AAMP (NM_001087) Human Tagged ORF Clone

CAT#: RG218474

AAMP (GFP-tagged) - Human angio-associated, migratory cell protein (AAMP)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AAMP
• Synonyms: angio-associated, migratory cell protein
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG218474 representing NM_001087
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCCGAATCGGAAAGCGGGGCTGCTGCTGACACCCCCCCACTGGAGACCCTAAGCTTCCATGGTG
  ATGAAGAGATTATCGAGGTGGTAGAACTTGATCCCGGTCCGCCGGACCCAGATGACCTGGCCCAGGAGAT
  GGAAGATGTGGACTTTGAGGAAGAAGAGGAGGAAGAGGGCAACGAAGAGGGCTGGGTTCTAGAACCCCAG
  GAAGGGGTGGTCGGCAGCATGGAGGGCCCCGACGATAGCGAGGTCACCTTTGCATTGCACTCAGCATCTG
  TGTTTTGTGTGAGCCTGGACCCCAAGACCAATACCTTGGCAGTGACCGGGGGTGAAGATGACAAAGCCTT
  CGTATGGCGGCTCAGCGATGGGGAGCTGCTCTTTGAGTGTGCAGGCCATAAAGACTCTGTGACTTGTGCT
  GGTTTCAGCCATGACTCCACTCTAGTGGCCACAGGGGACATGAGTGGCCTCTTGAAAGTGTGGCAGGTGG
  ACACTAAGGAGGAGGTCTGGTCCTTTGAAGCGGGAGACCTGGAGTGGATGGAGTGGCATCCTCGGGCACC
  TGTCCTGTTGGCGGGCACAGCTGACGGCAACACCTGGATGTGGAAAGTCCCGAATGGTGACTGCAAGACC
  TTCCAGGGTCCCAACTGCCCAGCCACCTGTGGCCGAGTCCTCCCTGATGGGAAGAGAGCTGTGGTAGGCT
  ATGAAGATGGGACCATCAGGATTTGGGACCTGAAGCAGGGAAGCCCTATCCATGTACTGAAAGGGACTGA
  GGGTCACCAGGGCCCACTCACCTGTGTTGCTGCCAACCAGGATGGCAGCTTGATCCTAACTGGCTCTGTG
  GACTGCCAGGCCAAGCTGGTCAGTGCCACCACCGGCAAGGTGGTGGGTGTTTTTAGACCTGAGACTGTGG
  CCTCCCAGCCCAGCCTGGGAGAAGGGGAGGAGAGTGAGTCCAACTCGGTGGAGTCCTTGGGCTTCTGCAG
  TGTGATGCCCCTGGCAGCTGTTGGCTACCTGGATGGGACCTTGGCCATCTATGACCTGGCTACGCAGACT
  CTTAGGCATCAGTGTCAGCACCAGTCGGGCATCGTGCAGCTGCTGTGGGAGGCAGGCACTGCCGTGGTAT
  ATACCTGCAGCCTGGATGGCATCGTGCGCCTCTGGGACGCCCGGACCGGCCGCCTGCTTACTGACTACCG
  GGGCCACACGGCTGAGATCCTGGACTTTGCCCTCAGCAAAGATGCCTCCCTGGTGGTGACCACGTCAGGA
  GACCACAAAGCGAAAGTATTTTGTGTCCAAAGGCCTGACCGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG218474 representing NM_001087
  Red=Cloning site Green=Tags(s)
  
  MESESESGAAADTPPLETLSFHGDEEIIEVVELDPGPPDPDDLAQEMEDVDFEEEEEEEGNEEGWVLEPQ
  EGVVGSMEGPDDSEVTFALHSASVFCVSLDPKTNTLAVTGGEDDKAFVWRLSDGELLFECAGHKDSVTCA
  GFSHDSTLVATGDMSGLLKVWQVDTKEEVWSFEAGDLEWMEWHPRAPVLLAGTADGNTWMWKVPNGDCKT
  FQGPNCPATCGRVLPDGKRAVVGYEDGTIRIWDLKQGSPIHVLKGTEGHQGPLTCVAANQDGSLILTGSV
  DCQAKLVSATTGKVVGVFRPETVASQPSLGEGEESESNSVESLGFCSVMPLAAVGYLDGTLAIYDLATQT
  LRHQCQHQSGIVQLLWEAGTAVVYTCSLDGIVRLWDARTGRLLTDYRGHTAEILDFALSKDASLVVTTSG
  DHKAKVFCVQRPDR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001087
• ORF Size: 1302 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001087.2, NP_001078.2
• RefSeq Size: 1792 bp
• RefSeq ORF: 1305 bp
• Locus ID: 14
• Cytogenetics: 2q35
• Domains: WD40
• Gene Summary: 'The gene is a member of the immunoglobulin superfamily. The encoded protein is associated with angiogenesis, with potential roles in endothelial tube formation and the migration of endothelial cells. It may also regulate smooth muscle cell migration via the RhoA pathway. The encoded protein can bind to heparin and may mediate heparin-sensitive cell adhesion. [provided by RefSeq, Oct 2014]'