Myosin Heavy chain 1 (MYH1) (NM_005963) Human Untagged Clone

CAT#: SC303712

MYH1 (untagged)-Human myosin, heavy chain 1, skeletal muscle, adult (MYH1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MYH1
• Synonyms: HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005963, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGTTCCGACTCTGAGATGGCCATTTTTGGGGAGGCTGCTCCTTTCCTCCGAAAGTCTGAAAGGGAGC
  GAATTGAAGCCCAGAACAAGCCTTTTGATGCCAAGACATCAGTCTTTGTGGTGGACCCTAAGGAGTCCTT
  TGTGAAAGCAACAGTGCAGAGCAGGGAAGGGGGGAAGGTGACAGCTAAGACCGAAGCTGGAGCTACTGTA
  ACAGTGAAAGATGACCAAGTCTTCCCCATGAACCCTCCCAAATATGACAAGATCGAGGACATGGCCATGA
  TGACTCATCTACACGAGCCTGCTGTGCTGTACAACCTCAAAGAGCGCTACGCAGCCTGGATGATCTACAC
  CTACTCAGGCTTGTTCTGTGTCACTGTCAACCCCTACAAGTGGTTGCCAGTGTATAATGCAGAGGTGGTG
  ACAGCCTACCGAGGCAAAAAGCGCCAGGAGGCCCCACCCCACATCTTCTCCATCTCTGACAATGCCTATC
  AGTTCATGCTGACTGATCGGGAGAATCAGTCTATCTTGATCACCGGAGAATCTGGCGCAGGGAAGACTGT
  GAACACCAAGCGTGTCATCCAGTACTTTGCAACAATTGCAGTTACTGGGGAGAAGAAGAAGGAAGAAGTT
  ACTTCTGGCAAAATGCAGGGGACTCTGGAAGATCAAATCATCAGTGCCAACCCCCTACTGGAGGCCTTTG
  GCAACGCCAAGACCGTGAGGAATGACAACTCCTCTCGCTTTGGTAAATTCATCAGGATCCACTTCGGTAC
  CACAGGGAAACTGGCTTCTGCTGATATTGAAACATATCTTCTGGAGAAGTCTAGAGTTACTTTCCAGCTA
  AAGGCTGAAAGAAGCTATCATATTTTTTATCAGATCATGTCTAACAAGAAGCCAGATCTAATTGAAATGC
  TCCTGATCACCACCAACCCATACGATTATGCCTTCGTCAGTCAAGGGGAGATCACAGTGCCCAGCATTGA
  TGACCAAGAAGAGTTGATGGCTACAGATAGTGCCATTGAAATTCTGGGCTTTACTTCAGATGAAAGAGTG
  TCCATCTATAAGCTCACAGGGGCTGTGATGCATTATGGGAACATGAAATTCAAGCAAAAGCAGCGTGAGG
  AGCAAGCTGAGCCAGATGGCACTGAAGTTGCTGACAAGGCAGCCTATCTCCAAAATCTGAACTCTGCAGA
  TCTGCTCAAAGCCCTCTGCTACCCTAGGGTCAAGGTCGGCAATGAGTATGTCACCAAAGGTCAAACTGTG
  CAGCAGGTGTACAATGCAGTGGGTGCTCTGGCCAAAGCTGTCTACGATAAGATGTTCTTGTGGATGGTCA
  CCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCAGGCAGTACTTCATTGGGGTCTTGGACATTGCTGG
  CTTTGAGATCTTTGATTTCAACAGCCTGGAGCAGCTGTGCATCAACTTCACCAATGAGAAACTGCAACAG
  TTTTTCAACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAAGGCATTGAGTGGACGTTCA
  TTGACTTTGGGATGGACCTGGCTGCCTGCATCGAGCTCATCGAGAAGCCTATGGGCATCTTCTCCATCCT
  GGAAGAGGAGTGCATGTTCCCCAAGGCGACAGACACCTCCTTCAAGAACAAGCTGTATGAACAACATCTT
  GGAAAATCCAATAACTTCCAGAAGCCCAAGCCTGCCAAAGGCAAGCCTGAGGCCCACTTCTCTTTGATTC
  ACTATGCTGGCACCGTGGACTACAACATTGCCGGCTGGCTTGACAAGAACAAGGACCCCCTGAATGAGAC
