DOC2B (NM_003585) Human Tagged ORF Clone

CAT#: RG218949

DOC2B (GFP-tagged) - Human double C2-like domains, beta (DOC2B)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DOC2B
• Synonyms: DOC2BL
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG218949 representing NM_003585
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCTCCGGCGGCGCGGGGAGAAGGCGACCATCAGCATCCAGGAGCATATGGCCATCGACGTGTGCC
  CCGGCCCCATCCGTCCCATCAAGCAGATCTCCGACTACTTCCCCCGCTTCCCGCGGGGCCTGCCCCCGGA
  CGCCGGGCCCCGAGCCGCTGCACCCCCGGACGCCCCCGCGCGCCCGGCTGTGGCCGGTGCCGGCCGCCGC
  AGCCCCTCCGACGGCGCCCGCGAGGACGACGAGGATGTGGACCAGCTCTTCGGAGCCTACGGCTCCAGCC
  CGGGCCCCAGCCCGGGTCCCAGCCCCGCGCGGCCGCCAGCCAAGCCGCCGGAGGACGAGCCGGACGCCGA
  CGGCTACGAGTCGGACGACTGCACTGCCCTGGGCACGCTGGACTTCAGCCTGCTGTATGACCAGGAGAAC
  AACGCCCTCCACTGCACCATCACCAAGGCCAAGGGCCTGAAGCCAATGGACCACAATGGGCTGGCAGACC
  CCTACGTCAAGCTGCACCTGCTGCCAGGAGCCAGTAAGGCAAATAAGCTCAGAACAAAAACTCTCCGTAA
  CACTCTGAACCCCACATGGAACGAGACCCTCACTTACTACGGGATCACAGATGAAGACATGATCCGCAAG
  ACCCTGCGGATCTCTGTGTGTGACGAGGACAAATTCCGGCACAATGAGTTCATCGGGGAGACACGTGTGC
  CCCTGAAGAAGCTGAAACCCAACCACACCAAGACCTTCAACATCTGCCTGGAGAAGCAGCTGCCGGTGGA
  CAAGACTGAAGACAAGTCCCTGGAGGAGCGGGGCCGCATCCTCATCTCCCTCAAGTACAGCTCACAGAAG
  CAAGGCCTGCTGGTAGGCATCGTGCGGTGCGCCCACCTGGCCGCCATGGACGCCAACGGCTACTCGGACC
  CCTACGTGAAAACATACCTGAGGCCAGATGTGGACAAGAAATCCAAACATAAGACAGCGGTGAAGAAAAA
  AACCCTGAACCCGGAGTTTAATGAGGAGTTCTGTTACGAGATCAAGCATGGGGACCTGGCCAAGAAGTCC
  CTGGAGGTCACCGTTTGGGATTACGACATTGGAAAATCCAACGATTTCATTGGTGGTGTGGTTCTGGGCA
  TCCACGCCAAGGGGGAGCGCCTGAAGCACTGGTTTGACTGCCTGAAGAACAAGGACAAGCGCATCGAGCG
  CTGGCACACGCTCACCAGCGAGCTCCCAGGGGCTGTGCTCAGCGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG218949 representing NM_003585
  Red=Cloning site Green=Tags(s)
  
  MTLRRRGEKATISIQEHMAIDVCPGPIRPIKQISDYFPRFPRGLPPDAGPRAAAPPDAPARPAVAGAGRR
  SPSDGAREDDEDVDQLFGAYGSSPGPSPGPSPARPPAKPPEDEPDADGYESDDCTALGTLDFSLLYDQEN
  NALHCTITKAKGLKPMDHNGLADPYVKLHLLPGASKANKLRTKTLRNTLNPTWNETLTYYGITDEDMIRK
  TLRISVCDEDKFRHNEFIGETRVPLKKLKPNHTKTFNICLEKQLPVDKTEDKSLEERGRILISLKYSSQK
  QGLLVGIVRCAHLAAMDANGYSDPYVKTYLRPDVDKKSKHKTAVKKKTLNPEFNEEFCYEIKHGDLAKKS
  LEVTVWDYDIGKSNDFIGGVVLGIHAKGERLKHWFDCLKNKDKRIERWHTLTSELPGAVLSD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003585
• ORF Size: 1236 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_003585.1, NP_003576.1
• RefSeq Size: 2030
• RefSeq ORF: 1239
• Locus ID: 8447
• Gene Summary: There are at least two protein isoforms of the Double C2 protein, namely alpha (DOC2A) and beta (DOC2B), which contain two C2-like domains. DOC2A and DOC2B are encoded by different genes; these genes are at times confused with the unrelated DAB2 gene which was initially named DOC-2. DOC2B is expressed ubiquitously and is suggested to be involved in Ca(2+)-dependent intracellular vesicle trafficking in various types of cells. [provided by RefSeq, Jul 2008]