LRRK1 (NM_024652) Human Tagged ORF Clone

CAT#: RG218814

LRRK1 (GFP-tagged) - Human leucine-rich repeat kinase 1 (LRRK1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LRRK1
• Synonyms: RIPK6; Roco1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG218814 representing NM_024652
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGCATGTCGCAAAGACCCCCCAGCATGTACTGGTGTGTGGGGCCGGAGGAGTCAGCTGTGTGTC
  CAGAACGTGCCATGGAGACGCTTAACGGTGCCGGGGACACGGGCGGCAAGCCGTCCACGCGGGGCGGTGA
  CCCTGCAGCGCGGTCCCGCAGGACGGAAGGCATCCGCGCCGCGTACAGGCGGGGAGACCGCGGCGGCGCC
  CGGGACCTGCTGGAGGAGGCCTGCGACCAGTGCGCGTCCCAGCTGGAAAAGGGCCAGCTTCTGAGCATCC
  CGGCAGCCTATGGGGATCTGGAGATGGTCCGCTACCTACTCAGCAAGAGACTGGTGGAGCTGCCCACCGA
  GCCCACGGATGACAACCCAGCCGTGGTGGCAGCGTATTTTGGACACACGGCAGTTGTGCAGGAATTGCTT
  GAGTCCTTACCAGGTCCCTGCAGTCCCCAGCGGCTTCTGAACTGGATGCTGGCCTTGGCTTGCCAGCGAG
  GGCACCTGGGGGTTGTGAAGCTCCTGGTCCTGACGCACGGGGCTGACCCGGAGAGCTACGCTGTCAGGAA
  GAATGAGTTCCCTGTCATCGTGCGCTTGCCCCTGTATGCGGCCATCAAGTCAGGGAATGAAGACATTGCA
  ATATTCCTGCTTCGGCATGGGGCCTATTTCTGTTCCTACATCTTGCTGGATAGTCCTGACCCCAGCAAAC
  ATCTGCTGAGAAAGTACTTCATTGAAGCCAGTCCCTTGCCCAGCAGTTATCCGGGAAAAACAGCTCTCCG
  TGTGAAATGGTCCCATCTCAGACTGCCCTGGGTAGACCTAGACTGGCTCATAGACATCTCCTGCCAGATC
  ACGGAGCTCGACCTTTCTGCCAACTGCCTGGCGACCCTCCCCTCGGTTATCCCCTGGGGCCTCATCAATC
  TCCGGAAGCTGAACCTCTCCGACAACCACCTGGGGGAGCTGCCTGGCGTGCAGTCATCGGACGAAATCAT
  CTGTTCCAGGCTACTTGAAATTGACATTTCCAGCAACAAGTTGTCCCACCTCCCTCCTGGATTCTTGCAC
  CTCTCAAAACTTCAAAAACTGACAGCTTCAAAAAATTGTTTAGAAAAATTGTTCGAAGAAGAAAATGCCA
  CTAACTGGATAGGTTTACGGAAGCTACAGGAACTTGATATATCTGACAATAAATTGACAGAACTCCCTGC
  CCTGTTCCTTCACTCTTTCAAGTCCCTCAATTCTCTGAATGTCTCCAGAAACAACCTGAAGGTGTTTCCA
  GATCCCTGGGCCTGCCCTTTGAAATGTTGTAAAGCTTCCAGAAATGCCCTGGAATGTCTGCCAGACAAAA
  TGGCTGTCTTTTGGAAAAATCACCTGAAGGATGTGGATTTCTCAGAAAACGCACTCAAAGAAGTTCCCCT
  GGGACTTTTCCAGCTTGATGCCCTCATGTTCTTGAGGTTACAGGGGAACCAGCTGGCGGCACTTCCACCT
  CAAGAGAAGTGGACCTGCAGGCAGCTCAAAACCCTGGATCTCTCCAGAAACCAACTTGGCAAAAATGAAG