  TGTGGTGGGGCTGTACCAGAAGTCTGCAATGAAGACTCTGGCTCTCCTCTTTGTTGGGGCAACGGGAGCG
  GAAGCAGAGGCTGGCGGTGGAAAGAAAGGTGGTAAGAAGAAGGGTTCTTCTTTCCAGACTGTGTCTGCTC
  TCTTCAGGGAGAATTTGAATAAGCTGATGACCAACTTGAGGAGCACTCACCCCCACTTTGTGCGGTGCAT
  CATCCCCAATGAAACTAAAACTCCTGGTGCCATGGAGCATGAGCTTGTCCTGCATCAGCTGAGGTGTAAC
  GGTGTGCTGGAAGGCATCCGCATCTGCAGGAAAGGCTTCCCAAGCAGAATCCTTTATGCAGACTTCAAAC
  AGAGATACAAGGTGTTAAATGCAAGTGCTATCCCTGAAGGACAATTCATCGATAGCAAGAAGGCTTCAGA
  GAAGCTCCTGGGGTCCATTGACATTGACCACACCCAGTATAAATTTGGTCACACCAAGGTCTTTTTCAAA
  GCTGGTCTTCTGGGGCTCCTAGAGGAGATGCGAGATGAGAAGCTGGCCCAGCTGATTACCCGAACCCAGG
  CCATGTGCAGAGGGTTCTTGGCAAGAGTGGAGTACCAGAAAATGGTGGAAAGAAGAGAGTCCATCTTCTG
  CATCCAGTACAATGTCCGTGCCTTCATGAATGTGAAGCACTGGCCCTGGATGAAGCTGTATTTCAAGATC
  AAACCCCTCCTCAAAAGTGCAGAGACAGAGAAGGAGATGGCCAACATGAAGGAAGAATTTGAGAAAACCA
  AAGAAGAGCTGGCTAAGACCGAGGCAAAAAGGAAAGAGCTGGAAGAAAAAATGGTTACTCTGATGCAAGA
  AAAAAATGACTTGCAACTCCAGGTTCAAGCTGAAGCTGACAGCTTGGCTGATGCAGAGGAAAGGTGTGAC
  CAGCTAATCAAAACCAAAATCCAGCTAGAAGCCAAAATCAAAGAGGTGACTGAGAGAGCTGAGGATGAGG
  AAGAGATCAATGCTGAGCTGACAGCCAAGAAGAGGAAACTGGAGGATGAATGTTCAGAACTCAAGAAAGA
  CATTGATGACCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAACATGCCACAGAAAACAAGGTGAAA
  AACCTCACAGAAGAGATGGCGGGTCTGGATGAAACCATTGCTAAGCTGACCAAGGAGAAGAAGGCTCTCC
  AGGAGGCCCACCAGCAGACCCTGGATGACCTGCAGGCAGAGGAGGACAAAGTCAACACCCTGACCAAAGC
  TAAAATCAAACTTGAACAACAAGTGGATGATCTTGAAGGATCTTTGGAACAAGAAAAGAAAATCCGGATG
  GATCTAGAAAGAGCAAAGAGAAAACTAGAGGGAGACCTAAAATTGGCTCAAGAATCCACAATGGATATAG
  AAAATGACAAACAACAACTTGATGAAAAGCTTAAAAAGAAAGAGTTTGAAATGAGCGGTCTGCAAAGCAA
  GATTGAAGATGAACAAGCCCTTGGTATGCAGCTGCAGAAGAAAATCAAGGAGTTACAAGCCCGCATTGAG
  GAGCTGGAGGAGGAAATCGAGGCAGAGCGGGCCTCCCGGGCCAAAGCAGAGAAGCAGCGCTCTGATCTCT
  CCCGGGAGCTGGAGGAGATCAGCGAGAGGCTGGAAGAAGCCGGTGGGGCCACCTCAGCCCAGATTGAGAT
  GAACAAGAAGCGGGAGGCTGAGTTCCAGAAAATGCGCAGGGACCTGGAGGAGGCCACCCTACAGCATGAA
  GCCACGGCGGCCACCCTGAGGAAGAAGCATGCAGATAGTGTGGCCGAGCTTGGGGAGCAGATTGACAACC
  TGCAGCGAGTGAAGCAGAAGCTGGAGAAGGAGAAGAGTGAGATGAAGATGGAGATCGATGACCTTGCTAG
  TAACATGGAGACTGTCTCCAAAGCCAAGGGAAACCTTGAAAAGATGTGCCGCGCTCTAGAAGATCAACTG
  AGTGAAATTAAGACCAAGGAAGAGGAGCAGCAGCGGCTGATCAATGACCTCACAGCACAGAGAGCGCGCC
  TGCAAACAGAATCAGGTGAATATTCACGCCAGCTAGATGAAAAGGACACACTAGTTTCACAGCTCTCGAG
  GGGCAAACAAGCCTTTACACAACAGATTGAGGAACTGAAAAGGCAACTTGAAGAGGAGATAAAGGCCAAG
