JLP (SPAG9) (NM_003971) Human Tagged ORF Clone

CAT#: RG217252

SPAG9 (GFP-tagged) - Human sperm associated antigen 9 (SPAG9), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SPAG9
• Synonyms: CT89; HLC-6; HLC4; HLC6; JIP-4; JIP4; JLP; PHET; PIG6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217252 representing NM_003971
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGGAGGACGGTGTGGTGTATCAGGAGGAGCCCGGCGGCTCCGGGGCCGTGATGTCGGAGCGGG
  TGTCCGGCCTGGCCGGCTCCATCTACCGCGAGTTCGAGCGGCTTATCGGGCGCTATGACGAGGAGGTGGT
  CAAAGAGCTGATGCCGCTGGTGGTGGCTGTGCTGGAGAACCTGGACTCGGTGTTCGCGCAGGACCAGGAG
  CACCAGGTGGAGCTGGAGCTGCTGCGGGACGACAACGAGCAGCTCATCACCCAGTACGAGCGGGAGAAGG
  CGCTGCGCAAGCACGCTGAGGAGAAATTCATTGAATTTGAAGACTCTCAAGAACAGGAAAAAAAGGACTT
  ACAGACCCGAGTGGAATCTTTAGAATCTCAAACAAGACAACTTGAGCTGAAAGCGAAAAACTATGCTGAC
  CAGATTAGCAGACTTGAAGAAAGAGAAGCAGAACTGAAGAAGGAATATAATGCATTACATCAAAGACACA
  CTGAGATGATCCATAATTATATGGAACATTTAGAAAGAACAAAACTTCATCAGCTCTCAGGGAGTGATCA
  ACTAGAATCCACAGCTCATAGTAGAATTAGAAAAGAACGCCCTATATCATTAGGAATTTTCCCATTACCT
  GCTGGAGATGGATTGCTTACACCTGATGCTCAGAAAGGAGGAGAGACCCCTGGATCTGAGCAATGGAAAT
  TTCAGGAATTAAGTCAACCACGTTCTCATACCAGCCTGAAGGATGAGCTTTCTGATGTTAGCCAAGGCGG
  ATCTAAAGCTACCACTCCAGCATCAACAGCTAATTCAGATGTGGCAACAATTCCTACTGATACTCCCTTA
  AAGGAAGAAAACGAAGGATTTGTGAAGGTTACAGATGCGCCAAATAAATCAGAGATAAGCAAACACATTG
  AAGTACAGGTAGCCCAGGAAACTAGAAATGTATCTACTGGCTCTGCTGAAAATGAAGAAAAGTCAGAAGT
  TCAAGCAATCATCGAATCTACTCCTGAGCTGGATATGGACAAAGATCTCAGTGGATATAAAGGTTCAAGC
  ACTCCCACCAAAGGCATAGAGAACAAAGCTTTTGATCGCAATACAGAATCTCTCTTTGAAGAACTGTCTT
  CAGCTGGCTCAGGCCTAATAGGAGATGTGGATGAAGGAGCAGATTTACTAGGAATGGGTCGGGAAGTTGA
  GAATCTTATATTAGAAAATACACAACTGTTGGAAACCAAAAATGCTTTGAACATAGTGAAGAATGATTTG
  ATAGCAAAAGTGGATGAACTGACCTGTGAGAAAGATGTGCTGCAAGGGGAATTGGAGGCTGTGAAGCAAG
  CCAAACTGAAACTAGAGGAAAAGAACAGAGAATTGGAGGAAGAGCTTAGGAAAGCTCGGGCAGAAGCTGA
  AGATGCAAGGCAAAAAGCAAAAGATGACGATGATAGTGATATTCCCACAGCCCAGAGGAAACGGTTTACT
  AGAGTAGAAATGGCCCGTGTTCTCATGGAGCGAAACCAGTATAAAGAGAGATTGATGGAGCTTCAGGAAG
  CTGTTCGATGGACAGAGATGATTCGGGCATCACGAGAAAATCCAGCCATGCAGGAAAAAAAAAGGTCAAG
  CATTTGGCAGTTTTTCAGCCGACTTTTCAGCTCCTCAAGTAACACGACTAAGAAGCCTGAACCACCTGTT
  AATCTGAAGTACAATGCACCCACGTCTCATGTTACTCCGTCCGTCAAGAAAAGAAGCAGCACCTTATCTC