  ATGGACTGAAAACGAAGCGTATTGCCTTTTTCACCACCAGAGGTCGCCAGCGCTCCGGGACTGAGGCAGC
  AAGTGTGCTGGAATTTCCGGCCTTCCTAAGTGAGTCTTTGGAAGTCCTTTGCCTGAACGACAACCACCTC
  GACACAGTCCCTCCCTCGGTTTGCCTACTGAAGAGCTTATCAGAGCTCTACTTGGGAAACAACCCTGGCC
  TCCGGGAGCTCCCTCCTGAGCTGGGGCAGCTGGGCAACCTCTGGCAGCTGGACACTGAAGACCTGACCAT
  CAGCAATGTGCCTGCAGAAATCCAAAAAGAAGGCCCCAAAGCAATGCTGTCTTACCTGCGTGCTCAGCTG
  CGGAAAGCGGAAAAGTGCAAGCTGATGAAGATGATCATCGTGGGTCCCCCGCGCCAGGGCAAGTCCACCC
  TCCTGGAGATCTTACAGACGGGGAGGGCCCCCCAGGTGGTGCATGGAGAGGCCACCATCAGGACCACCAA
  GTGGGAGCTCCAGAGGCCGGCTGGCTCAAGAGCCAAGGTTGAGTCCGTGGAGTTCAACGTCTGGGACATC
  GGGGGACCGGCCAGCATGGCCACTGTCAACCAGTGCTTCTTCACGGACAAGGCCCTGTACGTGGTGGTCT
  GGAACCTGGCGCTGGGGGAGGAGGCCGTGGCCAACCTCCAGTTCTGGCTGCTCAACATCGAGGCCAAGGC
  CCCAAACGCCGTGGTGCTGGTGGTCGGGACGCACCTGGATTTAATTGAAGCCAAGTTCCGTGTGGAAAGG
  ATTGCAACGCTGCGTGCCTATGTGCTGGCACTCTGCCGCTCCCCCTCCGGCTCCAGGGCCACAGGCTTCC
  CAGACATCACCTTCAAACACTTACATGAGATTTCCTGCAAGAGCCTGGAAGGTCAGGAAGGGCTGCGACA
  GCTGATTTTCCACGTCACGTGCAGCATGAAGGACGTGGGCAGCACCATCGGCTGCCAGCGACTGGCAGGG
  CGGCTGATCCCCAGGAGCTACCTGAGCCTGCAGGAGGCCGTGCTGGCAGAGCAGCAGCGCCGCAGCCGGG
  ACGACGACGTGCAGTACCTGACGGACAGGCAGCTGGAGCAGCTGGTGGAGCAGACGCCCGACAACGACAT
  CAAGGACTACGAGGACCTGCAGTCAGCCATCAGCTTCCTCATAGAAACCGGCACCCTGCTCCATTTCCCG
  GACACCAGCCACGGCCTGAGGAACCTCTACTTCCTCGACCCTATTTGGCTCTCCGAATGTCTGCAGAGGA
  TCTTTAATATTAAGGGCTCTCGGTCAGTGGCCAAGAATGGGGTGATCAGAGCAGAAGACCTCAGGATGCT
  GCTGGTGGGGACTGGCTTCACGCAGCAGACGGAAGAGCAGTACTTCCAGTTCCTGGCCAAGTTTGAGATC
  GCCCTGCCCGTCGCCAATGACAGCTACCTCCTGCCCCATCTCCTTCCATCTAAACCTGGCCTGGACACCC
  ACGGTATGCGGCACCCCACAGCCAACACCATTCAGAGGGTATTTAAGATGAGCTTCGTTCCCGTTGGCTT
  CTGGCAAAGGTTTATAGCACGGATGCTGATCAGCCTGGCGGAGATGGACCTGCAGCTTTTTGAAAACAAG
  AAGAATACTAAAAGCAGGAACAGGAAAGTCACCATTTACAGTTTTACAGGAAACCAGAGAAATCGCTGTA
  GCACATTCAGAGTGAAAAGAAATCAGACCATCTATTGGCAGGAAGGGCTCCTGGTCACTTTTGATGGGGG
  CTACCTCAGTGTGGAATCTTCCGACGTGAACTGGAAAAAGAAGAAAAGCGGAGGAATGAAAATTGTTTGC
