MEGF10 (NM_032446) Human Tagged ORF Clone

CAT#: RG217883

MEGF10 (GFP-tagged) - Human multiple EGF-like-domains 10 (MEGF10)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MEGF10
• Synonyms: EMARDD
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217883 representing NM_032446
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTATTTCTTTGAACTCATGCCTGAGCTTTATTTGTTTATTGTTATGCCACTGGATTGGGACAGCAT
  CACCTCTGAATCTTGAAGACCCTAATGTGTGTAGCCACTGGGAAAGCTACTCAGTGACTGTGCAAGAGTC
  ATACCCACATCCCTTTGATCAAATTTACTACACGAGCTGCACTGACATTCTAAACTGGTTTAAATGCACG
  CGGCACAGAGTCAGCTATCGGACAGCCTATCGACATGGGGAGAAGACTATGTATAGGCGCAAGTCTCAGT
  GTTGTCCTGGATTTTATGAAAGCGGGGAAATGTGTGTCCCCCACTGTGCTGATAAATGTGTCCATGGTCG
  CTGTATTGCTCCAAACACCTGTCAGTGTGAGCCTGGCTGGGGAGGGACCAACTGCTCCAGTGCCTGCGAT
  GGTGATCACTGGGGTCCCCACTGCACCAGCCGGTGCCAGTGCAAAAATGGGGCTCTGTGCAACCCCATCA
  CCGGGGCTTGCCACTGTGCTGCGGGCTTCCGGGGCTGGCGCTGCGAGGACCGCTGTGAGCAGGGCACCTA
  TGGTAACGACTGTCATCAGAGATGCCAGTGCCAGAATGGAGCCACCTGCGACCACGTCACGGGGGAATGC
  CGCTGCCCACCAGGATACACCGGAGCCTTCTGTGAGGATCTTTGTCCTCCTGGTAAACATGGTCCACAGT
  GTGAGCAGAGATGCCCTTGTCAAAATGGAGGAGTGTGTCATCACGTCACTGGAGAATGCTCTTGCCCTTC
  TGGCTGGATGGGCACAGTGTGTGGTCAGCCTTGCCCCGAGGGTCGCTTTGGAAAGAACTGTTCCCAAGAA
  TGCCAGTGCCATAATGGAGGGACGTGTGATGCTGCCACAGGCCAATGTCATTGCAGTCCAGGATACACAG
  GGGAACGGTGCCAGGATGAGTGTCCTGTTGGGACCTATGGCGTTCTCTGTGCTGAGACCTGCCAGTGTGT
  CAACGGAGGGAAGTGTTACCACGTGAGCGGCGCATGCCTCTGTGAAGCAGGCTTTGCTGGCGAGCGCTGC
  GAAGCACGCCTGTGTCCTGAGGGGCTCTACGGCATCAAATGTGACAAACGGTGTCCCTGCCACTTGGAAA
  ACACTCATAGCTGTCACCCCATGTCTGGAGAGTGTGCCTGCAAGCCGGGCTGGTCAGGACTCTACTGTAA
  TGAGACATGTTCTCCTGGATTCTACGGGGAAGCTTGCCAGCAGATCTGCAGCTGCCAAAATGGGGCAGAC
  TGTGACAGTGTGACTGGAAAGTGCACCTGTGCCCCAGGATTCAAAGGAATTGACTGCTCTACCCCATGCC
  CTCTGGGAACCTATGGGATAAACTGTTCCTCTCGCTGTGGCTGTAAAAATGATGCAGTCTGCTCTCCTGT
  GGACGGGTCTTGTACTTGCAAGGCAGGCTGGCACGGGGTGGACTGCTCCATCAGATGTCCCAGTGGCACA
  TGGGGCTTTGGCTGTAACTTAACATGCCAGTGCCTCAACGGGGGAGCCTGCAACACCCTGGACGGGACCT
  GCACGTGTGCACCTGGATGGCGCGGGGAGAAATGCGAACTTCCCTGCCAGGATGGCACGTACGGGCTGAA
