USP2 (NM_171997) Human Tagged ORF Clone

CAT#: RG217695

USP2 (GFP-tagged) - Human ubiquitin specific peptidase 2 (USP2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: USP2
• Synonyms: UBP41; USP9
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217695 representing NM_171997
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCACCTCGTACACCGTGACCCTGCCCGAGGACCCCCCCGCCGCCCCCTTTCCCGCCCTCGCCAAGG
  AGCTGCGGCCGCGCTCCCCTCTCTCCCCGTCCCTGCTGCTCTCCACCTTCGTGGGGCTCCTGCTCAACAA
  AGCCAAGAATTCTAAGAGTGCCCAGGGTCTGGCTGGTCTTCGAAACCTTGGGAACACGTGCTTCATGAAC
  TCAATTCTGCAGTGCCTGAGCAACACTCGGGAGTTGAGAGATTACTGCCTCCAGAGGCTCTACATGCGGG
  ACCTGCACCACGGCAGCAATGCACACACAGCCCTCGTGGAAGAGTTTGCAAAACTAATTCAGACCATATG
  GACTTCATCCCCCAATGATGTGGTGAGCCCATCTGAGTTCAAGACCCAGATCCAGAGATACGCACCGCGC
  TTTGTTGGCTATAATCAGCAGGATGCTCAGGAGTTCCTTCGCTTTCTTCTGGATGGGCTCCATAACGAGG
  TGAACCGAGTGACACTGAGACCTAAGTCCAACCCTGAGAACCTCGATCATCTTCCTGATGACGAGAAAGG
  CCGACAGATGTGGAGAAAATATCTAGAACGGGAAGACAGTAGGATCGGGGATCTCTTTGTTGGGCAGCTA
  AAGAGCTCGCTGACGTGTACAGATTGTGGTTACTGTTCTACGGTCTTCGACCCCTTCTGGGACCTCTCAC
  TGCCCATTGCTAAGCGAGGTTATCCTGAGGTGACATTAATGGACTGCATGAGGCTCTTCACCAAAGAGGA
  TGTGCTTGATGGAGATGAAAAGCCAACATGCTGTCGCTGCCGAGGCAGAAAACGGTGTATAAAGAAGTTC
  TCCATCCAGAGGTTCCCAAAGATCTTGGTGCTCCATCTGAAGCGGTTCTCAGAATCCAGGATCCGAACCA
  GCAAGCTCACAACATTTGTGAACTTCCCCCTAAGAGACCTGGACTTAAGAGAATTTGCCTCAGAAAACAC
  CAACCATGCTGTTTACAACCTGTACGCTGTGTCCAATCACTCCGGAACCACCATGGGTGGCCACTATACA
  GCCTACTGTCGCAGTCCAGGGACAGGAGAATGGCACACTTTCAACGACTCCAGCGTCACTCCCATGTCCT
  CCAGCCAAGTGCGCACCAGCGACGCCTACCTGCTCTTCTACGAACTGGCCAGCCCGCCCTCCCGAATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217695 representing NM_171997
  Red=Cloning site Green=Tags(s)
  
  MRTSYTVTLPEDPPAAPFPALAKELRPRSPLSPSLLLSTFVGLLLNKAKNSKSAQGLAGLRNLGNTCFMN
  SILQCLSNTRELRDYCLQRLYMRDLHHGSNAHTALVEEFAKLIQTIWTSSPNDVVSPSEFKTQIQRYAPR
  FVGYNQQDAQEFLRFLLDGLHNEVNRVTLRPKSNPENLDHLPDDEKGRQMWRKYLEREDSRIGDLFVGQL
  KSSLTCTDCGYCSTVFDPFWDLSLPIAKRGYPEVTLMDCMRLFTKEDVLDGDEKPTCCRCRGRKRCIKKF
  SIQRFPKILVLHLKRFSESRIRTSKLTTFVNFPLRDLDLREFASENTNHAVYNLYAVSNHSGTTMGGHYT
  AYCRSPGTGEWHTFNDSSVTPMSSSQVRTSDAYLLFYELASPPSRM
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_171997
• ORF Size: 1188 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_171997.1, NP_741994.1
• RefSeq Size: 1378
• RefSeq ORF: 1191
• Locus ID: 9099
• Protein Families: Protease
• Gene Summary: This gene encodes a member of the family of de-ubiquitinating enzymes, which belongs to the peptidase C19 superfamily. The encoded protein is a ubiquitin-specific protease which is required for TNF-alpha (tumor necrosis factor alpha) -induced NF-kB (nuclear factor kB) signaling. This protein deubiquitinates polyubiquitinated target proteins such as fatty acid synthase, murine double minute 2 (MDM2), MDM4/MDMX and cyclin D1. MDM2 and MDM4 are negative regulators of the p53 tumor suppressor and cyclin D1 is required for cell cycle G1/S transition. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]