  CAATCAGAAGTGAGGGACTTCTCAGCCATGGCTTTCATCACGGACCACGTCAATTCCTTGATTGATCAGT
  GGTTTCCCGCCCTGACAGCCACAGAGAGCGACGGGACGCCACTCATGGAGCAGTACGTGCCCTGCCCGGT
  CTGCGAGACAGCCTGGGCCCAGCACACGGACCCCAGTGAGAAATCAGAGGATGTGCAGTACTTCGACATG
  GAAGACTGTGTCCTGACGGCCATCGAGCGGGACTTCATCTCCTGCCCCAGACACCCGGACCTCCCCGTGC
  CGCTGCAGGAGCTGGTCCCTGAACTGTTCATGACCGACTTCCCGGCCAGGCTCTTCCTGGAGAACAGCAA
  GCTGGAGCACAGCGAGGACGAGGGCAGCGTCCTGGGCCAGGGCGGCAGTGGCACCGTCATCTACCGGGCC
  CGGTACCAGGGCCAGCCTGTGGCCGTCAAGCGCTTCCACATCAAAAAATTCAAGAACTTTGCTAACGTAC
  CGGCAGACACCATGCTGAGGCACCTGCGGGCCACCGATGCCATGAAGAACTTCTCCGAGTTCCGGCAGGA
  GGCCAGCATGCTGCACGCGCTGCAGCACCCCTGCATCGTGGCGCTCATCGGCATCAGCATCCACCCGCTC
  TGCTTCGCCCTGGAGCTCGCGCCGCTCAGCAGCCTCAACACCGTGCTGTCCGAGAACGCCAGAGATTCTT
  CCTTTATACCCCTGGGACACATGCTCACCCAAAAAATAGCCTACCAGATCGCCTCGGGCCTGGCCTACCT
  GCACAAGAAAAACATCATCTTCTGTGACCTGAAGTCGGACAACATTCTGGTGTGGTCCCTTGACGTCAAG
  GAGCACATCAACATCAAGCTATCTGACTACGGGATTTCGAGGCAGTCATTCCATGAGGGCGCCCTAGGCG
  TGGAGGGCACTCCTGGCTACCAGGCCCCAGAGATCAGGCCTCGCATTGTATATGATGAGAAGGTAGATAT
  GTTCTCCTATGGAATGGTGCTCTACGAGTTGCTGTCAGGACAGCGCCCTGCACTGGGCCACCACCAGCTC
  CAGATTGCCAAGAAGCTGTCCAAGGGCATCCGCCCGGTTCTGGGGCAGCCGGAGGAAGTGCAGTTCCGGC
  GACTGCAGGCGCTCATGATGGAGTGCTGGGACACTAAGCCAGAGAAGCGACCGCTGGCCCTGTCGGTGGT
  GAGCCAGATGAAGGACCCGACTTTTGCCACCTTCATGTATGAACTGTGCTGTGGGAAGCAGACAGCCTTC
  TTCTCATCCCAGGGCCAGGAGTACACCGTGGTGTTTTGGGATGGAAAAGAGGAGTCCAGGAACTACACGG
  TGGTGAACACAGAGAAGGGCCTCATGGAGGTGCAGAGGATGTGCTGCCCTGGGATGAAGGTGAGCTGCCA
  GCTCCAGGTCCAGAGATCCCTGTGGACAGCCACCGAGGACCAGAAAATCTACATCTACACCCTCAAGGGC
  ATGTGCCCCTTAAACACACCCCAACAGGCCTTGGATACTCCAGCTGTCGTCACCTGCTTCTTGGCCGTGC
  CTGTTATTAAAAAGAATTCCTACCTGGTCTTAGCGGGCCTCGCCGATGGGCTTGTGGCTGTGTTTCCCGT
  GGTGCGGGGCACCCCAAAGGACAGCTGCTCCTACCTGTGCTCACACACAGCCAACAGGTCCAAGTTCAGC
  ATCGCGGATGAAGACGCACGGCAGAACCCCTACCCAGTGAAGGCCATGGAGGTGGTCAACAGCGGCTCTG
  AGGTCTGGTACAGCAATGGGCCGGGCCTCCTTGTCATCGACTGTGCCTCCCTGGAGATCTGCAGGCGGCT
