Myosin Heavy chain 1 (MYH1) (NM_005963) Human Tagged ORF Clone

CAT#: RG218544

MYH1 (GFP-tagged) - Human myosin, heavy chain 1, skeletal muscle, adult (MYH1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MYH1
• Synonyms: HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG218544 representing NM_005963
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTCTGACTCTGAGATGGCCATTTTTGGGGAGGCTGCTCCTTTCCTCCGAAAGTCTGAAAGGGAGC
  GAATTGAAGCCCAGAACAAGCCTTTTGATGCCAAGACATCAGTCTTTGTGGTGGACCCTAAGGAGTCCTT
  TGTGAAAGCAACAGTGCAGAGCAGGGAAGGGGGGAAGGTGACAGCTAAGACCGAAGCTGGAGCTACTGTA
  ACAGTGAAAGATGACCAAGTCTTCCCCATGAACCCTCCCAAATATGACAAGATCGAGGACATGGCCATGA
  TGACTCATCTACACGAGCCTGCTGTGCTGTACAACCTCAAAGAGCGCTACGCAGCCTGGATGATCTACAC
  CTACTCAGGCTTGTTCTGTGTCACTGTCAACCCCTACAAGTGGTTGCCAGTGTATAATGCAGAAGTGGTG
  ACAGCCTACCGAGGCAAAAAGCGCCAGGAAGCCCCACCCCACATCTTCTCCATCTCTGACAATGCCTATC
  AGTTCATGCTGACTGATCGGGAGAATCAGTCTATCTTGATCACCGGAGAATCTGGCGCAGGGAAGACTGT
  GAACACCAAGCGTGTCATCCAGTACTTTGCAACAATTGCAGTTACTGGGGAGAAGAAGAAGGAAGAAGTT
  ACTTCTGGCAAAATGCAGGGGACTCTGGAAGATCAAATCATCAGTGCCAACCCCCTACTGGAGGCCTTTG
  GCAACGCCAAGACCGTGAGGAATGACAACTCCTCTCGCTTTGGTAAATTCATCAGGATCCACTTCGGTAC
  CACAGGGAAACTGGCTTCTGCTGATATTGAAACATATCTTCTGGAGAAGTCTAGAGTTACTTTCCAGCTA
  AAGGCTGAAAGAAGCTATCATATTTTTTATCAGATCATGTCTAACAAGAAGCCAGATCTAATTGAAATGC
  TCCTGATCACCACCAACCCATACGATTATGCCTTCGTCAGTCAAGGGGAGATCACAGTGCCCAGCATTGA
  TGACCAAGAAGAGTTGATGGCTACAGATAGTGCCATTGAAATTCTGGGCTTTACTTCAGATGAAAGAGTG
  TCCATCTATAAGCTCACAGGGGCTGTGATGCATTATGGGAACATGAAATTCAAGCAAAAGCAGCGTGAGG
  AGCAAGCTGAGCCAGATGGCACTGAAGTTGCTGACAAGGCAGCCTATCTCCAAAATCTGAACTCTGCAGA
  TCTGCTCAAAGCCCTCTGCTACCCTAGGGTCAAGGTCGGCAATGAGTATGTCACCAAAGGTCAAACTGTG
  CAGCAGGTGTACAATGCAGTGGGTGCTCTGGCCAAAGCTGTCTACGATAAGATGTTCTTGTGGATGGTCA
  CCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCAGGCAGTACTTCATTGGGGTCTTGGACATTGCTGG
  CTTTGAGATCTTTGATTTCAACAGCCTGGAGCAGCTGTGCATCAACTTCACCAATGAGAAACTGCAACAG
  TTTTTCAACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAAGGCATTGAGTGGACGTTCA
  TTGACTTTGGGATGGACCTGGCTGCCTGCATCGAGCTCATCGAGAAGCCTATGGGCATCTTCTCCATCCT
  GGAAGAGGAGTGCATGTTCCCCAAGGCGACAGACACCTCCTTCAAGAACAAGCTGTATGAACAACATCTT
  GGAAAATCCAATAACTTCCAGAAGCCCAAGCCTGCCAAAGGCAAGCCTGAGGCCCACTTCTCTTTGATTC