  CTGTGCTGAGCGCTGCGACTGCAGCCACGCAGATGGCTGCCACCCTACCACGGGCCATTGCCGCTGCCTC
  CCCGGATGGTCAGGTGTCCACTGTGACAGCGTGTGTGCTGAGGGACGCTGGGGCCCCAACTGCTCCCTGC
  CCTGCTACTGTAAAAATGGGGCTTCATGCTCCCCTGATGATGGCATCTGCGAGTGTGCACCAGGCTTCCG
  AGGCACCACTTGTCAGAGGATCTGCTCCCCTGGTTTTTATGGGCATCGCTGCAGCCAGACATGCCCACAG
  TGCGTTCACAGCAGCGGGCCCTGCCACCACATCACCGGCCTGTGTGACTGCTTGCCTGGCTTCACAGGCG
  CCCTCTGCAATGAAGTGTGTCCCAGTGGCAGATTTGGGAAAAACTGTGCAGGAATTTGTACCTGCACCAA
  CAACGGAACCTGTAACCCCATTGACAGATCTTGTCAGTGTTACCCCGGTTGGATTGGCAGTGACTGCTCT
  CAACCATGTCCACCTGCCCACTGGGGCCCAAACTGCATCCACACGTGCAACTGCCATAATGGAGCTTTCT
  GCAGCGCCTACGATGGGGAATGTAAATGCACTCCTGGCTGGACAGGGCTCTACTGCACTCAGAGATGTCC
  TCTAGGGTTTTATGGAAAAGATTGTGCACTGATATGCCAATGTCAAAACGGAGCTGACTGCGACCACATT
  TCTGGGCAGTGTACTTGCCGCACTGGATTCATGGGACGGCACTGTGAGCAGAAGTGCCCTTCAGGAACAT
  ATGGCTATGGCTGTCGCCAGATATGTGATTGTCTGAACAACTCCACCTGCGACCACATCACTGGGACCTG
  TTACTGCAGCCCCGGATGGAAGGGAGCGAGATGTGATCAAGCTGGTGTTATCATAGTTGGAAATCTGAAC
  AGCTTAAGCCGAACCAGTACTGCTCTCCCTGCTGATTCCTACCAGATCGGGGCCATTGCAGGCATCATCA
  TTCTTGTCCTAGTTGTTCTCTTCCTACTGGCATTGTTCATTATTTATAGACACAAGCAGAAGGGAAAGGA
  ATCAAGCATGCCAGCAGTTACCTACACCCCTGCTATGAGGGTCGTCAATGCAGATTATACCATTTCAGGA
  ACCCTTCCTCACAGCAATGGTGGAAACGCTAATAGCCACTACTTCACCAATCCCAGTTACCACACGCTCA
  CCCAGTGTGCCACATCCCCTCACGTCAACAACAGGGACAGGATGACTGTCACGAAGTCAAAAAACAATCA
  ACTGTTTGTGAATCTTAAAAATGTGAACCCTGGGAAGAGAGGCCCTGTGGGGGACTGCACTGGGACATTG
  CCGGCTGACTGGAAACATGGCGGCTACCTCAACGAGCTCGGTGCTTTTGGACTTGACAGAAGCTATATGG
  GAAAATCCTTAAAAGACCTGGGAAAGAATTCTGAATATAATTCAAGTAACTGCTCCCTAAGCAGTTCTGA
  GAACCCATATGCCACTATTAAAGACCCACCTGTACTTATCCCGAAAAGCTCAGAGTGTGGTTATGTGGAG
  ATGAAATCGCCGGCACGAAGAGATTCCCCATATGCAGAGATCAATAACTCAACTTCAGCCAACAGGAATG
  TCTATGAAGTTGAACCTACAGTGAGTGTTGTCCAAGGAGTATTCAGCAATAATGGGCGTCTCTCCCAGGA
  TCCATATGACCTCCCAAAGAACAGTCACATCCCTTGTCATTATGACCTGCTGCCAGTCCGAGACAGTTCA
  TCCTCCCCTAAGCAAGAGGACAGTGGAGGTAGCAGCAGCAACAGCAGCAGCAGCAGTGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217883 representing NM_032446
  Red=Cloning site Green=Tags(s)
  
