ATP dependent metalloprotease YME1L1 (YME1L1) (NM_139312) Human Tagged ORF Clone

CAT#: RG218760

YME1L1 (GFP-tagged) - Human YME1-like 1 (S. cerevisiae) (YME1L1), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: YME1L1
• Synonyms: FTSH; MEG4; OPA11; PAMP; YME1L
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG218760 representing NM_139312
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTTCCTTGTCGAGCACGGTGCAACCCCAGGTTACAGTTCCTCTGAGTCATCTCATCAATGCCTTCC
  ATACACCAAAAAACACTTCTGTTTCTCTCAGTGGAGTGTCAGTTTCTCAAAACCAGCATCGAGATGTAGT
  TCCTGAGCATGAGGCTCCCAGCAGTGAGTGTATGTTCAGTGACTTCCTGACGAAGCTTAACATTGTTTCA
  ATTGGCAAAGGAAAAATATTCGAAGGGTACAGATCCATGTTCATGGAGCCAGCAAAAAGGATGAAGAAGA
  GCTTGGACACAACCGATAACTGGCACATCCGTCCAGAACCCTTCTCCCTCTCAATCCCTCCTTCACTTAA
  CTTAAGGGACCTTGGATTATCTGAACTAAAAATTGGACAGATTGATCAGCTGGTAGAAAATCTACTTCCT
  GGATTTTGTAAAGGCAAAAACATTTCTTCCCATTGGCATACATCCCATGTCTCTGCACAATCCTTCTTTG
  AAAATAAATATGGTAACTTAGATATATTTAGTACATTACGTTCCTCTTGCTTGTATCGACATCATTCAAG
  AGCTCTTCAAAGCATTTGTTCAGATCTTCAGTACTGGCCAGTTTTCATACAGTCTCGGGGTTTTAAAACT
  TTGAAATCAAGGACACGACGTCTCCAGTCTACCTCCGAGAGATTAGCTGAAACACAGAATATAGCGCCAT
  CATTCGTGAAGGGGTTTCTTTTGCGGGACAGAGGATCAGATGTTGAGAGTTTGGACAAACTCATGAAAAC
  CAAAAATATACCTGAAGCTCACCAAGATGCATTTAAAACTGGTTTTGCGGAAGGTTTTCTGAAAGCTCAA
  GCACTCACACAAAAAACCAATGATTCCCTAAGGCGAACCCGTCTGATTCTCTTCGTTCTGCTGCTATTCG
  GCATTTATGGACTTCTAAAAAACCCATTTTTATCTGTCCGCTTCCGGACAACAACAGGGCTTGATTCTGC
  AGTAGATCCTGTCCAGATGAAAAATGTCACCTTTGAACATGTTAAAGGGGTGGAGGAAGCTAAACAAGAA
  TTACAGGAAGTTGTTGAATTCTTGAAAAATCCACAAAAATTTACTATTCTTGGAGGTAAACTTCCAAAAG
  GAATTCTTTTAGTTGGACCCCCAGGGACTGGAAAGACACTTCTTGCCCGAGCTGTGGCGGGAGAAGCTGA
  TGTTCCTTTTTATTATGCTTCTGGATCCGAATTTGATGAGATGTTTGTGGGTGTGGGAGCCAGCCGTATC
  AGAAATCTTTTTAGGGAAGCAAAGGCGAATGCTCCTTGTGTTATATTTATTGATGAATTAGATTCTGTTG
  GTGGGAAGAGAATTGAATCTCCAATGCATCCATATTCAAGGCAGACCATAAATCAACTTCTTGCTGAAAT
  GGATGGTTTTAAACCCAATGAAGGAGTTATCATAATAGGAGCCACAAACTTCCCAGAGGCATTAGATAAT
  GCCTTAATACGTCCTGGTCGTTTTGACATGCAAGTTACAGTTCCAAGGCCAGATGTAAAAGGTCGAACAG
  AAATTTTGAAATGGTATCTCAATAAAATAAAGTTTGATCAATCCGTTGATCCAGAAATTATAGCTCGAGG
  TACTGTTGGCTTTTCCGGAGCAGAGTTGGAGAATCTTGTGAACCAGGCTGCATTAAAAGCAGCTGTTGAT
  GGAAAAGAAATGGTTACCATGAAGGAGCTGGAGTTTTCCAAAGACAAAATTCTAATGGGGCCTGAAAGAA
  GAAGTGTGGAAATTGATAACAAAAACAAAACCATCACAGCATATCATGAATCTGGTCATGCCATTATTGC
  ATATTACACAAAAGATGCAATGCCTATCAACAAAGCTACAATCATGCCACGGGGGCCAACACTTGGACAT
  GTGTCCCTGTTACCTGAGAATGACAGATGGAATGAAACTAGAGCCCAGCTGCTTGCACAAATGGATGTTA
  GTATGGGAGGAAGAGTGGCAGAGGAGCTTATATTTGGAACCGACCATATTACAACAGGTGCTTCCAGTGA
  TTTTGATAATGCCACTAAAATAGCAAAGCGGATGGTTACCAAATTTGGAATGAGTGAAAAGCTTGGAGTT
  ATGACCTACAGTGATACAGGGAAACTAAGTCCAGAAACCCAATCTGCCATCGAACAAGAAATAAGAATCC
  TTCTAAGGGACTCATATGAACGAGCAAAACATATCTTGAAAACTCATGCAAAGGAGCATAAGAATCTCGC
  AGAAGCTTTATTGACCTATGAGACTTTGGATGCCAAAGAGATTCAAATTGTTCTTGAGGGGAAAAAGTTG
  GAAGTGAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG218760 representing NM_139312
  Red=Cloning site Green=Tags(s)
  