  AGTGCCCTGGCACATGCCCTGCAGTCCTCCCGCCATGACTGTGACCTGCTGCGGGAACAGTATGAGGAGG
  AGCAGGAAGCCAAGGCCGAGCTACAGAGAGCAATGTCCAAGGCCAACAGTGAGGTTGCCCAGTGGAGGAC
  CAAATATGAGACAGATGCCATCCAGCGCACAGAGGAGCTGGAGGAGGCCAAGAAGAAGCTGGCTCAGCGT
  CTGCAGGATGCTGAGGAACATGTAGAAGCTGTGAATGCCAAATGTGCTTCCCTTGAGAAGACGAAGCAGA
  GGCTCCAGAATGAAGTTGAGGACCTCATGATTGATGTTGAGAGGACAAATGCTGCCTGTGCCGCCCTGGA
  CAAAAAGCAAAGGAACTTTGATAAGATCCTGGCAGAATGGAAACAGAAGTGTGAAGAAACTCATGCTGAA
  CTTGAAGCTTCTCAAAAGGAATCCCGCTCACTCAGCACAGAACTATTTAAGATTAAGAATGCTTATGAGG
  AATCTTTAGACCAACTTGAAACCTTGAAACGGGAAAATAAGAATTTGCAACAGGAGATTTCTGATCTCAC
  TGAACAGATTGCAGAAGGAGGAAAGCGCATCCATGAACTGGAAAAAATAAAGAAGCAAGTTGAGCAAGAA
  AAGTCTGAACTTCAGGCTGCCTTAGAGGAGGCAGAGGCATCTCTTGAACATGAAGAGGGAAAGATCCTGC
  GCATCCAGCTTGAGTTGAACCAAGTCAAGTCTGAGGTTGATAGGAAAATTGCTGAAAAAGATGAGGAAAT
  TGACCAGATGAAGAGAAACCACATTAGAATCGTGGAGTCCATGCAGAGCACACTGGATGCTGAGATCAGG
  AGCAGGAATGATGCCATTAGGCTCAAGAAGAAGATGGAGGGAGACCTCAATGAAATGGAAATCCAGCTGA
  ACCATGCCAACCGCATGGCTGCTGAGGCCCTGAGGAACTATAGGAACACCCAAGCCATCCTCAAGGATAC
  CCAGCTCCACCTGGATGATGCTCTCCGGAGCCAGGAGGACCTGAAGGAACAGCTGGCTATGGTGGAGCGC
  AGAGCCAACCTGCTGCAGGCTGAGATCGAGGAGCTGCGGGCCACTCTGGAACAGACAGAGAGGAGCAGGA
  AAATCGCAGAACAGGAGCTCCTGGATGCCAGTGAGCGTGTTCAGCTCCTGCACACCCAGAACACCAGCCT
  GATCAACACCAAGAAGAAGCTGGAGACAGACATTTCCCAAATCCAGGGAGAGATGGAAGACATCATCCAG
  GAAGCCCGCAATGCAGAAGAGAAGGCCAAGAAGGCCATCACTGATGCTGCCATGATGGCTGAGGAGCTGA
  AGAAGGAACAGGACACCAGCGCCCATCTGGAGCGGATGAAGAAGAACCTGGAACAGACGGTGAAGGACCT
  GCAGCATCGTCTGGATGAGGCTGAGCAGCTGGCCCTGAAGGGTGGGAAGAAGCAGATCCAGAAACTGGAG
  GCCAGGGTTCGTGAACTTGAAGGTGAAGTTGAAAGTGAACAGAAGCGCAATGTTGAAGCTGTCAAGGGTC
  TACGCAAACATGAGAGAAAAGTGAAGGAACTCACTTACCAAACTGAGGAAGACCGCAAGAATATTCTCAG
  GCTCCAGGACCTGGTGGACAAACTGCAAGCAAAGGTGAAATCCTACAAGAGACAAGCTGAAGAAGCGGAG
  GAACAATCCAACGTCAACCTCTCCAAATTCCGCAGGATCCAGCACGAGCTGGAGGAGGCCGAGGAACGGG
  CTGACATTGCTGAGTCCCAGGTCAACAAGCTGAGGGTGAAGAGCAGGGAGGTTCACACAAAAATCATAAG
  TGAAGAGTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_005963
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005963.3, NP_005954.3
• RefSeq Size: 6038 bp
• RefSeq ORF: 5820 bp
• Locus ID: 4619
• Cytogenetics: 17p13.1
• Protein Pathways: Tight junction, Viral myocarditis
• Gene Summary: 'Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development. [provided by RefSeq, Jul 2008]'