MTGR1 (CBFA2T2) (NM_001032999) Human Tagged ORF Clone

CAT#: RG218940

CBFA2T2 (GFP-tagged) - Human core-binding factor, runt domain, alpha subunit 2; translocated to, 2 (CBFA2T2), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CBFA2T2
• Synonyms: EHT; MTGR1; p85; ZMYND3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG218940 representing NM_001032999
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTAGGCGTCCCTGGAGCGGCCGCCTTCCAGCTTGGTCCTGAGAAAAGGGTGCCAGCGATGCCTGGAT
  CGCCTGTGGAAGTGAAGATACAGTCCAGATCCTCACCTCCCACCATGCCACCCCTCCCACCAATAAATCC
  TGGAGGACCGAGGCCAGTGTCCTTCACTCCTACTGCATTAAGCAATGGCATCAACCATTCTCCTCCTACC
  CTGAATGGTGCCCCATCACCGCCACAGAGATTCAGCAATGGTCCTGCCTCCTCCACATCATCTGCACTCA
  CAAATCAGCAATTGCCAGCCACTTGTGGTGCTCGACAACTCAGCAAGTTGAAACGCTTTCTTACCACTCT
  GCAACAGTTTGGCAATGACATCTCCCCTGAGATTGGGGAGAAGGTGCGGACTCTTGTTCTTGCACTGGTG
  AACTCAACAGTGACAATTGAGGAATTCCACTGTAAGCTCCAAGAAGCCACAAACTTTCCCCTTCGTCCTT
  TTGTGATTCCATTTCTCAAGGCCAACCTGCCCCTGCTGCAGCGGGAACTGCTGCACTGCGCTCGGGCGGC
  CAAGCAGACCCCATCCCAGTACCTGGCTCAGCACGAACACCTTCTGCTCAACACAAGCATTGCATCGCCT
  GCTGACTCGTCAGAGTTGCTCATGGAGGTGCACGGAAATGGGAAGAGGCCCAGTCCAGAGAGGAGAGAAG
  AGAATAGTTTTGATAGAGACACAATTGCTCCTGAGCCTCCTGCCAAGAGAGTATGTACCATCAGCCCTGC
  TCCTCGGCACAGTCCTGCTCTCACTGTGCCCCTCATGAATCCCGGGGGCCAATTCCATCCTACCCCTCCA
  CCTCTTCAGCATTACACCTTAGAGGATATTGCAACTTCTCACCTGTATCGGGAACCCAACAAGATGCTAG
  AGCATCGAGAAGTTCGTGATAGACACCACAGTCTTGGTCTAAATGGAGGCTATCAAGATGAGTTGGTAGA
  TCATCGTTTGACAGAAAGGGAATGGGCTGATGAATGGAAACATCTTGACCATGCGCTGAATTGCATTATG
  GAAATGGTAGAGAAAACAAGGCGCTCTATGGCAGTTCTGCGGCGCTGTCAGGAATCAGATCGTGAAGAAC
  TCAACTACTGGAAAAGACGGTACAATGAAAACACAGAGCTGAGGAAAACGGGGACCGAGTTGGTCTCCAG
  GCAGCACAGCCCTGGGAGTGCAGATTCTCTCAGCAATGATTCTCAGAGAGAGTTCAACAGCAGGCCAGGT
  ACAGGATACGTACCTGTGGAGTTTTGGAAAAAAACAGAAGAAGCTGTGAATAAGGTGAAAATTCAGGCCA
  TGTCAGAAGTACAGAAGGCCGTCGCTGAGGCAGAGCAGAAAGCCTTTGAAGTGATTGCAACAGAGAGAGC
  ACGAATGGAGCAAACCATAGCGGATGTCAAGCGGCAGGCCGCAGAGGATGCTTTCCTCGTCATCAATGAG
  CAAGAGGAGTCCACGGAGAACTGCTGGAACTGTGGCCGCAAAGCCAGCGAGACATGCAGTGGCTGCAATA
  TCGCGCGATACTGTGGCTCTTTCTGCCAGCACAAGGACTGGGAGCGGCACCACCGCCTCTGTGGTCAGAA
  CCTGCATGGCCAGAGCCCCCACGGCCAGGGCCGGCCGCTGCTTCCTGTAGGCAGGGGCTCCTCTGCCAGG
  TCCGCCGACTGCAGCGTGCCCAGCCCAGCCCTCGACAAGACCTCGGCAACCACATCGCGTTCCTCAACAC
  CTGCTTCTGTGACAGCTATCGACACCAACGGACTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG218940 representing NM_001032999
  Red=Cloning site Green=Tags(s)
  
  MVGVPGAAAFQLGPEKRVPAMPGSPVEVKIQSRSSPPTMPPLPPINPGGPRPVSFTPTALSNGINHSPPT
  LNGAPSPPQRFSNGPASSTSSALTNQQLPATCGARQLSKLKRFLTTLQQFGNDISPEIGEKVRTLVLALV
  NSTVTIEEFHCKLQEATNFPLRPFVIPFLKANLPLLQRELLHCARAAKQTPSQYLAQHEHLLLNTSIASP
  ADSSELLMEVHGNGKRPSPERREENSFDRDTIAPEPPAKRVCTISPAPRHSPALTVPLMNPGGQFHPTPP
  PLQHYTLEDIATSHLYREPNKMLEHREVRDRHHSLGLNGGYQDELVDHRLTEREWADEWKHLDHALNCIM
  EMVEKTRRSMAVLRRCQESDREELNYWKRRYNENTELRKTGTELVSRQHSPGSADSLSNDSQREFNSRPG
  TGYVPVEFWKKTEEAVNKVKIQAMSEVQKAVAEAEQKAFEVIATERARMEQTIADVKRQAAEDAFLVINE
  QEESTENCWNCGRKASETCSGCNIARYCGSFCQHKDWERHHRLCGQNLHGQSPHGQGRPLLPVGRGSSAR
  SADCSVPSPALDKTSATTSRSSTPASVTAIDTNGL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001032999
• ORF Size: 1785 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001032999.1, NP_001028171.1
• RefSeq Size: 6517
• RefSeq ORF: 1788
• Locus ID: 9139
• Protein Families: Transcription Factors
• Gene Summary: In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described. [provided by RefSeq, Jul 2008]