LLGL1 (NM_004140) Human Tagged ORF Clone

CAT#: RG217078

LLGL1 (GFP-tagged) - Human lethal giant larvae homolog 1 (Drosophila) (LLGL1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LLGL1
• Synonyms: DLG4; HUGL; HUGL-1; HUGL1; Lgl1; LLGL; Mgl1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217078 representing NM_004140
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGAAGTTTCGGTTCCGGCGGCAGGGCGCCGACCCGCAGCGCGAGAAGCTCAAGCAGGAGCTTTTCG
  CCTTCAACAAGACTGTGGAGCATGGCTTCCCCAATCAGCCCAGCGCCCTGGCCTTCGACCCGGAACTTCG
  CATCATGGCCATCGGCACCAGGTCTGGGGCTGTCAAGATCTATGGTGCACCTGGCGTGGAGTTCACAGGC
  CTGCACCGGGATGCAGCCACTGTCACACAGATGCACTTCTTGACCGGCCAGGGCCGCCTCCTGTCCCTGC
  TTGATGACAGCAGTCTGCATCTCTGGGAGATTGTCCACCATAATGGCTGTGCCCACCTGGAAGAAGCACT
  CAGTTTCCAGCTGCCCAGCCGGCCCGGCTTTGATGGTGCCAGTGCTCCGCTCAGCCTTACCCGAGTCACA
  GTGGTCCTGCTGGTGGCTGCCGGCGACATAGCAGCCCTGGGCACTGAGGGCAGCAGTGTCTTCTTCCTGG
  ATGTTACCACCCTGACCCTGCTCGAGGGGCAGACGCTTGCCCCAGGCGAGGTTCTGCGCAGCGTGCCAGA
  CGACTACCGCTGTGGGAAGGCACTGGGCCCCGTGGAGTCACTCCAGGGACACCTGCGGGACCCCACAAAG
  ATTCTCATTGGCTACAGCCGGGGCCTGCTGGTCATCTGGAACCAGGCCTCGCAGTGTGTGGACCACATCT
  TCCTGGGGAACCAGCAGCTGGAGAGCCTATGCTGGGGGCGTGATAGCAGCACTGTGGTCAGCTCACACAG
  CGATGGCAGCTATGCTGTCTGGTCTGTGGATGCCGGCAGCTTCCCAACGCTGCAGCCCACGGTAGCCACC
  ACACCTTACGGCCCCTTTCCCTGCAAGGCCATTAACAAGATTCTGTGGCGGAACTGTGAATCTGGGGGCC
  ACTTTATCATCTTCAGCGGTGGCATGCCCCGTGCCAGCTATGGTGACCGCCACTGTGTAAGTGTGCTTCG
  AGCCGAGACATTGGTGACGCTGGACTTCACTTCCCGCATCATCGACTTCTTCACAGTGCACAGCACACGG
  CCCGAGGATGAATTTGATGACCCCCAGGCCCTGGCTGTGCTGCTGGAAGAGGAGCTGGTGGTGCTGGACC
  TGCAGACTCCTGGCTGGCCAGCTGTGCCTGCCCCATACCTGGCCCCGCTGCACTCCTCTGCAATCACTTG
  CTCGGCCCACGTGGCCAGTGTCCCCGCCAAGCTGTGGGCCCGCATTGTGAGCGCTGGCGAGCAGCAGAGC
  CCCCAGCCTGTCTCCAGTGCCTTGAGCTGGCCCATCACTGGGGGCCGAAACCTGGCCCAGGAGCCGTCAC
  AGCGAGGGCTGCTGCTGACGGGCCATGAGGACGGCACCGTGAGGTTCTGGGATGCCTCGGGTGTGGCGCT
  GCGGCCGCTCTATAAGCTGAGCACAGCTGGCCTCTTCCAGACAGACTGTGAGCACGCTGACAGCCTGGCC
  CAGGCTGCCGAGGACGACTGGCCACCCTTCCGCAAGGTGGGCTGCTTCGATCCCTACAGTGACGATCCCC
  GGCTTGGCGTGCAGAAGGTTGCTCTCTGCAAGTATACAGCCCAGATGGTGGTGGCTGGCACTGCAGGCCA
  GGTGCTGGTACTGGAGCTTAGTGATGTGCCGGTGGAGCAGGCGGTCAGCGTGGCCATCATAGACCTCCTC
  CAGGACCGCGAGGGCTTCACATGGAAGGGCCACGAGCGGCTGAGCCCACGCACGGGGCCGCTGCCCTGGC
  CTGCTGGCTTCCAGCCCCGTGTCCTGGTGCAGTGCCTGCCGCCAGCTGCTGTAACCGCTGTCACACTCCA
  CACCGAGTGGAGCCTCGTGGCTTTTGGCACCAGTCATGGCTTTGGCCTCTTCGACTACCAGCGCAAGAGC
  CCTGTGCTGGCCAGGTGCACTCTTCACCCCAATGACTCCCTGGCCATGGAGGGTCCGCTCTCCCGGGTGA
  AGTCTCTCAAGAAGTCACTGCGCCAGTCTTTCCGGCGCATTCGCAAGAGTCGTGTCTCTGGCAAGAAGCG
  GGCTGCTAATGCCAGCAGCAAGTTGCAGGAAGCCAATGCACAGCTGGCTGAGCAGGCCTGCCCCCACGAC
  GTGGAGATGACGCCCGTGCAGCGCCGCATTGAGCCCCGCTCTGCCGATGACTCCTTGTCGGGTGTCGTGC
  GTTGCCTATACTTTGCCGACACATTCCTTCGAGATGGGGCCCACCACGGGCCCACCATGTGGGCTGGCAC
  CAACTCAGGCTCTGTGTTCGCCTATGCACTGGAGGTGCCGGCAGCAGCAGTGGGTGGTGAGAAGCGGCCT
  GAGCAAGCGGTGGAGGCCGTGCTGGGCAAGGAGGTGCAGCTGATGCACCGGGCGCCTGTGGTGGCCATTG
  CCGTGTTGGACGGGCGTGGCCGCCCACTGCCCGAGCCCTACGAGGCCTCACGGGACCTGGCGCAGGCACC
  TGACATGCAGGGTGGTCACGCTGTGCTCATCGCATCTGAGGAGCAGTTCAAGGTGTTCACACTGCCCAAG
  GTGAGCGCGAAGACCAAGTTCAAGCTGACGGCCCATGAGGGCTGTCGTGTGCGCAAGGTGGCACTGGCCA
  CGTTTGCCAGTGTGGCCTGCGAGGACTATGCTGAGACCTGCCTGGCCTGCCTCACCAACCTGGGTGACGT
  CCACGTCTTCTCGGTGCCTGGCCTGCGGCCCCAGGTGCACTATTCCTGCATCCGGAAGGAGGACATCAGC
  GGCATCGCTTCGTGCGTCTTTACGCGCCATGGCCAGGGCTTTTACCTGATATCCCCATCAGAATTTGAAC
  GCTTCTCCCTAAGTGCCCGGAACATCACAGAGCCGCTCTGCTCTCTGGACATTAACTGGCCCCGCGATGC
  CACCCAGGCCAGTTACAGGATCCGAGAGTCACCCAAGCTGAGCCAGGCTAACGGGACCCCAAGCATCCTG
  CTGGCCCCACAGAGCCTTGATGGAAGCCCTGATCCAGCCCACAGCATGGGACCTGACACCCCGGAGCCAC
  CCGAGGCTGCACTCTCACCCATGTCCATCGACTCAGCCACCAGTGCTGACACCACGCTGGACACGACAGG
  GGACGTCACAGTGGAAGATGTGAAGGATTTCCTGGGCTCCTCTGAGGAGTCAGAGAAGAACCTGAGGAAC
  CTGGCAGAAGACGAGGCCCACGCCTGTGCCATCCTGATCAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217078 representing NM_004140
  Red=Cloning site Green=Tags(s)
  
