GLEPP1 (PTPRO) (NM_002848) Human Tagged ORF Clone

CAT#: RG217755

PTPRO (GFP-tagged) - Human protein tyrosine phosphatase, receptor type, O (PTPRO), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PTPRO
• Synonyms: GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT; PTPU2; R-PTP-O
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217755 representing NM_002848
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCACCTGCCCACGGGGATACACGGCGCCCGCCGCCTCCTGCCTCTGCTCTGGCTCTTTGTGCTGT
  TCAAGAATGCTACAGCTTTCCATGTAACTGTCCAAGATGATAATAACATCGTTGTCTCATTAGAAGCTTC
  AGACGTCATCAGTCCAGCATCTGTGTATGTTGTGAAGATAACTGGTGAATCCAAAAATTATTTCTTCGAA
  TTTGAGGAATTCAACAGCACTTTGCCTCCTCCTGTTATTTTCAAGGCCAGTTATCATGGCCTTTATTATA
  TAATCACTCTGGTAGTGGTAAATGGAAATGTGGTGACCAAGCCATCCAGATCAATCACTGTGTTAACAAA
  ACCTCTACCTGTAACCAGTGTTTCCATATATGACTATAAACCTTCTCCTGAAACAGGAGTCCTGTTTGAA
  ATACATTATCCAGAAAAATATAACGTTTTCACAAGAGTGAACATTAGCTACTGGGAAGGTAAAGACTTCC
  GGACAATGCTATATAAAGATTTCTTTAAGGGAAAAACAGTATTTAATCACTGGCTGCCAGGAATGTGTTA
  TAGTAATATCACCTTTCAGCTGGTATCTGAGGCAACTTTTAATAAAAGTACCCTTGTTGAGTACAGTGGT
  GTCAGTCACGAACCCAAACAGCACAGAACTGCCCCTTATCCACCTCAAAATATTTCCGTTCGTATCGTAA
  ACTTGAACAAAAACAACTGGGAAGAACAGAGTGGCAATTTCCCAGAAGAATCCTTCATGAGATCACAAGA
  TACAATAGGAAAAGAAAAACTCTTCCATTTTACAGAAGAAACCCCTGAAATTCCCTCGGGCAACATTTCT
  TCCGGTTGGCCTGATTTTAATAGCAGTGACTATGAAACTACGTCTCAGCCATATTGGTGGGACAGTGCAT
  CTGCAGCTCCTGAAAGTGAAGATGAATTTGTCAGCGTACTTCCCATGGAATACGAAAATAACAGTACACT
  CAGTGAGACAGAGAAGTCAACATCAGGCTCTTTCTCCTTTTTCCCTGTGCAAATGATATTGACCTGGTTA
  CCACCCAAACCACCCACTGCTTTTGATGGGTTCCATATCCATATTGAACGAGAAGAGAACTTTACTGAAT
  ATTTGATGGTGGATGAAGAAGCACATGAATTTGTTGCAGAACTGAAGGAACCTGGGAAATATAAGTTATC
  TGTGACAACCTTTAGTTCCTCAGGATCTTGTGAAACTCGAAAAAGTCAGTCAGCAAAATCACTCAGCTTT
  TATATCAGTCCTTCAGGAGAGTGGATTGAAGAACTGACCGAGAAGCCGCAGCACGTGAGTGTCCACGTTT
  TAAGCTCAACCACTGCCTTGATGTCCTGGACATCTTCCCAAGAGAACTACAACAGCACCATTGTGTCTGT
  GGTGTCGCTGACCTGCCAGAAACAAAAGGAGAGCCAGAGGCTTGAAAAGCAGTACTGCACTCAGGTGAAC
  TCAAGCAAACCTATTATTGAAAATCTGGTTCCTGGTGCCCAGTACCAGGTTGTAATATACCTAAGGAAAG
  GCCCTTTGATTGGACCACCTTCAGATCCTGTGACATTTGCTATTGTTCCCACAGGAATAAAGGATTTAAT
  GCTCTATCCTTTGGGTCCTACGGCCGTGGTTCTGAGCTGGACCAGACCTTATTTAGGCGTGTTCAGAAAA
