACACCAAAACGGAAACGAGGTGAAAAGGGAGAAGTGGTGGAAACTGTTGAAG
  ATGTTATTGTTCGGAAATTGACTGCTGAGCGAGTTGAAGAACTAAAGAAAGTGATAAAGGAAACCCAGGA
  GAGATATAGACGGCTAAAGAGAGATGCAGAACTAATTCAAGCTGGACACATGGACAGCAGACTGGATGAG
  CTTTGCAATGACATTGCAACGAAAAAGAAATTGGAAGAAGAGGAGGCTGAAGTAAAGAGGAAGGCTACAG
  ATGCTGCATACCAGGCTCGTCAAGCAGTAAAAACACCCCCCCGGAGGTTACCCACTGTGATGGTTCGCTC
  TCCTATAGATTCTGCCTCCCCAGGAGGTGATTATCCACTTGGGGACTTGACTCCAACCACTATGGAAGAG
  GCTACCTCTGGGGTAACCCCCGGGACTTTGCCGAGTACCCCAGTCACCTCGTTTCCTGGGATTCCTGACA
  CCCTTCCTCCAGGCTCTGCACCCTTAGAAGCCCCCATGACCCCAGTAACAGATGATTCACCCCAGAAAAA
  GATGCTTGGACAGAAAGCAACTCCACCCCCCTCCCCTCTGCTGTCAGAGCTCTTGAAGAAGGGCAGCCTC
  CTGCCTACTAGCCCCAGACTGGTCAATGAGAGTGAAATGGCTGTGGCTTCTGGCCACCTGAACAGTACAG
  GTGTCCTCCTGGAGGTAGGCGGGGTCCTTCCCATGATACATGGTGGGGAGATACAGCAAACACCCAATAC
  TGTTGCAGCCTCCCCTGCTGCATCAGGTGCTCCCACTCTTTCCCGGCTTTTAGAAGCTGGTCCTACACAG
  TTCACCACACCTCTTGCTTCCTTCACTACTGTTGCCAGTGAGCCTCCAGTTAAACTTGTGCCACCCCCTG
  TAGAGTCTGTGTCCCAAGCTACCATTGTCATGATGCCTGCGCTGCCAGCACCATCCTCTGCTCCGGCTGT
  CTCCACTACTGAAAGTGTAGCTCCAGTGAGTCAACCCGACAACTGTGTTCCCATGGAGGCTGTGGGGGAT
  CCACATACTGTGACTGTTTCCATGGACAGCAGTGAAATATCCATGATCATCAATTCTATCAAAGAAGAGT
  GTTTTCGATCAGGGGTAGCAGAGGCTCCTGTTGGATCAAAGGCTCCCAGCATAGATGGGAAGGAAGAATT
  AGATCTGGCTGAGAAGATGGATATTGCTGTGTCTTACACAGGTGAAGAGCTGGATTTTGAGACTGTTGGA
  GACATCATTGCCATCATTGAGGACAAGGTAGATGATCATCCTGAAGTGCTGGATGTGGCAGCAGTGGAAG
  CAGCACTGTCATTTTGTGAAGAAAATGATGATCCTCAGTCCCTGCCTGGCCCCTGGGAGCATCCTATCCA
  GCAGGAGCGGGACAAGCCAGTACCTCTCCCTGCACCAGAAATGACGGTCAAGCAAGAGAGACTGGACTTT
  GAGGAAACGGAAAACAAGGGAATACATGAACTGGTGGACATCAGGGAGCCCAGTGCAGAGATCAAGGTGG
  AACCTGCAGAACCAGAGCCAGTCATTTCAGGAGCCGAAATAGTAGCTGGAGTTGTTCCAGCCACAAGTAT
  GGAGCCACCAGAACTCAGGAGTCAGGACTTAGATGAGGAACTGGGAAGTACTGCAGCTGGAGAGATTGTT
  GAAGCAGATGTTGCCATTGGGAAAGGCGATGAGACTCCACTTACAAATGTGAAGACAGAGGCATCCCCTG
  AAAGCATGTTGTCTCCATCACATGGCTCAAATCCCATTGAAGATCCTTTAGAGGCAGAGACTCAGCACAA
  GTTTGAAATGTCAGACTCATTGAAAGAAGAATCAGGGACTATTTTTGGAAGCCAGATAAAGGATGCCCCA
  GGTGAGGATGAGGAGGAAGATGGTGTCAGTGAAGCGGCCAGCCTAGAGGAGCCTAAGGAAGAGGATCAAG
  GAGAAGGCTACTTGTCAGAAATGGATAATGAACCTCCTGTGAGCGAGAGTGATGATGGCTTCAGCATACA
  CAATGCTACACTGCAGTCACACACACTGGCAGACTCCATCCCCAGCAGCCCTGCTTCTTCACAGTTCTCT
  GTCTGTAGTGAGGATCAGGAAGCTATTCAGGCACAGAAAATTTGGAAGAAAGCCATCATGCTTGTATGGA