  MFSLSSTVQPQVTVPLSHLINAFHTPKNTSVSLSGVSVSQNQHRDVVPEHEAPSSECMFSDFLTKLNIVS
  IGKGKIFEGYRSMFMEPAKRMKKSLDTTDNWHIRPEPFSLSIPPSLNLRDLGLSELKIGQIDQLVENLLP
  GFCKGKNISSHWHTSHVSAQSFFENKYGNLDIFSTLRSSCLYRHHSRALQSICSDLQYWPVFIQSRGFKT
  LKSRTRRLQSTSERLAETQNIAPSFVKGFLLRDRGSDVESLDKLMKTKNIPEAHQDAFKTGFAEGFLKAQ
  ALTQKTNDSLRRTRLILFVLLLFGIYGLLKNPFLSVRFRTTTGLDSAVDPVQMKNVTFEHVKGVEEAKQE
  LQEVVEFLKNPQKFTILGGKLPKGILLVGPPGTGKTLLARAVAGEADVPFYYASGSEFDEMFVGVGASRI
  RNLFREAKANAPCVIFIDELDSVGGKRIESPMHPYSRQTINQLLAEMDGFKPNEGVIIIGATNFPEALDN
  ALIRPGRFDMQVTVPRPDVKGRTEILKWYLNKIKFDQSVDPEIIARGTVGFSGAELENLVNQAALKAAVD
  GKEMVTMKELEFSKDKILMGPERRSVEIDNKNKTITAYHESGHAIIAYYTKDAMPINKATIMPRGPTLGH
  VSLLPENDRWNETRAQLLAQMDVSMGGRVAEELIFGTDHITTGASSDFDNATKIAKRMVTKFGMSEKLGV
  MTYSDTGKLSPETQSAIEQEIRILLRDSYERAKHILKTHAKEHKNLAEALLTYETLDAKEIQIVLEGKKL
  EVR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139312
• ORF Size: 2319 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_139312.1, NP_647473.1
• RefSeq Size: 4036
• RefSeq ORF: 2322
• Locus ID: 10730
• Domains: Peptidase_M41, AAA
• Protein Families: Druggable Genome, Protease
• Gene Summary: The protein encoded by this gene is the human ortholog of yeast mitochondrial AAA metalloprotease, Yme1p. It is localized in the mitochondria and can functionally complement a yme1 disruptant yeast strain. It is proposed that this gene plays a role in mitochondrial protein metabolism and could be involved in mitochondrial pathologies. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]