  GGAGCCCTACATGGCCCCCTCCATGGTTACGTCAGTCGTGTGCAGCTCTGAGGGCAGAGGGGAGGAGGTC
  GTCTGGTGCCTGGATGACAAGGCCAACTCCTTGGTGATGTACCACTCCACCACCTACCAGCTGTGTGCCC
  GGTACTTCTGCGGGGTCCCCAGCCCCCTCAGGGACATGTTTCCCGTGCGGCCCTTGGACACGGAACCCCC
  GGCAGCCAGCCACACGGCCAACCCAAAGGTGCCTGAGGGGGACTCCATCGCGGACGTGAGCATCATGTAC
  AGTGAGGAGCTGGGCACGCAGATCCTGATCCACCAGGAATCACTCACTGACTACTGCTCCATGTCCTCCT
  ACTCCTCATCCCCACCCCGCCAGGCTGCCAGGTCCCCCTCAAGCCTCCCCAGCTCCCCAGCAAGTTCTTC
  CAGTGTGCCTTTCTCCACCGACTGCGAGGACTCAGACATGCTACATACGCCCGGTGCTGCCTCCGACAGG
  TCTGAGCATGACCTGACCCCCATGGACGGGGAGACCTTCAGCCAGCACCTGCAGGCCGTGAAGATCCTCG
  CCGTCAGAGACCTCATTTGGGTCCCCAGGCGCGGTGGAGATGTTATCGTCATTGGCCTGGAGAAGGATTC
  TGGCGCCCAGCGGGGCCGAGTCATTGCCGTCTTAAAAGCCCGAGAGCTGACTCCGCATGGGGTGCTGGTG
  GATGCTGCCGTGGTGGCAAAGGACACTGTTGTGTGCACCTTTGAAAATGAAAACACAGAGTGGTGCCTGG
  CCGTCTGGAGGGGCTGGGGCGCCAGGGAGTTCGACATTTTCTACCAGTCCTACGAGGAGCTGGGCCGGCT
  GGAGGCTTGCACTCGCAAGAGAAGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG218814 representing NM_024652
  Red=Cloning site Green=Tags(s)
  
  MAGMSQRPPSMYWCVGPEESAVCPERAMETLNGAGDTGGKPSTRGGDPAARSRRTEGIRAAYRRGDRGGA
  RDLLEEACDQCASQLEKGQLLSIPAAYGDLEMVRYLLSKRLVELPTEPTDDNPAVVAAYFGHTAVVQELL
  ESLPGPCSPQRLLNWMLALACQRGHLGVVKLLVLTHGADPESYAVRKNEFPVIVRLPLYAAIKSGNEDIA
  IFLLRHGAYFCSYILLDSPDPSKHLLRKYFIEASPLPSSYPGKTALRVKWSHLRLPWVDLDWLIDISCQI
  TELDLSANCLATLPSVIPWGLINLRKLNLSDNHLGELPGVQSSDEIICSRLLEIDISSNKLSHLPPGFLH
  LSKLQKLTASKNCLEKLFEEENATNWIGLRKLQELDISDNKLTELPALFLHSFKSLNSLNVSRNNLKVFP
  DPWACPLKCCKASRNALECLPDKMAVFWKNHLKDVDFSENALKEVPLGLFQLDALMFLRLQGNQLAALPP
  QEKWTCRQLKTLDLSRNQLGKNEDGLKTKRIAFFTTRGRQRSGTEAASVLEFPAFLSESLEVLCLNDNHL
  DTVPPSVCLLKSLSELYLGNNPGLRELPPELGQLGNLWQLDTEDLTISNVPAEIQKEGPKAMLSYLRAQL
  RKAEKCKLMKMIIVGPPRQGKSTLLEILQTGRAPQVVHGEATIRTTKWELQRPAGSRAKVESVEFNVWDI
  GGPASMATVNQCFFTDKALYVVVWNLALGEEAVANLQFWLLNIEAKAPNAVVLVVGTHLDLIEAKFRVER
  IATLRAYVLALCRSPSGSRATGFPDITFKHLHEISCKSLEGQEGLRQLIFHVTCSMKDVGSTIGCQRLAG