  TACGTGGTTGAAATGTTTTATTTCAACCCTGCTACAATGACATCAGAGTGGACCACCTACTATGAAATAG
  CAGCAACTGTTTCCTTAACTGCATCCGTGAGAATAGCTAATCTGCTGCCAGCATGGTACTACAACTTCCG
  GGTTACCATGGTGACGTGGGGAGATCCAGAATTGAGCTGCTGTGACAGCTCTACCATCAGCTTCATAACA
  GCCCCAGTGGCTCCGGAAATCACTTCTGTGGAATATTTCAACAGTCTGTTATATATCAGTTGGACATATG
  GGGATGATACAACGGACTTGTCCCATTCTAGAATGCTTCACTGGATGGTGGTTGCAGAAGGAAAAAAGAA
  AATTAAAAAGAGTGTAACACGCAATGTCATGACTGCAATTCTCAGCTTGCCTCCAGGCGACATCTATAAC
  CTCTCAGTAACTGCTTGTACTGAAAGAGGAAGTAATACCTCCATGCTCCGCCTTGTCAAGCTAGAACCAG
  CTCCACCCAAATCACTCTTCGCAGTGAACAAAACCCAGACTTCAGTGACTTTGCTGTGGGTGGAAGAGGG
  AGTAGCTGATTTCTTTGAAGTTTTCTGTCAACAAGTTGGCTCCAGTCAGAAAACCAAACTTCAGGAACCA
  GTTGCTGTTTCTTCCCATGTCGTGACCATCTCCAGCCTTCTTCCTGCCACTGCCTACAATTGTAGTGTCA
  CCAGCTTTAGCCATGACAGCCCCAGTGTCCCTACGTTCATAGCCGTCTCAACAATGGTTACAGAGATGAA
  TCCCAATGTGGTAGTGATCTCCGTGCTGGCCATCCTTAGCACACTTTTAATTGGACTGTTGCTTGTTACC
  CTCATTATTCTTAGGAAAAAGCATCTGCAGATGGCTAGGGAGTGTGGAGCTGGTACATTTGTCAATTTTG
  CATCCTTAGAGAGGGATGGAAAGCTTCCATACAACTGGAGTAAAAATGGTTTAAAGAAGAGGAAACTGAC
  AAACCCGGTTCAACTGGATGACTTTGATGCCTATATTAAGGATATGGCCAAAGACTCTGACTATAAATTT
  TCTCTTCAGTTTGAGGAGTTGAAATTGATTGGACTGGATATCCCACACTTTGCTGCAGATCTTCCACTGA
  ATCGATGTAAAAACCGTTACACAAACATCCTACCATATGACTTCAGCCGTGTGAGATTAGTCTCCATGAA
  TGAAGAGGAAGGTGCAGACTACATCAATGCCAACTATATTCCTGGATACAACTCACCCCAGGAGTATATT
  GCCACCCAGGGGCCACTGCCTGAAACCAGAAATGACTTCTGGAAGATGGTCCTGCAACAAAAGTCTCAGA
  TTATTGTCATGCTCACTCAGTGTAATGAGAAAAGGAGGGTGAAATGTGACCATTACTGGCCATTCACGGA
  AGAACCTATAGCCTATGGAGACATCACTGTGGAGATGATTTCAGAGGAAGAGCAGGACGACTGGGCCTGT
  AGACACTTCCGGATCAACTATGCTGACGAGATGCAGGATGTGATGCATTTTAACTACACTGCATGGCCTG
  ATCATGGTGTGCCCACAGCAAATGCTGCAGAAAGTATCCTGCAGTTTGTACACATGGTCCGACAGCAAGC
  TACCAAGAGCAAAGGTCCCATGATCATTCACTGCAGTGCTGGCGTGGGACGGACAGGAACATTCATTGCC
  CTGGACAGGCTCTTGCAGCACATTCGGGATCATGAGTTTGTTGACATCTTAGGGCTGGTGTCAGAAATGA
  GGTCATACCGGATGTCTATGGTACAGACAGAGGAGCAGTACATTTTTATCCATCAGTGTGTGCAACTGAT
  GTGGATGAAGAAGAAGCAGCAGTTCTGCATCAGTGATGTCATATACGAGAATGTTAGCAAGTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217755 representing NM_002848
  Red=Cloning site Green=Tags(s)
  
  MGHLPTGIHGARRLLPLLWLFVLFKNATAFHVTVQDDNNIVVSLEASDVISPASVYVVKITGESKNYFFE
  FEEFNSTLPPPVIFKASYHGLYYIITLVVVNGNVVTKPSRSITVLTKPLPVTSVSIYDYKPSPETGVLFE