  ACTATGCTGGCACCGTGGACTACAACATTGCCGGCTGGCTTGACAAGAACAAGGACCCCCTGAATGAGAC
  TGTGGTGGGGCTGTACCAGAAGTCTGCAATGAAGACTCTGGCTCTCCTCTTTGTTGGGGCAACGGGAGCG
  GAAGCAGAGGCTGGCGGTGGAAAGAAAGGTGGTAAGAAGAAGGGTTCTTCTTTCCAGACTGTGTCGGCTC
  TCTTCAGGGAGAATTTGAATAAGCTGATGACCAACTTGAGGAGCACTCACCCCCACTTTGTGCGGTGCAT
  CATCCCCAATGAAACTAAAACTCCTGGTGCCATGGAGCATGAGCTTGTCCTGCATCAGCTGAGGTGTAAC
  GGTGTGCTGGAAGGCATCCGCATCTGCAGGAAAGGCTTCCCAAGCAGAATCCTTTATGCAGACTTCAAAC
  AGAGATACAAGGTGTTAAATGCAAGTGCTATCCCTGAAGGACAATTCATCGATAGCAAGAAGGCTTCAGA
  GAAGCTCCTGGGGTCCATTGACATTGACCACACCCAGTATAAATTTGGTCACACCAAGGTCTTTTTCAAA
  GCTGGTCTTCTGGGGCTCCTAGAGGAGATGCGAGATGAGAAGCTGGCCCAGCTGATTACCCGAACCCAGG
  CCATGTGCAGAGGGTTCTTGGCAAGAGTGGAGTACCAGAAAATGGTGGAAAGAAGAGAGTCCATCTTCTG
  CATCCAGTACAATGTCCGTGCCTTCATGAATGTCAAGCACTGGCCCTGGATGAAGCTGTATTTCAAGATC
  AAACCCCTCCTCAAAAGTGCAGAGACAGAGAAGGAGATGGCCAACATGAAGGAAGAATTTGAGAAAACCA
  AAGAAGAGCTGGCTAAGACCGAGGCAAAAAGGAAAGAGCTGGAAGAAAAAATGGTGACTCTGATGCAAGA
  AAAAAATGACTTGCAACTCCAGGTTCAAGCTGAAGCTGACAGCTTGGCTGATGCAGAGGAAAGGTGTGAC
  CAGCTAATCAAAACCAAAATCCAGCTAGAAGCCAAAATCAAAGAGGTGACTGAGAGAGCTGAGGATGAGG
  AAGAGATCAATGCTGAGCTGACAGCCAAGAAGAGGAAACTGGAGGATGAATGTTCAGAACTCAAGAAAGA
  CATTGATGACCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAACATGCCACAGAAAACAAGGTGAAA
  AACCTCACAGAAGAGATGGCGGGTCTGGATGAAACCATTGCTAAGCTGACCAAGGAGAAGAAGGCTCTCC
  AGGAGGCCCACCAGCAGACCCTGGATGACCTGCAGGCAGAGGAGGACAAAGTCAACACCCTGACCAAAGC
  TAAAATCAAACTTGAACAACAAGTGGATGATCTTGAAGGATCTTTGGAACAAGAAAAGAAAATCCGGATG
  GATCTAGAAAGAGCAAAGAGAAAACTAGAGGGAGACCTAAAATTGGCTCAAGAATCCGCAATGGATATAG
  AAAATGACAAACAACAACTTGATGAAAAGCTTAAAAAGAAAGAGTTTGAAATGAGCGGTCTGCAAAGCAA
  GATTGAAGATGAACAAGCCCTTGGTATGCAGCTGCAGAAGAAAATCAAGGAGTTACAAGCCCGCATTGAG
  GAGCTGGAGGAGGAAATCGAGGCAGAGCGGGCCTCCCGGGCCAAAGCAGAGAAGCAGCGCTCTGATCTCT
  CCCGGGAGCTGGAGGAGATCAGTGAGAGGCTGGAAGAAGCCGGTGGGGCCACCTCGGCCCAGATTGAGAT
  GAACAAGAAGCGGGAAGCTGAGTTCCAGAAAATGCGCAGGGACCTGGAGGAGGCCACCCTACAGCATGAG
  GCCACGGCGGCCACCCTGAGGAAGAAGCATGCAGATAGTGTGGCCGAGCTTGGGGAGCAGATTGACAACC
  TGCAGCGAGTGAAGCAGAAGCTGGAGAAGGAGAAGAGTGAGATGAAGATGGAGATCGATGACCTTGCTAG
  TAACATGGAGACTGTCTCCAAAGCCAAGGGAAACCTTGAAAAGATGTGCCGCGCTCTAGAAGATCAACTG
  AGTGAAATTAAGACCAAGGAAGAGGAGCAGCAGCGGCTGATCAATGACCTCACAGCACAGAGAGCGCGCC
  TGCAAACAGAATCAGGTGAATATTCACGCCAGCTAGATGAAAAGGACACACTAGTTTCACAGCTCTCGAG
