LLGL1 (NM_004140) Human Untagged Clone

CAT#: SC303440

LLGL1 (untagged)-Human lethal giant larvae homolog 1 (Drosophila) (LLGL1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LLGL1
• Synonyms: DLG4; HUGL; HUGL-1; HUGL1; Lgl1; LLGL; Mgl1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004140, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATGAAGTTTCGGTTCCGGCGGCAGGGCGCCGACCCGCAGCGCGAGAAGCTCAAGCAGGAGCTTTTCG
  CCTTCAACAAGACTGTGGAGCATGGCTTCCCCAATCAGCCCAGCGCCCTGGCCTTCGACCCGGAACTTCG
  CATCATGGCCATCGGCACCAGGTCTGGGGCTGTCAAGATCTATGGTGCACCTGGCGTGGAGTTCACAGGC
  CTGCACCGGGATGCAGCCACTGTCACACAGATGCACTTCTTGACCGGCCAGGGCCGCCTCCTGTCCCTGC
  TTGATGACAGCAGTCTGCATCTCTGGGAGATTGTCCACCATAATGGCTGTGCCCACCTGGAAGAAGCACT
  CAGTTTCCAGCTGCCCAGCCGGCCCGGCTTTGATGGTGCCAGTGCTCCGCTCAGCCTTACCCGAGTCACA
  GTGGTCCTGCTGGTGGCTGCCGGCGACATAGCAGCCCTGGGCACTGAGGGCAGCAGTGTCTTCTTCCTGG
  ATGTTACCACCCTGACCCTGCTCGAGGGGCAGACGCTTGCCCCAGGCGAGGTTCTGCGCAGCGTGCCAGA
  CGACTACCGCTGTGGGAAGGCACTGGGCCCCGTGGAGTCACTCCAGGGACACCTGCGGGACCCCACAAAG
  ATTCTCATTGGCTACAGCCGGGGCCTGCTGGTCATCTGGAACCAGGCCTCGCAGTGTGTGGACCACATCT
  TCCTGGGGAACCAGCAGCTGGAGAGCCTATGCTGGGGGCGTGATAGCAGCACTGTGGTCAGCTCACACAG
  CGATGGCAGCTATGCTGTCTGGTCTGTGGATGCCGGCAGCTTCCCAACGCTGCAGCCCACGGTAGCCACC
  ACACCTTACGGCCCCTTTCCCTGCAAGGCCATTAACAAGATTCTGTGGCGGAACTGTGAATCTGGGGGCC
  ACTTTATCATCTTCAGCGGTGGCATGCCCCGTGCCAGCTATGGTGACCGCCACTGTGTAAGTGTGCTTCG
  AGCCGAGACATTGGTGACGCTGGACTTCACTTCCCGCATCATCGACTTCTTCACAGTGCACAGCACACGG
  CCCGAGGATGAATTTGATGACCCCCAGGCCCTGGCTGTGCTGCTGGAAGAGGAGCTGGTGGTGCTGGACC
  TGCAGACTCCTGGCTGGCCAGCTGTGCCTGCCCCATACCTGGCCCCGCTGCACTCCTCTGCAATCACTTG
  CTCGGCCCACGTGGCCAGTGTCCCCGCCAAGCTGTGGGCCCGCATTGTGAGCGCTGGCGAGCAGCAGAGC
  CCCCAGCCTGTCTCCAGTGCCTTGAGCTGGCCCATCACTGGGGGCCGAAACCTGGCCCAGGAGCCGTCAC
  AGCGAGGGCTGCTGCTGACGGGCCATGAGGACGGCACCGTGAGGTTCTGGGATGCCTCGGGTGTGGCGCT
  GCGGCCGCTCTATAAGCTGAGCACAGCTGGCCTCTTCCAGACAGACTGTGAGCACGCTGACAGCCTGGCC
  CAGGCTGCCGAGGACGACTGGCCACCCTTCCGCAAGGTGGGCTGCTTCGATCCCTACAGTGACGATCCCC
  GGCTTGGCGTGCAGAAGGTTGCTCTCTGCAAGTATACAGCCCAGATGGTGGTGGCTGGCACTGCAGGCCA
  GGTGCTGGTACTGGAGCTTAGTGATGTGCCGGTGGAGCAGGCGGTCAGCGTGGCCATCATAGACCTCCTC
  CAGGACCGCGAGGGCTTCACATGGAAGGGCCACGAGCGGCTGAGCCCACGCACGGGGCCGCTGCCCTGGC