  MVISLNSCLSFICLLLCHWIGTASPLNLEDPNVCSHWESYSVTVQESYPHPFDQIYYTSCTDILNWFKCT
  RHRVSYRTAYRHGEKTMYRRKSQCCPGFYESGEMCVPHCADKCVHGRCIAPNTCQCEPGWGGTNCSSACD
  GDHWGPHCTSRCQCKNGALCNPITGACHCAAGFRGWRCEDRCEQGTYGNDCHQRCQCQNGATCDHVTGEC
  RCPPGYTGAFCEDLCPPGKHGPQCEQRCPCQNGGVCHHVTGECSCPSGWMGTVCGQPCPEGRFGKNCSQE
  CQCHNGGTCDAATGQCHCSPGYTGERCQDECPVGTYGVLCAETCQCVNGGKCYHVSGACLCEAGFAGERC
  EARLCPEGLYGIKCDKRCPCHLENTHSCHPMSGECACKPGWSGLYCNETCSPGFYGEACQQICSCQNGAD
  CDSVTGKCTCAPGFKGIDCSTPCPLGTYGINCSSRCGCKNDAVCSPVDGSCTCKAGWHGVDCSIRCPSGT
  WGFGCNLTCQCLNGGACNTLDGTCTCAPGWRGEKCELPCQDGTYGLNCAERCDCSHADGCHPTTGHCRCL
  PGWSGVHCDSVCAEGRWGPNCSLPCYCKNGASCSPDDGICECAPGFRGTTCQRICSPGFYGHRCSQTCPQ
  CVHSSGPCHHITGLCDCLPGFTGALCNEVCPSGRFGKNCAGICTCTNNGTCNPIDRSCQCYPGWIGSDCS
  QPCPPAHWGPNCIHTCNCHNGAFCSAYDGECKCTPGWTGLYCTQRCPLGFYGKDCALICQCQNGADCDHI
  SGQCTCRTGFMGRHCEQKCPSGTYGYGCRQICDCLNNSTCDHITGTCYCSPGWKGARCDQAGVIIVGNLN
  SLSRTSTALPADSYQIGAIAGIIILVLVVLFLLALFIIYRHKQKGKESSMPAVTYTPAMRVVNADYTISG
  TLPHSNGGNANSHYFTNPSYHTLTQCATSPHVNNRDRMTVTKSKNNQLFVNLKNVNPGKRGPVGDCTGTL
  PADWKHGGYLNELGAFGLDRSYMGKSLKDLGKNSEYNSSNCSLSSSENPYATIKDPPVLIPKSSECGYVE
  MKSPARRDSPYAEINNSTSANRNVYEVEPTVSVVQGVFSNNGRLSQDPYDLPKNSHIPCHYDLLPVRDSS
  SSPKQEDSGGSSSNSSSSSE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032446
• ORF Size: 3420 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_032446.1, NP_115822.1
• RefSeq Size: 7522
• RefSeq ORF: 3423
• Locus ID: 84466
• Domains: EGF_Lam, EGF, EGF
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a member of the multiple epidermal growth factor-like domains protein family. The encoded protein plays a role in cell adhesion, motility and proliferation, and is a critical mediator of apoptotic cell phagocytosis as well as amyloid-beta peptide uptake in the brain. Expression of this gene may be associated with schizophrenia, and mutations in this gene are a cause of early-onset myopathy, areflexia, respiratory distress, and dysphagia (EMARDD) as well as congenital myopathy with minicores. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Apr 2012]