GABRR2 (NM_002043) Human Tagged ORF Clone

CAT#: RG218663

GABRR2 (GFP-tagged) - Human gamma-aminobutyric acid (GABA) receptor, rho 2 (GABRR2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GABRR2
• Synonyms: GABA-C receptor, rho-2 subunit; gamma-aminobutyric acid (GABA) receptor, rho 2; OTTHUMP00000040625
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG218663 representing NM_002043
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTTATTTTACAAGACTCATTTTGTTCTTGTTTTGCTTGATGGTTCTCGTGGAGAGCAGAAAACCCA
  AGAGGAAGCGATGGACAGGGCAGGTGGAAATGCCCAAGCCAAGTCACTTATATAAGAAGAACCTTGATGT
  GACCAAGATCCGGAAGGGAAAGCCTCAGCAGCTTCTCAGAGTGGACGAGCACGACTTCAGCATGAGACCC
  GCCTTCGGAGGCCCTGCCATCCCGGTGGGCGTGGACGTACAGGTGGAGAGCCTGGACAGCATCTCCGAGG
  TGGACATGGACTTCACTATGACCCTGTACCTGCGGCATTACTGGAAGGATGAGAGGCTAGCTTTCTCCAG
  CGCCAGCAACAAGAGCATGACCTTCGATGGCCGGCTGGTGAAGAAGATCTGGGTCCCTGATGTCTTCTTT
  GTTCACTCCAAAAGATCGTTCACTCATGACACCACCACTGACAACATCATGCTGAGGGTGTTCCCAGATG
  GACACGTGCTGTACAGCATGAGGATTACGGTCACTGCCATGTGCAACATGGACTTCAGCCACTTTCCCCT
  GGACTCCCAGACCTGTTCTTTGGAGCTGGAGAGCTATGCCTATACAGATGAAGATCTAATGCTGTACTGG
  AAGAATGGGGATGAATCCCTAAAAACAGATGAGAAGATCTCCTTGTCTCAGTTTCTGATTCAGAAATTTC
  ACACAACTTCCAGGCTGGCCTTCTACAGCAGCACTGGCTGGTACAACCGTCTGTACATTAACTTCACGTT
  GCGTCGCCACATCTTCTTCTTCTTGCTCCAAACATATTTCCCTGCCACTCTGATGGTCATGCTGTCCTGG
  GTGTCCTTCTGGATCGACCGCAGAGCTGTGCCTGCCAGAGTTTCACTGGGTATCACGACGGTGCTGACCA
  TGACCACCATCATCACGGGCGTGAATGCCTCCATGCCGCGCGTCTCCTACGTCAAGGCCGTGGACATCTA
  CCTCTGGGTCAGCTTTGTGTTCGTGTTCCTCTCGGTGCTGGAGTATGCGGCTGTCAACTACCTGACCACC
  GTGCAGGAGCGCAAGGAACGGAAGCTGCGGGAGAAGTTCCCGTGCATGTGTGGAATGCTTCATTCAAAAA
  CCATGATGCTGGATGGAAGCTACAGTGAGTCTGAGGCCAACAGCCTGGCTGGGTACCCCAGAAGCCATAT
  CCTGACAGAAGAAGAAAGGCAAGACAAAATAGTGGTCCACCTGGGCCTGAGTGGTGAAGCCAACGCTGCC
  AGAAAGAAGGGGCTTCTGAAGGGCCAGACGGGTTTTCGTATCTTCCAGAATACCCATGCCATTGACAAAT
  ACTCTAGGTTGATATTCCCTGCCTCCTACATATTTTTCAACTTAATTTATTGGTCAGTGTTTTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG218663 representing NM_002043
  Red=Cloning site Green=Tags(s)
  
  MPYFTRLILFLFCLMVLVESRKPKRKRWTGQVEMPKPSHLYKKNLDVTKIRKGKPQQLLRVDEHDFSMRP
  AFGGPAIPVGVDVQVESLDSISEVDMDFTMTLYLRHYWKDERLAFSSASNKSMTFDGRLVKKIWVPDVFF
  VHSKRSFTHDTTTDNIMLRVFPDGHVLYSMRITVTAMCNMDFSHFPLDSQTCSLELESYAYTDEDLMLYW
  KNGDESLKTDEKISLSQFLIQKFHTTSRLAFYSSTGWYNRLYINFTLRRHIFFFLLQTYFPATLMVMLSW
  VSFWIDRRAVPARVSLGITTVLTMTTIITGVNASMPRVSYVKAVDIYLWVSFVFVFLSVLEYAAVNYLTT
  VQERKERKLREKFPCMCGMLHSKTMMLDGSYSESEANSLAGYPRSHILTEEERQDKIVVHLGLSGEANAA
  RKKGLLKGQTGFRIFQNTHAIDKYSRLIFPASYIFFNLIYWSVFS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002043
• ORF Size: 1395 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002043.2, NP_002034.2
• RefSeq Size: 1631 bp
• RefSeq ORF: 1398 bp
• Locus ID: 2570
• Cytogenetics: 6q15
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: 'Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA receptors, which are ligand-gated chloride channels. The protein encoded by this gene is a member of the rho subunit family and is a component of the GABA type A receptor complex. This gene exists on chromosome 6q next to the gene encoding the rho 1 subunit of the GABA type A receptor, in a region thought to be associated with susceptibility for psychiatric disorders and epilepsy. Polymorphisms in this gene may also be associated with alcohol dependence, and general cognitive ability. [provided by RefSeq, Apr 2016]'