  CTGCTGGCTTCCAGCCCCGTGTCCTGGTGCAGTGCCTGCCGCCAGCTGCTGTAACCGCTGTCACACTCCA
  CACCGAGTGGAGCCTCGTGGCTTTTGGCACCAGTCATGGCTTTGGCCTCTTCGACTACCAGCGCAAGAGC
  CCTGTGCTGGCCAGGTGCACTCTTCACCCCAATGACTCCCTGGCCATGGAGGGTCCGCTCTCCCGGGTGA
  AGTCTCTCAAGAAGTCACTGCGCCAGTCTTTCCGGCGCATTCGCAAGAGTCGTGTCTCTGGCAAGAAGCG
  GGCTGCTAATGCCAGCAGCAAGTTGCAGGAAGCCAATGCACAGCTGGCTGAGCAGGCCTGCCCCCACGAC
  GTGGAGATGACGCCCGTGCAGCGCCGCATTGAGCCCCGCTCTGCCGATGACTCCTTGTCGGGTGTCGTGC
  GTTGCCTATACTTTGCCGACACATTCCTTCGAGATGGGGCCCACCACGGGCCCACCATGTGGGCTGGCAC
  CAACTCAGGCTCTGTGTTCGCCTATGCACTGGAGGTGCCGGCAGCAGCAGTGGGTGGTGAGAAGCGGCCT
  GAGCAAGCGGTGGAGGCCGTGCTGGGCAAGGAGGTGCAGCTGATGCACCGGGCGCCTGTGGTGGCCATTG
  CCGTGTTGGACGGGCGTGGCCGCCCACTGCCCGAGCCCTACGAGGCCTCACGGGACCTGGCGCAGGCACC
  TGACATGCAGGGTGGTCACGCTGTGCTCATCGCATCTGAGGAGCAGTTCAAGGTGTTCACACTGCCCAAG
  GTGAGCGCGAAGACCAAGTTCAAGCTGACGGCCCATGAGGGCTGTCGTGTGCGCAAGGTGGCACTGGCCA
  CGTTTGCCAGTGTGGCCTGCGAGGACTATGCTGAGACCTGCCTGGCCTGCCTCACCAACCTGGGTGACGT
  CCACGTCTTCTCGGTGCCTGGCCTGCGGCCCCAGGTGCACTATTCCTGCATCCGGAAGGAGGACATCAGC
  GGCATCGCTTCGTGCGTCTTTACGCGCCATGGCCAGGGCTTTTACCTGATATCCCCATCAGAATTTGAAC
  GCTTCTCCCTAAGTGCCCGGAACATCACAGAGCCGCTCTGCTCTCTGGACATTAACTGGCCCCGCGATGC
  CACCCAGGCCAGTTACAGGATCCGAGAGTCACCCAAGCTGAGCCAGGCTAACGGGACCCCAAGCATCCTG
  CTGGCCCCACAGAGCCTTGATGGAAGCCCTGATCCAGCCCACAGCATGGGACCTGACACCCCGGAGCCAC
  CCGAGGCTGCACTCTCACCCATGTCCATCGACTCAGCCACCAGTGCTGACACCACGCTGGACACGACAGG
  GGACGTCACAGTGGAAGATGTGAAGGATTTCCTGGGCTCCTCTGAGGAGTCAGAGAAGAACCTGAGGAAC
  CTGGCAGAAGACGAGGCCCACGCCTGTGCCATCCTGATCAAATGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_004140
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004140.3, NP_004131.3
• RefSeq Size: 4225 bp
• RefSeq ORF: 3195 bp
• Locus ID: 3996
• Cytogenetics: 17p11.2
• Protein Families: Druggable Genome
• Protein Pathways: Tight junction
• Gene Summary: 'This gene encodes a protein that is similar to a tumor suppressor in Drosophila. The protein is part of a cytoskeletal network and is associated with nonmuscle myosin II heavy chain and a kinase that specifically phosphorylates this protein at serine residues. The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]'