  RLIPRSYLSLQEAVLAEQQRRSRDDDVQYLTDRQLEQLVEQTPDNDIKDYEDLQSAISFLIETGTLLHFP
  DTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIRAEDLRMLLVGTGFTQQTEEQYFQFLAKFEI
  ALPVANDSYLLPHLLPSKPGLDTHGMRHPTANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENK
  KNTKSRNRKVTIYSFTGNQRNRCSTFRVKRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGMKIVC
  QSEVRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCETAWAQHTDPSEKSEDVQYFDM
  EDCVLTAIERDFISCPRHPDLPVPLQELVPELFMTDFPARLFLENSKLEHSEDEGSVLGQGGSGTVIYRA
  RYQGQPVAVKRFHIKKFKNFANVPADTMLRHLRATDAMKNFSEFRQEASMLHALQHPCIVALIGISIHPL
  CFALELAPLSSLNTVLSENARDSSFIPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLDVK
  EHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGHHQL
  QIAKKLSKGIRPVLGQPEEVQFRRLQALMMECWDTKPEKRPLALSVVSQMKDPTFATFMYELCCGKQTAF
  FSSQGQEYTVVFWDGKEESRNYTVVNTEKGLMEVQRMCCPGMKVSCQLQVQRSLWTATEDQKIYIYTLKG
  MCPLNTPQQALDTPAVVTCFLAVPVIKKNSYLVLAGLADGLVAVFPVVRGTPKDSCSYLCSHTANRSKFS
  IADEDARQNPYPVKAMEVVNSGSEVWYSNGPGLLVIDCASLEICRRLEPYMAPSMVTSVVCSSEGRGEEV
  VWCLDDKANSLVMYHSTTYQLCARYFCGVPSPLRDMFPVRPLDTEPPAASHTANPKVPEGDSIADVSIMY
  SEELGTQILIHQESLTDYCSMSSYSSSPPRQAARSPSSLPSSPASSSSVPFSTDCEDSDMLHTPGAASDR
  SEHDLTPMDGETFSQHLQAVKILAVRDLIWVPRRGGDVIVIGLEKDSGAQRGRVIAVLKARELTPHGVLV
  DAAVVAKDTVVCTFENENTEWCLAVWRGWGAREFDIFYQSYEELGRLEACTRKRR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024652
• ORF Size: 6045 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_024652.3, NP_078928.3
• RefSeq Size: 7647
• RefSeq ORF: 6048
• Locus ID: 79705
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a multi-domain protein that is a leucine-rich repeat kinase and a GDP/GTP binding protein. The encoded protein is thought to play a role in the regulation of bone mass. Mice lacking a similar gene showed severe osteopetrosis, increased bone mineralization and decreased bone resorption. [provided by RefSeq, Jan 2017]