  MMKFRFRRQGADPQREKLKQELFAFNKTVEHGFPNQPSALAFDPELRIMAIGTRSGAVKIYGAPGVEFTG
  LHRDAATVTQMHFLTGQGRLLSLLDDSSLHLWEIVHHNGCAHLEEALSFQLPSRPGFDGASAPLSLTRVT
  VVLLVAAGDIAALGTEGSSVFFLDVTTLTLLEGQTLAPGEVLRSVPDDYRCGKALGPVESLQGHLRDPTK
  ILIGYSRGLLVIWNQASQCVDHIFLGNQQLESLCWGRDSSTVVSSHSDGSYAVWSVDAGSFPTLQPTVAT
  TPYGPFPCKAINKILWRNCESGGHFIIFSGGMPRASYGDRHCVSVLRAETLVTLDFTSRIIDFFTVHSTR
  PEDEFDDPQALAVLLEEELVVLDLQTPGWPAVPAPYLAPLHSSAITCSAHVASVPAKLWARIVSAGEQQS
  PQPVSSALSWPITGGRNLAQEPSQRGLLLTGHEDGTVRFWDASGVALRPLYKLSTAGLFQTDCEHADSLA
  QAAEDDWPPFRKVGCFDPYSDDPRLGVQKVALCKYTAQMVVAGTAGQVLVLELSDVPVEQAVSVAIIDLL
  QDREGFTWKGHERLSPRTGPLPWPAGFQPRVLVQCLPPAAVTAVTLHTEWSLVAFGTSHGFGLFDYQRKS
  PVLARCTLHPNDSLAMEGPLSRVKSLKKSLRQSFRRIRKSRVSGKKRAANASSKLQEANAQLAEQACPHD
  VEMTPVQRRIEPRSADDSLSGVVRCLYFADTFLRDGAHHGPTMWAGTNSGSVFAYALEVPAAAVGGEKRP
  EQAVEAVLGKEVQLMHRAPVVAIAVLDGRGRPLPEPYEASRDLAQAPDMQGGHAVLIASEEQFKVFTLPK
  VSAKTKFKLTAHEGCRVRKVALATFASVACEDYAETCLACLTNLGDVHVFSVPGLRPQVHYSCIRKEDIS
  GIASCVFTRHGQGFYLISPSEFERFSLSARNITEPLCSLDINWPRDATQASYRIRESPKLSQANGTPSIL
  LAPQSLDGSPDPAHSMGPDTPEPPEAALSPMSIDSATSADTTLDTTGDVTVEDVKDFLGSSEESEKNLRN
  LAEDEAHACAILIK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004140
• ORF Size: 3192 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004140.3, NP_004131.3
• RefSeq Size: 4225 bp
• RefSeq ORF: 3195 bp
• Locus ID: 3996
• Cytogenetics: 17p11.2
• Protein Families: Druggable Genome
• Protein Pathways: Tight junction
• Gene Summary: 'This gene encodes a protein that is similar to a tumor suppressor in Drosophila. The protein is part of a cytoskeletal network and is associated with nonmuscle myosin II heavy chain and a kinase that specifically phosphorylates this protein at serine residues. The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]'