  AGCTCCCTGGGGATAAGTCCAAAGCCTTTGATTTCCTTAGTGAAGAAACTGAAGCTAGTTTAGCCTCACG
  CAGAGAACAAAAGAGAGAGCAGTATCGTCAGGTAAAAGCACATGTTCAGAAGGAAGACGGTAGAGTGCAG
  GCTTTTGGCTGGAGTCTGCCTCAGAAGTACAAACAGGTAACCAATGGTCAAGGTGAAAATAAGATGAAAA
  ATTTACCTGTGCCTGTCTATCTCAGACCTCTGGATGAAAAAGATACATCAATGAAGCTGTGGTGTGCTGT
  TGGAGTCAATTTATCTGGTGGGAAGACCAGAGATGGTGGTTCTGTTGTTGGAGCAAGTGTATTTTACAAG
  GATGTTGCTGGTTTGGATACAGAAGGCAGTAAACAGCGAAGTGCCTCTCAGAGTAGTTTAGATAAGTTAG
  ATCAGGAACTTAAGGAACAGCAGAAGGAGTTAAAAAATCAAGAAGAATTATCCAGTCTAGTTTGGATCTG
  TACCAGCACTCATTCGGCTACAAAAGTTCTTATTATTGATGCTGTTCAACCTGGCAACATCCTAGACAGT
  TTCACTGTTTGCAACTCTCATGTTCTGTGCATTGCAAGTGTGCCAGGTGCACGAGAAACAGACTACCCTG
  CAGGAGAAGATCTTTCAGAATCTGGTCAGGTAGACAAAGCATCTTTATGTGGAAGTATGACAAGCAACAG
  CTCAGCAGAGACAGACAGCCTGTTAGGAGGCATCACAGTGGTTGGTTGTTCTGCAGAAGGTGTGACGGGA
  GCTGCCACTTCCCCTAGTACAAATGGTGCTTCTCCAGTGATGGATAAACCACCAGAAATGGAAGCAGAAA
  ATAGTGAGGTTGATGAAAATGTTCCAACAGCAGAAGAAGCAACTGAAGCTACAGAAGGGAATGCGGGGTC
  AGCTGAAGACACAGTGGACATCTCCCAAACTGGCGTCTACACAGAGCATGTCTTTACAGATCCTTTGGGA
  GTTCAGATCCCAGAAGACCTCTCCCCAGTGTATCAGTCGAGCAATGACTCAGATGCATATAAAGATCAAA
  TATCAGTACTGCCAAATGAACAAGACTTGGTGAGAGAAGAAGCCCAGAAAATGAGTAGTCTTTTACCAAC
  TATGTGGCTTGGAGCTCAAAATGGCTGTTTGTATGTCCATTCATCTGTAGCCCAGTGGAGGAAATGTCTC
  CATTCCATTAAACTTAAAGATTCGATTCTCAGTATTGTACACGTGAAGGGAATCGTGTTAGTAGCCCTGG
  CTGACGGCACCCTTGCAATCTTTCACAGAGGAGTGGATGGGCAGTGGGATTTGTCAAACTATCACCTCTT
  AGACCTTGGACGGCCTCATCATTCCATCCGTTGCATGACTGTGGTACATGACAAAGTCTGGTGTGGCTAT
  AGGAACAAAATCTATGTGGTGCAGCCAAAGGCCATGAAAATAGAGAAATCTTTTGATGCACATCCCAGGA
  AGGAGAGCCAAGTGCGACAGCTTGCGTGGGTGGGGGATGGCGTGTGGGTCTCCATTCGCTTGGATTCTAC
  GCTCCGTCTCTATCATGCACACACTTATCAACATCTACAGGATGTGGACATTGAGCCTTATGTAAGCAAA
  ATGTTAGGTACTGGAAAACTGGGCTTCTCTTTTGTGAGAATTACAGCTCTTATGGTGTCTTGTAATCGTT
  TGTGGGTGGGGACAGGAAATGGTGTCATTATCTCCATCCCATTGACAGAAACAAATAAAACCTCAGGTGT
  ACCAGGAAATCGTCCTGGAAGTGTAATCCGTGTATATGGTGATGAAAACAGTGATAAAGTGACTCCAGGG
  ACATTTATACCCTATTGTTCAATGGCACATGCACAGCTTTGCTTCCATGGGCACCGGGATGCTGTGAAAT
  TCTTTGTGGCAGTCCCAGGTCAAGTCATCAGCCCACAAAGTAGCAGTAGTGGCACGGATCTGACGGGTGA
  CAAAGCAGGGCCATCTGCACAGGAGCCTGGTAGTCAGACGCCCTTGAAGTCTATGCTTGTCATCAGTGGA
  GGAGAGGGCTACATCGACTTCCGAATGGGTGATGAAGGTGGAGAATCAGAACTTCTTGGAGAGGATCTTC
  CACTTGAACCTTCTGTCACCAAAGCAGAAAGGAGTCACTTGATAGTGTGGCAAGTGATGTATGGCAATGA
