L2HGDH (NM_024884) Human Tagged ORF Clone

CAT#: RG217631

L2HGDH (GFP-tagged) - Human L-2-hydroxyglutarate dehydrogenase (L2HGDH), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: L2HGDH
• Synonyms: C14orf160; L2HGA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217631 representing NM_024884
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCCAGCGCTGCGTTATTTGGTTGGTGCCTGCGGACGGGCCCGCGGGCTTTTCGCCGGTGGCTCCC
  CTGGGGCGTGCGGGTTCGCGTCTGGGAGGCCAAGACCGCTGTGTGGAGGTAGCCGCAGCGCCAGCACCAG
  CTCATTTGATATAGTCATCGTTGGTGGCGGAATTGTGGGGCTTGCCTCTGCCAGAGCACTCATCCTGCGA
  CATCCATCACTTTCTATTGGTGTTCTGGAAAAGGAGAAAGATTTAGCTGTTCACCAGACTGGACATAACA
  GTGGTGTCATACATAGTGGAATTTATTATAAACCTGAGTCTCTGAAAGCCAAATTATGTGTACAAGGTGC
  AGCCCTCCTCTATGAGTACTGTCAGCAAAAGGGGATTTCCTACAAGCAGTGTGGCAAGCTTATAGTAGCT
  GTTGAACAAGAAGAAATTCCCAGACTTCAGGCCCTATATGAGAAAGGCCTCCAGAATGGTGTCCCGGGCC
  TGAGGCTGATCCAGCAGGAGGATATAAAAAAGAAGGAGCCATATTGTAGGGGTCTAATGGCTATTGATTG
  TCCACATACTGGCATTGTGGACTATCGGCAGGTGGCTTTGTCATTTGCCCAGGATTTCCAAGAAGCAGGT
  GGCTCTGTCTTGACCAATTTTGAAGTAAAAGGTATTGAAATGGCTAAAGAAAGTCCTTCAAGAAGTATAG
  ATGGAATGCAATATCCAATTGTTATAAAGAATACAAAGGGAGAGGAAATTCGATGTCAGTATGTTGTGAC
  ATGTGCAGGACTTTACTCAGACCGTATTTCAGAGTTGAGTGGCTGCACTCCTGATCCTCGAATTGTACCA
  TTCCGGGGAGATTACCTGCTTTTGAAGCCAGAAAAATGTTATCTTGTAAAAGGAAATATTTATCCGGTCC
  CAGATAGCCGGTTTCCTTTCCTAGGAGTTCACTTCACACCAAGGATGGATGGCAGTATTTGGCTAGGGCC
  TAATGCAGTTCTTGCCTTTAAACGAGAGGGTTACAGACCCTTTGACTTCAGTGCCACAGATGTTATGGAT
  ATAATTATCAATAGTGGCTTGATTAAACTGGCATCCCAGAATTTTTCCTATGGAGTTACTGAAATGTATA
  AAGCATGTTTTCTTGGTGCAACAGTGAAGTATCTTCAAAAATTCATCCCTGAAATTACTATCAGTGATAT
  ACTTAGGGGCCCAGCTGGAGTAAGAGCCCAGGCCCTGGATAGAGATGGAAATCTGGTAGAAGATTTTGTA
  TTTGATGCAGGAGTTGGGGATATTGGAAATCGCATTCTTCATGTGAGAAATGCACCTTCTCCTGCTGCTA
  CTTCTTCCATTGCAATTTCTGGAATGATTGCAGATGAAGTACAACAAAGATTTGAATTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217631 representing NM_024884
  Red=Cloning site Green=Tags(s)
  
  MVPALRYLVGACGRARGLFAGGSPGACGFASGRPRPLCGGSRSASTSSFDIVIVGGGIVGLASARALILR
  HPSLSIGVLEKEKDLAVHQTGHNSGVIHSGIYYKPESLKAKLCVQGAALLYEYCQQKGISYKQCGKLIVA
  VEQEEIPRLQALYEKGLQNGVPGLRLIQQEDIKKKEPYCRGLMAIDCPHTGIVDYRQVALSFAQDFQEAG
  GSVLTNFEVKGIEMAKESPSRSIDGMQYPIVIKNTKGEEIRCQYVVTCAGLYSDRISELSGCTPDPRIVP
  FRGDYLLLKPEKCYLVKGNIYPVPDSRFPFLGVHFTPRMDGSIWLGPNAVLAFKREGYRPFDFSATDVMD
  IIINSGLIKLASQNFSYGVTEMYKACFLGATVKYLQKFIPEITISDILRGPAGVRAQALDRDGNLVEDFV
  FDAGVGDIGNRILHVRNAPSPAATSSIAISGMIADEVQQRFEL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024884
• ORF Size: 1389 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_024884.1, NP_079160.1
• RefSeq Size: 2064
• RefSeq ORF: 1392
• Locus ID: 79944
• Protein Pathways: Butanoate metabolism
• Gene Summary: This gene encodes L-2-hydroxyglutarate dehydrogenase, a FAD-dependent enzyme that oxidizes L-2-hydroxyglutarate to alpha-ketoglutarate in a variety of mammalian tissues. Mutations in this gene cause L-2-hydroxyglutaric aciduria, a rare autosomal recessive neurometabolic disorder resulting in moderate to severe cognitive disability. [provided by RefSeq, Jul 2008]