DOC2B (NM_003585) Human Tagged ORF Clone

CAT#: RC218949

DOC2B (Myc-DDK-tagged)-Human double C2-like domains, beta (DOC2B)

Product Images

Western blot validation of overexpression lysate (Cat# LY418555) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218949 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified DOC2B protein (Cat# TP318949). The protein was produced from HEK293T cells transfected with DOC2B cDNA clone (Cat# RC218949) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DOC2B
• Synonyms: DOC2BL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218949 representing NM_003585
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCTCCGGCGGCGCGGGGAGAAGGCGACCATCAGCATCCAGGAGCATATGGCCATCGACGTGTGCC
  CCGGCCCCATCCGTCCCATCAAGCAGATCTCCGACTACTTCCCCCGCTTCCCGCGGGGCCTGCCCCCGGA
  CGCCGGGCCCCGAGCCGCTGCACCCCCGGACGCCCCCGCGCGCCCGGCTGTGGCCGGTGCCGGCCGCCGC
  AGCCCCTCCGACGGCGCCCGCGAGGACGACGAGGATGTGGACCAGCTCTTCGGAGCCTACGGCTCCAGCC
  CGGGCCCCAGCCCGGGTCCCAGCCCCGCGCGGCCGCCAGCCAAGCCGCCGGAGGACGAGCCGGACGCCGA
  CGGCTACGAGTCGGACGACTGCACTGCCCTGGGCACGCTGGACTTCAGCCTGCTGTATGACCAGGAGAAC
  AACGCCCTCCACTGCACCATCACCAAGGCCAAGGGCCTGAAGCCAATGGACCACAATGGGCTGGCAGACC
  CCTACGTCAAGCTGCACCTGCTGCCAGGAGCCAGTAAGGCAAATAAGCTCAGAACAAAAACTCTCCGTAA
  CACTCTGAACCCCACATGGAACGAGACCCTCACTTACTACGGGATCACAGATGAAGACATGATCCGCAAG
  ACCCTGCGGATCTCTGTGTGTGACGAGGACAAATTCCGGCACAATGAGTTCATCGGGGAGACACGTGTGC
  CCCTGAAGAAGCTGAAACCCAACCACACCAAGACCTTCAGCATCTGCCTGGAGAAGCAGCTGCCGGTGGA
  CAAGACCGAAGACAAGTCCCTGGAGGAGCGGGGCCGCATCCTCATCTCGCTCAAGTACAGCTCACAGAAG
  CAAGGCCTGCTGGTAGGCATCGTGCGGTGCGCCCACCTGGCCGCCATGGACGCCAACGGCTACTCGGACC
  CCTACGTGAAAACATACCTGAGGCCAGATGTGGACAAGAAATCCAAACATAAGACAGCGGTGAAGAAAAA
  AACCCTGAACCCGGAGTTTAATGAGGAGTTCTGTTACGAGATCAAGCATGGGGACCTGGCCAAGAAGTCC
  CTGGAGGTCACCGTTTGGGATTACGACATTGGAAAATCCAACGATTTCATTGGTGGTGTGGTTCTGGGCA
  TCCACGCCAAGGGGGAGCGCCTGAAGCACTGGTTTGACTGCCTGAAGAACAAGGACAAGCGCATCGAGCG
  CTGGCACACGCTCACCAGCGAGCTCCCAGGGGCTGTGCTCAGCGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218949 representing NM_003585
  Red=Cloning site Green=Tags(s)
  
  MTLRRRGEKATISIQEHMAIDVCPGPIRPIKQISDYFPRFPRGLPPDAGPRAAAPPDAPARPAVAGAGRR
  SPSDGAREDDEDVDQLFGAYGSSPGPSPGPSPARPPAKPPEDEPDADGYESDDCTALGTLDFSLLYDQEN
  NALHCTITKAKGLKPMDHNGLADPYVKLHLLPGASKANKLRTKTLRNTLNPTWNETLTYYGITDEDMIRK
  TLRISVCDEDKFRHNEFIGETRVPLKKLKPNHTKTFSICLEKQLPVDKTEDKSLEERGRILISLKYSSQK
  QGLLVGIVRCAHLAAMDANGYSDPYVKTYLRPDVDKKSKHKTAVKKKTLNPEFNEEFCYEIKHGDLAKKS
  LEVTVWDYDIGKSNDFIGGVVLGIHAKGERLKHWFDCLKNKDKRIERWHTLTSELPGAVLSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003585
• ORF Size: 1236 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003585.1, NM_003585.2, NM_003585.3, NM_003585.4, NP_003576.1
• RefSeq Size: 2030
• RefSeq ORF: 1239
• Locus ID: 8447
• MW: 45.8 kDa
• Gene Summary: There are at least two protein isoforms of the Double C2 protein, namely alpha (DOC2A) and beta (DOC2B), which contain two C2-like domains. DOC2A and DOC2B are encoded by different genes; these genes are at times confused with the unrelated DAB2 gene which was initially named DOC-2. DOC2B is expressed ubiquitously and is suggested to be involved in Ca(2+)-dependent intracellular vesicle trafficking in various types of cells. [provided by RefSeq, Jul 2008]