  IHYPEKYNVFTRVNISYWEGKDFRTMLYKDFFKGKTVFNHWLPGMCYSNITFQLVSEATFNKSTLVEYSG
  VSHEPKQHRTAPYPPQNISVRIVNLNKNNWEEQSGNFPEESFMRSQDTIGKEKLFHFTEETPEIPSGNIS
  SGWPDFNSSDYETTSQPYWWDSASAAPESEDEFVSVLPMEYENNSTLSETEKSTSGSFSFFPVQMILTWL
  PPKPPTAFDGFHIHIEREENFTEYLMVDEEAHEFVAELKEPGKYKLSVTTFSSSGSCETRKSQSAKSLSF
  YISPSGEWIEELTEKPQHVSVHVLSSTTALMSWTSSQENYNSTIVSVVSLTCQKQKESQRLEKQYCTQVN
  SSKPIIENLVPGAQYQVVIYLRKGPLIGPPSDPVTFAIVPTGIKDLMLYPLGPTAVVLSWTRPYLGVFRK
  YVVEMFYFNPATMTSEWTTYYEIAATVSLTASVRIANLLPAWYYNFRVTMVTWGDPELSCCDSSTISFIT
  APVAPEITSVEYFNSLLYISWTYGDDTTDLSHSRMLHWMVVAEGKKKIKKSVTRNVMTAILSLPPGDIYN
  LSVTACTERGSNTSMLRLVKLEPAPPKSLFAVNKTQTSVTLLWVEEGVADFFEVFCQQVGSSQKTKLQEP
  VAVSSHVVTISSLLPATAYNCSVTSFSHDSPSVPTFIAVSTMVTEMNPNVVVISVLAILSTLLIGLLLVT
  LIILRKKHLQMARECGAGTFVNFASLERDGKLPYNWSKNGLKKRKLTNPVQLDDFDAYIKDMAKDSDYKF
  SLQFEELKLIGLDIPHFAADLPLNRCKNRYTNILPYDFSRVRLVSMNEEEGADYINANYIPGYNSPQEYI
  ATQGPLPETRNDFWKMVLQQKSQIIVMLTQCNEKRRVKCDHYWPFTEEPIAYGDITVEMISEEEQDDWAC
  RHFRINYADEMQDVMHFNYTAWPDHGVPTANAAESILQFVHMVRQQATKSKGPMIIHCSAGVGRTGTFIA
  LDRLLQHIRDHEFVDILGLVSEMRSYRMSMVQTEEQYIFIHQCVQLMWMKKKQQFCISDVIYENVSKS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002848
• ORF Size: 6292 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002848.2, NP_002839.1
• RefSeq Size: 5069 bp
• RefSeq ORF: 3567 bp
• Locus ID: 5800
• Cytogenetics: 12p13-p12
• Domains: Y_phosphatase, PTPc_motif, FN3
• Protein Families: Phosphatase, Transmembrane
• Gene Summary: 'This gene encodes a member of the R3 subtype family of receptor-type protein tyrosine phosphatases. These proteins are localized to the apical surface of polarized cells and may have tissue-specific functions through activation of Src family kinases. This gene contains two distinct promoters, and alternatively spliced transcript variants encoding multiple isoforms have been observed. The encoded proteins may have multiple isoform-specific and tissue-specific functions, including the regulation of osteoclast production and activity, inhibition of cell proliferation and facilitation of apoptosis. This gene is a candidate tumor suppressor, and decreased expression of this gene has been observed in several types of cancer. [provided by RefSeq, May 2011]'