  G
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217252 representing NM_003971
  Red=Cloning site Green=Tags(s)
  
  MELEDGVVYQEEPGGSGAVMSERVSGLAGSIYREFERLIGRYDEEVVKELMPLVVAVLENLDSVFAQDQE
  HQVELELLRDDNEQLITQYEREKALRKHAEEKFIEFEDSQEQEKKDLQTRVESLESQTRQLELKAKNYAD
  QISRLEEREAELKKEYNALHQRHTEMIHNYMEHLERTKLHQLSGSDQLESTAHSRIRKERPISLGIFPLP
  AGDGLLTPDAQKGGETPGSEQWKFQELSQPRSHTSLKDELSDVSQGGSKATTPASTANSDVATIPTDTPL
  KEENEGFVKVTDAPNKSEISKHIEVQVAQETRNVSTGSAENEEKSEVQAIIESTPELDMDKDLSGYKGSS
  TPTKGIENKAFDRNTESLFEELSSAGSGLIGDVDEGADLLGMGREVENLILENTQLLETKNALNIVKNDL
  IAKVDELTCEKDVLQGELEAVKQAKLKLEEKNRELEEELRKARAEAEDARQKAKDDDDSDIPTAQRKRFT
  RVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENPAMQEKKRSSIWQFFSRLFSSSSNTTKKPEPPV
  NLKYNAPTSHVTPSVKKRSSTLSQLPGDKSKAFDFLSEETEASLASRREQKREQYRQVKAHVQKEDGRVQ
  AFGWSLPQKYKQVTNGQGENKMKNLPVPVYLRPLDEKDTSMKLWCAVGVNLSGGKTRDGGSVVGASVFYK
  DVAGLDTEGSKQRSASQSSLDKLDQELKEQQKELKNQEELSSLVWICTSTHSATKVLIIDAVQPGNILDS
  FTVCNSHVLCIASVPGARETDYPAGEDLSESGQVDKASLCGSMTSNSSAETDSLLGGITVVGCSAEGVTG
  AATSPSTNGASPVMDKPPEMEAENSEVDENVPTAEEATEATEGNAGSAEDTVDISQTGVYTEHVFTDPLG
  VQIPEDLSPVYQSSNDSDAYKDQISVLPNEQDLVREEAQKMSSLLPTMWLGAQNGCLYVHSSVAQWRKCL
  HSIKLKDSILSIVHVKGIVLVALADGTLAIFHRGVDGQWDLSNYHLLDLGRPHHSIRCMTVVHDKVWCGY
  RNKIYVVQPKAMKIEKSFDAHPRKESQVRQLAWVGDGVWVSIRLDSTLRLYHAHTYQHLQDVDIEPYVSK
  MLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLTETNKTSGVPGNRPGSVIRVYGDENSDKVTPG
  TFIPYCSMAHAQLCFHGHRDAVKFFVAVPGQVISPQSSSSGTDLTGDKAGPSAQEPGSQTPLKSMLVISG
  GEGYIDFRMGDEGGESELLGEDLPLEPSVTKAERSHLIVWQVMYGNE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003971
• ORF Size: 3921 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_003971.3, NP_003962.3
• RefSeq Size: 4663
• RefSeq ORF: 3924
• Locus ID: 9043
• Gene Summary: This gene encodes a member of the cancer testis antigen gene family. The encoded protein functions as a scaffold protein that structurally organizes mitogen-activated protein kinases and mediates c-Jun-terminal kinase signaling. This protein also binds to kinesin-1 and may be involved in microtubule-based membrane transport. This protein may play a role in tumor growth and development. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2011]