PDE5A (NM_033430) Human Tagged ORF Clone

CAT#: RG218974

PDE5A (GFP-tagged) - Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PDE5A
• Synonyms: CGB-PDE; CN5A; PDE5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG218974 representing NM_033430
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGCCCTTTGGAGACAAAACAAGAGAAATGGTCAATGCATGGTTTGCTGAGAGAGTTCACACCATCC
  CTGTGTGCAAGGAAGGTATCAGAGGCCACACCGAATCTTGCTCTTGTCCCTTGCAGCAGAGTCCTCGTGC
  AGATAACAGTGCCCCTGGAACACCAACCAGGAAAATCTCTGCCTCTGAATTTGACCGGCCTCTTAGACCC
  ATTGTTGTCAAGGATTCTGAGGGAACTGTGAGCTTCCTCTCTGACTCAGAAAAGAAGGAACAGATGCCTC
  TAACCCCTCCAAGGTTTGATCATGATGAAGGGGACCAGTGCTCAAGACTCTTGGAATTAGTGAAGGATAT
  TTCTAGTCATTTGGATGTCACAGCCTTATGTCACAAAATTTTCTTGCATATCCATGGACTGATATCTGCT
  GACCGCTATTCCCTGTTCCTTGTCTGTGAAGACAGCTCCAATGACAAGTTTCTTATCAGCCGCCTCTTTG
  ATGTTGCTGAAGGTTCAACACTGGAAGAAGTTTCAAATAACTGTATCCGCTTAGAATGGAACAAAGGCAT
  TGTGGGACATGTGGCAGCGCTTGGTGAGCCCTTGAACATCAAAGATGCATATGAGGATCCTCGGTTCAAT
  GCAGAAGTTGACCAAATTACAGGCTACAAGACACAAAGCATTCTTTGTATGCCAATTAAGAATCATAGGG
  AAGAGGTTGTTGGTGTAGCCCAGGCCATCAACAAGAAATCAGGAAACGGTGGGACATTTACTGAAAAAGA
  TGAAAAGGACTTTGCTGCTTATTTGGCATTTTGTGGTATTGTTCTTCATAATGCTCAGCTCTATGAGACT
  TCACTGCTGGAGAACAAGAGAAATCAGGTGCTGCTTGACCTTGCTAGTTTAATTTTTGAAGAACAACAAT
  CATTAGAAGTAATTTTGAAGAAAATAGCTGCCACTATTATCTCTTTCATGCAAGTGCAGAAATGCACCAT
  TTTCATAGTGGATGAAGATTGCTCCGATTCTTTTTCTAGTGTGTTTCACATGGAGTGTGAGGAATTAGAA
  AAATCATCTGATACATTAACAAGGGAACATGATGCAAACAAAATCAATTACATGTATGCTCAGTATGTCA
  AAAATACTATGGAACCACTTAATATCCCAGATGTCAGTAAGGATAAAAGATTTCCCTGGACAACTGAAAA
  TACAGGAAATGTAAACCAGCAGTGCATTAGAAGTTTGCTTTGTACACCTATAAAAAATGGAAAGAAGAAT
  AAAGTTATAGGGGTTTGCCAACTTGTTAATAAGATGGAGGAGAATACTGGCAAGGTTAAGCCTTTCAACC
  GAAATGACGAACAGTTTCTGGAAGCTTTTGTCATCTTTTGTGGCTTGGGGATCCAGAACACGCAGATGTA
  TGAAGCAGTGGAGAGAGCCATGGCCAAGCAAATGGTCACATTGGAGGTTCTGTCGTATCATGCTTCAGCA
  GCAGAGGAAGAAACAAGAGAGCTACAGTCGTTAGCGGCTGCTGTGGTGCCATCTGCCCAGACCCTTAAAA
  TTACTGACTTTAGCTTCAGTGACTTTGAGCTGTCTGATCTGGAAACAGCACTGTGTACAATTCGGATGTT
  TACTGACCTCAACCTTGTGCAGAACTTCCAGATGAAACATGAGGTTCTTTGCAGATGGATTTTAAGTGTT
  AAGAAGAATTATCGGAAGAATGTTGCCTATCATAATTGGAGACATGCCTTTAATACAGCTCAGTGCATGT
  TTGCTGCTCTAAAAGCAGGCAAAATTCAGAACAAGCTGACTGACCTGGAGATACTTGCATTGCTGATTGC
  TGCACTAAGCCACGATTTGGATCACCGTGGTGTGAATAACTCTTACATACAGCGAAGTGAACATCCACTT
  GCCCAGCTTTACTGCCATTCAATCATGGAACACCATCATTTTGACCAGTGCCTGATGATTCTTAATAGTC
  CAGGCAATCAGATTCTCAGTGGCCTCTCCATTGAAGAATATAAGACCACGTTGAAAATAATCAAGCAAGC
  TATTTTAGCTACAGACCTAGCACTGTACATTAAGAGGCGAGGAGAATTTTTTGAACTTATAAGAAAAAAT
  CAATTCAATTTGGAAGATCCTCATCAAAAGGAGTTGTTTTTGGCAATGCTGATGACAGCTTGTGATCTTT
  CTGCAATTACAAAACCCTGGCCTATTCAACAACGGATAGCAGAACTTGTAGCAACTGAATTTTTTGATCA
  AGGAGACAGAGAGAGAAAAGAACTCAACATAGAACCCACTGATCTAATGAACAGGGAGAAGAAAAACAAA
  ATCCCAAGTATGCAAGTTGGGTTCATAGATGCCATCTGCTTGCAACTGTATGAGGCCCTGACCCACGTGT
  CAGAGGACTGTTTCCCTTTGCTAGATGGCTGCAGAAAGAACAGGCAGAAATGGCAGGCCCTTGCAGAACA
  GCAGGAGAAGATGCTGATTAATGGGGAAAGCGGCCAGGCCAAGCGGAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG218974 representing NM_033430
  Red=Cloning site Green=Tags(s)
  
  MLPFGDKTREMVNAWFAERVHTIPVCKEGIRGHTESCSCPLQQSPRADNSAPGTPTRKISASEFDRPLRP
  IVVKDSEGTVSFLSDSEKKEQMPLTPPRFDHDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISA
  DRYSLFLVCEDSSNDKFLISRLFDVAEGSTLEEVSNNCIRLEWNKGIVGHVAALGEPLNIKDAYEDPRFN
  AEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYET
  SLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELE
  KSSDTLTREHDANKINYMYAQYVKNTMEPLNIPDVSKDKRFPWTTENTGNVNQQCIRSLLCTPIKNGKKN
  KVIGVCQLVNKMEENTGKVKPFNRNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASA
  AEEETRELQSLAAAVVPSAQTLKITDFSFSDFELSDLETALCTIRMFTDLNLVQNFQMKHEVLCRWILSV
  KKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLEILALLIAALSHDLDHRGVNNSYIQRSEHPL
  AQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKN
  QFNLEDPHQKELFLAMLMTACDLSAITKPWPIQQRIAELVATEFFDQGDRERKELNIEPTDLMNREKKNK
  IPSMQVGFIDAICLQLYEALTHVSEDCFPLLDGCRKNRQKWQALAEQQEKMLINGESGQAKRN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033430
• ORF Size: 2499 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_033430.2, NP_236914.2
• RefSeq Size: 6836
• RefSeq ORF: 2502
• Locus ID: 8654
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• Gene Summary: This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]