  GAGCTGCAGCTAATCATAGGTATGCCAATGTCTTCCTGCAGCCTGTTACAGATGACATAGCACCTGGCTA
  CCACAGCATTGTGCAGAGGCCTATGGATTTGTCAACTATTAAGAAAAACATAGAAAATGGACTGATCCGA
  AGCACAGCTGAATTTCAGCGTGACATTATGCTGATGTTTCAGAATGCTGTAATGTACAATAGCTCAGACC
  ATGATGTCTATCACATGGCAGTGGAGATGCAGCGAGATGTCTTGGAACAGATCCAGCAATTCTTGGCCAC
  GCAGTTGATTATGCAAACATCCGAGTCTGGGATCAGTGCTAAAAGTCTTCGAGGGAGAGATTCTACCCGC
  AAACAGGATGCTTCAGAGAAGGACAGTGTCCCAATGGGCTCTCCTGCCTTCCTTCTCTCTCTCTTTGATG
  GAGGAACCAGGGGACGCCGCTGTGCCATTGAAGCAGATATGAAGATGAAAAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217051 representing NM_006696
  Red=Cloning site Green=Tags(s)
  
  MATGTGKHKLLSTGPTEPWSIREKLCLASSVMRSGDQNWVSVSRAIKPFAEPGRPPDWFSQKHCASQYSE
  LLETTETPKRKRGEKGEVVETVEDVIVRKLTAERVEELKKVIKETQERYRRLKRDAELIQAGHMDSRLDE
  LCNDIATKKKLEEEEAEVKRKATDAAYQARQAVKTPPRRLPTVMVRSPIDSASPGGDYPLGDLTPTTMEE
  ATSGVTPGTLPSTPVTSFPGIPDTLPPGSAPLEAPMTPVTDDSPQKKMLGQKATPPPSPLLSELLKKGSL
  LPTSPRLVNESEMAVASGHLNSTGVLLEVGGVLPMIHGGEIQQTPNTVAASPAASGAPTLSRLLEAGPTQ
  FTTPLASFTTVASEPPVKLVPPPVESVSQATIVMMPALPAPSSAPAVSTTESVAPVSQPDNCVPMEAVGD
  PHTVTVSMDSSEISMIINSIKEECFRSGVAEAPVGSKAPSIDGKEELDLAEKMDIAVSYTGEELDFETVG
  DIIAIIEDKVDDHPEVLDVAAVEAALSFCEENDDPQSLPGPWEHPIQQERDKPVPLPAPEMTVKQERLDF
  EETENKGIHELVDIREPSAEIKVEPAEPEPVISGAEIVAGVVPATSMEPPELRSQDLDEELGSTAAGEIV
  EADVAIGKGDETPLTNVKTEASPESMLSPSHGSNPIEDPLEAETQHKFEMSDSLKEESGTIFGSQIKDAP
  GEDEEEDGVSEAASLEEPKEEDQGEGYLSEMDNEPPVSESDDGFSIHNATLQSHTLADSIPSSPASSQFS
  VCSEDQEAIQAQKIWKKAIMLVWRAAANHRYANVFLQPVTDDIAPGYHSIVQRPMDLSTIKKNIENGLIR
  STAEFQRDIMLMFQNAVMYNSSDHDVYHMAVEMQRDVLEQIQQFLATQLIMQTSESGISAKSLRGRDSTR
  KQDASEKDSVPMGSPAFLLSLFDGGTRGRRCAIEADMKMKK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006696
• ORF Size: 2853 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_006696.3, NP_006687.3
• RefSeq Size: 3253
• RefSeq ORF: 2856
• Locus ID: 10902
• Domains: BROMO
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: The protein encoded by this gene interacts with thyroid hormone receptor in a ligand-dependent manner and enhances thyroid hormone-dependent activation from thyroid response elements. This protein contains a bromodomain and is thought to be a nuclear receptor coactivator. Multiple alternatively spliced transcript variants that encode distinct isoforms have been identified. [provided by RefSeq, Jul 2014]