  GGGCAAACAAGCCTTTACTCAACAGATTGAGGAACTGAAAAGGCAACTTGAAGAGGAGATAAAGGCCAAG
  AGTGCCCTGGCACATGCCCTGCAGTCCTCCCGCCATGACTGTGACCTGCTGCGGGAACAGTATGAGGAGG
  AGCAGGAAGCCAAGGCCGAGCTACAGAGAGCAATGTCCAAGGCCAACAGTGAGGTTGCCCAGTGGAGGAC
  CAAATATGAGACAGATGCCATCCAGCGCACAGAGGAGCTGGAGGAGGCCAAGAAGAAGCTGGCTCAGCGT
  CTGCAGGATGCTGAGGAACATGTAGAAGCTGTGAATGCCAAATGTGCTTCCCTTGAGAAGACGAAGCAGA
  GGCTCCAGAATGAAGTTGAGGACCTCATGATTGATGTTGAGAGGACAAATGCTGCCTGTGCCGCCCTGGA
  CAAAAAGCAAAGGAACTTTGATAAGATCCTGGCAGAATGGAAACAGAAGTGTGAAGAAACTCATGCTGAA
  CTTGAAGCTTCTCAAAAGGAATCCCGCTCACTCAGCACAGAACTATTTAAGATTAAGAATGCTTATGAGG
  AATCTTTAGACCAACTTGAAACCTTGAAACGGGAAAATAAGAATCTGCAGCAGGAGATTTCTGATCTCAC
  TGAACAGATTGCAGAAGGAGGAAAGCGCATCCATGAACTGGAAAAAATAAAGAAGCAAGTTGAGCAAGAA
  AAGTCTGAACTTCAGGCTGCCTTAGAGGAGGCAGAGGCATCTCTTGAACATGAAGAGGGAAAGATCCTGC
  GCATCCAGCTTGAGTTGAACCAAGTCAAGTCTGAGGTTGATAGGAAAATTGCTGAAAAAGATGAGGAAAT
  TGACCAGATGAAGAGAAACCACATTAGAATCGTGGAGTCCATGCAGAGCACACTGGATGCTGAGATCAGG
  AGCAGGAATGATGCCATTAGGCTCAAGAAGAAGATGGAGGGAGACCTCAATGAAATGGAAATCCAGCTGA
  ACCATGCCAACCGCATGGCTGCTGAGGCCCTGAGGAACTATAGGAACACCCAAGCCATCCTCAAGGATAC
  CCAGCTCCACCTAGATGATGCTCTCCGGAGCCAAGAGGACCTGAAGGAACAGCTGGCTATGGTGGAGCGC
  AGAGCCAACCTGCTGCAGGCTGAGATCGAGGAACTACGAGCCACTCTGGAACAGACGGAGAGGAGCAGGA
  AAATCGCAGAACAGGAGCTCCTGGATGCCAGTGAACGTGTTCAGCTCCTGCACACCCAGAACACCAGCCT
  GATCAACACCAAGAAGAAGCTGGAGACAGACATTTCCCAAATCCAGGGAGAGATGGAAGACATCATCCAG
  GAAGCCCGCAATGCAGAAGAGAAGGCCAAGAAGGCCATCACTGATGCTGCCATGATGGCTGAGGAGCTGA
  AGAAGGAACAGGACACCAGCGCCCATCTGGAGCGGATGAAGAAGAACTTGGAACAGACGGTGAAGGACCT
  GCAGCATCGTCTGGATGAGGCTGAGCAGCTGGCCCTGAAGGGTGGGAAGAAGCAGATCCAGAAACTGGAG
  GCCAGGGTTCGTGAACTTGAAGGTGAAGTTGAAAGTGAACAGAAGCGCAATGTTGAAGCTGTCAAGGGTC
  TACGCAAACATGAGAGAAAAGTGAAGGAACTCACTTACCAAACTGAGGAAGACCGCAAGAATATTCTCAG
  GCTGCAGGACCTGGTGGACAAGCTGCAAGCAAAGGTGAAATCCTACAAGAGACAAGCTGAAGAAGCGGAG
  GAACAATCCAACGTCAACCTCTCCAAATTCCGGAGGATCCAGCACGAGCTGGAGGAGGCCGAGGAAAGGG
  CTGACATTGCTGAGTCCCAGGTCAACAAGCTGAGGGTGAAGAGCAGGGAGGTTCACACAAAAATCATAAG
  TGAAGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG218544 representing NM_005963
  Red=Cloning site Green=Tags(s)
  
  MSSDSEMAIFGEAAPFLRKSERERIEAQNKPFDAKTSVFVVDPKESFVKATVQSREGGKVTAKTEAGATV
  TVKDDQVFPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVV
  TAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEV
  TSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQL
  KAERSYHIFYQIMSNKKPDLIEMLLITTNPYDYAFVSQGEITVPSIDDQEELMATDSAIEILGFTSDERV
  SIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLQNLNSADLLKALCYPRVKVGNEYVTKGQTV
  QQVYNAVGALAKAVYDKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQ
  FFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHL
  GKSNNFQKPKPAKGKPEAHFSLIHYAGTVDYNIAGWLDKNKDPLNETVVGLYQKSAMKTLALLFVGATGA
  EAEAGGGKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCN
  GVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIDIDHTQYKFGHTKVFFK
  AGLLGLLEEMRDEKLAQLITRTQAMCRGFLARVEYQKMVERRESIFCIQYNVRAFMNVKHWPWMKLYFKI
  KPLLKSAETEKEMANMKEEFEKTKEELAKTEAKRKELEEKMVTLMQEKNDLQLQVQAEADSLADAEERCD
  QLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVK
  NLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKIKLEQQVDDLEGSLEQEKKIRM
  DLERAKRKLEGDLKLAQESAMDIENDKQQLDEKLKKKEFEMSGLQSKIEDEQALGMQLQKKIKELQARIE
  ELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHE
  ATAATLRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNMETVSKAKGNLEKMCRALEDQL
  SEIKTKEEEQQRLINDLTAQRARLQTESGEYSRQLDEKDTLVSQLSRGKQAFTQQIEELKRQLEEEIKAK
  SALAHALQSSRHDCDLLREQYEEEQEAKAELQRAMSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQR
  LQDAEEHVEAVNAKCASLEKTKQRLQNEVEDLMIDVERTNAACAALDKKQRNFDKILAEWKQKCEETHAE
  LEASQKESRSLSTELFKIKNAYEESLDQLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKIKKQVEQE
  KSELQAALEEAEASLEHEEGKILRIQLELNQVKSEVDRKIAEKDEEIDQMKRNHIRIVESMQSTLDAEIR
  SRNDAIRLKKKMEGDLNEMEIQLNHANRMAAEALRNYRNTQAILKDTQLHLDDALRSQEDLKEQLAMVER
  RANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMEDIIQ
  EARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLE
  ARVRELEGEVESEQKRNVEAVKGLRKHERKVKELTYQTEEDRKNILRLQDLVDKLQAKVKSYKRQAEEAE
  EQSNVNLSKFRRIQHELEEAEERADIAESQVNKLRVKSREVHTKIISEE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005963
• ORF Size: 5817 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005963.2, NP_005954.2
• RefSeq Size: 5925 bp
• RefSeq ORF: 5820 bp
• Locus ID: 4619
• Cytogenetics: 17p13.1
• Protein Pathways: Tight junction, Viral myocarditis
• Gene Summary: 'Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development. [provided by RefSeq, Jul 2008]'